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The map label for this gene is kefA [C]

Identifier: 119946454

GI number: 119946454

Start: 3477578

End: 3478462

Strand: Reverse

Name: kefA [C]

Synonym: Ping_2829

Alternate gene names: 119946454

Gene position: 3478462-3477578 (Counterclockwise)

Preceding gene: 119946455

Following gene: 119946453

Centisome position: 76.29

GC content: 40.68

Gene sequence:

>885_bases
ATGCAATTAGATGCGGTGCTTAAAATATTAAACTACCCAACGATCTCGCTCGTTGGTCAGACAATTACTGTGGGCCAATG
GCTGGTTATACCCTTGGTCTTGTGTATCGGATACTTGTTAACTAAAAGCTTTATAAATATTTTATCGAAGCGTATGCTTG
CCCGGAATATGGACCCCAATCAGATTCATCTCATTAGAAGAATTATCTATGTTTTAGCCATTTCCATTTTATTAATCACT
GTCTTAGATTTGATCAATATTCCTATTGCCGCTTTTGCCTTTCTCTCCGGTGCGATCGCAATCGGGTTTGGTTTTGGTGC
GCAAAACATTATTAATAATTTTATCAGCGGTTGGATTTTAATGTGGGAACAACCAATTCGAATTGGAGATTATTTAGAAG
TTGATAATGCCAAAGGAGTCGTGGAGGAAATTAATACCCGTTCAACAAGAATTAGACGAGTTGACGGCGTTCACCTGTTG
ATTCCTAACAGTAAATTATTAGAAAATACCGTGGTAAACAGAACACTTGTTGATCATATTATGCGTACCTCTGTGTCTGT
CGGGGTTGCCTATGGCTCACCCGCTAAACAGGTGGCAGCTTTAATTCTGCGCGCGGCTAAAGAGCAATCTATTATCTTGG
AAAATCCGCCACCCGTGGTTACTTTTGAAGATTTTGCTGACAACTCTCTGGTCTTTGAAGTCAACTTCTGGATAAATGCA
ACCGTCGATGGCAGTTTACGAGCGGTCAGAAGTAACGTCCGTTTTCGTCTTGATGAGCTCTTTGCCGAGCACAATATTGT
GGTTGCATTCCCACAACGAGATCTGCATATAGACGGCAGTTTAACGCTGCTTAAAAATCCATTAAAAGTCGCTTATGAAC
GATAA

Upstream 100 bases:

>100_bases
TATTGGATTTTTGTTGCTAATAATCATTGCCCAAGTGGTCATTTTAAAAAGAAACAAATGGTATTAAAAATTATATTTAT
CAATATATTATGGAAAAATA

Downstream 100 bases:

>100_bases
TAAAAACACCAACTGGATGCGATCACATTTGACTGCTATTATCATAAATTACAAGGTGTTATAAATCACAAGGTGTTATA
AATAATAAGGAGTTATAAAA

Product: MscS mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MQLDAVLKILNYPTISLVGQTITVGQWLVIPLVLCIGYLLTKSFINILSKRMLARNMDPNQIHLIRRIIYVLAISILLIT
VLDLINIPIAAFAFLSGAIAIGFGFGAQNIINNFISGWILMWEQPIRIGDYLEVDNAKGVVEEINTRSTRIRRVDGVHLL
IPNSKLLENTVVNRTLVDHIMRTSVSVGVAYGSPAKQVAALILRAAKEQSIILENPPPVVTFEDFADNSLVFEVNFWINA
TVDGSLRAVRSNVRFRLDELFAEHNIVVAFPQRDLHIDGSLTLLKNPLKVAYER

Sequences:

>Translated_294_residues
MQLDAVLKILNYPTISLVGQTITVGQWLVIPLVLCIGYLLTKSFINILSKRMLARNMDPNQIHLIRRIIYVLAISILLIT
VLDLINIPIAAFAFLSGAIAIGFGFGAQNIINNFISGWILMWEQPIRIGDYLEVDNAKGVVEEINTRSTRIRRVDGVHLL
IPNSKLLENTVVNRTLVDHIMRTSVSVGVAYGSPAKQVAALILRAAKEQSIILENPPPVVTFEDFADNSLVFEVNFWINA
TVDGSLRAVRSNVRFRLDELFAEHNIVVAFPQRDLHIDGSLTLLKNPLKVAYER
>Mature_294_residues
MQLDAVLKILNYPTISLVGQTITVGQWLVIPLVLCIGYLLTKSFINILSKRMLARNMDPNQIHLIRRIIYVLAISILLIT
VLDLINIPIAAFAFLSGAIAIGFGFGAQNIINNFISGWILMWEQPIRIGDYLEVDNAKGVVEEINTRSTRIRRVDGVHLL
IPNSKLLENTVVNRTLVDHIMRTSVSVGVAYGSPAKQVAALILRAAKEQSIILENPPPVVTFEDFADNSLVFEVNFWINA
TVDGSLRAVRSNVRFRLDELFAEHNIVVAFPQRDLHIDGSLTLLKNPLKVAYER

Specific function: Unknown

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=261, Percent_Identity=29.8850574712644, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI2367355, Length=253, Percent_Identity=29.2490118577075, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789291, Length=244, Percent_Identity=24.1803278688525, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1787591, Length=287, Percent_Identity=27.1777003484321, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 32861; Mature: 32861

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLDAVLKILNYPTISLVGQTITVGQWLVIPLVLCIGYLLTKSFINILSKRMLARNMDPN
CCHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
QIHLIRRIIYVLAISILLITVLDLINIPIAAFAFLSGAIAIGFGFGAQNIINNFISGWIL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEECCCHHHHHHHHHHHEEE
MWEQPIRIGDYLEVDNAKGVVEEINTRSTRIRRVDGVHLLIPNSKLLENTVVNRTLVDHI
EECCCCCCCCEEEECCCCHHHHHHCCHHHHEEEECCEEEEECCCHHHHHHHHHHHHHHHH
MRTSVSVGVAYGSPAKQVAALILRAAKEQSIILENPPPVVTFEDFADNSLVFEVNFWINA
HHHHHHEEEECCCCHHHHHHHHHHHHCCCCEEECCCCCEEEECCCCCCEEEEEEEEEEEE
TVDGSLRAVRSNVRFRLDELFAEHNIVVAFPQRDLHIDGSLTLLKNPLKVAYER
EECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCHHHHCCCHHHHCCC
>Mature Secondary Structure
MQLDAVLKILNYPTISLVGQTITVGQWLVIPLVLCIGYLLTKSFINILSKRMLARNMDPN
CCHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
QIHLIRRIIYVLAISILLITVLDLINIPIAAFAFLSGAIAIGFGFGAQNIINNFISGWIL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEECCCHHHHHHHHHHHEEE
MWEQPIRIGDYLEVDNAKGVVEEINTRSTRIRRVDGVHLLIPNSKLLENTVVNRTLVDHI
EECCCCCCCCEEEECCCCHHHHHHCCHHHHEEEECCEEEEECCCHHHHHHHHHHHHHHHH
MRTSVSVGVAYGSPAKQVAALILRAAKEQSIILENPPPVVTFEDFADNSLVFEVNFWINA
HHHHHHEEEECCCCHHHHHHHHHHHHCCCCEEECCCCCEEEECCCCCCEEEEEEEEEEEE
TVDGSLRAVRSNVRFRLDELFAEHNIVVAFPQRDLHIDGSLTLLKNPLKVAYER
EECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]