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The map label for this gene is ptsN [H]

Identifier: 119946516

GI number: 119946516

Start: 3571264

End: 3571770

Strand: Direct

Name: ptsN [H]

Synonym: Ping_2893

Alternate gene names: 119946516

Gene position: 3571264-3571770 (Clockwise)

Preceding gene: 119946515

Following gene: 119946517

Centisome position: 78.32

GC content: 33.14

Gene sequence:

>507_bases
ATGATCATTCATAGCGCAAGTGCTAACATCGCGCAAATTTTTGTAATAGCTAAATCAAATATGAATATTTCAAGCTTATT
AAATAAAGAATCTGTTTTTGCCAAAACTGCTTGTACAAGCAAAAAATGCGCATTAGAAAAAATTAGCCATATTGCGGGTG
AAAAATTAAATATAGATCCGCAAATATTATTTAATCGTTTAATTGCGCGTGAAAAAATCGGCTCTACGGGTGTTGGAACG
GGTATTGCGATTCCACACGTAAAAATAGATAGCGCTTTACCCGCTACAGCTGTTTTTATACAATGTGAATCAGCAATAGA
TTACGATGCATACGATGGTAATAAAGTTGACATACTCTTTGCCATTTTTGTACCGGAAGATCGTTGTAAACAGTATTTGT
CTGCTCTTTCTGAAATCAGTCAAAAACTATTGGATAAACAATTTTTACGTCGACTAAGAAATGCGAAAAATAACGAAATG
TTATGTCTGTTGCTAGAAATTAAATAA

Upstream 100 bases:

>100_bases
GTACTATCACAAAATATCGGGAATTATTGGGGATTTCATCATCAAGCCAACGAAAAATGCTGCTCTGATTTTATAAAGAT
CAAGAAAACCATATTTGTTG

Downstream 100 bases:

>100_bases
TGGCAGTATTTGTCTGCGCTGTCTGAAATACGTCAACTAAGAAATGCGCAAAGCAACAAAATATTATCTCTATTGCTAGC
AGTTAAATAATTGCGGTAAT

Product: putative PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; PTS system EIIA component; Phosphotransferase enzyme IIA component [H]

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MIIHSASANIAQIFVIAKSNMNISSLLNKESVFAKTACTSKKCALEKISHIAGEKLNIDPQILFNRLIAREKIGSTGVGT
GIAIPHVKIDSALPATAVFIQCESAIDYDAYDGNKVDILFAIFVPEDRCKQYLSALSEISQKLLDKQFLRRLRNAKNNEM
LCLLLEIK

Sequences:

>Translated_168_residues
MIIHSASANIAQIFVIAKSNMNISSLLNKESVFAKTACTSKKCALEKISHIAGEKLNIDPQILFNRLIAREKIGSTGVGT
GIAIPHVKIDSALPATAVFIQCESAIDYDAYDGNKVDILFAIFVPEDRCKQYLSALSEISQKLLDKQFLRRLRNAKNNEM
LCLLLEIK
>Mature_168_residues
MIIHSASANIAQIFVIAKSNMNISSLLNKESVFAKTACTSKKCALEKISHIAGEKLNIDPQILFNRLIAREKIGSTGVGT
GIAIPHVKIDSALPATAVFIQCESAIDYDAYDGNKVDILFAIFVPEDRCKQYLSALSEISQKLLDKQFLRRLRNAKNNEM
LCLLLEIK

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=144, Percent_Identity=45.8333333333333, Blast_Score=138, Evalue=2e-34,
Organism=Escherichia coli, GI2367327, Length=106, Percent_Identity=29.2452830188679, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 18553; Mature: 18553

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIHSASANIAQIFVIAKSNMNISSLLNKESVFAKTACTSKKCALEKISHIAGEKLNIDP
CEEECCCCCEEEEEEEEECCCCHHHHHCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCH
QILFNRLIAREKIGSTGVGTGIAIPHVKIDSALPATAVFIQCESAIDYDAYDGNKVDILF
HHHHHHHHHHHHCCCCCCCCCEECCCEEECCCCCCEEEEEEECCCCCCCCCCCCEEEEEE
AIFVPEDRCKQYLSALSEISQKLLDKQFLRRLRNAKNNEMLCLLLEIK
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEC
>Mature Secondary Structure
MIIHSASANIAQIFVIAKSNMNISSLLNKESVFAKTACTSKKCALEKISHIAGEKLNIDP
CEEECCCCCEEEEEEEEECCCCHHHHHCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCH
QILFNRLIAREKIGSTGVGTGIAIPHVKIDSALPATAVFIQCESAIDYDAYDGNKVDILF
HHHHHHHHHHHHCCCCCCCCCEECCCEEECCCCCCEEEEEEECCCCCCCCCCCCEEEEEE
AIFVPEDRCKQYLSALSEISQKLLDKQFLRRLRNAKNNEMLCLLLEIK
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7876255; 8444818; 8025669; 9278503; 9636714 [H]