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The map label for this gene is glgX [H]

Identifier: 119946019

GI number: 119946019

Start: 2874523

End: 2876628

Strand: Reverse

Name: glgX [H]

Synonym: Ping_2363

Alternate gene names: 119946019

Gene position: 2876628-2874523 (Counterclockwise)

Preceding gene: 119946020

Following gene: 119946018

Centisome position: 63.09

GC content: 40.03

Gene sequence:

>2106_bases
TTGGAAGATAAACTATTAGAACTGGATAAAAAGATGCATCCTTTCTCTGCATCTCGCGGGCAGTTTCTGCCCCTTGGTGC
AATTCTAGACCAAGGAGGCTGTCACTTTTCAGTTTATAGCTTAGGCGCTTACCGGGTTGACTTATGTTTATTTGATAAAC
ATGAAAATGAAATTGCTAACTATCCTCTTGAAGTCAAACAGGGGCGGATGTGGAGCATTTTTGTCGATCAGGTCATAAAA
GGACAGCTTTACGGGTATCGGGTATACGGTGATTACCAACCAGAAAATGGTCTTTTATTTAATCCAAATAATTTATTAAT
TGATCCCTATGCAAGGGCTTTAAATCGTGTACAGCATAACTTTGCTCAGGCACAGAGTGATGACGAGCACTCACAAGTGG
CTAAGTCAGTTGTTATTGATAATAGTTTTGACTGGAAAGATGTCGCTAAACCCAATATTGCTGAGGATGAACGCATCATT
TACGAAGTACATGTAAAGAGCTTCAGCCAGCGTAATAAAAAAATTCCTTTGGAAAAACGTGGTAAATATCTTGGTTTATC
ACATCCTGAAAGTATTAAACATTATAAGGCCCTTGGCATTACCAGTTTACAGATAATGCCCGTCTTTAGCTTTGTGAGTG
AATTAAGGCTTAAAAATATTGGTTTGACGAATTACTGGGGTTATAACCCCATTAATTTCTTTGCGCCCGAAAATCGTTAC
GCACAATTTGATGCCGTTAATGAATTTAAAACCATGGTGCGCGAACTTCACGCTGAAGGGATAGAGATTATTTTAGATGT
GGTTTATAACCATAGCGCAGAGGCAGGAAATAATGGCCTGATTCTTAGTTTAAAAGGGTTTGATAACCGTAATTATTATA
CCTTTGAAAAAGAAGTTGATGGCAGCCATCTTAATTATTCAAATTATAGCAATCACTCCGGCTGTGGTAATACCTTAAAC
CTTGATTCCCCTTGGTCTTTAAAGTTAATGCTTGATTCATTGCGTTATTGGGCAACAGAAATGCAGGTTGATGGTTTCCG
TTTTGACCTGGCTGTTACACTTGCCCGTGAAGCGAATGGTTTTAATAAACACAGTGCCTTTTTTAAAGCACTGTTGCAGG
ATCCTGTTTTAAGTCGCGTCAAATTGATTGCTGAGCCTTGGGATATAGGGCCTGGAGGATATCGTTTAGGTGGATTTCCC
TGTGATTGGTTAGAGTGTAATGACCGTTACCGCGATACACTACGTTCTTTCTGGCGTGGAGATACCGGCAATGTTGGCGA
TCTTGCAACGCGTTTATTGGGTTCCCGTGACGTTTTTGCCAAGAGTTACCGCGCTATTTCCAGTAGCGTCAATTACATTT
GCTATCACGATGGTTTTACAATGGATGATCTTGTTTCCTATGATCGCAGGCATAATGAAGCTAATGGTGAAAAAAGCCGC
GATGGTCATGGCCATAATTTATCTTCTAATTATGGTGTTGAAGGCCAAACCGATGATGTCAAAATCAATCAAATACGCCA
ACAGCAAAAACGTAATTTAATTGCCAGTTTATTTATTTCCCAGGGCACCCCCCATTTTTTATCGGGCGATGAAATGGGTC
ATACCCAAACCGGTAATAATAATGCTTATTGTCAGGATAATATCTTAACTTGGTTAAGCTGGAATTTGACTGATGCTGAT
AAAAAACTCTTTGCTTTTACCAGTGAGGTTATCAAGCTGCGTAAAGAATCTCGTTTATTTGGTAGTCTTTCACTCAGTGG
TGACAGTACTGGCCGCTGTGAAAATTCAGATCTTGTTCAGTGGTATCACCCTAATGGTGAGGTAATGGAAGAGGTTGACT
GGCACGCGAGTAATTCACAAGCGATAGCGGTTGAATTGCGCGAGGCCGAACAATCGGGTGAGCGTTGGTTTGTAGTATTA
AACTCGTCCAGTTATCACATTACTTGTAACCTACCCTTGTTAGAGGTGGGTTTATCTTGGAAAATAAAAATTAATACGGT
GTGTTCGTCGGGGAAATTAACTGACGAAGAGACTAATATTACCAAAGTGACTCAAGTTAAAGCCAGATCAGTTATCTTGT
TAAAGTGTGTACGGCACTTAACTTAA

Upstream 100 bases:

>100_bases
CTTTGTTGCAGAAGAACAACAGTGGCAAGGCAAGGAATATTCAATTAAACTGAATTTACCCCCTTTATCTACTGTGTATT
TACAAAAAAAGGTAAAATAA

Downstream 100 bases:

>100_bases
TGATTAAGGCGACAAACATAAATTGCAGGTAATAATTTATGTTAAAAACTAAATTTAAAGCCCTGGCAATCTCTTTGTTA
GGGCTTTTTTATGGGATAAT

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 701; Mature: 701

Protein sequence:

>701_residues
MEDKLLELDKKMHPFSASRGQFLPLGAILDQGGCHFSVYSLGAYRVDLCLFDKHENEIANYPLEVKQGRMWSIFVDQVIK
GQLYGYRVYGDYQPENGLLFNPNNLLIDPYARALNRVQHNFAQAQSDDEHSQVAKSVVIDNSFDWKDVAKPNIAEDERII
YEVHVKSFSQRNKKIPLEKRGKYLGLSHPESIKHYKALGITSLQIMPVFSFVSELRLKNIGLTNYWGYNPINFFAPENRY
AQFDAVNEFKTMVRELHAEGIEIILDVVYNHSAEAGNNGLILSLKGFDNRNYYTFEKEVDGSHLNYSNYSNHSGCGNTLN
LDSPWSLKLMLDSLRYWATEMQVDGFRFDLAVTLAREANGFNKHSAFFKALLQDPVLSRVKLIAEPWDIGPGGYRLGGFP
CDWLECNDRYRDTLRSFWRGDTGNVGDLATRLLGSRDVFAKSYRAISSSVNYICYHDGFTMDDLVSYDRRHNEANGEKSR
DGHGHNLSSNYGVEGQTDDVKINQIRQQQKRNLIASLFISQGTPHFLSGDEMGHTQTGNNNAYCQDNILTWLSWNLTDAD
KKLFAFTSEVIKLRKESRLFGSLSLSGDSTGRCENSDLVQWYHPNGEVMEEVDWHASNSQAIAVELREAEQSGERWFVVL
NSSSYHITCNLPLLEVGLSWKIKINTVCSSGKLTDEETNITKVTQVKARSVILLKCVRHLT

Sequences:

>Translated_701_residues
MEDKLLELDKKMHPFSASRGQFLPLGAILDQGGCHFSVYSLGAYRVDLCLFDKHENEIANYPLEVKQGRMWSIFVDQVIK
GQLYGYRVYGDYQPENGLLFNPNNLLIDPYARALNRVQHNFAQAQSDDEHSQVAKSVVIDNSFDWKDVAKPNIAEDERII
YEVHVKSFSQRNKKIPLEKRGKYLGLSHPESIKHYKALGITSLQIMPVFSFVSELRLKNIGLTNYWGYNPINFFAPENRY
AQFDAVNEFKTMVRELHAEGIEIILDVVYNHSAEAGNNGLILSLKGFDNRNYYTFEKEVDGSHLNYSNYSNHSGCGNTLN
LDSPWSLKLMLDSLRYWATEMQVDGFRFDLAVTLAREANGFNKHSAFFKALLQDPVLSRVKLIAEPWDIGPGGYRLGGFP
CDWLECNDRYRDTLRSFWRGDTGNVGDLATRLLGSRDVFAKSYRAISSSVNYICYHDGFTMDDLVSYDRRHNEANGEKSR
DGHGHNLSSNYGVEGQTDDVKINQIRQQQKRNLIASLFISQGTPHFLSGDEMGHTQTGNNNAYCQDNILTWLSWNLTDAD
KKLFAFTSEVIKLRKESRLFGSLSLSGDSTGRCENSDLVQWYHPNGEVMEEVDWHASNSQAIAVELREAEQSGERWFVVL
NSSSYHITCNLPLLEVGLSWKIKINTVCSSGKLTDEETNITKVTQVKARSVILLKCVRHLT
>Mature_701_residues
MEDKLLELDKKMHPFSASRGQFLPLGAILDQGGCHFSVYSLGAYRVDLCLFDKHENEIANYPLEVKQGRMWSIFVDQVIK
GQLYGYRVYGDYQPENGLLFNPNNLLIDPYARALNRVQHNFAQAQSDDEHSQVAKSVVIDNSFDWKDVAKPNIAEDERII
YEVHVKSFSQRNKKIPLEKRGKYLGLSHPESIKHYKALGITSLQIMPVFSFVSELRLKNIGLTNYWGYNPINFFAPENRY
AQFDAVNEFKTMVRELHAEGIEIILDVVYNHSAEAGNNGLILSLKGFDNRNYYTFEKEVDGSHLNYSNYSNHSGCGNTLN
LDSPWSLKLMLDSLRYWATEMQVDGFRFDLAVTLAREANGFNKHSAFFKALLQDPVLSRVKLIAEPWDIGPGGYRLGGFP
CDWLECNDRYRDTLRSFWRGDTGNVGDLATRLLGSRDVFAKSYRAISSSVNYICYHDGFTMDDLVSYDRRHNEANGEKSR
DGHGHNLSSNYGVEGQTDDVKINQIRQQQKRNLIASLFISQGTPHFLSGDEMGHTQTGNNNAYCQDNILTWLSWNLTDAD
KKLFAFTSEVIKLRKESRLFGSLSLSGDSTGRCENSDLVQWYHPNGEVMEEVDWHASNSQAIAVELREAEQSGERWFVVL
NSSSYHITCNLPLLEVGLSWKIKINTVCSSGKLTDEETNITKVTQVKARSVILLKCVRHLT

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=567, Percent_Identity=44.2680776014109, Blast_Score=471, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 79793; Mature: 79793

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDKLLELDKKMHPFSASRGQFLPLGAILDQGGCHFSVYSLGAYRVDLCLFDKHENEIAN
CCCHHHHHHHHCCCCCCCCCCEEEHHHHHCCCCCEEEEEECCEEEEEEEEEECCCCHHHC
YPLEVKQGRMWSIFVDQVIKGQLYGYRVYGDYQPENGLLFNPNNLLIDPYARALNRVQHN
CCEEECCCCEEEHHHHHHHCCCEEEEEEEECCCCCCCEEECCCCEEECHHHHHHHHHHHH
FAQAQSDDEHSQVAKSVVIDNSFDWKDVAKPNIAEDERIIYEVHVKSFSQRNKKIPLEKR
HHHCCCCCHHHHHHHHHEECCCCCHHHCCCCCCCCCCEEEEEEEHHHHHHCCCCCCHHHC
GKYLGLSHPESIKHYKALGITSLQIMPVFSFVSELRLKNIGLTNYWGYNPINFFAPENRY
CCEECCCCCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCC
AQFDAVNEFKTMVRELHAEGIEIILDVVYNHSAEAGNNGLILSLKGFDNRNYYTFEKEVD
EEHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEEEECCC
GSHLNYSNYSNHSGCGNTLNLDSPWSLKLMLDSLRYWATEMQVDGFRFDLAVTLAREANG
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCEEEEEEEEEEHHCCC
FNKHSAFFKALLQDPVLSRVKLIAEPWDIGPGGYRLGGFPCDWLECNDRYRDTLRSFWRG
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCHHCCCHHHHHHHHHHHCC
DTGNVGDLATRLLGSRDVFAKSYRAISSSVNYICYHDGFTMDDLVSYDRRHNEANGEKSR
CCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCC
DGHGHNLSSNYGVEGQTDDVKINQIRQQQKRNLIASLFISQGTPHFLSGDEMGHTQTGNN
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
NAYCQDNILTWLSWNLTDADKKLFAFTSEVIKLRKESRLFGSLSLSGDSTGRCENSDLVQ
CCEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCEEE
WYHPNGEVMEEVDWHASNSQAIAVELREAEQSGERWFVVLNSSSYHITCNLPLLEVGLSW
EECCCCHHHHHHCCCCCCCCEEEEEEEHHHCCCCEEEEEEECCCEEEEECCCCEEECCEE
KIKINTVCSSGKLTDEETNITKVTQVKARSVILLKCVRHLT
EEEEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEDKLLELDKKMHPFSASRGQFLPLGAILDQGGCHFSVYSLGAYRVDLCLFDKHENEIAN
CCCHHHHHHHHCCCCCCCCCCEEEHHHHHCCCCCEEEEEECCEEEEEEEEEECCCCHHHC
YPLEVKQGRMWSIFVDQVIKGQLYGYRVYGDYQPENGLLFNPNNLLIDPYARALNRVQHN
CCEEECCCCEEEHHHHHHHCCCEEEEEEEECCCCCCCEEECCCCEEECHHHHHHHHHHHH
FAQAQSDDEHSQVAKSVVIDNSFDWKDVAKPNIAEDERIIYEVHVKSFSQRNKKIPLEKR
HHHCCCCCHHHHHHHHHEECCCCCHHHCCCCCCCCCCEEEEEEEHHHHHHCCCCCCHHHC
GKYLGLSHPESIKHYKALGITSLQIMPVFSFVSELRLKNIGLTNYWGYNPINFFAPENRY
CCEECCCCCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCC
AQFDAVNEFKTMVRELHAEGIEIILDVVYNHSAEAGNNGLILSLKGFDNRNYYTFEKEVD
EEHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEEEECCC
GSHLNYSNYSNHSGCGNTLNLDSPWSLKLMLDSLRYWATEMQVDGFRFDLAVTLAREANG
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCEEEEEEEEEEHHCCC
FNKHSAFFKALLQDPVLSRVKLIAEPWDIGPGGYRLGGFPCDWLECNDRYRDTLRSFWRG
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCHHCCCHHHHHHHHHHHCC
DTGNVGDLATRLLGSRDVFAKSYRAISSSVNYICYHDGFTMDDLVSYDRRHNEANGEKSR
CCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCC
DGHGHNLSSNYGVEGQTDDVKINQIRQQQKRNLIASLFISQGTPHFLSGDEMGHTQTGNN
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
NAYCQDNILTWLSWNLTDADKKLFAFTSEVIKLRKESRLFGSLSLSGDSTGRCENSDLVQ
CCEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCEEE
WYHPNGEVMEEVDWHASNSQAIAVELREAEQSGERWFVVLNSSSYHITCNLPLLEVGLSW
EECCCCHHHHHHCCCCCCCCEEEEEEEHHHCCCCEEEEEEECCCEEEEECCCCEEECCEE
KIKINTVCSSGKLTDEETNITKVTQVKARSVILLKCVRHLT
EEEEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]