Click here to switch to the map view.

The map label for this gene is rfbX [H]

Identifier: 119945717

GI number: 119945717

Start: 2487157

End: 2488404

Strand: Reverse

Name: rfbX [H]

Synonym: Ping_2041

Alternate gene names: 119945717

Gene position: 2488404-2487157 (Counterclockwise)

Preceding gene: 119945718

Following gene: 119945716

Centisome position: 54.58

GC content: 32.85

Gene sequence:

>1248_bases
ATGTTAGATAGAACAATCATTAAAAATATTTCTTCCCTTTTTAGTATCCGTGTTGCGGCGTATATTGTCCCATTAATCAC
GCTACCTTATCTAGTTCGAGTACTAGAGCCAATAGGATATGGGACACTAGGCTTTAGTTTAGCGATTATTCAGTATTTTG
TTATTGCAGTTAACTTTGGATTTGACTTGTCAGCGACACAAAAAATAGCACAAAGTCCAGATAATAAATTTAAAATAAGC
ACCATTTTTTGGAATGTTATAGCAGCAAGATTTCTTATGTCAGTTACAGGTTTATTTATACTATTTATACTTTCAAATAT
CGCTACTAGTATAGAGATAATATTACCTATACTGTTGTGTGCGTATTTGTCTGTGTTTGGGGCTGCTTTATTTCCACAGT
GGTTGTTTCAAGGAAAAGAACAGTTAGGTACTATTTCAATAATCAGGATTGTACTCCAAGTAATAAGTGTTCCATTTTTA
TTTATCTTTGTAAAGCAAACAGATGATATTTGGGTTGCTGCATTAATCAGCTCAGTTCCATCGTTAATTATCGTAGTTTT
TTCTTCATATTTGATTTTCAAAAGAAAATGGATTGTTTGGCAAACGCCTTCAATTAATCGCATTAGAGGAGAGTTGGCAG
ATGGTTGGCATTTGTTTTTGTCTACGGCAGCGATGAGTCTCTATACAACGAGCACAACAGTAATTTTAGGCATTATTGCA
GGGCCTATAAGTGTAGCAGTTTATGTTTCTGCAAATAAATTGCTACAGGCAGCACAGGGCATATATTCACCAATATCTGT
GTCTTTCTATCCTCGAATTAATAGTTTAATTAGTAAAAGTAAAGATGAAGCGTTAGCTATGATAAGATACTTAATGAAAA
TCCAAATCATATTAACTTGCTGCATTAGTGTTAGCTTATTTTTTTTTGCTCCTTTAGTCGTCGAATTGTTATTTGGTCAG
GAATTCGAAAGAAGTGCTGTAGTACTAAGAATTATGAGTGTGTTACCTATTATAATAGGTCTTAGTAATATTTTTGGTAT
TCAAGTTCTTCTAACGCATGGGTATAAAAAAGAATTCTCAAAAATATTAATCTCTTCCGGTTTTTTTAGTTTGGTTACAT
TGGTACCTTTATGTTATTTATTGGATTCAGAGGGGGCTGCAATATCTGTTATCATCACTGAATCAATAGTCATGTTATTG
ATGTTACGCACTATAAATAAAAAAGAAATCCCCCTCTTTAAACCTTAA

Upstream 100 bases:

>100_bases
ACGACTAAAAAAAAGGCAGGGTAAGGTGCTTCATGATTTCGCCCTGGCCCTTCCCACTGCTACAAGCTGCTCTCATTTCA
TTCAAAAGTTGTAAAATTAG

Downstream 100 bases:

>100_bases
TTTAGAATGTTTTGATAAATGAAATACGATTTTTTAATTGTAGGCTCAGGCCTTTTTGGTTCTGTGTGTGCACATGAATT
AACAAAGCGGGGTAAAAAAG

Product: polysaccharide biosynthesis protein

Products: lipopolysaccharide [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MLDRTIIKNISSLFSIRVAAYIVPLITLPYLVRVLEPIGYGTLGFSLAIIQYFVIAVNFGFDLSATQKIAQSPDNKFKIS
TIFWNVIAARFLMSVTGLFILFILSNIATSIEIILPILLCAYLSVFGAALFPQWLFQGKEQLGTISIIRIVLQVISVPFL
FIFVKQTDDIWVAALISSVPSLIIVVFSSYLIFKRKWIVWQTPSINRIRGELADGWHLFLSTAAMSLYTTSTTVILGIIA
GPISVAVYVSANKLLQAAQGIYSPISVSFYPRINSLISKSKDEALAMIRYLMKIQIILTCCISVSLFFFAPLVVELLFGQ
EFERSAVVLRIMSVLPIIIGLSNIFGIQVLLTHGYKKEFSKILISSGFFSLVTLVPLCYLLDSEGAAISVIITESIVMLL
MLRTINKKEIPLFKP

Sequences:

>Translated_415_residues
MLDRTIIKNISSLFSIRVAAYIVPLITLPYLVRVLEPIGYGTLGFSLAIIQYFVIAVNFGFDLSATQKIAQSPDNKFKIS
TIFWNVIAARFLMSVTGLFILFILSNIATSIEIILPILLCAYLSVFGAALFPQWLFQGKEQLGTISIIRIVLQVISVPFL
FIFVKQTDDIWVAALISSVPSLIIVVFSSYLIFKRKWIVWQTPSINRIRGELADGWHLFLSTAAMSLYTTSTTVILGIIA
GPISVAVYVSANKLLQAAQGIYSPISVSFYPRINSLISKSKDEALAMIRYLMKIQIILTCCISVSLFFFAPLVVELLFGQ
EFERSAVVLRIMSVLPIIIGLSNIFGIQVLLTHGYKKEFSKILISSGFFSLVTLVPLCYLLDSEGAAISVIITESIVMLL
MLRTINKKEIPLFKP
>Mature_415_residues
MLDRTIIKNISSLFSIRVAAYIVPLITLPYLVRVLEPIGYGTLGFSLAIIQYFVIAVNFGFDLSATQKIAQSPDNKFKIS
TIFWNVIAARFLMSVTGLFILFILSNIATSIEIILPILLCAYLSVFGAALFPQWLFQGKEQLGTISIIRIVLQVISVPFL
FIFVKQTDDIWVAALISSVPSLIIVVFSSYLIFKRKWIVWQTPSINRIRGELADGWHLFLSTAAMSLYTTSTTVILGIIA
GPISVAVYVSANKLLQAAQGIYSPISVSFYPRINSLISKSKDEALAMIRYLMKIQIILTCCISVSLFFFAPLVVELLFGQ
EFERSAVVLRIMSVLPIIIGLSNIFGIQVLLTHGYKKEFSKILISSGFFSLVTLVPLCYLLDSEGAAISVIITESIVMLL
MLRTINKKEIPLFKP

Specific function: May be involved in the translocation process of the nascent O-polysaccharide molecules and/or its ligation to lipid A core units [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788349, Length=410, Percent_Identity=36.3414634146341, Blast_Score=265, Evalue=4e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 46166; Mature: 46166

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDRTIIKNISSLFSIRVAAYIVPLITLPYLVRVLEPIGYGTLGFSLAIIQYFVIAVNFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
FDLSATQKIAQSPDNKFKISTIFWNVIAARFLMSVTGLFILFILSNIATSIEIILPILLC
CCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYLSVFGAALFPQWLFQGKEQLGTISIIRIVLQVISVPFLFIFVKQTDDIWVAALISSVP
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHH
SLIIVVFSSYLIFKRKWIVWQTPSINRIRGELADGWHLFLSTAAMSLYTTSTTVILGIIA
HHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GPISVAVYVSANKLLQAAQGIYSPISVSFYPRINSLISKSKDEALAMIRYLMKIQIILTC
HHHHEEEEECHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
CISVSLFFFAPLVVELLFGQEFERSAVVLRIMSVLPIIIGLSNIFGIQVLLTHGYKKEFS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
KILISSGFFSLVTLVPLCYLLDSEGAAISVIITESIVMLLMLRTINKKEIPLFKP
HHHHHCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLDRTIIKNISSLFSIRVAAYIVPLITLPYLVRVLEPIGYGTLGFSLAIIQYFVIAVNFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
FDLSATQKIAQSPDNKFKISTIFWNVIAARFLMSVTGLFILFILSNIATSIEIILPILLC
CCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYLSVFGAALFPQWLFQGKEQLGTISIIRIVLQVISVPFLFIFVKQTDDIWVAALISSVP
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHH
SLIIVVFSSYLIFKRKWIVWQTPSINRIRGELADGWHLFLSTAAMSLYTTSTTVILGIIA
HHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GPISVAVYVSANKLLQAAQGIYSPISVSFYPRINSLISKSKDEALAMIRYLMKIQIILTC
HHHHEEEEECHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
CISVSLFFFAPLVVELLFGQEFERSAVVLRIMSVLPIIIGLSNIFGIQVLLTHGYKKEFS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
KILISSGFFSLVTLVPLCYLLDSEGAAISVIITESIVMLLMLRTINKKEIPLFKP
HHHHHCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipopolysaccharide [Cytoplasm] [C]

Specific reaction: lipopolysaccharide [Cytoplasm] = lipopolysaccharide [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7517390; 7517391; 9097040; 9278503 [H]