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The map label for this gene is thiI

Identifier: 119945887

GI number: 119945887

Start: 2709767

End: 2711221

Strand: Reverse

Name: thiI

Synonym: Ping_2227

Alternate gene names: 119945887

Gene position: 2711221-2709767 (Counterclockwise)

Preceding gene: 119945888

Following gene: 119945886

Centisome position: 59.46

GC content: 40.0

Gene sequence:

>1455_bases
ATGAAATTTATCGTAAAACTTTTTCCCGAAATCATGATGAAAAGTCGTCCTGTTCGTAATCGTTTCAGCAAAATATTACA
GGGTAATATCCGTAATGTATTAACGCGTCATGATGAACAGGTTAAAGTTATTTTGGAATGGGATAAGATCATTGTTCGCA
CCGAAAATGAAAGCGCCGAAAATAAAGCCAATTTGATTCTATTATTAAGCAGTACGCCGGGTATTGCACACTTTTTAGAA
GTAACCGAATCTGTATATACAGATTTGCATGACGTTTATGAGCAAACGCTCGCTATGGTGGGTGATTCACTCGATGGAAA
AACCTTTTGTGTGCGTATTAAACGCATCGGTAAACATGATTTCTCTTCAATAGATGCTGAGCGTTATGTGGGCGGCGGCT
TAAATCAGCACACTGATACGCTGGGCGTGAAATTAAAAGGGCCAGAGATTACGATTAATTTAGAAATTAATAACGAAAAA
CTTTTCTTTATTGATAAAAAACATCCTGGTTTAGGCGGTTTCCCATTAGGGACTCAAGAATCTGTTTTATCTTTAATTTC
TGGTGGTTTTGATTCCGGTGTTTCAAGTTATAAGTTAATTAAACGCGGTTCACGTGTGCATTACTGCTTCTTTAATCTTG
GCGGCGGTGCACATGAGATAGGCGTTAAACAGGTTGCTTATCAGTTGTGGAATCGTTTTGGCTCATCACATCGGGTCAAA
TTTATTTCAATTCCCTTTGAACCTGTCGTCGCAGAAATATTAGAAAAAGTAGAAAATGGTCAAATGGGCGTTGTGCTAAA
GCGTATGATGATGCGTGCTGCAACCCTTATGGCGGAACGTTTAGGTGTCGAGGCACTAATTACCGGAGAAGCCTTGGGTC
AAGTTTCCAGTCAAACACTGCGTAATTTATCGGTGATCGACAAAGTCTCTGATATGCTTATTCTGCGGCCGTTGATTGCG
ACCGATAAGCAGGATATTGTCGATTGCGCCCGGGTTATTGGAACAGCCGAAATTTCTGAAACTATTCCCGAGTATTGTGG
GGTTATTTCGCAGCGTCCAACCGTCAAAGCGGTGATGAGAAAAATTGAGAAGCAGGAAGAAAAATTTGATCTGTCACTAA
TCGAACAGGTTGTTAATCAAGCGCCCACAATAGATATTCGTGATATTGCGAAAGCGGTACACAAAGAAATATTAGAAGTT
GAGTCTGTCTCTCAATTTGCAGAAAATGAAATTGTTTTAGATATTCGATCGCTAGATGAAGCAGAAGAATCCCCGCTTGA
TATTGAGGGAGTTACTATTGAACACTTGCCCTTCTTTAAATTATCTAACCAGTTTGAAACACTGCCGCAGGATAAAATCT
ACCTTTTATATTGCGCTCGCGGTGTAATGAGCAAGCTGCAGGCGTTGTATTTAAAAGAATCTGGCTTTGACAATGTTAAG
GTTTACCGGCCTTAA

Upstream 100 bases:

>100_bases
AAATATATTTTCGATCTCATTTTTTCCTGATTAATAGAAGTGAATATTGTCAAACTATTTGCTATAGTCCGCCACCTTAT
TTATCAGCACAGTGGCCACA

Downstream 100 bases:

>100_bases
AAAATAGGCGGTGGCAAACAAGGTGGTAAATATGCCCTGTTTTTTCCTGTCTAGGTACTAACCTGAACGTTTTCTGATAT
TAAAAGGCGCCTATATTGAG

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MKFIVKLFPEIMMKSRPVRNRFSKILQGNIRNVLTRHDEQVKVILEWDKIIVRTENESAENKANLILLLSSTPGIAHFLE
VTESVYTDLHDVYEQTLAMVGDSLDGKTFCVRIKRIGKHDFSSIDAERYVGGGLNQHTDTLGVKLKGPEITINLEINNEK
LFFIDKKHPGLGGFPLGTQESVLSLISGGFDSGVSSYKLIKRGSRVHYCFFNLGGGAHEIGVKQVAYQLWNRFGSSHRVK
FISIPFEPVVAEILEKVENGQMGVVLKRMMMRAATLMAERLGVEALITGEALGQVSSQTLRNLSVIDKVSDMLILRPLIA
TDKQDIVDCARVIGTAEISETIPEYCGVISQRPTVKAVMRKIEKQEEKFDLSLIEQVVNQAPTIDIRDIAKAVHKEILEV
ESVSQFAENEIVLDIRSLDEAEESPLDIEGVTIEHLPFFKLSNQFETLPQDKIYLLYCARGVMSKLQALYLKESGFDNVK
VYRP

Sequences:

>Translated_484_residues
MKFIVKLFPEIMMKSRPVRNRFSKILQGNIRNVLTRHDEQVKVILEWDKIIVRTENESAENKANLILLLSSTPGIAHFLE
VTESVYTDLHDVYEQTLAMVGDSLDGKTFCVRIKRIGKHDFSSIDAERYVGGGLNQHTDTLGVKLKGPEITINLEINNEK
LFFIDKKHPGLGGFPLGTQESVLSLISGGFDSGVSSYKLIKRGSRVHYCFFNLGGGAHEIGVKQVAYQLWNRFGSSHRVK
FISIPFEPVVAEILEKVENGQMGVVLKRMMMRAATLMAERLGVEALITGEALGQVSSQTLRNLSVIDKVSDMLILRPLIA
TDKQDIVDCARVIGTAEISETIPEYCGVISQRPTVKAVMRKIEKQEEKFDLSLIEQVVNQAPTIDIRDIAKAVHKEILEV
ESVSQFAENEIVLDIRSLDEAEESPLDIEGVTIEHLPFFKLSNQFETLPQDKIYLLYCARGVMSKLQALYLKESGFDNVK
VYRP
>Mature_484_residues
MKFIVKLFPEIMMKSRPVRNRFSKILQGNIRNVLTRHDEQVKVILEWDKIIVRTENESAENKANLILLLSSTPGIAHFLE
VTESVYTDLHDVYEQTLAMVGDSLDGKTFCVRIKRIGKHDFSSIDAERYVGGGLNQHTDTLGVKLKGPEITINLEINNEK
LFFIDKKHPGLGGFPLGTQESVLSLISGGFDSGVSSYKLIKRGSRVHYCFFNLGGGAHEIGVKQVAYQLWNRFGSSHRVK
FISIPFEPVVAEILEKVENGQMGVVLKRMMMRAATLMAERLGVEALITGEALGQVSSQTLRNLSVIDKVSDMLILRPLIA
TDKQDIVDCARVIGTAEISETIPEYCGVISQRPTVKAVMRKIEKQEEKFDLSLIEQVVNQAPTIDIRDIAKAVHKEILEV
ESVSQFAENEIVLDIRSLDEAEESPLDIEGVTIEHLPFFKLSNQFETLPQDKIYLLYCARGVMSKLQALYLKESGFDNVK
VYRP

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=484, Percent_Identity=61.9834710743802, Blast_Score=626, Evalue=1e-180,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_PSYIN (A1SWV3)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943567.1
- ProteinModelPortal:   A1SWV3
- STRING:   A1SWV3
- GeneID:   4624405
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_2227
- eggNOG:   COG0301
- HOGENOM:   HBG646072
- OMA:   GYPIGTQ
- ProtClustDB:   PRK01269
- BioCyc:   PING357804:PING_2227-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.250.10
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP; SSF52821 Rhodanese-like

EC number: =2.8.1.4

Molecular weight: Translated: 54382; Mature: 54382

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50206 RHODANESE_3; PS51165 THUMP

Important sites: ACT_SITE 458-458 BINDING 267-267 BINDING 289-289 BINDING 298-298

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIVKLFPEIMMKSRPVRNRFSKILQGNIRNVLTRHDEQVKVILEWDKIIVRTENESAE
CCHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHCCCCCEEEEEEECEEEEEECCCCCC
NKANLILLLSSTPGIAHFLEVTESVYTDLHDVYEQTLAMVGDSLDGKTFCVRIKRIGKHD
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
FSSIDAERYVGGGLNQHTDTLGVKLKGPEITINLEINNEKLFFIDKKHPGLGGFPLGTQE
CCCCCHHHHHCCCCCCCCCCEEEEEECCEEEEEEEECCCEEEEEECCCCCCCCCCCCCHH
SVLSLISGGFDSGVSSYKLIKRGSRVHYCFFNLGGGAHEIGVKQVAYQLWNRFGSSHRVK
HHHHHHHCCCCCCHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEE
FISIPFEPVVAEILEKVENGQMGVVLKRMMMRAATLMAERLGVEALITGEALGQVSSQTL
EEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHH
RNLSVIDKVSDMLILRPLIATDKQDIVDCARVIGTAEISETIPEYCGVISQRPTVKAVMR
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
KIEKQEEKFDLSLIEQVVNQAPTIDIRDIAKAVHKEILEVESVSQFAENEIVLDIRSLDE
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCC
AEESPLDIEGVTIEHLPFFKLSNQFETLPQDKIYLLYCARGVMSKLQALYLKESGFDNVK
CCCCCCCCCCEEECCCCCHHHCCHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEE
VYRP
EECC
>Mature Secondary Structure
MKFIVKLFPEIMMKSRPVRNRFSKILQGNIRNVLTRHDEQVKVILEWDKIIVRTENESAE
CCHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHCCCCCEEEEEEECEEEEEECCCCCC
NKANLILLLSSTPGIAHFLEVTESVYTDLHDVYEQTLAMVGDSLDGKTFCVRIKRIGKHD
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
FSSIDAERYVGGGLNQHTDTLGVKLKGPEITINLEINNEKLFFIDKKHPGLGGFPLGTQE
CCCCCHHHHHCCCCCCCCCCEEEEEECCEEEEEEEECCCEEEEEECCCCCCCCCCCCCHH
SVLSLISGGFDSGVSSYKLIKRGSRVHYCFFNLGGGAHEIGVKQVAYQLWNRFGSSHRVK
HHHHHHHCCCCCCHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEE
FISIPFEPVVAEILEKVENGQMGVVLKRMMMRAATLMAERLGVEALITGEALGQVSSQTL
EEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHH
RNLSVIDKVSDMLILRPLIATDKQDIVDCARVIGTAEISETIPEYCGVISQRPTVKAVMR
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
KIEKQEEKFDLSLIEQVVNQAPTIDIRDIAKAVHKEILEVESVSQFAENEIVLDIRSLDE
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCC
AEESPLDIEGVTIEHLPFFKLSNQFETLPQDKIYLLYCARGVMSKLQALYLKESGFDNVK
CCCCCCCCCCEEECCCCCHHHCCHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEE
VYRP
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA