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The map label for this gene is opuAB [H]

Identifier: 119945744

GI number: 119945744

Start: 2524009

End: 2524884

Strand: Direct

Name: opuAB [H]

Synonym: Ping_2074

Alternate gene names: 119945744

Gene position: 2524009-2524884 (Clockwise)

Preceding gene: 119945743

Following gene: 119945745

Centisome position: 55.36

GC content: 43.95

Gene sequence:

>876_bases
TTGGCACCCGTCAACCTGAATTTATTTTCATTAAACGGCGTTAAAAATATGAGTTTTATAACAGAGTATAAATTACCTAT
CGGTCAGTGGGCCGCAACTATGATGGATGGGCTAATTACCTACTGCGGTTCTTTTTTTGATGCGATTTCCATGGTACTGG
ATGTCATGATTGAAAGTTTGGTGGATTTTTTATCAATGATGCCACCTTGGCTATTTATTATTATTGTTGCGGGTTTGATT
TTTTGGCAACAGCGCAATAAAGCCGTCACAGCATTTTTTATTTTGTCATTATTACTGATTTGGAATCTCGGCTATTGGGA
AGAAACCATTGAGACCTTTTCTTTAATTATTTACGCCACCGGATTCTGCGTTTTACTGGGTGTCCCCATTGGGATTGCCG
CAGCACATCGTCCTAGGTTGTACCGTTTTATACGGCCCCTGCTGGACTTAATGCAGACGATCCCCCCTTTTGTGTATCTT
ATTCCGGCGTTAACCTTATTTGGCTTGGGCGTGGTGCCGGGTCTTATTTCGACCATAGTGTTCGCCATAGCAGCGCCTAT
CAGGTTGACCCACTTAGGTATTTCCCAAGTACCACTGGAGTTGTTAGAAGCCGGCCGTGCCTTTGGCTGTACTAAGCGCA
GCTTGCTTTTAAAAGTTGAAATTCCAGCGGCTATGTCAAGTATTCGCGCGGGCATTACCCAGTGCATTATGCTGAGTTTA
TCTATGGTCGTGATCTCAGCCTTAGTCGGCGCCGACGGTTTGGGTAAACCCGTGGTACGAGCACTTAATACGGTCAATAT
TGCGACGGGTTTTGAAGCTGGACTCGCCATAGTATTAGTTGCCATCATGCTTGATCGCATATTTAAAAGTAAATAA

Upstream 100 bases:

>100_bases
TTAACGCAGTTAATCGCTCGAAAGGTCATGTTGCCCCTTGCTCTGATCGAAGACCTTGCACCTGACAAAGCATCTTGAAG
TAACATGGGTATATATCAGG

Downstream 100 bases:

>100_bases
ACGACAGGACTTAATAAAATGATTGAATTTAAAAATGTTGATGTTGTTTTTGGCAAGCAACCCGAGCGCGCATTACCGCT
AATGGACAATGGCGAAAGTC

Product: ABC transporter substrate binding inner membrane protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MAPVNLNLFSLNGVKNMSFITEYKLPIGQWAATMMDGLITYCGSFFDAISMVLDVMIESLVDFLSMMPPWLFIIIVAGLI
FWQQRNKAVTAFFILSLLLIWNLGYWEETIETFSLIIYATGFCVLLGVPIGIAAAHRPRLYRFIRPLLDLMQTIPPFVYL
IPALTLFGLGVVPGLISTIVFAIAAPIRLTHLGISQVPLELLEAGRAFGCTKRSLLLKVEIPAAMSSIRAGITQCIMLSL
SMVVISALVGADGLGKPVVRALNTVNIATGFEAGLAIVLVAIMLDRIFKSK

Sequences:

>Translated_291_residues
MAPVNLNLFSLNGVKNMSFITEYKLPIGQWAATMMDGLITYCGSFFDAISMVLDVMIESLVDFLSMMPPWLFIIIVAGLI
FWQQRNKAVTAFFILSLLLIWNLGYWEETIETFSLIIYATGFCVLLGVPIGIAAAHRPRLYRFIRPLLDLMQTIPPFVYL
IPALTLFGLGVVPGLISTIVFAIAAPIRLTHLGISQVPLELLEAGRAFGCTKRSLLLKVEIPAAMSSIRAGITQCIMLSL
SMVVISALVGADGLGKPVVRALNTVNIATGFEAGLAIVLVAIMLDRIFKSK
>Mature_290_residues
APVNLNLFSLNGVKNMSFITEYKLPIGQWAATMMDGLITYCGSFFDAISMVLDVMIESLVDFLSMMPPWLFIIIVAGLIF
WQQRNKAVTAFFILSLLLIWNLGYWEETIETFSLIIYATGFCVLLGVPIGIAAAHRPRLYRFIRPLLDLMQTIPPFVYLI
PALTLFGLGVVPGLISTIVFAIAAPIRLTHLGISQVPLELLEAGRAFGCTKRSLLLKVEIPAAMSSIRAGITQCIMLSLS
MVVISALVGADGLGKPVVRALNTVNIATGFEAGLAIVLVAIMLDRIFKSK

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=266, Percent_Identity=40.2255639097744, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1788449, Length=226, Percent_Identity=28.7610619469027, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788451, Length=195, Percent_Identity=26.1538461538462, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31814; Mature: 31683

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPVNLNLFSLNGVKNMSFITEYKLPIGQWAATMMDGLITYCGSFFDAISMVLDVMIESL
CCCCEEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDFLSMMPPWLFIIIVAGLIFWQQRNKAVTAFFILSLLLIWNLGYWEETIETFSLIIYAT
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GFCVLLGVPIGIAAAHRPRLYRFIRPLLDLMQTIPPFVYLIPALTLFGLGVVPGLISTIV
HHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAIAAPIRLTHLGISQVPLELLEAGRAFGCTKRSLLLKVEIPAAMSSIRAGITQCIMLSL
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHEEEEEECHHHHHHHHHHHHHHHHHHH
SMVVISALVGADGLGKPVVRALNTVNIATGFEAGLAIVLVAIMLDRIFKSK
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
APVNLNLFSLNGVKNMSFITEYKLPIGQWAATMMDGLITYCGSFFDAISMVLDVMIESL
CCCEEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDFLSMMPPWLFIIIVAGLIFWQQRNKAVTAFFILSLLLIWNLGYWEETIETFSLIIYAT
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GFCVLLGVPIGIAAAHRPRLYRFIRPLLDLMQTIPPFVYLIPALTLFGLGVVPGLISTIV
HHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAIAAPIRLTHLGISQVPLELLEAGRAFGCTKRSLLLKVEIPAAMSSIRAGITQCIMLSL
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHEEEEEECHHHHHHHHHHHHHHHHHHH
SMVVISALVGADGLGKPVVRALNTVNIATGFEAGLAIVLVAIMLDRIFKSK
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]