Click here to switch to the map view.

The map label for this gene is yafC [H]

Identifier: 119946301

GI number: 119946301

Start: 3271177

End: 3272058

Strand: Direct

Name: yafC [H]

Synonym: Ping_2669

Alternate gene names: 119946301

Gene position: 3271177-3272058 (Clockwise)

Preceding gene: 119946289

Following gene: 119946302

Centisome position: 71.74

GC content: 43.54

Gene sequence:

>882_bases
ATGTTAAAACCTGAGATATTGGCATTTTTAGCGGTGGTCAAAACGGGCAGTTTTACCCGTGCAGCAGAGCAGACAAAAAC
ATCAAAATCCCGCGTATCACAACAAGTATCGAGCTTAGAGAAAACCCTCGGGATCACTCTGTTACATAGATCAACCCGTA
AAATACGACTAACAGATGCGGGTGACGCTTATTACAGCGAATGCTCCCGAGCCGCCGCTATTTTAGAACAAGCCAGAAAA
AACCTTAATGAGCGGCAAGACCAGGTAGCCGGGCTGATTCGGCTCAATTCAGTCGGCGGTTTATTCTCAGAAAAGATGCT
TGCGCCTGCTGTTATTGCTTTTATGAAACAATACCCACAAGTTGAAATACAACTCGATTTTAGCAGTGCACAAGTTGATG
TTATTGCTGAAAGCGTTGACTTAGTAGTACGGATGGGACAATTAAATAATTCATCGCTTATTGCGCGTAAGTTAATGGAG
ATAAAAACCGATGTCGTGGCCAGCCCTGCGTTTATCAAAAAACAGGGAAAACCCAATCACCCGCAACAATTATCCCAGTT
CAATTGTTTATGCGGCAGCATACGAAAATGGCACTTCCAGCATAAAGGTTTAGAAAAAGCGCAGGATGTCACAGTAACAG
GCTCACTCTCCTCGGCCAACGGTCATATCTTATCGATGGCAGCTATTGAAGGGTTAGGAATAGTGCGTTTAAACGAACTT
TACACTCACCAATATCTGCAAACCGGCCAATTAATACCCGTCTTTGAACAATGGCATATCCCGGCGGTTCCGGTCTCGCT
TATTTACCCCAAGGTGCGTTATAAAACCAAACGCATCCAAGTATTTGTCGATTTTCTGATCGATTGGTTTGAGAATAAAT
AA

Upstream 100 bases:

>100_bases
TCACTATTGTAATAAATCGCGCAATGTTTATCATCAGCTATACAATCAACTCACTGTTTAATTTTATTTAACAATTAAAA
CTAAGAATAGAAGATAATTT

Downstream 100 bases:

>100_bases
GGATAATAGCAACGGAATACAGTCCAAACATGATCTCAGGCGAAAAGGCTTGAAGGCTCGATGCTCGAAACTAGAAAACT
CGAAGCTCAACACCCGAAGC

Product: transcriptional regulator, substrate-binding of LysR family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MLKPEILAFLAVVKTGSFTRAAEQTKTSKSRVSQQVSSLEKTLGITLLHRSTRKIRLTDAGDAYYSECSRAAAILEQARK
NLNERQDQVAGLIRLNSVGGLFSEKMLAPAVIAFMKQYPQVEIQLDFSSAQVDVIAESVDLVVRMGQLNNSSLIARKLME
IKTDVVASPAFIKKQGKPNHPQQLSQFNCLCGSIRKWHFQHKGLEKAQDVTVTGSLSSANGHILSMAAIEGLGIVRLNEL
YTHQYLQTGQLIPVFEQWHIPAVPVSLIYPKVRYKTKRIQVFVDFLIDWFENK

Sequences:

>Translated_293_residues
MLKPEILAFLAVVKTGSFTRAAEQTKTSKSRVSQQVSSLEKTLGITLLHRSTRKIRLTDAGDAYYSECSRAAAILEQARK
NLNERQDQVAGLIRLNSVGGLFSEKMLAPAVIAFMKQYPQVEIQLDFSSAQVDVIAESVDLVVRMGQLNNSSLIARKLME
IKTDVVASPAFIKKQGKPNHPQQLSQFNCLCGSIRKWHFQHKGLEKAQDVTVTGSLSSANGHILSMAAIEGLGIVRLNEL
YTHQYLQTGQLIPVFEQWHIPAVPVSLIYPKVRYKTKRIQVFVDFLIDWFENK
>Mature_293_residues
MLKPEILAFLAVVKTGSFTRAAEQTKTSKSRVSQQVSSLEKTLGITLLHRSTRKIRLTDAGDAYYSECSRAAAILEQARK
NLNERQDQVAGLIRLNSVGGLFSEKMLAPAVIAFMKQYPQVEIQLDFSSAQVDVIAESVDLVVRMGQLNNSSLIARKLME
IKTDVVASPAFIKKQGKPNHPQQLSQFNCLCGSIRKWHFQHKGLEKAQDVTVTGSLSSANGHILSMAAIEGLGIVRLNEL
YTHQYLQTGQLIPVFEQWHIPAVPVSLIYPKVRYKTKRIQVFVDFLIDWFENK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=32.4137931034483, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=31.2714776632302, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1789440, Length=300, Percent_Identity=28.3333333333333, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI145693193, Length=283, Percent_Identity=26.8551236749117, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1789639, Length=284, Percent_Identity=29.2253521126761, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=28.2758620689655, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787128, Length=286, Percent_Identity=25.1748251748252, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1789173, Length=248, Percent_Identity=27.4193548387097, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI157672245, Length=125, Percent_Identity=38.4, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=24.3150684931507, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787601, Length=146, Percent_Identity=32.1917808219178, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788706, Length=263, Percent_Identity=24.7148288973384, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788481, Length=256, Percent_Identity=28.125, Blast_Score=61, Evalue=7e-11,
Organism=Escherichia coli, GI1790011, Length=299, Percent_Identity=21.0702341137124, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32813; Mature: 32813

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKPEILAFLAVVKTGSFTRAAEQTKTSKSRVSQQVSSLEKTLGITLLHRSTRKIRLTDA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECC
GDAYYSECSRAAAILEQARKNLNERQDQVAGLIRLNSVGGLFSEKMLAPAVIAFMKQYPQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCC
VEIQLDFSSAQVDVIAESVDLVVRMGQLNNSSLIARKLMEIKTDVVASPAFIKKQGKPNH
EEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCC
PQQLSQFNCLCGSIRKWHFQHKGLEKAQDVTVTGSLSSANGHILSMAAIEGLGIVRLNEL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHCEEEEECCCCCCCCEEEEEHHCCCCEEEHHHH
YTHQYLQTGQLIPVFEQWHIPAVPVSLIYPKVRYKTKRIQVFVDFLIDWFENK
HHHHHHHCCCCCCHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKPEILAFLAVVKTGSFTRAAEQTKTSKSRVSQQVSSLEKTLGITLLHRSTRKIRLTDA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECC
GDAYYSECSRAAAILEQARKNLNERQDQVAGLIRLNSVGGLFSEKMLAPAVIAFMKQYPQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCC
VEIQLDFSSAQVDVIAESVDLVVRMGQLNNSSLIARKLMEIKTDVVASPAFIKKQGKPNH
EEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCC
PQQLSQFNCLCGSIRKWHFQHKGLEKAQDVTVTGSLSSANGHILSMAAIEGLGIVRLNEL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHCEEEEECCCCCCCCEEEEEHHCCCCEEEHHHH
YTHQYLQTGQLIPVFEQWHIPAVPVSLIYPKVRYKTKRIQVFVDFLIDWFENK
HHHHHHHCCCCCCHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]