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The map label for this gene is napF [H]

Identifier: 119945836

GI number: 119945836

Start: 2650887

End: 2651366

Strand: Reverse

Name: napF [H]

Synonym: Ping_2176

Alternate gene names: 119945836

Gene position: 2651366-2650887 (Counterclockwise)

Preceding gene: 119945837

Following gene: 119945835

Centisome position: 58.15

GC content: 38.33

Gene sequence:

>480_bases
GTGAAATTTGATAATAGTAAAAGAAGATTATTTAAAAATAATGACAAAGTGACGGTTAATAATTTATTACCCTGGATTTC
GAATGTAGATTTATTTTTGAAAAATTGTACACAATGTGGCGATTGCATAACCGCTTGCCCGGAAAAAATTATCGTCAAGG
GTGATGGTGGTTACCCCAATATTGATTTTACTGTTGGTGAATGCTCTTTCTGCGGGCAATGTGCTGAAATTTGCGATGAA
CAGATTTTTATCTCTACAAAACAACCACCCTGGTTAAAAAAAGCCCAGATCGACGAATCATGTTTAGCCTATGAAAATGT
CTATTGCCGCAGCTGCAGTGAAAGTTGTGAAGCGCAGGCACTCACTTTCCAAATAGGGTTAAGTGCTGTCCCTCAAATTA
ACCTTGATTTATGTACTGGTTGTGGCGCCTGCATGGCACCTTGTCCAACTGCATCTGTCCTTATAAAGGAATTAGAATGA

Upstream 100 bases:

>100_bases
TCAATAGTGATCAGGTCTGTAAAGATTATTTTTCTCAATCGTTTATTTTATTTAATAATAGATCATTAGGTAATAACCAT
ATAATAAATAAGGTTTTTTA

Downstream 100 bases:

>100_bases
AAACGGAAGAATTGCATATATCGAGTTTAATCGTACATATCAGGCCTGAAAAGCGGCAGGAAATAGAAACAAGCATCAAT
TTATTAGATGGTGCTGAATT

Product: ferredoxin-type protein NapF

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MKFDNSKRRLFKNNDKVTVNNLLPWISNVDLFLKNCTQCGDCITACPEKIIVKGDGGYPNIDFTVGECSFCGQCAEICDE
QIFISTKQPPWLKKAQIDESCLAYENVYCRSCSESCEAQALTFQIGLSAVPQINLDLCTGCGACMAPCPTASVLIKELE

Sequences:

>Translated_159_residues
MKFDNSKRRLFKNNDKVTVNNLLPWISNVDLFLKNCTQCGDCITACPEKIIVKGDGGYPNIDFTVGECSFCGQCAEICDE
QIFISTKQPPWLKKAQIDESCLAYENVYCRSCSESCEAQALTFQIGLSAVPQINLDLCTGCGACMAPCPTASVLIKELE
>Mature_159_residues
MKFDNSKRRLFKNNDKVTVNNLLPWISNVDLFLKNCTQCGDCITACPEKIIVKGDGGYPNIDFTVGECSFCGQCAEICDE
QIFISTKQPPWLKKAQIDESCLAYENVYCRSCSESCEAQALTFQIGLSAVPQINLDLCTGCGACMAPCPTASVLIKELE

Specific function: Involved in electron transfer [H]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788536, Length=162, Percent_Identity=40.1234567901235, Blast_Score=132, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004496 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 17497; Mature: 17497

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
11.3 %Cys+Met (Translated Protein)
10.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
11.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFDNSKRRLFKNNDKVTVNNLLPWISNVDLFLKNCTQCGDCITACPEKIIVKGDGGYPN
CCCCCCCHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
IDFTVGECSFCGQCAEICDEQIFISTKQPPWLKKAQIDESCLAYENVYCRSCSESCEAQA
CEEEECCCHHHHHHHHHCCCEEEEECCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHEE
LTFQIGLSAVPQINLDLCTGCGACMAPCPTASVLIKELE
EEEEECCCCCCCCCHHECCCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MKFDNSKRRLFKNNDKVTVNNLLPWISNVDLFLKNCTQCGDCITACPEKIIVKGDGGYPN
CCCCCCCHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
IDFTVGECSFCGQCAEICDEQIFISTKQPPWLKKAQIDESCLAYENVYCRSCSESCEAQA
CEEEECCCHHHHHHHHHCCCEEEEECCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHEE
LTFQIGLSAVPQINLDLCTGCGACMAPCPTASVLIKELE
EEEEECCCCCCCCCHHECCCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]