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The map label for this gene is estA [H]

Identifier: 119946246

GI number: 119946246

Start: 3202949

End: 3203614

Strand: Direct

Name: estA [H]

Synonym: Ping_2608

Alternate gene names: 119946246

Gene position: 3202949-3203614 (Clockwise)

Preceding gene: 119946245

Following gene: 119946247

Centisome position: 70.25

GC content: 40.84

Gene sequence:

>666_bases
ATGAATTATTTACCTTGTGCAGAAGTACAACCCAAATTAACAGCCAATGCCGCCGTTATCTGGTTACACGGTTTAGGCGC
AGACGGACATGATTTTGAAGCCATTGTCCCAGAATTAAATTTACCCGAAGACTTAGCTATTCGTTTTATATTTCCTCACG
CACCATCGATACCGGTTACTATTAATAGTGGTTTAAAAATGCCAGCCTGGTACGATATTTTAGAGATGAGCATCGAACGC
CAAGTTGATTTAAATGGCTTAAACAGTTCAGCCAAATTGATTCAAGCATTAATAGATCGAGAAATTGAACGTGGGATCCC
AGCCAGCAGAATAGTTATCGCGGGCTTTTCCCAAGGGGGCGCAGTGGCCTATCAGGCAGCACTAACCTATGCGCAACCCT
TGGCTGGACTATTGACAATGTCTACTTATTTTGCAACTAAAGACAGTATTAAACTTAGCGAAAAAAATAAAAACCTTAAT
ATTGAGATCATGCATGGCAGCCGGGATCCTGTGGTGGATCCTAGCTTGGGAAAACAGGCATTAGACTCGCTAACCAAAAT
GGGATTTCAACCAAGTTATAAAAGTTATACAATGGAGCACTCTGTTTGTGCTGCACAAATTACAGATATTTCTGCTTGGT
TAGTTGGGTTATTAACCCCTAAATAA

Upstream 100 bases:

>100_bases
AAAATTGCAAAAGATAAATAGGGCCTAAGATAGCCAAAAAGTATTTTTATAGCCATAACATTGTTATATTATCAATCCAT
TATTTTAAAGAATAAACGTT

Downstream 100 bases:

>100_bases
AAACCAGCTAAACATAAAACATTAAGGTTTAAAGATGGCAAGACAAGTAACATATGAGTTTAAAGGCAAGCGTAAGACTA
TTCAATTTTCATACAATCAA

Product: carboxylesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MNYLPCAEVQPKLTANAAVIWLHGLGADGHDFEAIVPELNLPEDLAIRFIFPHAPSIPVTINSGLKMPAWYDILEMSIER
QVDLNGLNSSAKLIQALIDREIERGIPASRIVIAGFSQGGAVAYQAALTYAQPLAGLLTMSTYFATKDSIKLSEKNKNLN
IEIMHGSRDPVVDPSLGKQALDSLTKMGFQPSYKSYTMEHSVCAAQITDISAWLVGLLTPK

Sequences:

>Translated_221_residues
MNYLPCAEVQPKLTANAAVIWLHGLGADGHDFEAIVPELNLPEDLAIRFIFPHAPSIPVTINSGLKMPAWYDILEMSIER
QVDLNGLNSSAKLIQALIDREIERGIPASRIVIAGFSQGGAVAYQAALTYAQPLAGLLTMSTYFATKDSIKLSEKNKNLN
IEIMHGSRDPVVDPSLGKQALDSLTKMGFQPSYKSYTMEHSVCAAQITDISAWLVGLLTPK
>Mature_221_residues
MNYLPCAEVQPKLTANAAVIWLHGLGADGHDFEAIVPELNLPEDLAIRFIFPHAPSIPVTINSGLKMPAWYDILEMSIER
QVDLNGLNSSAKLIQALIDREIERGIPASRIVIAGFSQGGAVAYQAALTYAQPLAGLLTMSTYFATKDSIKLSEKNKNLN
IEIMHGSRDPVVDPSLGKQALDSLTKMGFQPSYKSYTMEHSVCAAQITDISAWLVGLLTPK

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI5453722, Length=213, Percent_Identity=37.0892018779343, Blast_Score=140, Evalue=7e-34,
Organism=Homo sapiens, GI9966764, Length=211, Percent_Identity=36.9668246445498, Blast_Score=137, Evalue=9e-33,
Organism=Homo sapiens, GI20270341, Length=214, Percent_Identity=25.2336448598131, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17508057, Length=211, Percent_Identity=36.0189573459716, Blast_Score=136, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6323147, Length=220, Percent_Identity=32.2727272727273, Blast_Score=106, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45553055, Length=212, Percent_Identity=33.9622641509434, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI45553057, Length=212, Percent_Identity=33.9622641509434, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI21357257, Length=212, Percent_Identity=33.9622641509434, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI161078179, Length=212, Percent_Identity=29.7169811320755, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24051; Mature: 24051

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYLPCAEVQPKLTANAAVIWLHGLGADGHDFEAIVPELNLPEDLAIRFIFPHAPSIPVT
CCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEECCCCCCCHHHEEEEEECCCCCCCEE
INSGLKMPAWYDILEMSIERQVDLNGLNSSAKLIQALIDREIERGIPASRIVIAGFSQGG
ECCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
AVAYQAALTYAQPLAGLLTMSTYFATKDSIKLSEKNKNLNIEIMHGSRDPVVDPSLGKQA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEECCCCCCCCCCCCHHH
LDSLTKMGFQPSYKSYTMEHSVCAAQITDISAWLVGLLTPK
HHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNYLPCAEVQPKLTANAAVIWLHGLGADGHDFEAIVPELNLPEDLAIRFIFPHAPSIPVT
CCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEECCCCCCCHHHEEEEEECCCCCCCEE
INSGLKMPAWYDILEMSIERQVDLNGLNSSAKLIQALIDREIERGIPASRIVIAGFSQGG
ECCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
AVAYQAALTYAQPLAGLLTMSTYFATKDSIKLSEKNKNLNIEIMHGSRDPVVDPSLGKQA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEECCCCCCCCCCCCHHH
LDSLTKMGFQPSYKSYTMEHSVCAAQITDISAWLVGLLTPK
HHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA