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The map label for this gene is yebU

Identifier: 119945884

GI number: 119945884

Start: 2706992

End: 2708437

Strand: Direct

Name: yebU

Synonym: Ping_2224

Alternate gene names: 119945884

Gene position: 2706992-2708437 (Clockwise)

Preceding gene: 119945883

Following gene: 119945885

Centisome position: 59.37

GC content: 42.32

Gene sequence:

>1446_bases
ATGCACAGTAAGACCCGACTCCCCCAGGCGTTTATCGATAAGATGACCGGCATTTTACCAAACCAATTGAAGATAGAAGA
TTTTATAGCGGTTTGCCAACGCCCTCTGCGCCGTGCAATCAGAGTCAATACATTAAAAATATCTGTTGAAAAATTTCAAA
TAAGAGCACAGGAACAGGGCTGGGTATTAACAGCAATACCCTGGTGCGCGGAAGGCTTTTGGATTGAACTGCAAGAGGAG
CCAACCCCACTTGGCAACAGTGCAGAACACCTTGGCGGTTTGTGCTATATCCAGGAAGCGAGTTCAATGTTACCCGTCGC
TGCCCTGTTTCATTTTTTCACCCCCACGACACAGTCAATAGTGCTTGATGCGGCGGCGGCCCCAGGGTCTAAAACCACGC
AAATAGCGGCAAAAATGAACAACAGCGGATTGATTATCGGCAATGAATTTTCTTCCAGCAGGATTAAAATGTTACATGCT
AATATTCAACGTTGCGGCATTAAAAACGTGGCCTTAACACATTTTGATGCTCGAGTATTTGGCAAATGGTTACCGGGCAC
CTTTGACGCCATTTTATTAGATGCGCCCTGTTCAGGGGAAGGGACTGTCCGCAAGGATAAATATGCCATGAATAATTGGA
GTCAATCCTCTATTAACGAGATTGCATCCACGCAGCAAGAATTGATCCTCAGTGCTTTTCACGCACTAAAAGAAGACGGA
ATTTTAATCTATTCTACCTGCACATTAAGCCACGAAGAAAATCAGGATATTTGTTTTTTCTTAAAAGATAAATTTCCCGA
GCATATTGAATTTTTAGATTTGGAAACCTTATTTACCGATGCAAAAAAAACCCGCACGGCGGAGGGTTTCTTACATATAT
GGCCACAGGTTTATGACAGTGAAGGCTTTTTTGTAGCGGCCATTAAAAAGACCAAAGCCTGCACTGTTGCACCCGCCGTA
AAACGTTTAGGTAAATTTCCTTTTATCAGGCCAACCAGACAGAAAGAGCAAGCGCTCTATGAGTATTTCGCCAGCCAATT
TGCCATTACAAATATCAAAGGTATGTTATATCAGCGTGATCAGGAATTATGGCTGTTTCCAGATCCTATCAAACCCCTGA
TTAAGGAGTTGCGGTTCTCGCGCTTAGGTATCAAGATTGCCGAAGAATTTGGTAATGGTCGCAAATCAGGCTTTAAAAGC
ACGCATGAATTTGTAACTTGTTTTGGTGATTCGATAGACGCTCACAAATTGGAACTCAGCGCTGAGCAGGCACAAGAATT
CTATCAGGGTCGTGATATTCGCGATATTGACGCTAAGGGGTTTAAAGGTGAAGTCCTATTAAGTTATCGGGGGCATGTAA
TTGGATTAGGAAAATCACTTGATAATCGCATTAAAAACAGTTTACCGCGGGAGTTAATTCGTGATAATAATTTATTTATA
AAATAA

Upstream 100 bases:

>100_bases
GATTAGCTATCCCTAGACCTTAGACTTAGAAATAAAAAATAAAAAATCCGGCAATATCTTATACCGTGCGTATCTCTCGC
GCACATTTAAAGGAATTATA

Downstream 100 bases:

>100_bases
TCTGTTTCTAAAATAATGCGTCTTTAATTTTACAGTTTACATTATTTCACCCCCTATCTTTTAAAAAAGCGAGTTAACAA
AATGTTATTTATTTTAAAAA

Product: rRNA (cytosine-C(5)-)-methyltransferase RsmF

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MHSKTRLPQAFIDKMTGILPNQLKIEDFIAVCQRPLRRAIRVNTLKISVEKFQIRAQEQGWVLTAIPWCAEGFWIELQEE
PTPLGNSAEHLGGLCYIQEASSMLPVAALFHFFTPTTQSIVLDAAAAPGSKTTQIAAKMNNSGLIIGNEFSSSRIKMLHA
NIQRCGIKNVALTHFDARVFGKWLPGTFDAILLDAPCSGEGTVRKDKYAMNNWSQSSINEIASTQQELILSAFHALKEDG
ILIYSTCTLSHEENQDICFFLKDKFPEHIEFLDLETLFTDAKKTRTAEGFLHIWPQVYDSEGFFVAAIKKTKACTVAPAV
KRLGKFPFIRPTRQKEQALYEYFASQFAITNIKGMLYQRDQELWLFPDPIKPLIKELRFSRLGIKIAEEFGNGRKSGFKS
THEFVTCFGDSIDAHKLELSAEQAQEFYQGRDIRDIDAKGFKGEVLLSYRGHVIGLGKSLDNRIKNSLPRELIRDNNLFI
K

Sequences:

>Translated_481_residues
MHSKTRLPQAFIDKMTGILPNQLKIEDFIAVCQRPLRRAIRVNTLKISVEKFQIRAQEQGWVLTAIPWCAEGFWIELQEE
PTPLGNSAEHLGGLCYIQEASSMLPVAALFHFFTPTTQSIVLDAAAAPGSKTTQIAAKMNNSGLIIGNEFSSSRIKMLHA
NIQRCGIKNVALTHFDARVFGKWLPGTFDAILLDAPCSGEGTVRKDKYAMNNWSQSSINEIASTQQELILSAFHALKEDG
ILIYSTCTLSHEENQDICFFLKDKFPEHIEFLDLETLFTDAKKTRTAEGFLHIWPQVYDSEGFFVAAIKKTKACTVAPAV
KRLGKFPFIRPTRQKEQALYEYFASQFAITNIKGMLYQRDQELWLFPDPIKPLIKELRFSRLGIKIAEEFGNGRKSGFKS
THEFVTCFGDSIDAHKLELSAEQAQEFYQGRDIRDIDAKGFKGEVLLSYRGHVIGLGKSLDNRIKNSLPRELIRDNNLFI
K
>Mature_481_residues
MHSKTRLPQAFIDKMTGILPNQLKIEDFIAVCQRPLRRAIRVNTLKISVEKFQIRAQEQGWVLTAIPWCAEGFWIELQEE
PTPLGNSAEHLGGLCYIQEASSMLPVAALFHFFTPTTQSIVLDAAAAPGSKTTQIAAKMNNSGLIIGNEFSSSRIKMLHA
NIQRCGIKNVALTHFDARVFGKWLPGTFDAILLDAPCSGEGTVRKDKYAMNNWSQSSINEIASTQQELILSAFHALKEDG
ILIYSTCTLSHEENQDICFFLKDKFPEHIEFLDLETLFTDAKKTRTAEGFLHIWPQVYDSEGFFVAAIKKTKACTVAPAV
KRLGKFPFIRPTRQKEQALYEYFASQFAITNIKGMLYQRDQELWLFPDPIKPLIKELRFSRLGIKIAEEFGNGRKSGFKS
THEFVTCFGDSIDAHKLELSAEQAQEFYQGRDIRDIDAKGFKGEVLLSYRGHVIGLGKSLDNRIKNSLPRELIRDNNLFI
K

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family

Homologues:

Organism=Homo sapiens, GI76150625, Length=297, Percent_Identity=32.3232323232323, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI76150623, Length=297, Percent_Identity=32.3232323232323, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI32698918, Length=175, Percent_Identity=33.7142857142857, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI270288818, Length=318, Percent_Identity=27.3584905660377, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI8922322, Length=318, Percent_Identity=27.3584905660377, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI11545785, Length=177, Percent_Identity=34.4632768361582, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI23199998, Length=318, Percent_Identity=27.0440251572327, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI270288816, Length=318, Percent_Identity=27.3584905660377, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI39995082, Length=192, Percent_Identity=30.7291666666667, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI301336155, Length=192, Percent_Identity=30.7291666666667, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI40316918, Length=171, Percent_Identity=32.7485380116959, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI87081985, Length=476, Percent_Identity=53.9915966386555, Blast_Score=505, Evalue=1e-144,
Organism=Escherichia coli, GI2367212, Length=177, Percent_Identity=29.9435028248588, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17536757, Length=313, Percent_Identity=31.9488817891374, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI25143471, Length=250, Percent_Identity=28, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324268, Length=290, Percent_Identity=34.8275862068965, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6319447, Length=227, Percent_Identity=29.5154185022026, Blast_Score=93, Evalue=8e-20,
Organism=Drosophila melanogaster, GI22024126, Length=313, Percent_Identity=30.9904153354633, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21355201, Length=183, Percent_Identity=31.1475409836066, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMF_PSYIN (A1SWV0)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943564.1
- ProteinModelPortal:   A1SWV0
- SMR:   A1SWV0
- STRING:   A1SWV0
- GeneID:   4624402
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_2224
- eggNOG:   COG0144
- HOGENOM:   HBG726909
- OMA:   VANEYSA
- PhylomeDB:   A1SWV0
- ProtClustDB:   PRK11933
- BioCyc:   PING357804:PING_2224-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01579
- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023
- TIGRFAMs:   TIGR00446

Pfam domain/function: PF01189 Nol1_Nop2_Fmu

EC number: =2.1.1.178

Molecular weight: Translated: 54378; Mature: 54378

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: ACT_SITE 247-247 BINDING 149-149 BINDING 176-176 BINDING 194-194

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSKTRLPQAFIDKMTGILPNQLKIEDFIAVCQRPLRRAIRVNTLKISVEKFQIRAQEQG
CCCCCCCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHEEEEEEEEEEEEEEEECCCC
WVLTAIPWCAEGFWIELQEEPTPLGNSAEHLGGLCYIQEASSMLPVAALFHFFTPTTQSI
EEEEECCCCCCCEEEEEECCCCCCCCCHHHHCCEEEEECCCCCHHHHHHHHHHCCCCHHE
VLDAAAAPGSKTTQIAAKMNNSGLIIGNEFSSSRIKMLHANIQRCGIKNVALTHFDARVF
EEEECCCCCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHH
GKWLPGTFDAILLDAPCSGEGTVRKDKYAMNNWSQSSINEIASTQQELILSAFHALKEDG
HCCCCCCCCEEEEECCCCCCCCEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
ILIYSTCTLSHEENQDICFFLKDKFPEHIEFLDLETLFTDAKKTRTAEGFLHIWPQVYDS
EEEEEEEEECCCCCCCEEEEEECCCCCHHHHEEHHHHHHHHHHHCCCCCCEEECCEEECC
EGFFVAAIKKTKACTVAPAVKRLGKFPFIRPTRQKEQALYEYFASQFAITNIKGMLYQRD
CCEEEEEECCCCCEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECC
QELWLFPDPIKPLIKELRFSRLGIKIAEEFGNGRKSGFKSTHEFVTCFGDSIDAHKLELS
CCEEECCCCHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEECC
AEQAQEFYQGRDIRDIDAKGFKGEVLLSYRGHVIGLGKSLDNRIKNSLPRELIRDNNLFI
HHHHHHHHCCCCCCCCCCCCCCCEEEEEECCEEEECCCHHHHHHHHHCCHHHHCCCCCEE
K
C
>Mature Secondary Structure
MHSKTRLPQAFIDKMTGILPNQLKIEDFIAVCQRPLRRAIRVNTLKISVEKFQIRAQEQG
CCCCCCCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHEEEEEEEEEEEEEEEECCCC
WVLTAIPWCAEGFWIELQEEPTPLGNSAEHLGGLCYIQEASSMLPVAALFHFFTPTTQSI
EEEEECCCCCCCEEEEEECCCCCCCCCHHHHCCEEEEECCCCCHHHHHHHHHHCCCCHHE
VLDAAAAPGSKTTQIAAKMNNSGLIIGNEFSSSRIKMLHANIQRCGIKNVALTHFDARVF
EEEECCCCCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHH
GKWLPGTFDAILLDAPCSGEGTVRKDKYAMNNWSQSSINEIASTQQELILSAFHALKEDG
HCCCCCCCCEEEEECCCCCCCCEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
ILIYSTCTLSHEENQDICFFLKDKFPEHIEFLDLETLFTDAKKTRTAEGFLHIWPQVYDS
EEEEEEEEECCCCCCCEEEEEECCCCCHHHHEEHHHHHHHHHHHCCCCCCEEECCEEECC
EGFFVAAIKKTKACTVAPAVKRLGKFPFIRPTRQKEQALYEYFASQFAITNIKGMLYQRD
CCEEEEEECCCCCEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECC
QELWLFPDPIKPLIKELRFSRLGIKIAEEFGNGRKSGFKSTHEFVTCFGDSIDAHKLELS
CCEEECCCCHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEECC
AEQAQEFYQGRDIRDIDAKGFKGEVLLSYRGHVIGLGKSLDNRIKNSLPRELIRDNNLFI
HHHHHHHHCCCCCCCCCCCCCCCEEEEEECCEEEECCCHHHHHHHHHCCHHHHCCCCCEE
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA