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The map label for this gene is cynR [H]

Identifier: 119946067

GI number: 119946067

Start: 2961235

End: 2962128

Strand: Reverse

Name: cynR [H]

Synonym: Ping_2416

Alternate gene names: 119946067

Gene position: 2962128-2961235 (Counterclockwise)

Preceding gene: 119946073

Following gene: 119946066

Centisome position: 64.96

GC content: 42.84

Gene sequence:

>894_bases
ATGAAGCAACTTATAGATTTTCAGTATTTTCAGGCGGTCGCTAAGGCAGGTTCAATTCGTAAGGCTGCCGACAAACTCTC
GATCACTTCAACAGCACTTAACCGACGTATTCTTACGATGGAAGATGAACTGGGTGAAAAACTATTTGAGCGTCAAGCCA
ATGGTGTACGCCTGTCTGCTGCAGGAGAGTTATTCTTACATCACGTCCAAAAACAACTTGCTGATCTTCAAAGAGTTAAA
AGTCAAATATCAGATCTTAGCGGGGTCAGGCGAGGGCATGTCAATATCGCATGCAGTCAAGCACTGCTGACGTCATTTTT
TCCGCAACAAATTGCACGTTTTCGTCACCAACACCCCGCGGTAACTTTTAGTCTTTATGTAAGAGATAGGCAGGCGGCAG
AATCGACATTATTAGACAATAGTGTCGATTTAGCCATGGTATTTGAACCCGTGCACATGAATGAACTGCAGATTCTTATA
TCTAAAGAACAACAGCTGTACGCCATTATGCCAAGTGGCCATCCATTAGCTGAAAAAAGCGACGTTAAATTAAGTGAATG
TATGGCTTATCCCTTAGCACTTCCCACAAGCGAGTACGGCGTACGTCATATTTTGGATTTTAAAGCCAATAGAACCATGC
TTAATATGCAACCCACTGTGGAATCCGACAGCTTTGAGTTTCTGCGTAATTATGTGCTTAACGAGGGTTGCATCACCTTT
CAAATAGAAATTGGATTACCGCAAATTATGGAAGATTTAGGGCTAATTGCGCGGCCGATATCAAAGGCCGATATGGCAGC
CGGCAGTATGTACCTTATTCAGAAAAAAAATCGTACATTACCCACGGCTGCTGCACGTTTTGCAGAACAGCTAATACAAA
CACTGTCACAGTAA

Upstream 100 bases:

>100_bases
TATACTGCTTGTTTTGCATTTGTGTGGTGCATATTTTGCATCGCATGGGTAAGGGGGTAATATATCAATTATCTAAATCT
TGGTTAATGGAAGAATACAA

Downstream 100 bases:

>100_bases
TAACAATCCAATAGTTAAAGGATCACAGTATAAAACCGTGGTTGTTGCCTATTATCGATAGGAAATCCTCACTGCCTTGC
ATAAATGCCGACGCGTTTTC

Product: transcriptional regulator, substrate-binding of LysR family protein

Products: NA

Alternate protein names: Cyn operon transcriptional activator [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MKQLIDFQYFQAVAKAGSIRKAADKLSITSTALNRRILTMEDELGEKLFERQANGVRLSAAGELFLHHVQKQLADLQRVK
SQISDLSGVRRGHVNIACSQALLTSFFPQQIARFRHQHPAVTFSLYVRDRQAAESTLLDNSVDLAMVFEPVHMNELQILI
SKEQQLYAIMPSGHPLAEKSDVKLSECMAYPLALPTSEYGVRHILDFKANRTMLNMQPTVESDSFEFLRNYVLNEGCITF
QIEIGLPQIMEDLGLIARPISKADMAAGSMYLIQKKNRTLPTAAARFAEQLIQTLSQ

Sequences:

>Translated_297_residues
MKQLIDFQYFQAVAKAGSIRKAADKLSITSTALNRRILTMEDELGEKLFERQANGVRLSAAGELFLHHVQKQLADLQRVK
SQISDLSGVRRGHVNIACSQALLTSFFPQQIARFRHQHPAVTFSLYVRDRQAAESTLLDNSVDLAMVFEPVHMNELQILI
SKEQQLYAIMPSGHPLAEKSDVKLSECMAYPLALPTSEYGVRHILDFKANRTMLNMQPTVESDSFEFLRNYVLNEGCITF
QIEIGLPQIMEDLGLIARPISKADMAAGSMYLIQKKNRTLPTAAARFAEQLIQTLSQ
>Mature_297_residues
MKQLIDFQYFQAVAKAGSIRKAADKLSITSTALNRRILTMEDELGEKLFERQANGVRLSAAGELFLHHVQKQLADLQRVK
SQISDLSGVRRGHVNIACSQALLTSFFPQQIARFRHQHPAVTFSLYVRDRQAAESTLLDNSVDLAMVFEPVHMNELQILI
SKEQQLYAIMPSGHPLAEKSDVKLSECMAYPLALPTSEYGVRHILDFKANRTMLNMQPTVESDSFEFLRNYVLNEGCITF
QIEIGLPQIMEDLGLIARPISKADMAAGSMYLIQKKNRTLPTAAARFAEQLIQTLSQ

Specific function: Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR- cynTSX intergenic region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=232, Percent_Identity=29.3103448275862, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI145693105, Length=204, Percent_Identity=25.9803921568627, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787601, Length=284, Percent_Identity=26.4084507042254, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787806, Length=188, Percent_Identity=27.1276595744681, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789204, Length=176, Percent_Identity=29.5454545454545, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788481, Length=201, Percent_Identity=27.363184079602, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI48994962, Length=168, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787879, Length=297, Percent_Identity=22.8956228956229, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1787530, Length=226, Percent_Identity=22.1238938053097, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788887, Length=180, Percent_Identity=25.5555555555556, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789506, Length=242, Percent_Identity=23.1404958677686, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33411; Mature: 33411

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLIDFQYFQAVAKAGSIRKAADKLSITSTALNRRILTMEDELGEKLFERQANGVRLSA
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHCCCCEEEEH
AGELFLHHVQKQLADLQRVKSQISDLSGVRRGHVNIACSQALLTSFFPQQIARFRHQHPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
VTFSLYVRDRQAAESTLLDNSVDLAMVFEPVHMNELQILISKEQQLYAIMPSGHPLAEKS
EEEEEEEECHHHHHHHHHCCCCCEEEEECCCCCCHHHEEEECCCEEEEECCCCCCCCCCC
DVKLSECMAYPLALPTSEYGVRHILDFKANRTMLNMQPTVESDSFEFLRNYVLNEGCITF
CCCHHHHHHCCCCCCCCHHCHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEE
QIEIGLPQIMEDLGLIARPISKADMAAGSMYLIQKKNRTLPTAAARFAEQLIQTLSQ
EEECCCHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQLIDFQYFQAVAKAGSIRKAADKLSITSTALNRRILTMEDELGEKLFERQANGVRLSA
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHCCCCEEEEH
AGELFLHHVQKQLADLQRVKSQISDLSGVRRGHVNIACSQALLTSFFPQQIARFRHQHPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
VTFSLYVRDRQAAESTLLDNSVDLAMVFEPVHMNELQILISKEQQLYAIMPSGHPLAEKS
EEEEEEEECHHHHHHHHHCCCCCEEEEECCCCCCHHHEEEECCCEEEEECCCCCCCCCCC
DVKLSECMAYPLALPTSEYGVRHILDFKANRTMLNMQPTVESDSFEFLRNYVLNEGCITF
CCCHHHHHHCCCCCCCCHHCHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEE
QIEIGLPQIMEDLGLIARPISKADMAAGSMYLIQKKNRTLPTAAARFAEQLIQTLSQ
EEECCCHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]