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The map label for this gene is hsdS [H]

Identifier: 119945591

GI number: 119945591

Start: 2307232

End: 2309067

Strand: Direct

Name: hsdS [H]

Synonym: Ping_1897

Alternate gene names: 119945591

Gene position: 2307232-2309067 (Clockwise)

Preceding gene: 119945590

Following gene: 119945592

Centisome position: 50.6

GC content: 36.49

Gene sequence:

>1836_bases
ATGCAACAAAAAACCAGTCAGCAAAAGAATTCACTTAAAGACGAGAAGATTAAAAGCAGCAGTATTGAATCTTTAATTAC
AGATAATATAGAGACCTGGACATCAGCCGTTAAGAAAAAATCGGCAACAGGGCGAGGCTCAAATAAAAAAATAGAATTGT
ACGGTATTAAAAAACTGCGTGAACTGATTTTAGAATTGGCGGTGCGCGGTAAGTTAGTAGCACAGGATGCTAATGATGAA
CCAGCTTCGGTTTTACTTGAACGAATTGCAACAGAAAAAGCGCAACTAGTTGCTGATAATAAAATAAAAAATCAAAAAAC
CTTACCAGCTATTACTGATAAAGAAATGCCATTTAATTTACCGGGTGGGTGGGCGTTTGAAAGATTAGGTAATTTAACTT
CTAGATTGGGTTCCGGTAGCACACCTAGAGGTGGAAAAAATGCATATGTTGATAAAGGAGTTATATTTTTACGTTCTCAA
AATGTTTGGAATGATGGTTTAAAACTTGATGATACTGCATATATCTCGGATGAAACGCATCATAAAATGGAAAATACGCG
TGTATTTCCAAATGATGTCTTATTAAATATCACAGGTGCATCACTTGGTCGTTCAACAATTTTTCCCAAAGCTTTAGTAA
CTGCAAATGTTAGTCAGCATGTGACAGTAATAAGGTTAATACATCCAAGTATTTGTCAGTACTTACACTTAGCAATCATG
TCACCTTTAGTTCAAGAATTAGCTTGGGGACGTCAAGTAGGCATGGCAATAGAAGGGCTTAGTAAAAAAGTATTAGAGCA
ATTTGAATTTCCTGTCCCACCATTAGAAGAACAACACCGCATCGTTGCTAAAGTAGATGAACTAATGTTGTTATGCGATC
TGTTTGAACAGAAAACTGAATCCAGTATTGATGCCCATAAAACCTTAGTGGAAGTATTATTAACTACTTTAACCGACAGC
AAAAATTCCGACGAGTTAAATAAAAACTGGGCGCGGGTCAGTGAGTTTTTCGATATCCTTTTTACAACAGAGCACAGTAT
CGATCAGTTAAAGCAAACTATCCTGCAGCTGGCCGTGATGGGTAAACTGGTTGCACAGAATGAAAATGATGAACCCGCTT
CAAAATTACTGGAACGTATCGCCGCCGAAAAAGAAACACTGATTAAAGATAAGAAAATAAAAAAACAAAAAGCATTACCG
CCTATTACGGATGAAGAGAAACCTTTTTCTGTACCAAGTGGGTGGGAGTGGTGTCGGATTTATGATGCTTCATTATTTAC
AGAATATGGAACTTCAGAAAAAGCTTTTGAGGGTAATGATGGAGTTCCTGTTTTAAAAATGGGAGATATACAATCAGGTA
AGGTTTATCACGGAGGCCAAAAAGTTGTCCCGTCAACTATAAAAGACCTACCTAATTTGTATTTGAAATATGGGGATATT
CTTTATAACCGAACTAATAGTGCTGAACTTGTAGGTAAAACGGGTATGTTTGAAGGTGATGATGATATTTTTACATTTGC
ATCATATCTGATTCGTATTAGATGTGACTTTGAAAAAGTAGCTCCTCAATACCTTACATTATCAATGCAAACTCCCTTGT
TCAAAAAAACTCAAATTGATCCGCATGTGAAGCAACAATGTGGGCAAGCTAATGTAAACGGTACCATTATGAAAAGTATG
CTCATTTCCATACCATCGCTATCTGAGCAATATCGCATTGTAAATAAAGTAGAAGAACTAATGACACTTTGTGATCAACT
AAAAACCCGCTTAAACGAATCCCAACAAAGCCAACTTCATCTAGCCGATGCACTTATTGAACAGGCGGTAAATTAA

Upstream 100 bases:

>100_bases
TGCTGGCCAACTACGAAACTCAGCAAAAAGAAATCGGTAACTTGCGCAATCAGCTAAAAAATATTCTCGGGTCGGCACTA
AGCTCGGGAGTGAAATAGCT

Downstream 100 bases:

>100_bases
GATGAGCTTTGATAAACAACATGCCTTAACGGTTTTATCTTTTTGGCAGCAAACCGAGTTTTTTAATACACTTGATTTAA
AAAAATTGCTGCCCGAAGGG

Product: restriction modification system DNA specificity subunit

Products: NA

Alternate protein names: S.EcoEI; Type I restriction enzyme EcoEI specificity protein; S protein [H]

Number of amino acids: Translated: 611; Mature: 611

Protein sequence:

>611_residues
MQQKTSQQKNSLKDEKIKSSSIESLITDNIETWTSAVKKKSATGRGSNKKIELYGIKKLRELILELAVRGKLVAQDANDE
PASVLLERIATEKAQLVADNKIKNQKTLPAITDKEMPFNLPGGWAFERLGNLTSRLGSGSTPRGGKNAYVDKGVIFLRSQ
NVWNDGLKLDDTAYISDETHHKMENTRVFPNDVLLNITGASLGRSTIFPKALVTANVSQHVTVIRLIHPSICQYLHLAIM
SPLVQELAWGRQVGMAIEGLSKKVLEQFEFPVPPLEEQHRIVAKVDELMLLCDLFEQKTESSIDAHKTLVEVLLTTLTDS
KNSDELNKNWARVSEFFDILFTTEHSIDQLKQTILQLAVMGKLVAQNENDEPASKLLERIAAEKETLIKDKKIKKQKALP
PITDEEKPFSVPSGWEWCRIYDASLFTEYGTSEKAFEGNDGVPVLKMGDIQSGKVYHGGQKVVPSTIKDLPNLYLKYGDI
LYNRTNSAELVGKTGMFEGDDDIFTFASYLIRIRCDFEKVAPQYLTLSMQTPLFKKTQIDPHVKQQCGQANVNGTIMKSM
LISIPSLSEQYRIVNKVEELMTLCDQLKTRLNESQQSQLHLADALIEQAVN

Sequences:

>Translated_611_residues
MQQKTSQQKNSLKDEKIKSSSIESLITDNIETWTSAVKKKSATGRGSNKKIELYGIKKLRELILELAVRGKLVAQDANDE
PASVLLERIATEKAQLVADNKIKNQKTLPAITDKEMPFNLPGGWAFERLGNLTSRLGSGSTPRGGKNAYVDKGVIFLRSQ
NVWNDGLKLDDTAYISDETHHKMENTRVFPNDVLLNITGASLGRSTIFPKALVTANVSQHVTVIRLIHPSICQYLHLAIM
SPLVQELAWGRQVGMAIEGLSKKVLEQFEFPVPPLEEQHRIVAKVDELMLLCDLFEQKTESSIDAHKTLVEVLLTTLTDS
KNSDELNKNWARVSEFFDILFTTEHSIDQLKQTILQLAVMGKLVAQNENDEPASKLLERIAAEKETLIKDKKIKKQKALP
PITDEEKPFSVPSGWEWCRIYDASLFTEYGTSEKAFEGNDGVPVLKMGDIQSGKVYHGGQKVVPSTIKDLPNLYLKYGDI
LYNRTNSAELVGKTGMFEGDDDIFTFASYLIRIRCDFEKVAPQYLTLSMQTPLFKKTQIDPHVKQQCGQANVNGTIMKSM
LISIPSLSEQYRIVNKVEELMTLCDQLKTRLNESQQSQLHLADALIEQAVN
>Mature_611_residues
MQQKTSQQKNSLKDEKIKSSSIESLITDNIETWTSAVKKKSATGRGSNKKIELYGIKKLRELILELAVRGKLVAQDANDE
PASVLLERIATEKAQLVADNKIKNQKTLPAITDKEMPFNLPGGWAFERLGNLTSRLGSGSTPRGGKNAYVDKGVIFLRSQ
NVWNDGLKLDDTAYISDETHHKMENTRVFPNDVLLNITGASLGRSTIFPKALVTANVSQHVTVIRLIHPSICQYLHLAIM
SPLVQELAWGRQVGMAIEGLSKKVLEQFEFPVPPLEEQHRIVAKVDELMLLCDLFEQKTESSIDAHKTLVEVLLTTLTDS
KNSDELNKNWARVSEFFDILFTTEHSIDQLKQTILQLAVMGKLVAQNENDEPASKLLERIAAEKETLIKDKKIKKQKALP
PITDEEKPFSVPSGWEWCRIYDASLFTEYGTSEKAFEGNDGVPVLKMGDIQSGKVYHGGQKVVPSTIKDLPNLYLKYGDI
LYNRTNSAELVGKTGMFEGDDDIFTFASYLIRIRCDFEKVAPQYLTLSMQTPLFKKTQIDPHVKQQCGQANVNGTIMKSM
LISIPSLSEQYRIVNKVEELMTLCDQLKTRLNESQQSQLHLADALIEQAVN

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=505, Percent_Identity=20.7920792079208, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 68536; Mature: 68536

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQKTSQQKNSLKDEKIKSSSIESLITDNIETWTSAVKKKSATGRGSNKKIELYGIKKLR
CCCCHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHH
ELILELAVRGKLVAQDANDEPASVLLERIATEKAQLVADNKIKNQKTLPAITDKEMPFNL
HHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PGGWAFERLGNLTSRLGSGSTPRGGKNAYVDKGVIFLRSQNVWNDGLKLDDTAYISDETH
CCCHHHHHHHHHHHHHCCCCCCCCCCCCEECCCEEEEECCCCCCCCCCCCCCEEECCHHH
HKMENTRVFPNDVLLNITGASLGRSTIFPKALVTANVSQHVTVIRLIHPSICQYLHLAIM
HHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
SPLVQELAWGRQVGMAIEGLSKKVLEQFEFPVPPLEEQHRIVAKVDELMLLCDLFEQKTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SSIDAHKTLVEVLLTTLTDSKNSDELNKNWARVSEFFDILFTTEHSIDQLKQTILQLAVM
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GKLVAQNENDEPASKLLERIAAEKETLIKDKKIKKQKALPPITDEEKPFSVPSGWEWCRI
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEE
YDASLFTEYGTSEKAFEGNDGVPVLKMGDIQSGKVYHGGQKVVPSTIKDLPNLYLKYGDI
ECHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHH
LYNRTNSAELVGKTGMFEGDDDIFTFASYLIRIRCDFEKVAPQYLTLSMQTPLFKKTQID
HCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCHHEEEECCCCCCHHCCCC
PHVKQQCGQANVNGTIMKSMLISIPSLSEQYRIVNKVEELMTLCDQLKTRLNESQQSQLH
HHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
LADALIEQAVN
HHHHHHHHHCC
>Mature Secondary Structure
MQQKTSQQKNSLKDEKIKSSSIESLITDNIETWTSAVKKKSATGRGSNKKIELYGIKKLR
CCCCHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHH
ELILELAVRGKLVAQDANDEPASVLLERIATEKAQLVADNKIKNQKTLPAITDKEMPFNL
HHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PGGWAFERLGNLTSRLGSGSTPRGGKNAYVDKGVIFLRSQNVWNDGLKLDDTAYISDETH
CCCHHHHHHHHHHHHHCCCCCCCCCCCCEECCCEEEEECCCCCCCCCCCCCCEEECCHHH
HKMENTRVFPNDVLLNITGASLGRSTIFPKALVTANVSQHVTVIRLIHPSICQYLHLAIM
HHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
SPLVQELAWGRQVGMAIEGLSKKVLEQFEFPVPPLEEQHRIVAKVDELMLLCDLFEQKTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SSIDAHKTLVEVLLTTLTDSKNSDELNKNWARVSEFFDILFTTEHSIDQLKQTILQLAVM
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GKLVAQNENDEPASKLLERIAAEKETLIKDKKIKKQKALPPITDEEKPFSVPSGWEWCRI
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEE
YDASLFTEYGTSEKAFEGNDGVPVLKMGDIQSGKVYHGGQKVVPSTIKDLPNLYLKYGDI
ECHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHH
LYNRTNSAELVGKTGMFEGDDDIFTFASYLIRIRCDFEKVAPQYLTLSMQTPLFKKTQID
HCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCHHEEEECCCCCCHHCCCC
PHVKQQCGQANVNGTIMKSMLISIPSLSEQYRIVNKVEELMTLCDQLKTRLNESQQSQLH
HHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
LADALIEQAVN
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2642743 [H]