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The map label for this gene is thiD [H]

Identifier: 119946034

GI number: 119946034

Start: 2894400

End: 2895251

Strand: Reverse

Name: thiD [H]

Synonym: Ping_2378

Alternate gene names: 119946034

Gene position: 2895251-2894400 (Counterclockwise)

Preceding gene: 119946037

Following gene: 119946033

Centisome position: 63.5

GC content: 46.13

Gene sequence:

>852_bases
ATGTCAAACCTTAGTGCAACTTCTACTGCTCAAAATTCATCAACGCCTATCGTGCTGACTATTGCCGGATCCGACAGTGG
TGGTGGAGCAGGTATTCAAGCCGATATTAAAGCCATTTCAGCAACGGGCGGTTACGCCTGTTCTGTGATCACTGTGATTA
CCGCTCAAAACACCCTTGGGGTGTCCGCTATTTTCCCTATTCCCCTTGAACATATAGAAAAGCAACTTGATGCGGTTTTT
AGCGATCTGAATGTGGTTGCTGTTAAGGTTGGGATGTTGGCTGATTGCGACATAATCAAACTCGTGGCAGCAAAAATAAG
ACAGTATCGTCCTGCCTTTTTAGTGGTTGATCCGGTGATGATCGCAACCAGCGGAGACTTATTGCTGAAAGCATATTCGA
TTAGTACACTGAAATCGGAACTGCTTCCTCTTGCAGATCTTATCACGCCTAATCTGCCCGAAGCGGCGGAATTGATTTCT
TCAGATGTGCCAACCAATAAAGAGGGCATGGAGAAGATGATCGGCAAACTCCGTAAATTGAAATTTAATGCGCTGCTGCT
TAAAGGGGGGCATTTGATTGATGATGAAAATAGTAATGATCTGCTTATCCTACCTAATCATGTTGAACAGTTAAACGCCA
TCCGTATCAATACGCTTAATACCCATGGTACAGGTTGCACACTTTCGTCGGCAATCGCCTCCTATCTGGCGCAGGGTAAT
GATCTTTTACGTGCGGTGCAGCTGGGCAAAGACTATATTTCGCAAGCGATCGCGCATGCCGACGAATTAGATGTCGGGCA
GGGTCATGGCCCGGTAAACCATTTCTTTGTCGGACATGCGAATGTCCGTTAG

Upstream 100 bases:

>100_bases
TGGCGTAGGGAACTATAAGTCTTGTTTTTGATTCTCTCTGCTACACCTATCTTAAATATTTGGCTTGATATAGTTCCTTT
TACCCGTTATAGGAGCTTTT

Downstream 100 bases:

>100_bases
CACGCGGCCTTTATCAGTTAAAAGTCCTGCCCTGCAGATAGATATCTCTGCGGGTTATCTGCGTTATACCGGCAGTAAAG
CGGCCACATTACAGGGTATT

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSNLSATSTAQNSSTPIVLTIAGSDSGGGAGIQADIKAISATGGYACSVITVITAQNTLGVSAIFPIPLEHIEKQLDAVF
SDLNVVAVKVGMLADCDIIKLVAAKIRQYRPAFLVVDPVMIATSGDLLLKAYSISTLKSELLPLADLITPNLPEAAELIS
SDVPTNKEGMEKMIGKLRKLKFNALLLKGGHLIDDENSNDLLILPNHVEQLNAIRINTLNTHGTGCTLSSAIASYLAQGN
DLLRAVQLGKDYISQAIAHADELDVGQGHGPVNHFFVGHANVR

Sequences:

>Translated_283_residues
MSNLSATSTAQNSSTPIVLTIAGSDSGGGAGIQADIKAISATGGYACSVITVITAQNTLGVSAIFPIPLEHIEKQLDAVF
SDLNVVAVKVGMLADCDIIKLVAAKIRQYRPAFLVVDPVMIATSGDLLLKAYSISTLKSELLPLADLITPNLPEAAELIS
SDVPTNKEGMEKMIGKLRKLKFNALLLKGGHLIDDENSNDLLILPNHVEQLNAIRINTLNTHGTGCTLSSAIASYLAQGN
DLLRAVQLGKDYISQAIAHADELDVGQGHGPVNHFFVGHANVR
>Mature_282_residues
SNLSATSTAQNSSTPIVLTIAGSDSGGGAGIQADIKAISATGGYACSVITVITAQNTLGVSAIFPIPLEHIEKQLDAVFS
DLNVVAVKVGMLADCDIIKLVAAKIRQYRPAFLVVDPVMIATSGDLLLKAYSISTLKSELLPLADLITPNLPEAAELISS
DVPTNKEGMEKMIGKLRKLKFNALLLKGGHLIDDENSNDLLILPNHVEQLNAIRINTLNTHGTGCTLSSAIASYLAQGND
LLRAVQLGKDYISQAIAHADELDVGQGHGPVNHFFVGHANVR

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=257, Percent_Identity=47.8599221789883, Blast_Score=238, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6325378, Length=281, Percent_Identity=35.9430604982206, Blast_Score=138, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6324997, Length=279, Percent_Identity=36.9175627240143, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324517, Length=283, Percent_Identity=36.3957597173145, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 29812; Mature: 29680

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLSATSTAQNSSTPIVLTIAGSDSGGGAGIQADIKAISATGGYACSVITVITAQNTLG
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEEEECCCCCEEEEEEEEEECCCCC
VSAIFPIPLEHIEKQLDAVFSDLNVVAVKVGMLADCDIIKLVAAKIRQYRPAFLVVDPVM
EEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCEE
IATSGDLLLKAYSISTLKSELLPLADLITPNLPEAAELISSDVPTNKEGMEKMIGKLRKL
EEECCCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
KFNALLLKGGHLIDDENSNDLLILPNHVEQLNAIRINTLNTHGTGCTLSSAIASYLAQGN
HEEEEEEECCEEECCCCCCCEEEECCHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHCCH
DLLRAVQLGKDYISQAIAHADELDVGQGHGPVNHFFVGHANVR
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCC
>Mature Secondary Structure 
SNLSATSTAQNSSTPIVLTIAGSDSGGGAGIQADIKAISATGGYACSVITVITAQNTLG
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEEEECCCCCEEEEEEEEEECCCCC
VSAIFPIPLEHIEKQLDAVFSDLNVVAVKVGMLADCDIIKLVAAKIRQYRPAFLVVDPVM
EEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCEE
IATSGDLLLKAYSISTLKSELLPLADLITPNLPEAAELISSDVPTNKEGMEKMIGKLRKL
EEECCCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
KFNALLLKGGHLIDDENSNDLLILPNHVEQLNAIRINTLNTHGTGCTLSSAIASYLAQGN
HEEEEEEECCEEECCCCCCCEEEECCHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHCCH
DLLRAVQLGKDYISQAIAHADELDVGQGHGPVNHFFVGHANVR
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]