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The map label for this gene is rpoN [H]

Identifier: 119946515

GI number: 119946515

Start: 3569777

End: 3571231

Strand: Direct

Name: rpoN [H]

Synonym: Ping_2892

Alternate gene names: 119946515

Gene position: 3569777-3571231 (Clockwise)

Preceding gene: 119946514

Following gene: 119946516

Centisome position: 78.29

GC content: 39.79

Gene sequence:

>1455_bases
TTGATAAAACAAGGTTTGCATTTAAAGATGGGGCAGCAACTTGCAATGACCCCACAATTGCAGCAAGCAATCAAATTGCT
ACAACTCTCTACACTGGATTTACAGCAGGAAATTCAGAATATTCTCGACTCAAACCCGCTATTAGAACAAGAAGATAACA
ACGGTACGCAGGAAGTCATCGCAAAGCAGGATAGCAGTCAGATTGATACTTCTGAAGCAATAACCCAGCAAGAGTTCTCT
AATGATTTACCCGCTGAAAATAACTGGGAAGAATCTTATTCTGAATATTCAAGCAGCTCCACAACTTCTCCCTCATCCTC
TTCAAGTTATGAAGGCGAGGACAGTATTTACCAGGGAGAATCTTCAACCTCTTTACAAGATCATTTAATTTGGCAAATGC
AACTGACCCCATTTTCCGATACGGATCTTGTCATAGCCGCTGCCCTCATTGACTCGATAGATGAGTCTGGCTATTTAACC
GCGAGTTGTGAATCAATTTTGGAAAGCTTAATCACTGAAGCTGATGATATTGAATTAGATGAAGTACTGGTTGTCCTAAA
ACGCATTCAACATTTTGATCCAATCGGTGTTGCTGCTCGTTCAACTCAGGAGTGTTTACAAATCCAACTGCGACATCTAC
CGGAACAAACACCATGGCGCAGTGAAGCGTTAACAATACTTGCTCATCACATGGATTTACTGGGTAACCGGGATTACCGT
ACTCTGGCAAGAAAAAGTAAATTAAAAGAGTTTGAATTAGCAGAGGTATTGCATTTAATTCAAACGCTTGAACCCAGACC
TGGAAACCAAATCGAAAATAGCCAACCCCAATATATTGTGCCCGATGTTTATGTCAAAAAAATTGCAGGTCAATGGCGTG
TATCGCTCCACCCAGATTCAGATGCTAAGCTTTCCATAAATCAAACATATGCAAATTTATGTAAAGGTAATATTAGTAAA
GCTGATTCTCAGTTTATTAAAGGTCATTTACAAGAAGCAAGATGGTTTATTAAAAGCCTGGAAAGCCGTAACGAAACCTT
ATTAAAAGTCTCTCATTGTATTGTGCGCCAACAACAAGCATTCTTCGAATATGGGGAAGAATCAATGAAACCCATGGTGC
TTAATGATGTTGCGTTAGAGGTAGATATGCATGAATCGACCATTTCCCGGGTCACGACACAAAAATTCATGCATACCTCA
AGAGGTATTTTTGAATTGAAGTTTTTCTTTTCCAGCCATGTTGCGACAGAAAGTGGAGGAGAATGCTCCTCAACGGCAAT
TAAGGCATTAATCAAAAAATTAATCTCTGCAGAGGTCAGCAGCAAACCTTTAAGTGATAATAAAATAGCACTGTTACTTG
AACACCAGGGTATTAAAGTGGCGCGCCGTACTATCACAAAATATCGGGAATTATTGGGGATTTCATCATCAAGCCAACGA
AAAATGCTGCTCTGA

Upstream 100 bases:

>100_bases
AGACTTATACAGGGTATATAAAAAGCTATTTTTTAAACCAAATTCCGCATTAATAATAACTCAATAACTTATATAATCAG
CCAGATTCAGGGGGAATGTT

Downstream 100 bases:

>100_bases
TTTTATAAAGATCAAGAAAACCATATTTGTTGATGATCATTCATAGCGCAAGTGCTAACATCGCGCAAATTTTTGTAATA
GCTAAATCAAATATGAATAT

Product: RNA polymerase, sigma 54 subunit, RpoN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MIKQGLHLKMGQQLAMTPQLQQAIKLLQLSTLDLQQEIQNILDSNPLLEQEDNNGTQEVIAKQDSSQIDTSEAITQQEFS
NDLPAENNWEESYSEYSSSSTTSPSSSSSYEGEDSIYQGESSTSLQDHLIWQMQLTPFSDTDLVIAAALIDSIDESGYLT
ASCESILESLITEADDIELDEVLVVLKRIQHFDPIGVAARSTQECLQIQLRHLPEQTPWRSEALTILAHHMDLLGNRDYR
TLARKSKLKEFELAEVLHLIQTLEPRPGNQIENSQPQYIVPDVYVKKIAGQWRVSLHPDSDAKLSINQTYANLCKGNISK
ADSQFIKGHLQEARWFIKSLESRNETLLKVSHCIVRQQQAFFEYGEESMKPMVLNDVALEVDMHESTISRVTTQKFMHTS
RGIFELKFFFSSHVATESGGECSSTAIKALIKKLISAEVSSKPLSDNKIALLLEHQGIKVARRTITKYRELLGISSSSQR
KMLL

Sequences:

>Translated_484_residues
MIKQGLHLKMGQQLAMTPQLQQAIKLLQLSTLDLQQEIQNILDSNPLLEQEDNNGTQEVIAKQDSSQIDTSEAITQQEFS
NDLPAENNWEESYSEYSSSSTTSPSSSSSYEGEDSIYQGESSTSLQDHLIWQMQLTPFSDTDLVIAAALIDSIDESGYLT
ASCESILESLITEADDIELDEVLVVLKRIQHFDPIGVAARSTQECLQIQLRHLPEQTPWRSEALTILAHHMDLLGNRDYR
TLARKSKLKEFELAEVLHLIQTLEPRPGNQIENSQPQYIVPDVYVKKIAGQWRVSLHPDSDAKLSINQTYANLCKGNISK
ADSQFIKGHLQEARWFIKSLESRNETLLKVSHCIVRQQQAFFEYGEESMKPMVLNDVALEVDMHESTISRVTTQKFMHTS
RGIFELKFFFSSHVATESGGECSSTAIKALIKKLISAEVSSKPLSDNKIALLLEHQGIKVARRTITKYRELLGISSSSQR
KMLL
>Mature_484_residues
MIKQGLHLKMGQQLAMTPQLQQAIKLLQLSTLDLQQEIQNILDSNPLLEQEDNNGTQEVIAKQDSSQIDTSEAITQQEFS
NDLPAENNWEESYSEYSSSSTTSPSSSSSYEGEDSIYQGESSTSLQDHLIWQMQLTPFSDTDLVIAAALIDSIDESGYLT
ASCESILESLITEADDIELDEVLVVLKRIQHFDPIGVAARSTQECLQIQLRHLPEQTPWRSEALTILAHHMDLLGNRDYR
TLARKSKLKEFELAEVLHLIQTLEPRPGNQIENSQPQYIVPDVYVKKIAGQWRVSLHPDSDAKLSINQTYANLCKGNISK
ADSQFIKGHLQEARWFIKSLESRNETLLKVSHCIVRQQQAFFEYGEESMKPMVLNDVALEVDMHESTISRVTTQKFMHTS
RGIFELKFFFSSHVATESGGECSSTAIKALIKKLISAEVSSKPLSDNKIALLLEHQGIKVARRTITKYRELLGISSSSQR
KMLL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=483, Percent_Identity=57.1428571428571, Blast_Score=566, Evalue=1e-162,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 54529; Mature: 54529

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKQGLHLKMGQQLAMTPQLQQAIKLLQLSTLDLQQEIQNILDSNPLLEQEDNNGTQEVI
CCCCCCCHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
AKQDSSQIDTSEAITQQEFSNDLPAENNWEESYSEYSSSSTTSPSSSSSYEGEDSIYQGE
HCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
SSTSLQDHLIWQMQLTPFSDTDLVIAAALIDSIDESGYLTASCESILESLITEADDIELD
CCCCHHHHEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHH
EVLVVLKRIQHFDPIGVAARSTQECLQIQLRHLPEQTPWRSEALTILAHHMDLLGNRDYR
HHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHH
TLARKSKLKEFELAEVLHLIQTLEPRPGNQIENSQPQYIVPDVYVKKIAGQWRVSLHPDS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCHHHHHHHCCCEEEEECCCC
DAKLSINQTYANLCKGNISKADSQFIKGHLQEARWFIKSLESRNETLLKVSHCIVRQQQA
CCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
FFEYGEESMKPMVLNDVALEVDMHESTISRVTTQKFMHTSRGIFELKFFFSSHVATESGG
HHHHHHHHCCCEEEEEEEEEEECHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCC
ECSSTAIKALIKKLISAEVSSKPLSDNKIALLLEHQGIKVARRTITKYRELLGISSSSQR
CHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCC
KMLL
CCCC
>Mature Secondary Structure
MIKQGLHLKMGQQLAMTPQLQQAIKLLQLSTLDLQQEIQNILDSNPLLEQEDNNGTQEVI
CCCCCCCHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
AKQDSSQIDTSEAITQQEFSNDLPAENNWEESYSEYSSSSTTSPSSSSSYEGEDSIYQGE
HCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
SSTSLQDHLIWQMQLTPFSDTDLVIAAALIDSIDESGYLTASCESILESLITEADDIELD
CCCCHHHHEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHH
EVLVVLKRIQHFDPIGVAARSTQECLQIQLRHLPEQTPWRSEALTILAHHMDLLGNRDYR
HHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHH
TLARKSKLKEFELAEVLHLIQTLEPRPGNQIENSQPQYIVPDVYVKKIAGQWRVSLHPDS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCHHHHHHHCCCEEEEECCCC
DAKLSINQTYANLCKGNISKADSQFIKGHLQEARWFIKSLESRNETLLKVSHCIVRQQQA
CCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
FFEYGEESMKPMVLNDVALEVDMHESTISRVTTQKFMHTSRGIFELKFFFSSHVATESGG
HHHHHHHHCCCEEEEEEEEEEECHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCC
ECSSTAIKALIKKLISAEVSSKPLSDNKIALLLEHQGIKVARRTITKYRELLGISSSSQR
CHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCC
KMLL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9421909 [H]