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The map label for this gene is tig

Identifier: 119945200

GI number: 119945200

Start: 1808940

End: 1810247

Strand: Direct

Name: tig

Synonym: Ping_1464

Alternate gene names: 119945200

Gene position: 1808940-1810247 (Clockwise)

Preceding gene: 119945199

Following gene: 119945201

Centisome position: 39.67

GC content: 39.22

Gene sequence:

>1308_bases
ATGCAAGTTTCTGTTGAATCAACACAAGGTCTAGAGCGCAAGCTTACTGTCACTATTGCTGCTGAAGCATTTGATAAAGA
GTATAATAGCCGCGTTCATCATTTAGCTAAAACACAACGTGTTGATGGTTTCCGCCCAGGTAAAGTACCCACTTCTGTTG
TCACTAAACGTTTCGGCGCGGGTATTTTCCAAGAAGTGGCTGGTGAATTGATGCAGCGCAACTTTTCTGAAGCGGTTATG
GCTGAAAAACTTAATTTGGCAGCACGCCCTAATTTTGAACCTCAAGCGCGTGAAAAAGGTCAAGACTTTACTTTTACGGC
AACATTTGAAGTTTACCCGGAAGTAACACTAAGCCCATTAGAAGCGTTATCTATTGAGAAAGATTCTGCAGAAGTCACTG
ATGCTGATCTGGATAAAATGATTGAGACGCTGCGTAAGCAACATGCAGAATGGAATGCGGTTGAACGTGAAGCTGCAAAT
GATGACCAGGTAACACTTGATTTTGAAGGCTCTATTGATGGTGAAGTATTCGAAGGCGGAAAAGCCGAAGGATTCGATAT
TGTATTGGGCAGTGGTAAAATGATTCCGGGTTTTGAAGCGGGTATTGTTGGCCATGCGGCTGGCAGTGAGTTTACTATCG
ATGTAAACTTTCCAGAAGATTATCATGCAGAACAACTTAAAGGTAAAGCGGTACAGTTTGCTATTAAACTGACTAAAGTT
GAAGAACAAATTCTGCCAGAAATTACGCCTGAATTTGTACAAAAATTTGGTGTTGAAAACGGCGAGTTAGAAACATTAAA
GCATGATATAAAACAAAATATGGTGCGTGAACTCAGTCAGGCACTTAAAAATAGCGCTAAAGATAAAGTATTAAACGCAC
TTGTTGAAAACAATGAAATTGAAATACCTAAAGCGTTAGTTGAAGACGAAATTGTTGTTTTACGTAAGCAGGCGATGGAA
CGCTACGCTAAAGAAATGGATCCAGAAAACTTACCTGAGTTACCTGCTGAATTGTTCAAAGAACAAGCTGAAAAACGCGT
TAAAGTCGGTTTTCTGTTAGGCGAAGTGATCAACGTTAATGAATTAACCGTTGATCAAGAAAAAGTGGCTGCATTAATTG
AATCTGCCGCATCAGCTTATGATAATCCTGCAGAAGTCATTGCATACTACAAAAATAACAAAGAAATGATGCAGAACATG
GAAAATGTTGCATTAGAAGAGCAAGCAGTTGATTTTATCATCGAAAAAGCTAAAGTAACGGAAGTAAACAAATCGTTCGA
CGAAGTTATGAACAAAGTAGTTGCTTAA

Upstream 100 bases:

>100_bases
TGTTTAAAAACGATAATTCCTTAGAAGTTTAACTCATCACTGAAGGTGGGCTGAACTAGCTAACTTAATTTATTAACGTC
AAAAAGATAAAGGAAATAAA

Downstream 100 bases:

>100_bases
TAGAAACTTATTATTCAATTTTATACCCAGTGTACAGCGCAGAGATGAAACTTTTTTGATTTTTCAGGCGTTTGGGTATT
AGCTTTCAATGGCTCGAGTG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MQVSVESTQGLERKLTVTIAAEAFDKEYNSRVHHLAKTQRVDGFRPGKVPTSVVTKRFGAGIFQEVAGELMQRNFSEAVM
AEKLNLAARPNFEPQAREKGQDFTFTATFEVYPEVTLSPLEALSIEKDSAEVTDADLDKMIETLRKQHAEWNAVEREAAN
DDQVTLDFEGSIDGEVFEGGKAEGFDIVLGSGKMIPGFEAGIVGHAAGSEFTIDVNFPEDYHAEQLKGKAVQFAIKLTKV
EEQILPEITPEFVQKFGVENGELETLKHDIKQNMVRELSQALKNSAKDKVLNALVENNEIEIPKALVEDEIVVLRKQAME
RYAKEMDPENLPELPAELFKEQAEKRVKVGFLLGEVINVNELTVDQEKVAALIESAASAYDNPAEVIAYYKNNKEMMQNM
ENVALEEQAVDFIIEKAKVTEVNKSFDEVMNKVVA

Sequences:

>Translated_435_residues
MQVSVESTQGLERKLTVTIAAEAFDKEYNSRVHHLAKTQRVDGFRPGKVPTSVVTKRFGAGIFQEVAGELMQRNFSEAVM
AEKLNLAARPNFEPQAREKGQDFTFTATFEVYPEVTLSPLEALSIEKDSAEVTDADLDKMIETLRKQHAEWNAVEREAAN
DDQVTLDFEGSIDGEVFEGGKAEGFDIVLGSGKMIPGFEAGIVGHAAGSEFTIDVNFPEDYHAEQLKGKAVQFAIKLTKV
EEQILPEITPEFVQKFGVENGELETLKHDIKQNMVRELSQALKNSAKDKVLNALVENNEIEIPKALVEDEIVVLRKQAME
RYAKEMDPENLPELPAELFKEQAEKRVKVGFLLGEVINVNELTVDQEKVAALIESAASAYDNPAEVIAYYKNNKEMMQNM
ENVALEEQAVDFIIEKAKVTEVNKSFDEVMNKVVA
>Mature_435_residues
MQVSVESTQGLERKLTVTIAAEAFDKEYNSRVHHLAKTQRVDGFRPGKVPTSVVTKRFGAGIFQEVAGELMQRNFSEAVM
AEKLNLAARPNFEPQAREKGQDFTFTATFEVYPEVTLSPLEALSIEKDSAEVTDADLDKMIETLRKQHAEWNAVEREAAN
DDQVTLDFEGSIDGEVFEGGKAEGFDIVLGSGKMIPGFEAGIVGHAAGSEFTIDVNFPEDYHAEQLKGKAVQFAIKLTKV
EEQILPEITPEFVQKFGVENGELETLKHDIKQNMVRELSQALKNSAKDKVLNALVENNEIEIPKALVEDEIVVLRKQAME
RYAKEMDPENLPELPAELFKEQAEKRVKVGFLLGEVINVNELTVDQEKVAALIESAASAYDNPAEVIAYYKNNKEMMQNM
ENVALEEQAVDFIIEKAKVTEVNKSFDEVMNKVVA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=432, Percent_Identity=54.1666666666667, Blast_Score=481, Evalue=1e-137,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_PSYIN (A1SUW6)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942880.1
- ProteinModelPortal:   A1SUW6
- SMR:   A1SUW6
- STRING:   A1SUW6
- GeneID:   4626023
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1464
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   A1SUW6
- BioCyc:   PING357804:PING_1464-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 48533; Mature: 48533

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSVESTQGLERKLTVTIAAEAFDKEYNSRVHHLAKTQRVDGFRPGKVPTSVVTKRFGA
CEECCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC
GIFQEVAGELMQRNFSEAVMAEKLNLAARPNFEPQAREKGQDFTFTATFEVYPEVTLSPL
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEEEEEECCCCCCCHH
EALSIEKDSAEVTDADLDKMIETLRKQHAEWNAVEREAANDDQVTLDFEGSIDGEVFEGG
HHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEECCCCCCCEECCC
KAEGFDIVLGSGKMIPGFEAGIVGHAAGSEFTIDVNFPEDYHAEQLKGKAVQFAIKLTKV
CCCCEEEEEECCCCCCCCCCCEEECCCCCEEEEEECCCCCCCHHHHCCCHHHHHHHHHHH
EEQILPEITPEFVQKFGVENGELETLKHDIKQNMVRELSQALKNSAKDKVLNALVENNEI
HHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EIPKALVEDEIVVLRKQAMERYAKEMDPENLPELPAELFKEQAEKRVKVGFLLGEVINVN
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ELTVDQEKVAALIESAASAYDNPAEVIAYYKNNKEMMQNMENVALEEQAVDFIIEKAKVT
CEECCHHHHHHHHHHHHHCCCCHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVNKSFDEVMNKVVA
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MQVSVESTQGLERKLTVTIAAEAFDKEYNSRVHHLAKTQRVDGFRPGKVPTSVVTKRFGA
CEECCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC
GIFQEVAGELMQRNFSEAVMAEKLNLAARPNFEPQAREKGQDFTFTATFEVYPEVTLSPL
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEEEEEECCCCCCCHH
EALSIEKDSAEVTDADLDKMIETLRKQHAEWNAVEREAANDDQVTLDFEGSIDGEVFEGG
HHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEECCCCCCCEECCC
KAEGFDIVLGSGKMIPGFEAGIVGHAAGSEFTIDVNFPEDYHAEQLKGKAVQFAIKLTKV
CCCCEEEEEECCCCCCCCCCCEEECCCCCEEEEEECCCCCCCHHHHCCCHHHHHHHHHHH
EEQILPEITPEFVQKFGVENGELETLKHDIKQNMVRELSQALKNSAKDKVLNALVENNEI
HHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EIPKALVEDEIVVLRKQAMERYAKEMDPENLPELPAELFKEQAEKRVKVGFLLGEVINVN
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ELTVDQEKVAALIESAASAYDNPAEVIAYYKNNKEMMQNMENVALEEQAVDFIIEKAKVT
CEECCHHHHHHHHHHHHHCCCCHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVNKSFDEVMNKVVA
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA