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The map label for this gene is purR [H]

Identifier: 119946411

GI number: 119946411

Start: 3427424

End: 3428476

Strand: Reverse

Name: purR [H]

Synonym: Ping_2785

Alternate gene names: 119946411

Gene position: 3428476-3427424 (Counterclockwise)

Preceding gene: 119946412

Following gene: 119946410

Centisome position: 75.19

GC content: 34.0

Gene sequence:

>1053_bases
ATGCCTCATATGAATAAAAAGACGACGTTAAAAGATGTCGCCAGCGAGCTTGGAATTAGCAGCTCAACGGTCTCAAGGGC
GTTAAACGAAAAGACAAAAAGCATGATCAATGCAGCGCTTGTTTTGAAAATTGAGAAAACCGCTGCAAAAATGGAATACA
GCATTAATCATATAGCCCGTAGTTTGAAAAATGGGAAAAAGTACACGATTGGAATAATTATTCCCGATTTAACAAATCCT
GTATTTGGTGAAATATCCGATGAAATAATTGAATGTCTTGCAAGTTCTGAATACAAAGCGATTATCATTTTTGCACAATC
TAACTCAAGTGAATATCTTAAAAACATAAAATATATCGCTAGCAACCAAGTTGATGGGTTTATTTTTACCAATGCATATA
TAGAGGACATTGGTGTTAAGTATTGTAAGGATCATCGTATTCCCTGCGTCACTGTTGGTAGAACAACAGTGAACAAAGAC
ATCAGTCAAATTTATACTGACAATTGCCAAGGCATGAAATTAATCATTGAGCATTTAGTCGGCTTAGGTCATGAAAATAT
TGCTTTTATTGCTGGGCCACAAGATATTTCTGATGGCTACGAGCGACTTAAATATTTTGAACAATGGATGAAATTTTATA
AAGCCAATTATTGTGCCAAATCTATCATTAATACTGAAAAATTCAGCCGTTACTCAGGTTATAAAGCGGCAGATAAATTG
TTTAATAAAAAATTAAGATTTACCGCAATTGTTGCAGCCAATGATCTCTTGGCGGTTGGTATTATGGATTATTTAAAAGA
GCACGACATAATATGTCCCAGAGAGGTATCCTTGGTTGGTTTTAATGCAATGCTATTTAGTGATGTATTTTCACCACCTT
TAACCAGTGTTTATGTCGATAGAAAATTAATGGCGAAACAATCTGTTTTATATTTACTCGACGCTTTAAACAAGAAAAAT
GATGCCATTAATAAAGTCAAACTAAATATCGTTGAACCTAAATTAATCATTAGAGATTCAACTTGTCCACCTAGAAAAAT
CGCAATGAACTGA

Upstream 100 bases:

>100_bases
TGGAGATATACATAATAAAGGCTTAAAAAGATCGACCGTGGTGAATAAATTAAGCGCTGCCGCGACAGCATGTGGTTGCA
GTAAAGGAAACAAACTTTAG

Downstream 100 bases:

>100_bases
ATTTAGTTAGTCATCTTACAATATTTCATCAGGAGAAATTTACATGGTAGAGCCTAAAGTGACACAAGAGAAAGATCTTG
AACCTAATTGGAACTGGGAC

Product: transcriptional regulator of LacI family protein

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MPHMNKKTTLKDVASELGISSSTVSRALNEKTKSMINAALVLKIEKTAAKMEYSINHIARSLKNGKKYTIGIIIPDLTNP
VFGEISDEIIECLASSEYKAIIIFAQSNSSEYLKNIKYIASNQVDGFIFTNAYIEDIGVKYCKDHRIPCVTVGRTTVNKD
ISQIYTDNCQGMKLIIEHLVGLGHENIAFIAGPQDISDGYERLKYFEQWMKFYKANYCAKSIINTEKFSRYSGYKAADKL
FNKKLRFTAIVAANDLLAVGIMDYLKEHDIICPREVSLVGFNAMLFSDVFSPPLTSVYVDRKLMAKQSVLYLLDALNKKN
DAINKVKLNIVEPKLIIRDSTCPPRKIAMN

Sequences:

>Translated_350_residues
MPHMNKKTTLKDVASELGISSSTVSRALNEKTKSMINAALVLKIEKTAAKMEYSINHIARSLKNGKKYTIGIIIPDLTNP
VFGEISDEIIECLASSEYKAIIIFAQSNSSEYLKNIKYIASNQVDGFIFTNAYIEDIGVKYCKDHRIPCVTVGRTTVNKD
ISQIYTDNCQGMKLIIEHLVGLGHENIAFIAGPQDISDGYERLKYFEQWMKFYKANYCAKSIINTEKFSRYSGYKAADKL
FNKKLRFTAIVAANDLLAVGIMDYLKEHDIICPREVSLVGFNAMLFSDVFSPPLTSVYVDRKLMAKQSVLYLLDALNKKN
DAINKVKLNIVEPKLIIRDSTCPPRKIAMN
>Mature_349_residues
PHMNKKTTLKDVASELGISSSTVSRALNEKTKSMINAALVLKIEKTAAKMEYSINHIARSLKNGKKYTIGIIIPDLTNPV
FGEISDEIIECLASSEYKAIIIFAQSNSSEYLKNIKYIASNQVDGFIFTNAYIEDIGVKYCKDHRIPCVTVGRTTVNKDI
SQIYTDNCQGMKLIIEHLVGLGHENIAFIAGPQDISDGYERLKYFEQWMKFYKANYCAKSIINTEKFSRYSGYKAADKLF
NKKLRFTAIVAANDLLAVGIMDYLKEHDIICPREVSLVGFNAMLFSDVFSPPLTSVYVDRKLMAKQSVLYLLDALNKKND
AINKVKLNIVEPKLIIRDSTCPPRKIAMN

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=336, Percent_Identity=24.702380952381, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790369, Length=338, Percent_Identity=25.4437869822485, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1787948, Length=343, Percent_Identity=25.3644314868805, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1786540, Length=345, Percent_Identity=27.536231884058, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789202, Length=337, Percent_Identity=24.9258160237389, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789068, Length=355, Percent_Identity=25.0704225352113, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1788474, Length=301, Percent_Identity=24.9169435215947, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1787906, Length=260, Percent_Identity=23.8461538461538, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787580, Length=325, Percent_Identity=24, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI48994940, Length=322, Percent_Identity=23.9130434782609, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790715, Length=326, Percent_Identity=21.1656441717791, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786268, Length=331, Percent_Identity=20.5438066465257, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790689, Length=271, Percent_Identity=22.8782287822878, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 39402; Mature: 39271

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHMNKKTTLKDVASELGISSSTVSRALNEKTKSMINAALVLKIEKTAAKMEYSINHIAR
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHH
SLKNGKKYTIGIIIPDLTNPVFGEISDEIIECLASSEYKAIIIFAQSNSSEYLKNIKYIA
HHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHH
SNQVDGFIFTNAYIEDIGVKYCKDHRIPCVTVGRTTVNKDISQIYTDNCQGMKLIIEHLV
CCCCCEEEEEHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHH
GLGHENIAFIAGPQDISDGYERLKYFEQWMKFYKANYCAKSIINTEKFSRYSGYKAADKL
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FNKKLRFTAIVAANDLLAVGIMDYLKEHDIICPREVSLVGFNAMLFSDVFSPPLTSVYVD
HCCCHHEEEEEECCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHCCCCHHHHHHH
RKLMAKQSVLYLLDALNKKNDAINKVKLNIVEPKLIIRDSTCPPRKIAMN
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
PHMNKKTTLKDVASELGISSSTVSRALNEKTKSMINAALVLKIEKTAAKMEYSINHIAR
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHH
SLKNGKKYTIGIIIPDLTNPVFGEISDEIIECLASSEYKAIIIFAQSNSSEYLKNIKYIA
HHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHH
SNQVDGFIFTNAYIEDIGVKYCKDHRIPCVTVGRTTVNKDISQIYTDNCQGMKLIIEHLV
CCCCCEEEEEHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHH
GLGHENIAFIAGPQDISDGYERLKYFEQWMKFYKANYCAKSIINTEKFSRYSGYKAADKL
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FNKKLRFTAIVAANDLLAVGIMDYLKEHDIICPREVSLVGFNAMLFSDVFSPPLTSVYVD
HCCCHHEEEEEECCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHCCCCHHHHHHH
RKLMAKQSVLYLLDALNKKNDAINKVKLNIVEPKLIIRDSTCPPRKIAMN
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA