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The map label for this gene is ghrA [H]

Identifier: 119945796

GI number: 119945796

Start: 2600494

End: 2601435

Strand: Direct

Name: ghrA [H]

Synonym: Ping_2133

Alternate gene names: 119945796

Gene position: 2600494-2601435 (Clockwise)

Preceding gene: 119945795

Following gene: 119945797

Centisome position: 57.03

GC content: 41.19

Gene sequence:

>942_bases
ATGAAATATATTGTACTGATCACCAATATGGATGCACAAATTGAGCAACAATGGTTAGCGGTATTACAGAAAAGATTACA
CAATGAAAAAATTCTTTTACCCGATCAGATAACAGCTGTTCAGGCACAACAGATTGATATTGCAATAGTGGCCAATCCTG
ATCCGAAAGTTGTTGCACGATTTCCCAATTTAATCTGGATTCAAAGCTTATGGGTTGGTGTTGAAAAGTTGATCACCACA
TTACCTCTGGATAAAATAAAGCTGGTTAAATTAAATGATCCGCACCTTACACAAACAATGGCTGAAGCGGTACTCGCATG
GACACTCTATTTACAGCGCAATATGCCTGAATACACCCTGCAGCAAGCACAAAAAAAATGGCAGCCAATCCCCTATAAAA
GTCCTCAAGAAACACGTGTTTCAATATTGGGTGCGGGTACTTTGAGCATGGCAGCGGTCAATATCTTAAATCGGCATGGT
TACCCTGTTTGTTGTTGGAGCCGCAGCGCAAAAAAACTTACCAGCGGTCACCATTTCACCGGAAAATCCGGCTTGCAGTC
AATGCTACGTAAAACCGATATTTTGATCAATTTACTGCCTTTAACCCCCACTACCCACCACCTTATTGATGATAATTTAT
TAAAGAATTTACCTAAAGGAAGCCGCTTTATAAATTTCTCACGCGCGGCAGTCGTCGATACTAAAGCGTTATTAGCACTA
TTGGATGAGTCACATATTGCACATGCTGTACTGGATGTTTTTGACCAGGAGCCATTGCCCGTTACAAGCAAGATTTGGCA
ACATTCAAACATCACCGTTTTGCCGCATATTTCGGGGCCAACAGATATCAATAGCGCAGCTGGTATTGTGGCTGAAAATA
TAAAAAACTATCGAGCAACAAATATCATTCCCGATGCCGTCGACAGTGAGCAAGGTTATTGA

Upstream 100 bases:

>100_bases
ATTGTAATGATAATCGTTGTACAGCCCATTATGTATAGATAAAGTGGTTGCTTTAGACTTTTGATATTACGCTTTTTTAA
AATAACGAGGTTGTTAATTA

Downstream 100 bases:

>100_bases
TTGTTGATACTGCTTAGCAATAATAGGGCAAGTTTTTAGATAAACTGCTCATAAATAACGCCGATATTTTCAGCCGCTAT
TAACCTCCGGATTAAGACTC

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKYIVLITNMDAQIEQQWLAVLQKRLHNEKILLPDQITAVQAQQIDIAIVANPDPKVVARFPNLIWIQSLWVGVEKLITT
LPLDKIKLVKLNDPHLTQTMAEAVLAWTLYLQRNMPEYTLQQAQKKWQPIPYKSPQETRVSILGAGTLSMAAVNILNRHG
YPVCCWSRSAKKLTSGHHFTGKSGLQSMLRKTDILINLLPLTPTTHHLIDDNLLKNLPKGSRFINFSRAAVVDTKALLAL
LDESHIAHAVLDVFDQEPLPVTSKIWQHSNITVLPHISGPTDINSAAGIVAENIKNYRATNIIPDAVDSEQGY

Sequences:

>Translated_313_residues
MKYIVLITNMDAQIEQQWLAVLQKRLHNEKILLPDQITAVQAQQIDIAIVANPDPKVVARFPNLIWIQSLWVGVEKLITT
LPLDKIKLVKLNDPHLTQTMAEAVLAWTLYLQRNMPEYTLQQAQKKWQPIPYKSPQETRVSILGAGTLSMAAVNILNRHG
YPVCCWSRSAKKLTSGHHFTGKSGLQSMLRKTDILINLLPLTPTTHHLIDDNLLKNLPKGSRFINFSRAAVVDTKALLAL
LDESHIAHAVLDVFDQEPLPVTSKIWQHSNITVLPHISGPTDINSAAGIVAENIKNYRATNIIPDAVDSEQGY
>Mature_313_residues
MKYIVLITNMDAQIEQQWLAVLQKRLHNEKILLPDQITAVQAQQIDIAIVANPDPKVVARFPNLIWIQSLWVGVEKLITT
LPLDKIKLVKLNDPHLTQTMAEAVLAWTLYLQRNMPEYTLQQAQKKWQPIPYKSPQETRVSILGAGTLSMAAVNILNRHG
YPVCCWSRSAKKLTSGHHFTGKSGLQSMLRKTDILINLLPLTPTTHHLIDDNLLKNLPKGSRFINFSRAAVVDTKALLAL
LDESHIAHAVLDVFDQEPLPVTSKIWQHSNITVLPHISGPTDINSAAGIVAENIKNYRATNIIPDAVDSEQGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=194, Percent_Identity=25.7731958762887, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI6912396, Length=231, Percent_Identity=27.7056277056277, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI87081824, Length=274, Percent_Identity=31.7518248175182, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87082289, Length=239, Percent_Identity=28.8702928870293, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789279, Length=191, Percent_Identity=28.7958115183246, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=217, Percent_Identity=24.8847926267281, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324055, Length=171, Percent_Identity=28.0701754385965, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28571528, Length=176, Percent_Identity=28.9772727272727, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 35050; Mature: 35050

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIVLITNMDAQIEQQWLAVLQKRLHNEKILLPDQITAVQAQQIDIAIVANPDPKVVAR
CEEEEEEECCCHHHHHHHHHHHHHHHCCCEEECCCCHHHEEHEEEEEEEEECCCCCCEEC
FPNLIWIQSLWVGVEKLITTLPLDKIKLVKLNDPHLTQTMAEAVLAWTLYLQRNMPEYTL
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHH
QQAQKKWQPIPYKSPQETRVSILGAGTLSMAAVNILNRHGYPVCCWSRSAKKLTSGHHFT
HHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCC
GKSGLQSMLRKTDILINLLPLTPTTHHLIDDNLLKNLPKGSRFINFSRAAVVDTKALLAL
CHHHHHHHHHHHHHEEEEECCCCCHHHHHCCHHHHHCCCCCEEEECCHHHHHHHHHHHHH
LDESHIAHAVLDVFDQEPLPVTSKIWQHSNITVLPHISGPTDINSAAGIVAENIKNYRAT
HHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCC
NIIPDAVDSEQGY
CCCCCCCCCCCCC
>Mature Secondary Structure
MKYIVLITNMDAQIEQQWLAVLQKRLHNEKILLPDQITAVQAQQIDIAIVANPDPKVVAR
CEEEEEEECCCHHHHHHHHHHHHHHHCCCEEECCCCHHHEEHEEEEEEEEECCCCCCEEC
FPNLIWIQSLWVGVEKLITTLPLDKIKLVKLNDPHLTQTMAEAVLAWTLYLQRNMPEYTL
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHH
QQAQKKWQPIPYKSPQETRVSILGAGTLSMAAVNILNRHGYPVCCWSRSAKKLTSGHHFT
HHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCC
GKSGLQSMLRKTDILINLLPLTPTTHHLIDDNLLKNLPKGSRFINFSRAAVVDTKALLAL
CHHHHHHHHHHHHHEEEEECCCCCHHHHHCCHHHHHCCCCCEEEECCHHHHHHHHHHHHH
LDESHIAHAVLDVFDQEPLPVTSKIWQHSNITVLPHISGPTDINSAAGIVAENIKNYRAT
HHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCC
NIIPDAVDSEQGY
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA