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The map label for this gene is lutB [H]

Identifier: 119945156

GI number: 119945156

Start: 1743999

End: 1745459

Strand: Reverse

Name: lutB [H]

Synonym: Ping_1420

Alternate gene names: 119945156

Gene position: 1745459-1743999 (Counterclockwise)

Preceding gene: 119945157

Following gene: 119945155

Centisome position: 38.28

GC content: 48.19

Gene sequence:

>1461_bases
ATGAGCCAAGCAAATAACGGCGTACAGATTGAAGATTTAGCACCGGAGCATTTTCATGATCAGGCCAAAGCAGCCCTTGC
CGATCCACAGCTACGCCAAAATTTTCGCGGCGCAATGGACTACTTAACAGACAAACGTAAAATTGCTTTTTCAGATCCGA
CAGAAGAAAAACGCATTCGCGATTTATCCGCGGCGATTCGCCAGCGCAGCCTGAGCAAACTGCCCGATTTACTCGAACAG
CTTGAAACTAACTGTACAAATAATGGTATTCAGGTGCATTGGGCAGAAACGACCGAACAAGCGAACAGTATCATTGCCGG
TATTGCCGGGCAGCATAATGCCAAGAATATTATTAAAGGCAAATCCATGGTGAGTGAAGAAATTGAACTCAACCACGAAA
TGGAAAAGCTCGGTATTGAATGTTTAGAAAGTGATATGGGCGAATATATTGTGCAGCTCGATGGCGATAAACCCTCCCAT
ATCATTATGCCCGCCATTCATAAAAACAAGCAGGAAGTCAGCGATACCTTCGAAAAACACCTCGATGACTTCACCCCGAC
CGTCGATGTGGATGAATTGATTAAAACCGGGCGCACCCGTCTACGGAAAAAATTTCACGATGCAGATATTGGCTTATCGG
GGGTTAACTTTGCCGTCGCGGAAACCGGCACCCTGTGTCTGGTTGAAAACGAAGGCAACGGACGTATGTGTACGACGGTG
CCCAATGTGCATATTGCCATTACCGGTATCGAAAAGGTGGTGGAGTTTTTAAGCGATGTGCCGCCGCTGTACAGCGCCCT
TACCCGTTCAGCTACGGGACAGGCCATTACCACTTATTTTAATATGATAAGCTCACCGCGCCGCGCGGGTGAGTTAGACG
GCCCTGAAGAAGTTCACCTGGTGCTGCTCGATAACGGCCGTACACAAGCCTTTGCCGACGAAGAGATGCGTCAAACACTG
CAATGTATTCGTTGTGGTGCGTGCATGAACCACTGCCCGGTGTACACCCGTATTGGTGGTCATGCTTACGGCACGACTTA
TCCTGGACCCATTGGTCAAATTATTTCACCCCATATGATGGGGCTCGATAACACTAAAGATTTAGTGACAGCATCGAGCC
TGTGCGGTGCCTGTGGCGAAGTCTGCCCGGTAAATATTCCCATTCCAGAAATGCTGCAGCGCTTACGTCAGGAAGCTAAA
AATTCGCCCAAAAATAATAGGCCAGCGATTAAAGGGCAGGCAGCCGCAGCCAGCAGAATAGAGTTATTGGCAATGAAAGG
ATTTGCCTTTGCAGCCTCACACCCTTCTCTTTATCATCTGGGATCATGGTCAGCCACCAAGATGAGATCCTTAATGCCAA
GCAAAATAGGGCCATGGACACAATGCCGAACAGCCCCTAAACCTGCCGGTAAAACCTTACACCAATTAATCAAAGCACGG
AAACAGGGACAAAAATCATGA

Upstream 100 bases:

>100_bases
AATATCGACCATATTGAAAGTCTGTCTCATGTTCAAGCGCACAGGAGAGCGAGCTTCTTATTAGCACACTTCTTATTAGC
ACGGGCGGGAGGAAAATAGA

Downstream 100 bases:

>100_bases
GCAGCGCAAGAAATGCCATTCTATCTCGCCTTCGTGATGCCAAGCCCGAAGCAAAAGCCATGGAAACTATCATATATCAG
CCTTGGGTGAGCGAGGATGA

Product: iron-sulfur cluster binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSQANNGVQIEDLAPEHFHDQAKAALADPQLRQNFRGAMDYLTDKRKIAFSDPTEEKRIRDLSAAIRQRSLSKLPDLLEQ
LETNCTNNGIQVHWAETTEQANSIIAGIAGQHNAKNIIKGKSMVSEEIELNHEMEKLGIECLESDMGEYIVQLDGDKPSH
IIMPAIHKNKQEVSDTFEKHLDDFTPTVDVDELIKTGRTRLRKKFHDADIGLSGVNFAVAETGTLCLVENEGNGRMCTTV
PNVHIAITGIEKVVEFLSDVPPLYSALTRSATGQAITTYFNMISSPRRAGELDGPEEVHLVLLDNGRTQAFADEEMRQTL
QCIRCGACMNHCPVYTRIGGHAYGTTYPGPIGQIISPHMMGLDNTKDLVTASSLCGACGEVCPVNIPIPEMLQRLRQEAK
NSPKNNRPAIKGQAAAASRIELLAMKGFAFAASHPSLYHLGSWSATKMRSLMPSKIGPWTQCRTAPKPAGKTLHQLIKAR
KQGQKS

Sequences:

>Translated_486_residues
MSQANNGVQIEDLAPEHFHDQAKAALADPQLRQNFRGAMDYLTDKRKIAFSDPTEEKRIRDLSAAIRQRSLSKLPDLLEQ
LETNCTNNGIQVHWAETTEQANSIIAGIAGQHNAKNIIKGKSMVSEEIELNHEMEKLGIECLESDMGEYIVQLDGDKPSH
IIMPAIHKNKQEVSDTFEKHLDDFTPTVDVDELIKTGRTRLRKKFHDADIGLSGVNFAVAETGTLCLVENEGNGRMCTTV
PNVHIAITGIEKVVEFLSDVPPLYSALTRSATGQAITTYFNMISSPRRAGELDGPEEVHLVLLDNGRTQAFADEEMRQTL
QCIRCGACMNHCPVYTRIGGHAYGTTYPGPIGQIISPHMMGLDNTKDLVTASSLCGACGEVCPVNIPIPEMLQRLRQEAK
NSPKNNRPAIKGQAAAASRIELLAMKGFAFAASHPSLYHLGSWSATKMRSLMPSKIGPWTQCRTAPKPAGKTLHQLIKAR
KQGQKS
>Mature_485_residues
SQANNGVQIEDLAPEHFHDQAKAALADPQLRQNFRGAMDYLTDKRKIAFSDPTEEKRIRDLSAAIRQRSLSKLPDLLEQL
ETNCTNNGIQVHWAETTEQANSIIAGIAGQHNAKNIIKGKSMVSEEIELNHEMEKLGIECLESDMGEYIVQLDGDKPSHI
IMPAIHKNKQEVSDTFEKHLDDFTPTVDVDELIKTGRTRLRKKFHDADIGLSGVNFAVAETGTLCLVENEGNGRMCTTVP
NVHIAITGIEKVVEFLSDVPPLYSALTRSATGQAITTYFNMISSPRRAGELDGPEEVHLVLLDNGRTQAFADEEMRQTLQ
CIRCGACMNHCPVYTRIGGHAYGTTYPGPIGQIISPHMMGLDNTKDLVTASSLCGACGEVCPVNIPIPEMLQRLRQEAKN
SPKNNRPAIKGQAAAASRIELLAMKGFAFAASHPSLYHLGSWSATKMRSLMPSKIGPWTQCRTAPKPAGKTLHQLIKARK
QGQKS

Specific function: Is essential for L-lactate degradation and allows cells to grow with lactate as the sole carbon source. Has probably a role as an electron transporter during oxidation of L-lactate. May also allow cells to utilize an alternative carbon source during biofi

COG id: COG1139

COG function: function code C; Uncharacterized conserved protein containing a ferredoxin-like domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1786498, Length=477, Percent_Identity=39.622641509434, Blast_Score=369, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017900
- InterPro:   IPR003741
- InterPro:   IPR004452
- InterPro:   IPR002698
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR022825 [H]

Pfam domain/function: PF02589 DUF162 [H]

EC number: NA

Molecular weight: Translated: 53426; Mature: 53294

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQANNGVQIEDLAPEHFHDQAKAALADPQLRQNFRGAMDYLTDKRKIAFSDPTEEKRIR
CCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHH
DLSAAIRQRSLSKLPDLLEQLETNCTNNGIQVHWAETTEQANSIIAGIAGQHNAKNIIKG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCHHHHHH
KSMVSEEIELNHEMEKLGIECLESDMGEYIVQLDGDKPSHIIMPAIHKNKQEVSDTFEKH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEECCHHCCHHHHHHHHHHH
LDDFTPTVDVDELIKTGRTRLRKKFHDADIGLSGVNFAVAETGTLCLVENEGNGRMCTTV
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEEECCCCEEEEEC
PNVHIAITGIEKVVEFLSDVPPLYSALTRSATGQAITTYFNMISSPRRAGELDGPEEVHL
CCEEEEEECHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEE
VLLDNGRTQAFADEEMRQTLQCIRCGACMNHCPVYTRIGGHAYGTTYPGPIGQIISPHMM
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHCCCHHC
GLDNTKDLVTASSLCGACGEVCPVNIPIPEMLQRLRQEAKNSPKNNRPAIKGQAAAASRI
CCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
ELLAMKGFAFAASHPSLYHLGSWSATKMRSLMPSKIGPWTQCRTAPKPAGKTLHQLIKAR
HHHHCCCCEEECCCCCEEECCCCCHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHHH
KQGQKS
HCCCCC
>Mature Secondary Structure 
SQANNGVQIEDLAPEHFHDQAKAALADPQLRQNFRGAMDYLTDKRKIAFSDPTEEKRIR
CCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHH
DLSAAIRQRSLSKLPDLLEQLETNCTNNGIQVHWAETTEQANSIIAGIAGQHNAKNIIKG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCHHHHHH
KSMVSEEIELNHEMEKLGIECLESDMGEYIVQLDGDKPSHIIMPAIHKNKQEVSDTFEKH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEECCHHCCHHHHHHHHHHH
LDDFTPTVDVDELIKTGRTRLRKKFHDADIGLSGVNFAVAETGTLCLVENEGNGRMCTTV
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEEECCCCEEEEEC
PNVHIAITGIEKVVEFLSDVPPLYSALTRSATGQAITTYFNMISSPRRAGELDGPEEVHL
CCEEEEEECHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEE
VLLDNGRTQAFADEEMRQTLQCIRCGACMNHCPVYTRIGGHAYGTTYPGPIGQIISPHMM
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHCCCHHC
GLDNTKDLVTASSLCGACGEVCPVNIPIPEMLQRLRQEAKNSPKNNRPAIKGQAAAASRI
CCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
ELLAMKGFAFAASHPSLYHLGSWSATKMRSLMPSKIGPWTQCRTAPKPAGKTLHQLIKAR
HHHHCCCCEEECCCCCEEECCCCCHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHHH
KQGQKS
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]