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The map label for this gene is mrpD [H]

Identifier: 119945099

GI number: 119945099

Start: 1672851

End: 1674362

Strand: Reverse

Name: mrpD [H]

Synonym: Ping_1357

Alternate gene names: 119945099

Gene position: 1674362-1672851 (Counterclockwise)

Preceding gene: 119945100

Following gene: 119945098

Centisome position: 36.72

GC content: 43.65

Gene sequence:

>1512_bases
ATGAGTTTGTTTGACCATTTAGTTATTTTTCCGGTTTTAATACCCTTTTTCGTCGCTGTTGCTCTGCTTTTTCCAAGCTT
AAATAACTCGTTAGCCAAGCAACGGGTATTGACCATTGCTGCCAATATTTTAATGGTCGTTGTTGCGTTTGTGTTGCTAT
TAAAAGTTAAAGACCAGGGGATTCAAGTCTATGCGCTTGGTGATTGGTCCGCGCCTTTTGGCATTGTATTAATGGCCGAT
GCCTTCTCGGTGTTAATGGTCTGCTTAAGTGCCATTTTAGGCTTAGCGGTAACTATGTATGCCAGTGCCGGGGAAGATAA
AAATGGCGCCTTTTTTCACCCACTCATTATGTTCCAATTGATGGGAATTAACGGTGCCTTTTTAACTGGCGATGCATTTA
ACCTATTTGTGTTTTTCGAAGTATTGCTGATTGCCTCATACTCATTAATGATGCACGGAGGTGGCAAACAGCGAATACAG
GCAAACATCCATTATGTATTTCTTAACTTAATTGGCTCGGCGCTGTTTTTGTTTGCGTTAGGTACCTTATATGGCACCTT
AGGTACACTTAACTTTGCAGACATGGCTGATAAAATTCCGCTACTATCGCGCAATGAGTTACTGATCACCAAATCAGGGG
CATTATTGCTATTGGCTGTTTTTGGTTTGAAAGCCGCAATGCTTCCCCTGCATTTCTGGCTACCAAAAGCTTACGCGAGT
ACTAGTGCTCCAGTGGCAGCGCTATTCGCAATTATGACCAAAGTAGGTGTTTATAGTATTTGGCGGGTGCATGGTGTTAT
TTTTGGCGACCAAGCAGGTGAGCTGGCGAACATCGCCTTACCGTGGTTATGGCCTATTGCTTTATTAACCATTGCTATTG
GCGCTATTTCGGTATTGGCTAGCCAAAGTTTGCGGGTGCTTTGTAGTAACTTAGTTATTGTATCAGTAGGCACTCTGCTG
GTGACCATTAGCTTGAATACGGTACAGGCGAGTACAGCTGGTATTTATTACTTACTGCATAGTACCGTAGCTTGTGCCGC
TATGTTCTTGCTAGCGGGATTAATTCAGCAGCAACGCGGTAAAGCGGAAGATCGATTTGTAACCGCCAGGTCAATGCCAC
AGGCAAGCATTTTGGGCTTTATCTTTGCCTTGTTGGCGATAGGTTTAATTGGTATGCCTCCACTGTCAGGCTTTGTAGGT
AAAGCCTTAATTTTACAATCAGTGACCAAAGCAAGCCAGGCAATTTGGATTTGGCCTCTAATATTAGGCGCTGGGCTGAT
TACTTTAATCGCCTTATCACGGGCTGGTACCTCATTGTTTTGGAGAACCACTGGCGAAAACACCAGCGCGCTAAAAGGTC
ATCCTATGCAGTATTTAGCCATTGGCTTATTGGTGGCAATACTCCCTTTGATGGTGATTTTTGGAGGAGCAGTCAGCAAT
TATGCGCAATTGGCAGCAGAGCAACTTAATGCAGGTTTAAGCCTGCAACAACTTAATGAGGTAGCTAAATAA

Upstream 100 bases:

>100_bases
TTGCAGTGATTTTGGCAATGCGTGGACGCTCGGACTTGGGTAATGACCACGTAGATGGTACAGAACCTTTTGACCGCTTA
GCTGTGGAGGAAAAATCCTA

Downstream 100 bases:

>100_bases
TGGATAAAGTGCGTAAGTTTATTTGGTTCCCTACTCCTTACTACAGCATATTGCTATGGTTAGTGTGGCAAATTTTACAT
GATTTTAGTCGTGGTCATAT

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit D; Multiple resistance and pH homeostasis protein D [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MSLFDHLVIFPVLIPFFVAVALLFPSLNNSLAKQRVLTIAANILMVVVAFVLLLKVKDQGIQVYALGDWSAPFGIVLMAD
AFSVLMVCLSAILGLAVTMYASAGEDKNGAFFHPLIMFQLMGINGAFLTGDAFNLFVFFEVLLIASYSLMMHGGGKQRIQ
ANIHYVFLNLIGSALFLFALGTLYGTLGTLNFADMADKIPLLSRNELLITKSGALLLLAVFGLKAAMLPLHFWLPKAYAS
TSAPVAALFAIMTKVGVYSIWRVHGVIFGDQAGELANIALPWLWPIALLTIAIGAISVLASQSLRVLCSNLVIVSVGTLL
VTISLNTVQASTAGIYYLLHSTVACAAMFLLAGLIQQQRGKAEDRFVTARSMPQASILGFIFALLAIGLIGMPPLSGFVG
KALILQSVTKASQAIWIWPLILGAGLITLIALSRAGTSLFWRTTGENTSALKGHPMQYLAIGLLVAILPLMVIFGGAVSN
YAQLAAEQLNAGLSLQQLNEVAK

Sequences:

>Translated_503_residues
MSLFDHLVIFPVLIPFFVAVALLFPSLNNSLAKQRVLTIAANILMVVVAFVLLLKVKDQGIQVYALGDWSAPFGIVLMAD
AFSVLMVCLSAILGLAVTMYASAGEDKNGAFFHPLIMFQLMGINGAFLTGDAFNLFVFFEVLLIASYSLMMHGGGKQRIQ
ANIHYVFLNLIGSALFLFALGTLYGTLGTLNFADMADKIPLLSRNELLITKSGALLLLAVFGLKAAMLPLHFWLPKAYAS
TSAPVAALFAIMTKVGVYSIWRVHGVIFGDQAGELANIALPWLWPIALLTIAIGAISVLASQSLRVLCSNLVIVSVGTLL
VTISLNTVQASTAGIYYLLHSTVACAAMFLLAGLIQQQRGKAEDRFVTARSMPQASILGFIFALLAIGLIGMPPLSGFVG
KALILQSVTKASQAIWIWPLILGAGLITLIALSRAGTSLFWRTTGENTSALKGHPMQYLAIGLLVAILPLMVIFGGAVSN
YAQLAAEQLNAGLSLQQLNEVAK
>Mature_502_residues
SLFDHLVIFPVLIPFFVAVALLFPSLNNSLAKQRVLTIAANILMVVVAFVLLLKVKDQGIQVYALGDWSAPFGIVLMADA
FSVLMVCLSAILGLAVTMYASAGEDKNGAFFHPLIMFQLMGINGAFLTGDAFNLFVFFEVLLIASYSLMMHGGGKQRIQA
NIHYVFLNLIGSALFLFALGTLYGTLGTLNFADMADKIPLLSRNELLITKSGALLLLAVFGLKAAMLPLHFWLPKAYAST
SAPVAALFAIMTKVGVYSIWRVHGVIFGDQAGELANIALPWLWPIALLTIAIGAISVLASQSLRVLCSNLVIVSVGTLLV
TISLNTVQASTAGIYYLLHSTVACAAMFLLAGLIQQQRGKAEDRFVTARSMPQASILGFIFALLAIGLIGMPPLSGFVGK
ALILQSVTKASQAIWIWPLILGAGLITLIALSRAGTSLFWRTTGENTSALKGHPMQYLAIGLLVAILPLMVIFGGAVSNY
AQLAAEQLNAGLSLQQLNEVAK

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788613, Length=358, Percent_Identity=27.3743016759777, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788827, Length=306, Percent_Identity=30.718954248366, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI145693160, Length=407, Percent_Identity=25.0614250614251, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788829, Length=363, Percent_Identity=26.1707988980716, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 53690; Mature: 53559

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFDHLVIFPVLIPFFVAVALLFPSLNNSLAKQRVLTIAANILMVVVAFVLLLKVKDQG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IQVYALGDWSAPFGIVLMADAFSVLMVCLSAILGLAVTMYASAGEDKNGAFFHPLIMFQL
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
MGINGAFLTGDAFNLFVFFEVLLIASYSLMMHGGGKQRIQANIHYVFLNLIGSALFLFAL
HCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHH
GTLYGTLGTLNFADMADKIPLLSRNELLITKSGALLLLAVFGLKAAMLPLHFWLPKAYAS
HHHHHHHHCCCHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHCC
TSAPVAALFAIMTKVGVYSIWRVHGVIFGDQAGELANIALPWLWPIALLTIAIGAISVLA
CCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SQSLRVLCSNLVIVSVGTLLVTISLNTVQASTAGIYYLLHSTVACAAMFLLAGLIQQQRG
HHHHHHHHHHHHHHHHHHEEEEEEECCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KAEDRFVTARSMPQASILGFIFALLAIGLIGMPPLSGFVGKALILQSVTKASQAIWIWPL
CCHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ILGAGLITLIALSRAGTSLFWRTTGENTSALKGHPMQYLAIGLLVAILPLMVIFGGAVSN
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
YAQLAAEQLNAGLSLQQLNEVAK
HHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure 
SLFDHLVIFPVLIPFFVAVALLFPSLNNSLAKQRVLTIAANILMVVVAFVLLLKVKDQG
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IQVYALGDWSAPFGIVLMADAFSVLMVCLSAILGLAVTMYASAGEDKNGAFFHPLIMFQL
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
MGINGAFLTGDAFNLFVFFEVLLIASYSLMMHGGGKQRIQANIHYVFLNLIGSALFLFAL
HCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHH
GTLYGTLGTLNFADMADKIPLLSRNELLITKSGALLLLAVFGLKAAMLPLHFWLPKAYAS
HHHHHHHHCCCHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHCC
TSAPVAALFAIMTKVGVYSIWRVHGVIFGDQAGELANIALPWLWPIALLTIAIGAISVLA
CCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SQSLRVLCSNLVIVSVGTLLVTISLNTVQASTAGIYYLLHSTVACAAMFLLAGLIQQQRG
HHHHHHHHHHHHHHHHHHEEEEEEECCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KAEDRFVTARSMPQASILGFIFALLAIGLIGMPPLSGFVGKALILQSVTKASQAIWIWPL
CCHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ILGAGLITLIALSRAGTSLFWRTTGENTSALKGHPMQYLAIGLLVAILPLMVIFGGAVSN
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
YAQLAAEQLNAGLSLQQLNEVAK
HHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10568751; 10198001; 11356194 [H]