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The map label for this gene is rnt [H]

Identifier: 119946100

GI number: 119946100

Start: 2996651

End: 2997274

Strand: Reverse

Name: rnt [H]

Synonym: Ping_2450

Alternate gene names: 119946100

Gene position: 2997274-2996651 (Counterclockwise)

Preceding gene: 119946101

Following gene: 119946098

Centisome position: 65.74

GC content: 40.87

Gene sequence:

>624_bases
ATGGAAAACTTATTGGACAAGCGTTTTCGAGGCTTTTTTCCGGTCATTATTGATATTGAAACTTCTGGTCTTAATGCGCA
AACTGATGCTATTTTAGAAATAGCGGCGATTTTACCAGCGATGGATTCACAAGGTTATCTTTTTGCTGCTGAAAAAATTC
ATTTTAATGTGTTGCCTTTTGAAGGTGCAAATTTAGATCCGAAAGCCTTAGCCTTTACCGGTATTGATCCTTTTAATCCT
CTGCGTGCTGCGGTGGATGAACGGGATGCACTCACCGAGATATTTAAATTAGTGCGTAAACATCAAAAGCAAAACCTCTG
TAACCGGTCAATTATCGTTGCGCATAACGCCACATTTGATCAAGGTTTTATTAATAAAGCCACTGAACGCTGCAACTTAA
AACGTATGCCATTCCATCCTTTTGTGAGCTTTGATACTGCCAGCATGGCCGGTTTGATTCTGGGACAAACGGTTCTTGCG
AAAGCCTGCCAACAGGCAAGTATTCCCTATGATAATAAGCAGGCACATTCGGCTTTATATGATACGGAAGTGACTGCAGA
TCTGTTTTTTTATATGGTAAATAAATTGAAGGCATTAGGAGGTTGGCCACTCGCGCCGGTTTAA

Upstream 100 bases:

>100_bases
GAATGTTTGTTTAATAACTTACTCTGCTCCGCACATTGAAACTCGCATTTACAGGCTGCATGGGTATAATCGCTTTTTTT
ATTGAGACCTAGAGTAAATT

Downstream 100 bases:

>100_bases
GCGGGGGGTAGGGTATGACTTTTCAGTTTAATATTGTGCTGACGTCGCGTTTATTCTTAGACAATAAAAAAGGGATACAT
TCAGTATCCCTTTTTTCTGG

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MENLLDKRFRGFFPVIIDIETSGLNAQTDAILEIAAILPAMDSQGYLFAAEKIHFNVLPFEGANLDPKALAFTGIDPFNP
LRAAVDERDALTEIFKLVRKHQKQNLCNRSIIVAHNATFDQGFINKATERCNLKRMPFHPFVSFDTASMAGLILGQTVLA
KACQQASIPYDNKQAHSALYDTEVTADLFFYMVNKLKALGGWPLAPV

Sequences:

>Translated_207_residues
MENLLDKRFRGFFPVIIDIETSGLNAQTDAILEIAAILPAMDSQGYLFAAEKIHFNVLPFEGANLDPKALAFTGIDPFNP
LRAAVDERDALTEIFKLVRKHQKQNLCNRSIIVAHNATFDQGFINKATERCNLKRMPFHPFVSFDTASMAGLILGQTVLA
KACQQASIPYDNKQAHSALYDTEVTADLFFYMVNKLKALGGWPLAPV
>Mature_207_residues
MENLLDKRFRGFFPVIIDIETSGLNAQTDAILEIAAILPAMDSQGYLFAAEKIHFNVLPFEGANLDPKALAFTGIDPFNP
LRAAVDERDALTEIFKLVRKHQKQNLCNRSIIVAHNATFDQGFINKATERCNLKRMPFHPFVSFDTASMAGLILGQTVLA
KACQQASIPYDNKQAHSALYDTEVTADLFFYMVNKLKALGGWPLAPV

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=201, Percent_Identity=63.18407960199, Blast_Score=263, Evalue=6e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 23004; Mature: 23004

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLLDKRFRGFFPVIIDIETSGLNAQTDAILEIAAILPAMDSQGYLFAAEKIHFNVLPF
CCHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEECC
EGANLDPKALAFTGIDPFNPLRAAVDERDALTEIFKLVRKHQKQNLCNRSIIVAHNATFD
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
QGFINKATERCNLKRMPFHPFVSFDTASMAGLILGQTVLAKACQQASIPYDNKQAHSALY
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
DTEVTADLFFYMVNKLKALGGWPLAPV
HHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MENLLDKRFRGFFPVIIDIETSGLNAQTDAILEIAAILPAMDSQGYLFAAEKIHFNVLPF
CCHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEECC
EGANLDPKALAFTGIDPFNPLRAAVDERDALTEIFKLVRKHQKQNLCNRSIIVAHNATFD
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
QGFINKATERCNLKRMPFHPFVSFDTASMAGLILGQTVLAKACQQASIPYDNKQAHSALY
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
DTEVTADLFFYMVNKLKALGGWPLAPV
HHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA