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The map label for this gene is rimK

Identifier: 119946453

GI number: 119946453

Start: 3476308

End: 3477216

Strand: Reverse

Name: rimK

Synonym: Ping_2828

Alternate gene names: 119946453

Gene position: 3477216-3476308 (Counterclockwise)

Preceding gene: 119946454

Following gene: 119946452

Centisome position: 76.26

GC content: 42.46

Gene sequence:

>909_bases
ATGAAAATTGTTATTTTATCAAGAAATAAAAGTTTGTATTCGACAAGAAGATTAAAAGAAGAAGGCGAAGCCCGAGGTCA
TCAGATTGATATTATTGATACGCTGCATTGTTATATGGATATAACCAGCAGCCGCCCAACGGTTCGCTATCAGGGTGAAG
AATTACCCGTCTATGATGCACTTATTCCGCGGATCGGCGCCTCTGTTACTTTTTACGGGACAGCCGTTGCCCGCCAATTT
GAAGTAATGGGGACATTTAATATCAATGAATCGGTAGCAATTAGCCGTTCAAGAGATAAATTACGTTCAATGCAATTATT
ATCACGTAAAGGCATCGGTATGCCAAGAACGGGCTTTGCCAGTAAACCTGATAATATTAAAGACTTGATTAAAAATGTCG
GTGGCGCACCTGTTGTTATCAAATTGCTAGAAGGTACTCAGGGGATTGGCGTAGTGCTGGCTGAAACCGCTAAAACAGCT
GAAAGTATTATCGAAGCTTTTATGGGGATCAAAGCCAACATTTTGGTACAGGAGTTTATTAAAGAAGCCGGTGGTGCAGA
TATTCGCTGTCTGGTCATTGGCGGTAAAGTTGTTGCGGCAATGAAAAGGCAGGGTGCTGAGGGTGAATTTCGTTCTAACC
TGCATCGTGGTGGGTCTGCTGTGGTGGTTAAATTAACAAAAATAGAGCGTGAAACTGCCGTTAATGCGGCGAAAATAATG
GGTTTAAATTTTTGTGGTGTGGACTTGTTACGCTCGGAAAGTGGCCCTAAGGTGATGGAAGTGAATTCCTCTCCTGGTTT
AGAAGGTATTGAGACGGCTACCGGCAAAAATATTGCGGGTATGGTATTCGAATTTCTCGAAAAAAATATGAAAGCTGAGC
ATTCGAATAAAACCCGTGGACGTGGCTGA

Upstream 100 bases:

>100_bases
ATGTTTTCACCTAAACTCGCGGAAATTTAACGAGGGTGGGCTATCCTTCATAAAACAAAAGTAATGGATTTAAGTTGAAG
CTGATTTCAAAGGAAAAATT

Downstream 100 bases:

>100_bases
TTAAGGATTGATCATGGCGGTATTACGCATTGGCGATTTTGATATTTTACCTGGAACAGATCAAAAAATAGATCTCCCTG
TCGCAAAGTTGTACACAGAT

Product: alpha-L-glutamate ligases, ribosomal protein S6 modification protein of RimK family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKIVILSRNKSLYSTRRLKEEGEARGHQIDIIDTLHCYMDITSSRPTVRYQGEELPVYDALIPRIGASVTFYGTAVARQF
EVMGTFNINESVAISRSRDKLRSMQLLSRKGIGMPRTGFASKPDNIKDLIKNVGGAPVVIKLLEGTQGIGVVLAETAKTA
ESIIEAFMGIKANILVQEFIKEAGGADIRCLVIGGKVVAAMKRQGAEGEFRSNLHRGGSAVVVKLTKIERETAVNAAKIM
GLNFCGVDLLRSESGPKVMEVNSSPGLEGIETATGKNIAGMVFEFLEKNMKAEHSNKTRGRG

Sequences:

>Translated_302_residues
MKIVILSRNKSLYSTRRLKEEGEARGHQIDIIDTLHCYMDITSSRPTVRYQGEELPVYDALIPRIGASVTFYGTAVARQF
EVMGTFNINESVAISRSRDKLRSMQLLSRKGIGMPRTGFASKPDNIKDLIKNVGGAPVVIKLLEGTQGIGVVLAETAKTA
ESIIEAFMGIKANILVQEFIKEAGGADIRCLVIGGKVVAAMKRQGAEGEFRSNLHRGGSAVVVKLTKIERETAVNAAKIM
GLNFCGVDLLRSESGPKVMEVNSSPGLEGIETATGKNIAGMVFEFLEKNMKAEHSNKTRGRG
>Mature_302_residues
MKIVILSRNKSLYSTRRLKEEGEARGHQIDIIDTLHCYMDITSSRPTVRYQGEELPVYDALIPRIGASVTFYGTAVARQF
EVMGTFNINESVAISRSRDKLRSMQLLSRKGIGMPRTGFASKPDNIKDLIKNVGGAPVVIKLLEGTQGIGVVLAETAKTA
ESIIEAFMGIKANILVQEFIKEAGGADIRCLVIGGKVVAAMKRQGAEGEFRSNLHRGGSAVVVKLTKIERETAVNAAKIM
GLNFCGVDLLRSESGPKVMEVNSSPGLEGIETATGKNIAGMVFEFLEKNMKAEHSNKTRGRG

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI164663810, Length=213, Percent_Identity=30.5164319248826, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI134254462, Length=213, Percent_Identity=29.5774647887324, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1787076, Length=300, Percent_Identity=59, Blast_Score=377, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMK_PSYIN (A1SYG9)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944133.1
- STRING:   A1SYG9
- GeneID:   4626272
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_2828
- eggNOG:   COG0189
- HOGENOM:   HBG679591
- OMA:   CREPRLY
- PhylomeDB:   A1SYG9
- BioCyc:   PING357804:PING_2828-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_01552
- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- TIGRFAMs:   TIGR00768

Pfam domain/function: PF08443 RimK

EC number: NA

Molecular weight: Translated: 32772; Mature: 32772

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVILSRNKSLYSTRRLKEEGEARGHQIDIIDTLHCYMDITSSRPTVRYQGEELPVYDA
CEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEHEEEEEEECCCCCEEEECCCCCCHHHH
LIPRIGASVTFYGTAVARQFEVMGTFNINESVAISRSRDKLRSMQLLSRKGIGMPRTGFA
HHHHHCCCEEEEHHHHHHEEEEEEEECCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCC
SKPDNIKDLIKNVGGAPVVIKLLEGTQGIGVVLAETAKTAESIIEAFMGIKANILVQEFI
CCCCCHHHHHHHCCCCCEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHH
KEAGGADIRCLVIGGKVVAAMKRQGAEGEFRSNLHRGGSAVVVKLTKIERETAVNAAKIM
HHCCCCCEEEEEECCHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHE
GLNFCGVDLLRSESGPKVMEVNSSPGLEGIETATGKNIAGMVFEFLEKNMKAEHSNKTRG
ECCCCCHHHHHCCCCCEEEEECCCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCCCCCCCC
RG
CC
>Mature Secondary Structure
MKIVILSRNKSLYSTRRLKEEGEARGHQIDIIDTLHCYMDITSSRPTVRYQGEELPVYDA
CEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEHEEEEEEECCCCCEEEECCCCCCHHHH
LIPRIGASVTFYGTAVARQFEVMGTFNINESVAISRSRDKLRSMQLLSRKGIGMPRTGFA
HHHHHCCCEEEEHHHHHHEEEEEEEECCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCC
SKPDNIKDLIKNVGGAPVVIKLLEGTQGIGVVLAETAKTAESIIEAFMGIKANILVQEFI
CCCCCHHHHHHHCCCCCEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHH
KEAGGADIRCLVIGGKVVAAMKRQGAEGEFRSNLHRGGSAVVVKLTKIERETAVNAAKIM
HHCCCCCEEEEEECCHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHE
GLNFCGVDLLRSESGPKVMEVNSSPGLEGIETATGKNIAGMVFEFLEKNMKAEHSNKTRG
ECCCCCHHHHHCCCCCEEEEECCCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCCCCCCCC
RG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA