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The map label for this gene is artJ [H]

Identifier: 119946458

GI number: 119946458

Start: 3481234

End: 3482010

Strand: Reverse

Name: artJ [H]

Synonym: Ping_2833

Alternate gene names: 119946458

Gene position: 3482010-3481234 (Counterclockwise)

Preceding gene: 119946459

Following gene: 119946457

Centisome position: 76.37

GC content: 41.18

Gene sequence:

>777_bases
ATGAAAAAAATGAGTATGTTGATTGCTCTCTTTACCTTGTCAGTTAATGTTGCACAGGCGAAACAATGGACACAGATTCG
AATTGGTGTTGAAGGTGCTTATCCGCCCTTTAGCCAAACAGAAGCTGATGGTAGCGTGACCGGATTTGATATCGATATAG
CGAATGCCTTGTGTGCTGAAATGAAGGCAGACTGTACCTTAATCAAACAAGATTGGGATGGTATTATTCCTGCATTAATG
GCACGTAAATTTGATGCGATTGTAGCAACAATGGATATTACCGAAGAGCGCAAAAAACGTGTTGCCTTTACCAATAAATA
CCAGCATATTCCGGCTCGATTTGCGGCTTTAAAAGGGACTGAGTATCAAGCATCTCCAGCGTTTATGGCGGGTAAAAAAG
TGGGTGTACAGCGTGCAACTTCGATGGATCTTTATATTTCTGATAACTACCCGGAAGCTGAAATCAAACGTTATGGCTCT
GCCGATGAAGCCTATCTGGATCTTAAAGCAGGTCGCCTAGACTATATAATGGCCGACTCAGCGGCAATTACCGGTGGCTT
ATTAGAAAAAGAAGGCGGTGATAAATTTGAACTTGTGGGTCCTAAACTAAACGATCCTAAATGGTTTGGTGAAGGTGCTG
GTATTGCCGTGCGTCAACAAGATAAAGATCTTGTTGACCAATTTAATGCTGCTATTAAAGCGATTCGTGCCGATGGGACT
TATCAAACAATTCAAGATAAATACTTTACCTTTGATGTTTATGGTAGTGAAGAATAA

Upstream 100 bases:

>100_bases
TATTTTTTCAAACCCTTCATCTGAACGTTTTAAACAATTCATCTCCTCAGTTTACTAACCCTGAAACTTCACTTTACTTT
ACTTAAATTAAGGATATAAC

Downstream 100 bases:

>100_bases
GCGCTGATTAAATGCTTATCTTCCCCCTTGTTTTGAATCTATAGGTTCAAAACGAGGCAGGACTGAAAGGATGGGAAACC
ATCCTTTTTTATTAAAAAAG

Product: ABC amino acid transporter extracellular solute-binding protein, family 3

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKKMSMLIALFTLSVNVAQAKQWTQIRIGVEGAYPPFSQTEADGSVTGFDIDIANALCAEMKADCTLIKQDWDGIIPALM
ARKFDAIVATMDITEERKKRVAFTNKYQHIPARFAALKGTEYQASPAFMAGKKVGVQRATSMDLYISDNYPEAEIKRYGS
ADEAYLDLKAGRLDYIMADSAAITGGLLEKEGGDKFELVGPKLNDPKWFGEGAGIAVRQQDKDLVDQFNAAIKAIRADGT
YQTIQDKYFTFDVYGSEE

Sequences:

>Translated_258_residues
MKKMSMLIALFTLSVNVAQAKQWTQIRIGVEGAYPPFSQTEADGSVTGFDIDIANALCAEMKADCTLIKQDWDGIIPALM
ARKFDAIVATMDITEERKKRVAFTNKYQHIPARFAALKGTEYQASPAFMAGKKVGVQRATSMDLYISDNYPEAEIKRYGS
ADEAYLDLKAGRLDYIMADSAAITGGLLEKEGGDKFELVGPKLNDPKWFGEGAGIAVRQQDKDLVDQFNAAIKAIRADGT
YQTIQDKYFTFDVYGSEE
>Mature_258_residues
MKKMSMLIALFTLSVNVAQAKQWTQIRIGVEGAYPPFSQTEADGSVTGFDIDIANALCAEMKADCTLIKQDWDGIIPALM
ARKFDAIVATMDITEERKKRVAFTNKYQHIPARFAALKGTEYQASPAFMAGKKVGVQRATSMDLYISDNYPEAEIKRYGS
ADEAYLDLKAGRLDYIMADSAAITGGLLEKEGGDKFELVGPKLNDPKWFGEGAGIAVRQQDKDLVDQFNAAIKAIRADGT
YQTIQDKYFTFDVYGSEE

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=251, Percent_Identity=39.8406374501992, Blast_Score=190, Evalue=8e-50,
Organism=Escherichia coli, GI1788649, Length=254, Percent_Identity=36.2204724409449, Blast_Score=188, Evalue=3e-49,
Organism=Escherichia coli, GI1787088, Length=251, Percent_Identity=41.0358565737052, Blast_Score=184, Evalue=4e-48,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=37.7682403433476, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1788228, Length=236, Percent_Identity=30.0847457627119, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787031, Length=246, Percent_Identity=28.8617886178862, Blast_Score=103, Evalue=8e-24,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28506; Mature: 28506

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKMSMLIALFTLSVNVAQAKQWTQIRIGVEGAYPPFSQTEADGSVTGFDIDIANALCAE
CCHHHHHHHHHHHHCCHHHCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEHHHHHHHHH
MKADCTLIKQDWDGIIPALMARKFDAIVATMDITEERKKRVAFTNKYQHIPARFAALKGT
HHCCEEEEECCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHCCHHHCCHHHHHCCCC
EYQASPAFMAGKKVGVQRATSMDLYISDNYPEAEIKRYGSADEAYLDLKAGRLDYIMADS
CCCCCCCHHCCCHHCCEEECEEEEEEECCCCHHHHHHCCCCCCEEEEEECCCEEEEEECC
AAITGGLLEKEGGDKFELVGPKLNDPKWFGEGAGIAVRQQDKDLVDQFNAAIKAIRADGT
CHHCCCCEECCCCCEEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCC
YQTIQDKYFTFDVYGSEE
CEECCCCEEEEEEECCCC
>Mature Secondary Structure
MKKMSMLIALFTLSVNVAQAKQWTQIRIGVEGAYPPFSQTEADGSVTGFDIDIANALCAE
CCHHHHHHHHHHHHCCHHHCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEHHHHHHHHH
MKADCTLIKQDWDGIIPALMARKFDAIVATMDITEERKKRVAFTNKYQHIPARFAALKGT
HHCCEEEEECCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHCCHHHCCHHHHHCCCC
EYQASPAFMAGKKVGVQRATSMDLYISDNYPEAEIKRYGSADEAYLDLKAGRLDYIMADS
CCCCCCCHHCCCHHCCEEECEEEEEEECCCCHHHHHHCCCCCCEEEEEECCCEEEEEECC
AAITGGLLEKEGGDKFELVGPKLNDPKWFGEGAGIAVRQQDKDLVDQFNAAIKAIRADGT
CHHCCCCEECCCCCEEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCC
YQTIQDKYFTFDVYGSEE
CEECCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]