Click here to switch to the map view.

The map label for this gene is ogt [H]

Identifier: 119945150

GI number: 119945150

Start: 1734920

End: 1735399

Strand: Direct

Name: ogt [H]

Synonym: Ping_1414

Alternate gene names: 119945150

Gene position: 1734920-1735399 (Clockwise)

Preceding gene: 119945149

Following gene: 119945151

Centisome position: 38.05

GC content: 47.71

Gene sequence:

>480_bases
ATGAACCACTACTGCTATTACAATAGTCCAACCGGTACACTGACCCTGCAGGCAAATGCGCAGGGTTTACTGGGGGTCTG
GTTTGAAACCCAGCATACGCAACCTGAAAACCTCGGGCTGTTGAATAAACAGCAGCCGATATTGATACAGGCAAGAGTGC
AGTTGGAGGAATATTTCAGGGGGGAGCGGGAGTATTTTACGCTGCCGGTAGCTGCTACCGGCACTGTTTTCCAGCAACAA
GTATGGTCTGCTCTTGCGACTATTCCCTTTGGTAAAACATGGTGCTATCAGGAACTTGCCAATGCGATAGGTAACCCTAA
TGCGGTACGCGCAGTCGGCCTTGCAAACAGTAAAAACCCTATTTCGATTATTATTCCATGTCATCGTGTGATTGGCAAAA
ATGGCAAACTGACAGGTTATGCTGGAGGCGTTGAACGTAAACAAGCCTTACTAGAGCATGAAGGGGTCATATTGAGCTGA

Upstream 100 bases:

>100_bases
GTTATTATTTAAATTACTTTTCGTCTTGCTGATGCCTGCATCTTCAAGTAATTTGGGTATAGAGAACTAACACGAGTTTT
TACTGCTGGAATTCAATATT

Downstream 100 bases:

>100_bases
TGACCCTACAGCTGTTTTTTTGGCCACAAGTTGTTTGTTAAGCATATATCAGCAGCTGAATTAGTTTAATTATCAGACTA
CAATATCCCCTATTTTAAAC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MNHYCYYNSPTGTLTLQANAQGLLGVWFETQHTQPENLGLLNKQQPILIQARVQLEEYFRGEREYFTLPVAATGTVFQQQ
VWSALATIPFGKTWCYQELANAIGNPNAVRAVGLANSKNPISIIIPCHRVIGKNGKLTGYAGGVERKQALLEHEGVILS

Sequences:

>Translated_159_residues
MNHYCYYNSPTGTLTLQANAQGLLGVWFETQHTQPENLGLLNKQQPILIQARVQLEEYFRGEREYFTLPVAATGTVFQQQ
VWSALATIPFGKTWCYQELANAIGNPNAVRAVGLANSKNPISIIIPCHRVIGKNGKLTGYAGGVERKQALLEHEGVILS
>Mature_159_residues
MNHYCYYNSPTGTLTLQANAQGLLGVWFETQHTQPENLGLLNKQQPILIQARVQLEEYFRGEREYFTLPVAATGTVFQQQ
VWSALATIPFGKTWCYQELANAIGNPNAVRAVGLANSKNPISIIIPCHRVIGKNGKLTGYAGGVERKQALLEHEGVILS

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=162, Percent_Identity=37.037037037037, Blast_Score=90, Evalue=7e-19,
Organism=Escherichia coli, GI1787596, Length=106, Percent_Identity=57.5471698113208, Blast_Score=125, Evalue=1e-30,
Organism=Escherichia coli, GI1788542, Length=90, Percent_Identity=58.8888888888889, Blast_Score=109, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI115533070, Length=84, Percent_Identity=50, Blast_Score=89, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI115533068, Length=84, Percent_Identity=50, Blast_Score=89, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=41.9753086419753, Blast_Score=78, Evalue=7e-16,
Organism=Drosophila melanogaster, GI17137554, Length=100, Percent_Identity=43, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17584; Mature: 17584

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHYCYYNSPTGTLTLQANAQGLLGVWFETQHTQPENLGLLNKQQPILIQARVQLEEYFR
CCCEEEEECCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEHHHHHHC
GEREYFTLPVAATGTVFQQQVWSALATIPFGKTWCYQELANAIGNPNAVRAVGLANSKNP
CCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCC
ISIIIPCHRVIGKNGKLTGYAGGVERKQALLEHEGVILS
EEEEEECHHHHCCCCCEEEEECCHHHHHHHHHCCCEEEC
>Mature Secondary Structure
MNHYCYYNSPTGTLTLQANAQGLLGVWFETQHTQPENLGLLNKQQPILIQARVQLEEYFR
CCCEEEEECCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEHHHHHHC
GEREYFTLPVAATGTVFQQQVWSALATIPFGKTWCYQELANAIGNPNAVRAVGLANSKNP
CCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCC
ISIIIPCHRVIGKNGKLTGYAGGVERKQALLEHEGVILS
EEEEEECHHHHCCCCCEEEEECCHHHHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]