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The map label for this gene is yiaO [H]

Identifier: 119945715

GI number: 119945715

Start: 2481159

End: 2482208

Strand: Reverse

Name: yiaO [H]

Synonym: Ping_2035

Alternate gene names: 119945715

Gene position: 2482208-2481159 (Counterclockwise)

Preceding gene: 119945716

Following gene: 119945714

Centisome position: 54.44

GC content: 37.62

Gene sequence:

>1050_bases
ATGAAAAAAACACTTGTTTCATTAATATTAGGACTTACATTATCATCAGCAGCTTATGCTGAAACATGGAAGTTTGCATC
TGAAGAAGCAAAAAATGATGTGCAAGATATATATGCACAAAAATTTGCTGAAGTGATCAAAAAAGAATCTGAGGGAGATA
TTAAGGTTCGTATTTATTATTATGGTCAACTTGGAACTGAAAACGATATAGTTGAATTAGCATCTAACGGAACGATTCAA
TTTGTTTCGGTTGGAGCAGGTCACATAGGCTCTTATATACCAGAAATACAAGCGCTGAGCTTGCCTTATCTCTTAAACGC
TGATGAAGAAGCGGTACATGAAGTATTAACAAACAGCAAGTCAATTTACAATGGTTTATCACCTAAGTTTGAAGCGGTCA
ATCTCAAGCTACTCTCTATGATGTCTGAAGGCGATATGGTGTGGGGAGCAAATAAACCTATTCATACTCCTGCTGATTTT
GAGAATGTTAAAATTAGAACCTTTACATCCGCTATTCCAACGGAAACATATAAAGTTTTTGGTGCAACACCGACTCCTTT
ATCATGGGGTGAAGTATATGGTGCACTACAGCTTGGAACCGTTGATGGCATGGTTAACCCTATCTACTTTATCTATAATG
CTAAGTGGCACGAAGTTCAGGACTACCTGATTTTCCCGGGCCAGCAGCCTTACATCAGTACCGTTTCTGCAAGCAGCAAG
TGGTTTAATAATTTAAGTTCTGAGAAACAAACCATGGTAAACAAGGCGATCACCGCTGCGGATAAAGCTGCGTTTGACTA
TCAAGTGCGTATTAACAGTGAAAACATGGTTAAAATTCGCGAAGAAAGTCCAGACTTAAAAGTAATTGTGCTTAATGAAG
AAGAGCGAGGAGAGTTTATTGAATTGAGTAAATCACTTCATGATACTTATTTTGATCTTGTTGCCAATACCTATCCTGAA
AGTGAACAAACCGCAGCACGTCAAAATGCACAAGATATTGTTAATAACTTAGGAAGAGAAATAAAAGCAGCAGCTAAAAA
CCTCGAGTAA

Upstream 100 bases:

>100_bases
TTTGGGTCAATATTATTGACTTTAGGTGCCGTAACTAGCTAAGTATCTTTTATATCCATACATGGCAAGTCATCTTTTAT
CTTCATACATAGGAATTAAC

Downstream 100 bases:

>100_bases
ATTATTATTGTTGCCAAACTATAGCCCAATCACCGGGCTATTTTTACTTGCATTTTAACAAGGCTGTATTATGAAAAACC
CTCTGTTTATTATAAATCGC

Product: TRAP dicarboxylate transporter- DctP subunit

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKKTLVSLILGLTLSSAAYAETWKFASEEAKNDVQDIYAQKFAEVIKKESEGDIKVRIYYYGQLGTENDIVELASNGTIQ
FVSVGAGHIGSYIPEIQALSLPYLLNADEEAVHEVLTNSKSIYNGLSPKFEAVNLKLLSMMSEGDMVWGANKPIHTPADF
ENVKIRTFTSAIPTETYKVFGATPTPLSWGEVYGALQLGTVDGMVNPIYFIYNAKWHEVQDYLIFPGQQPYISTVSASSK
WFNNLSSEKQTMVNKAITAADKAAFDYQVRINSENMVKIREESPDLKVIVLNEEERGEFIELSKSLHDTYFDLVANTYPE
SEQTAARQNAQDIVNNLGREIKAAAKNLE

Sequences:

>Translated_349_residues
MKKTLVSLILGLTLSSAAYAETWKFASEEAKNDVQDIYAQKFAEVIKKESEGDIKVRIYYYGQLGTENDIVELASNGTIQ
FVSVGAGHIGSYIPEIQALSLPYLLNADEEAVHEVLTNSKSIYNGLSPKFEAVNLKLLSMMSEGDMVWGANKPIHTPADF
ENVKIRTFTSAIPTETYKVFGATPTPLSWGEVYGALQLGTVDGMVNPIYFIYNAKWHEVQDYLIFPGQQPYISTVSASSK
WFNNLSSEKQTMVNKAITAADKAAFDYQVRINSENMVKIREESPDLKVIVLNEEERGEFIELSKSLHDTYFDLVANTYPE
SEQTAARQNAQDIVNNLGREIKAAAKNLE
>Mature_349_residues
MKKTLVSLILGLTLSSAAYAETWKFASEEAKNDVQDIYAQKFAEVIKKESEGDIKVRIYYYGQLGTENDIVELASNGTIQ
FVSVGAGHIGSYIPEIQALSLPYLLNADEEAVHEVLTNSKSIYNGLSPKFEAVNLKLLSMMSEGDMVWGANKPIHTPADF
ENVKIRTFTSAIPTETYKVFGATPTPLSWGEVYGALQLGTVDGMVNPIYFIYNAKWHEVQDYLIFPGQQPYISTVSASSK
WFNNLSSEKQTMVNKAITAADKAAFDYQVRINSENMVKIREESPDLKVIVLNEEERGEFIELSKSLHDTYFDLVANTYPE
SEQTAARQNAQDIVNNLGREIKAAAKNLE

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=309, Percent_Identity=27.1844660194175, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 38913; Mature: 38913

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTLVSLILGLTLSSAAYAETWKFASEEAKNDVQDIYAQKFAEVIKKESEGDIKVRIYY
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
YGQLGTENDIVELASNGTIQFVSVGAGHIGSYIPEIQALSLPYLLNADEEAVHEVLTNSK
EECCCCCCCHHHHCCCCCEEEEEECCCHHHHHCCCHHHCCCCEEECCCHHHHHHHHHCCH
SIYNGLSPKFEAVNLKLLSMMSEGDMVWGANKPIHTPADFENVKIRTFTSAIPTETYKVF
HHHCCCCCCHHHHHHHEEHHHCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCCCEEEE
GATPTPLSWGEVYGALQLGTVDGMVNPIYFIYNAKWHEVQDYLIFPGQQPYISTVSASSK
CCCCCCCCHHHHHCEEEECCCCCCCCCEEEEECCCHHCCCCEEECCCCCCCEEEECCCHH
WFNNLSSEKQTMVNKAITAADKAAFDYQVRINSENMVKIREESPDLKVIVLNEEERGEFI
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCHHH
ELSKSLHDTYFDLVANTYPESEQTAARQNAQDIVNNLGREIKAAAKNLE
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKTLVSLILGLTLSSAAYAETWKFASEEAKNDVQDIYAQKFAEVIKKESEGDIKVRIYY
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
YGQLGTENDIVELASNGTIQFVSVGAGHIGSYIPEIQALSLPYLLNADEEAVHEVLTNSK
EECCCCCCCHHHHCCCCCEEEEEECCCHHHHHCCCHHHCCCCEEECCCHHHHHHHHHCCH
SIYNGLSPKFEAVNLKLLSMMSEGDMVWGANKPIHTPADFENVKIRTFTSAIPTETYKVF
HHHCCCCCCHHHHHHHEEHHHCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCCCEEEE
GATPTPLSWGEVYGALQLGTVDGMVNPIYFIYNAKWHEVQDYLIFPGQQPYISTVSASSK
CCCCCCCCHHHHHCEEEECCCCCCCCCEEEEECCCHHCCCCEEECCCCCCCEEEECCCHH
WFNNLSSEKQTMVNKAITAADKAAFDYQVRINSENMVKIREESPDLKVIVLNEEERGEFI
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCHHH
ELSKSLHDTYFDLVANTYPESEQTAARQNAQDIVNNLGREIKAAAKNLE
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]