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The map label for this gene is eda [H]

Identifier: 119946551

GI number: 119946551

Start: 3615318

End: 3615965

Strand: Reverse

Name: eda [H]

Synonym: Ping_2930

Alternate gene names: 119946551

Gene position: 3615965-3615318 (Counterclockwise)

Preceding gene: 119946552

Following gene: 119946549

Centisome position: 79.3

GC content: 48.77

Gene sequence:

>648_bases
ATGAATTGGACTCTTTCACCTGGAGAGGTCTTTGCTCACTCGCCTGTCGTACCAGTTATGGTGATTAATAATATTGAAGA
TGCGCTGCCAATGGCCAGGGCGCTAGCGGCAGGAGGAATTAATATTTTTGAAGTGACCTTAAGAACAAAGGTTGCTTTAG
AGGCGATTAAAGCTATTGCGACAGCGATGCCCGAGGCCATGATCGGTGCCGGGACTATTATCAATGCACAACAATATGAC
GCTGCCGTTGCCGCCGGTGCAAAATTTATTATCTCTCCCGGATACAGCCAGCGCTTATTAGAGCATGCCAAAACGGGATA
TGCACCTTTGATTCCAGGCGTTGCAACGCCTTCGGAAATCATTACTGCCTTAGAGCTCGGTTATGATCATCTGAAGTTTT
TCCCGGCAGAGGCGAATGGTGGCGCTGCTGCATTAAAAGCAATTGCAGGGCCCCTACCTCAGGTGCGTTTTTGCCCAACG
GGTGGAATAAGCCTGAACAACGTGGCTGATTACATGGCCTTAAATTGTGTGGCAACTGTCGGTGGTTCCTGGATGCTCCC
TAATGACGCTATTGCACAGGGTCATTGGGATAAAGTGACTAAGCTTGCCGGGCAGGCAGTGTCTTTGATGGCAAACTTAA
AGGCTTAA

Upstream 100 bases:

>100_bases
TTAATTAATAAGGAGTTAACATGAATTGGACTATTTCACCTGCAGAGGTCTTTGCTCACTCCCCGATTGTGTCAGTGATA
GTAATCAAGAAGGATTTAAT

Downstream 100 bases:

>100_bases
TTGATCAACAGTGCAGTGCTATTAAGATTATTTATGTTACAACAGGGCAGATATCATTGATTGATGCCTGTCTTGATAAC
AAAAATCAGATACTTTCACC

Product: 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MNWTLSPGEVFAHSPVVPVMVINNIEDALPMARALAAGGINIFEVTLRTKVALEAIKAIATAMPEAMIGAGTIINAQQYD
AAVAAGAKFIISPGYSQRLLEHAKTGYAPLIPGVATPSEIITALELGYDHLKFFPAEANGGAAALKAIAGPLPQVRFCPT
GGISLNNVADYMALNCVATVGGSWMLPNDAIAQGHWDKVTKLAGQAVSLMANLKA

Sequences:

>Translated_215_residues
MNWTLSPGEVFAHSPVVPVMVINNIEDALPMARALAAGGINIFEVTLRTKVALEAIKAIATAMPEAMIGAGTIINAQQYD
AAVAAGAKFIISPGYSQRLLEHAKTGYAPLIPGVATPSEIITALELGYDHLKFFPAEANGGAAALKAIAGPLPQVRFCPT
GGISLNNVADYMALNCVATVGGSWMLPNDAIAQGHWDKVTKLAGQAVSLMANLKA
>Mature_215_residues
MNWTLSPGEVFAHSPVVPVMVINNIEDALPMARALAAGGINIFEVTLRTKVALEAIKAIATAMPEAMIGAGTIINAQQYD
AAVAAGAKFIISPGYSQRLLEHAKTGYAPLIPGVATPSEIITALELGYDHLKFFPAEANGGAAALKAIAGPLPQVRFCPT
GGISLNNVADYMALNCVATVGGSWMLPNDAIAQGHWDKVTKLAGQAVSLMANLKA

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=207, Percent_Identity=53.6231884057971, Blast_Score=218, Evalue=2e-58,
Organism=Escherichia coli, GI48994954, Length=165, Percent_Identity=30.9090909090909, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 22320; Mature: 22320

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWTLSPGEVFAHSPVVPVMVINNIEDALPMARALAAGGINIFEVTLRTKVALEAIKAIA
CCCCCCCCCHHCCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHH
TAMPEAMIGAGTIINAQQYDAAVAAGAKFIISPGYSQRLLEHAKTGYAPLIPGVATPSEI
HHCCHHHHCCCEEECHHHHHHHHHHCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHH
ITALELGYDHLKFFPAEANGGAAALKAIAGPLPQVRFCPTGGISLNNVADYMALNCVATV
HHHHHHCHHHHEEECCCCCCHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHC
GGSWMLPNDAIAQGHWDKVTKLAGQAVSLMANLKA
CCCEECCCHHHCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNWTLSPGEVFAHSPVVPVMVINNIEDALPMARALAAGGINIFEVTLRTKVALEAIKAIA
CCCCCCCCCHHCCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHH
TAMPEAMIGAGTIINAQQYDAAVAAGAKFIISPGYSQRLLEHAKTGYAPLIPGVATPSEI
HHCCHHHHCCCEEECHHHHHHHHHHCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHH
ITALELGYDHLKFFPAEANGGAAALKAIAGPLPQVRFCPTGGISLNNVADYMALNCVATV
HHHHHHCHHHHEEECCCCCCHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHC
GGSWMLPNDAIAQGHWDKVTKLAGQAVSLMANLKA
CCCEECCCHHHCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8145647 [H]