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The map label for this gene is dapE

Identifier: 119946093

GI number: 119946093

Start: 2991148

End: 2992281

Strand: Direct

Name: dapE

Synonym: Ping_2443

Alternate gene names: 119946093

Gene position: 2991148-2992281 (Clockwise)

Preceding gene: 119946092

Following gene: 119946094

Centisome position: 65.6

GC content: 44.62

Gene sequence:

>1134_bases
ATGTCAGACAGCCCAGTTTTGGCTCTTTTAAAAGACTTAATAAGCCGACGTTCGATTACCCCAAGCGACGAAGGGTGCCA
GCAGTTATTAATATCTAGACTTGAAAAATTAGGCTTTGAGTGTGAAGTAATGATCTTTGCAGATACTACCAATTTATGGG
CACGCAGAGGCACAGAAAAACCGCTTTTTTGTTTTGCGGGACACACCGATGTGGTACCAACCGGCCCTGAAGAAAATTGG
ACTTACCCTCCCTTTGAGCCAACCGTTGTCGATGGTTTTTTATTTGGCCGTGGCGCTGCGGATATGAAAAGTGGTATTGC
CGCTTTTATCATTGCGTTAGAGCAGTTCATCAAAGCACACCAGGATCACCAGGGTTCCATTGCCTTACTTATCACCAGCG
ATGAAGAGGGACCTTTTATCAACGGCACAGTGCGCGTAATTGAAACACTTGAAGCGCGCAATGAAAAAATAGATTATTGT
ATTGTTGGAGAGCCCTCAAGCACAGAGCATGTGGGTGATACTATTAAAAATGGTCGCAGAGGGTCATTAACGGCCGATTT
AACCATTAATGGCGTACAGGGTCACGTGGCTTACCCGCACATGGTCGAAAACCCTATTCATACCTGCATCGGACCGCTCA
CTGAGTTATCACAGATGGAGTGGGATAAGGGTAATAAGTATTTCCCACCAACCAGTTTTCAGGTAACCAATTTTAATGCT
GGCACGGGCGCGAGTAATGTTGTTCCTGGAGAAGCACAGATTCAGTTTAATTTTCGTTATTCGACCGAGTTAACAGCCGA
GATGATAATTGCACGTGTGGTAGCTATTTTGCAAAAACATAAGCTTAATTACCAGATTAAGTGGACTTATAACGGCTCAC
CATTTATTACCCAACCAGGTTCGTTGACTAATGCCGTAAGTGAGGCTATCTTTGCTGTCAATGGATTAACAACTGAGCTT
TCCACCAGTGGTGGGACCTCCGATGCACGTTTTATTGCACCGACAGGAGCGCAAGTTGTTGAACTGGGTCCCGGCAATAA
AACCATTCACAAAGTGAATGAATCAGTAAAAATTGCCGACCTGGAGGAGCTGGTACATATTCACCGGCTGACTCTGGAAA
AATTGTTAACATGA

Upstream 100 bases:

>100_bases
ACATTAATCAAGCGCCCTCTTCTGCGCCATAATAATCACTATCAGCTAGGTTTTAACATCGATACTTACCAAACTCTATT
TTCATTATAAAGGAAACATT

Downstream 100 bases:

>100_bases
TAGCTGAGCAATTAACCGGTCAAGTGTATTCACATTTGACCCAACTTTCAGATGATCTTTATGTGCATAAACAGGTTGTC
TCTGCTTTTAAGGGACTGCA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSDSPVLALLKDLISRRSITPSDEGCQQLLISRLEKLGFECEVMIFADTTNLWARRGTEKPLFCFAGHTDVVPTGPEENW
TYPPFEPTVVDGFLFGRGAADMKSGIAAFIIALEQFIKAHQDHQGSIALLITSDEEGPFINGTVRVIETLEARNEKIDYC
IVGEPSSTEHVGDTIKNGRRGSLTADLTINGVQGHVAYPHMVENPIHTCIGPLTELSQMEWDKGNKYFPPTSFQVTNFNA
GTGASNVVPGEAQIQFNFRYSTELTAEMIIARVVAILQKHKLNYQIKWTYNGSPFITQPGSLTNAVSEAIFAVNGLTTEL
STSGGTSDARFIAPTGAQVVELGPGNKTIHKVNESVKIADLEELVHIHRLTLEKLLT

Sequences:

>Translated_377_residues
MSDSPVLALLKDLISRRSITPSDEGCQQLLISRLEKLGFECEVMIFADTTNLWARRGTEKPLFCFAGHTDVVPTGPEENW
TYPPFEPTVVDGFLFGRGAADMKSGIAAFIIALEQFIKAHQDHQGSIALLITSDEEGPFINGTVRVIETLEARNEKIDYC
IVGEPSSTEHVGDTIKNGRRGSLTADLTINGVQGHVAYPHMVENPIHTCIGPLTELSQMEWDKGNKYFPPTSFQVTNFNA
GTGASNVVPGEAQIQFNFRYSTELTAEMIIARVVAILQKHKLNYQIKWTYNGSPFITQPGSLTNAVSEAIFAVNGLTTEL
STSGGTSDARFIAPTGAQVVELGPGNKTIHKVNESVKIADLEELVHIHRLTLEKLLT
>Mature_376_residues
SDSPVLALLKDLISRRSITPSDEGCQQLLISRLEKLGFECEVMIFADTTNLWARRGTEKPLFCFAGHTDVVPTGPEENWT
YPPFEPTVVDGFLFGRGAADMKSGIAAFIIALEQFIKAHQDHQGSIALLITSDEEGPFINGTVRVIETLEARNEKIDYCI
VGEPSSTEHVGDTIKNGRRGSLTADLTINGVQGHVAYPHMVENPIHTCIGPLTELSQMEWDKGNKYFPPTSFQVTNFNAG
TGASNVVPGEAQIQFNFRYSTELTAEMIIARVVAILQKHKLNYQIKWTYNGSPFITQPGSLTNAVSEAIFAVNGLTTELS
TSGGTSDARFIAPTGAQVVELGPGNKTIHKVNESVKIADLEELVHIHRLTLEKLLT

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=373, Percent_Identity=57.372654155496, Blast_Score=453, Evalue=1e-129,
Organism=Escherichia coli, GI1790395, Length=225, Percent_Identity=31.5555555555556, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_PSYIN (A1SXF9)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943773.1
- ProteinModelPortal:   A1SXF9
- SMR:   A1SXF9
- STRING:   A1SXF9
- MEROPS:   M20.010
- GeneID:   4626409
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_2443
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- PhylomeDB:   A1SXF9
- ProtClustDB:   PRK13009
- BioCyc:   PING357804:PING_2443-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41272; Mature: 41141

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 70-70 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSPVLALLKDLISRRSITPSDEGCQQLLISRLEKLGFECEVMIFADTTNLWARRGTEK
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHCCCCC
PLFCFAGHTDVVPTGPEENWTYPPFEPTVVDGFLFGRGAADMKSGIAAFIIALEQFIKAH
CEEEECCCCCCCCCCCCCCCCCCCCCCCEEEHEEECCCCHHHHHHHHHHHHHHHHHHHHH
QDHQGSIALLITSDEEGPFINGTVRVIETLEARNEKIDYCIVGEPSSTEHVGDTIKNGRR
CCCCCCEEEEEECCCCCCEECCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHCCCC
GSLTADLTINGVQGHVAYPHMVENPIHTCIGPLTELSQMEWDKGNKYFPPTSFQVTNFNA
CCEEEEEEEECCCCCEECCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCC
GTGASNVVPGEAQIQFNFRYSTELTAEMIIARVVAILQKHKLNYQIKWTYNGSPFITQPG
CCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEECCC
SLTNAVSEAIFAVNGLTTELSTSGGTSDARFIAPTGAQVVELGPGNKTIHKVNESVKIAD
CHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECCCCEEEEECCCCCHHHHHCCCEEEHH
LEELVHIHRLTLEKLLT
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDSPVLALLKDLISRRSITPSDEGCQQLLISRLEKLGFECEVMIFADTTNLWARRGTEK
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHCCCCC
PLFCFAGHTDVVPTGPEENWTYPPFEPTVVDGFLFGRGAADMKSGIAAFIIALEQFIKAH
CEEEECCCCCCCCCCCCCCCCCCCCCCCEEEHEEECCCCHHHHHHHHHHHHHHHHHHHHH
QDHQGSIALLITSDEEGPFINGTVRVIETLEARNEKIDYCIVGEPSSTEHVGDTIKNGRR
CCCCCCEEEEEECCCCCCEECCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHCCCC
GSLTADLTINGVQGHVAYPHMVENPIHTCIGPLTELSQMEWDKGNKYFPPTSFQVTNFNA
CCEEEEEEEECCCCCEECCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCC
GTGASNVVPGEAQIQFNFRYSTELTAEMIIARVVAILQKHKLNYQIKWTYNGSPFITQPG
CCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEECCC
SLTNAVSEAIFAVNGLTTELSTSGGTSDARFIAPTGAQVVELGPGNKTIHKVNESVKIAD
CHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECCCCEEEEECCCCCHHHHHCCCEEEHH
LEELVHIHRLTLEKLLT
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA