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The map label for this gene is ligA

Identifier: 119945995

GI number: 119945995

Start: 2840668

End: 2842617

Strand: Reverse

Name: ligA

Synonym: Ping_2336

Alternate gene names: 119945995

Gene position: 2842617-2840668 (Counterclockwise)

Preceding gene: 119945996

Following gene: 119945994

Centisome position: 62.34

GC content: 38.92

Gene sequence:

>1950_bases
ATGATCACATTAAGTAAAAGTCAGGAAGAAGTATTTAAAAAACTAGCTGTGGAGTTATCAATGTCTAAGCTAATTGAAGC
CTGTAATAAAAGTGTTGATTTTTCATCCCTTAATTCAAATGAACAGCTTGATGTTTTAAAGGTAACCAATGCGTTTTACC
GTTCAGCTGAGCAAATCATTAATGATTCAACTTACGATGCTTTCCTAAGTGAGTTCAGTACTTATAATCCCGATCATCCT
TACTTATTAACTGTTGAGCCTGAAGTATTAGCGGATAGCAAAACAGTTCCTTTGCCAAAAAAAATGCTATCGACAGATAA
AGCATATTCTTTTGAAGAAATTAAAAAATGGATTGATAGATTGTTAAAAGCCGCGATAGAAGTCGGTGTAAGCGAATCTG
AAATACAAATCAAAGTCACTCCAAAGCTAGATGGCTATGCTGCATATGATGATGGGATATCTCTTTATACTCGTGGAGAC
GGTGCCAGAGGCCAAGATATTACCCGAGCGTTTAATAAGGGTCTACAAGTTGCAAACAATGGGGATCGAGGCTTAGGGCC
GGGTGAAATTGTCATTAAGAAAAGCTATTTCGATACGGTTCTTAGTGATAAATTTGAAAATTCAAGAAATATTCAGGCAG
CCATTATTGCAGAAAAGAAGGTTGATGAATCCATTCAAAAGGCAATAGATGTAGGGGCTTGTGTATTTTTTCCTTTCTTA
TCACTTGAAAACTGGATTGGCCATTACACCGAAATTTTAGTCGATTTCGAATCAATTGTTGAAAAGATGTGGTCTGCTGT
AGATTATGATATTGATGGGGTTGTTTTAGAAGTAACAAATGAAACTCTAAAAGAGCATATGGGGGCGACACGTCACCATC
ATAGATGGCAGATTGCTTTTAAAGTAAATGCGGAATCAGCTGAAGTCAAAGTGTTAAATGTAACGCCGCAAACGTCAAGA
ACTGGACGTGTTACACCCGTCGCCGAGTTAGAGCCTACCAAGCTAAGTGGCGCAACAATTAGTAGAGCTACTGTACACCA
TTACAATATGGTTAAAACTAATGGTGTTGGCCCTGGTGCAATCGTGCAGCTTGTTAGAAGCGGACTCGTTATTCCAAAAA
TTGAGAAAGTGATTAAAGCGGTTGAGCCCCAGTTAGCCAAGGAATGCCCTAGTTGTGGTACGCATTTAATTTGGGAATCA
GACCATTTGGTATGCCCTAATAAAACCGACTGTCCAGCACAAACAGAGAATACCCTGGTCCATTTTTTCAAAACATTAGG
CAATAATGATGGTTTTGGTCCTAAGGTGATTGAAAAGCTTCATGAGTTTGGAATAAAGAAAATCCATGAAATCTATGAGC
TAAAACAAGAAAGCTTTGTCAGTTTTGGATTTGGCGATAAAACAGCTCAAAATTTAGTGGAGCAGCTTCAAGTTAGTCGT
GATGTTGAAATTGAAGACTGGCGATTTTTGGCCGCCTTTGGTGTAAGTCGATTAGCTGGGGGAAATTGTGAAAAATTATT
GCAACACCATTCATTAGACTCTTTGTTTGAAGCTACTGTGGAAGATTTAGTGCAGCTTGATGGTTTTGCACAAGTGAGTG
CAGATGCCATTGTAGAAGGCTTAGCCAATATAAAGGAAGAATTTCTTAAACTGAGTGCATTGAATTTTAATTTGACAATA
ACCCCGAAGGCTTCAGAAAAATCTCCGGGTGAAACCCCCGTCTTTGGAAAAATTATTGTGTTTACTGGCGCGATGACTCA
AGGTTCAAGAGGAGATATGGAGAAGCAAGCTAAAGCACTGGGCGCTAAGGTTGCTAAATCAGTAACCGGTAAGACTTCAC
TCCTTGTCACTGGCGATAAGGTAGGGGCAAATAAAATTAATGCCGCTCGTGATAAAGGAGTTCAGGTACTTTCAGAATTG
GATTACTTGGCGTTAATATCAACTTACTAG

Upstream 100 bases:

>100_bases
ATTGAGGATTAGCTAAAAGAATAGAAGCGAAGTTTACGAATGAGCATGAACAGATATAATAGCTAAATATAATAGCGTTA
CATTAGGAAAAAATATTAAA

Downstream 100 bases:

>100_bases
ACTGAGATTGGGTTAAGCCATTCTGATATTTTCACCCATTAGCACATTTTTTTATTGGGTGTTAAATTGATTGATGGCTT
ACCTTTTTGCAGGCAATAAA

Product: DNA ligase (NAD(+))

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 649; Mature: 649

Protein sequence:

>649_residues
MITLSKSQEEVFKKLAVELSMSKLIEACNKSVDFSSLNSNEQLDVLKVTNAFYRSAEQIINDSTYDAFLSEFSTYNPDHP
YLLTVEPEVLADSKTVPLPKKMLSTDKAYSFEEIKKWIDRLLKAAIEVGVSESEIQIKVTPKLDGYAAYDDGISLYTRGD
GARGQDITRAFNKGLQVANNGDRGLGPGEIVIKKSYFDTVLSDKFENSRNIQAAIIAEKKVDESIQKAIDVGACVFFPFL
SLENWIGHYTEILVDFESIVEKMWSAVDYDIDGVVLEVTNETLKEHMGATRHHHRWQIAFKVNAESAEVKVLNVTPQTSR
TGRVTPVAELEPTKLSGATISRATVHHYNMVKTNGVGPGAIVQLVRSGLVIPKIEKVIKAVEPQLAKECPSCGTHLIWES
DHLVCPNKTDCPAQTENTLVHFFKTLGNNDGFGPKVIEKLHEFGIKKIHEIYELKQESFVSFGFGDKTAQNLVEQLQVSR
DVEIEDWRFLAAFGVSRLAGGNCEKLLQHHSLDSLFEATVEDLVQLDGFAQVSADAIVEGLANIKEEFLKLSALNFNLTI
TPKASEKSPGETPVFGKIIVFTGAMTQGSRGDMEKQAKALGAKVAKSVTGKTSLLVTGDKVGANKINAARDKGVQVLSEL
DYLALISTY

Sequences:

>Translated_649_residues
MITLSKSQEEVFKKLAVELSMSKLIEACNKSVDFSSLNSNEQLDVLKVTNAFYRSAEQIINDSTYDAFLSEFSTYNPDHP
YLLTVEPEVLADSKTVPLPKKMLSTDKAYSFEEIKKWIDRLLKAAIEVGVSESEIQIKVTPKLDGYAAYDDGISLYTRGD
GARGQDITRAFNKGLQVANNGDRGLGPGEIVIKKSYFDTVLSDKFENSRNIQAAIIAEKKVDESIQKAIDVGACVFFPFL
SLENWIGHYTEILVDFESIVEKMWSAVDYDIDGVVLEVTNETLKEHMGATRHHHRWQIAFKVNAESAEVKVLNVTPQTSR
TGRVTPVAELEPTKLSGATISRATVHHYNMVKTNGVGPGAIVQLVRSGLVIPKIEKVIKAVEPQLAKECPSCGTHLIWES
DHLVCPNKTDCPAQTENTLVHFFKTLGNNDGFGPKVIEKLHEFGIKKIHEIYELKQESFVSFGFGDKTAQNLVEQLQVSR
DVEIEDWRFLAAFGVSRLAGGNCEKLLQHHSLDSLFEATVEDLVQLDGFAQVSADAIVEGLANIKEEFLKLSALNFNLTI
TPKASEKSPGETPVFGKIIVFTGAMTQGSRGDMEKQAKALGAKVAKSVTGKTSLLVTGDKVGANKINAARDKGVQVLSEL
DYLALISTY
>Mature_649_residues
MITLSKSQEEVFKKLAVELSMSKLIEACNKSVDFSSLNSNEQLDVLKVTNAFYRSAEQIINDSTYDAFLSEFSTYNPDHP
YLLTVEPEVLADSKTVPLPKKMLSTDKAYSFEEIKKWIDRLLKAAIEVGVSESEIQIKVTPKLDGYAAYDDGISLYTRGD
GARGQDITRAFNKGLQVANNGDRGLGPGEIVIKKSYFDTVLSDKFENSRNIQAAIIAEKKVDESIQKAIDVGACVFFPFL
SLENWIGHYTEILVDFESIVEKMWSAVDYDIDGVVLEVTNETLKEHMGATRHHHRWQIAFKVNAESAEVKVLNVTPQTSR
TGRVTPVAELEPTKLSGATISRATVHHYNMVKTNGVGPGAIVQLVRSGLVIPKIEKVIKAVEPQLAKECPSCGTHLIWES
DHLVCPNKTDCPAQTENTLVHFFKTLGNNDGFGPKVIEKLHEFGIKKIHEIYELKQESFVSFGFGDKTAQNLVEQLQVSR
DVEIEDWRFLAAFGVSRLAGGNCEKLLQHHSLDSLFEATVEDLVQLDGFAQVSADAIVEGLANIKEEFLKLSALNFNLTI
TPKASEKSPGETPVFGKIIVFTGAMTQGSRGDMEKQAKALGAKVAKSVTGKTSLLVTGDKVGANKINAARDKGVQVLSEL
DYLALISTY

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=663, Percent_Identity=27.7526395173454, Blast_Score=188, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_PSYIN (A1SX61)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943675.1
- ProteinModelPortal:   A1SX61
- STRING:   A1SX61
- GeneID:   4627122
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_2336
- eggNOG:   COG0272
- HOGENOM:   HBG295055
- OMA:   EDWRFLA
- ProtClustDB:   CLSK831577
- BioCyc:   PING357804:PING_2336-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 71532; Mature: 71532

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 142-142 BINDING 158-158 BINDING 189-189 BINDING 301-301

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLSKSQEEVFKKLAVELSMSKLIEACNKSVDFSSLNSNEQLDVLKVTNAFYRSAEQII
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
NDSTYDAFLSEFSTYNPDHPYLLTVEPEVLADSKTVPLPKKMLSTDKAYSFEEIKKWIDR
CCCHHHHHHHHHHCCCCCCCEEEEECHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHHH
LLKAAIEVGVSESEIQIKVTPKLDGYAAYDDGISLYTRGDGARGQDITRAFNKGLQVANN
HHHHHHHCCCCCCEEEEEEECCCCCEEEECCCEEEEECCCCCCCHHHHHHHHCCCEECCC
GDRGLGPGEIVIKKSYFDTVLSDKFENSRNIQAAIIAEKKVDESIQKAIDVGACVFFPFL
CCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
SLENWIGHYTEILVDFESIVEKMWSAVDYDIDGVVLEVTNETLKEHMGATRHHHRWQIAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHCCCCCCCEEEEEE
KVNAESAEVKVLNVTPQTSRTGRVTPVAELEPTKLSGATISRATVHHYNMVKTNGVGPGA
EECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHEEEEEECCCCHHH
IVQLVRSGLVIPKIEKVIKAVEPQLAKECPSCGTHLIWESDHLVCPNKTDCPAQTENTLV
HHHHHHCCCCCHHHHHHHHHHCHHHHHHCCCCCCEEEECCCCEECCCCCCCCCCHHHHHH
HFFKTLGNNDGFGPKVIEKLHEFGIKKIHEIYELKQESFVSFGFGDKTAQNLVEQLQVSR
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHC
DVEIEDWRFLAAFGVSRLAGGNCEKLLQHHSLDSLFEATVEDLVQLDGFAQVSADAIVEG
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LANIKEEFLKLSALNFNLTITPKASEKSPGETPVFGKIIVFTGAMTQGSRGDMEKQAKAL
HHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHH
GAKVAKSVTGKTSLLVTGDKVGANKINAARDKGVQVLSELDYLALISTY
HHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MITLSKSQEEVFKKLAVELSMSKLIEACNKSVDFSSLNSNEQLDVLKVTNAFYRSAEQII
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
NDSTYDAFLSEFSTYNPDHPYLLTVEPEVLADSKTVPLPKKMLSTDKAYSFEEIKKWIDR
CCCHHHHHHHHHHCCCCCCCEEEEECHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHHH
LLKAAIEVGVSESEIQIKVTPKLDGYAAYDDGISLYTRGDGARGQDITRAFNKGLQVANN
HHHHHHHCCCCCCEEEEEEECCCCCEEEECCCEEEEECCCCCCCHHHHHHHHCCCEECCC
GDRGLGPGEIVIKKSYFDTVLSDKFENSRNIQAAIIAEKKVDESIQKAIDVGACVFFPFL
CCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
SLENWIGHYTEILVDFESIVEKMWSAVDYDIDGVVLEVTNETLKEHMGATRHHHRWQIAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHCCCCCCCEEEEEE
KVNAESAEVKVLNVTPQTSRTGRVTPVAELEPTKLSGATISRATVHHYNMVKTNGVGPGA
EECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHEEEEEECCCCHHH
IVQLVRSGLVIPKIEKVIKAVEPQLAKECPSCGTHLIWESDHLVCPNKTDCPAQTENTLV
HHHHHHCCCCCHHHHHHHHHHCHHHHHHCCCCCCEEEECCCCEECCCCCCCCCCHHHHHH
HFFKTLGNNDGFGPKVIEKLHEFGIKKIHEIYELKQESFVSFGFGDKTAQNLVEQLQVSR
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHC
DVEIEDWRFLAAFGVSRLAGGNCEKLLQHHSLDSLFEATVEDLVQLDGFAQVSADAIVEG
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LANIKEEFLKLSALNFNLTITPKASEKSPGETPVFGKIIVFTGAMTQGSRGDMEKQAKAL
HHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHH
GAKVAKSVTGKTSLLVTGDKVGANKINAARDKGVQVLSELDYLALISTY
HHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA