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The map label for this gene is mltB [H]

Identifier: 119944833

GI number: 119944833

Start: 1347730

End: 1348779

Strand: Reverse

Name: mltB [H]

Synonym: Ping_1075

Alternate gene names: 119944833

Gene position: 1348779-1347730 (Counterclockwise)

Preceding gene: 119944834

Following gene: 119944832

Centisome position: 29.58

GC content: 39.24

Gene sequence:

>1050_bases
ATGAAATCTGATAAAAATTTCACTTTATCATCACCAATGAAAATGCTTTTTATGACTTTTTTCAGCAGCTTACTATTTAG
CCAGCTGGCTAATGCAGGTAAAGTTAATGATAATGAAACCCTGCAAAAAATACCATTTGCTGAGTATGTAGAACAGATCA
AAATTGAAGCCAAAGAAAATGGAATTGATCCCGATGTTATCGAAAATGCATTTGCTAACGTTGAGTTTTTACAGCGCAGT
GTTAAATCCGATAAAGAACAACCCGAATTTAAATTAACGCTTGATACTTATATACCAAGAGCCGTGCCCGAGTGGAAAGT
AAAACAAGCACGGAAAGCTTATCAAGAACATTACCAGCTGCTGCATGAAATAGGCAATGCATACGGTGTTCAGCCGCGAT
TTATTGTCGCATTATGGGGTATTGAAACAAATTTTGGCCGTTTTACCGGCAACCATTATATTATCTCCTCTTTAACAACC
ATGGCCTACGAAGGTCGCCGTGAGGCATTATTCAAAAAGCAGTTATTCGCAGCATTAATTATCTTGCAGCAAGGACACGT
AGACCCTGAAGATTTTATCGGTTCATGGGCTGGTGCAATGGGGCAAGTACAATTTATGCCCACTTCTTATCTAAACTATG
CGGTTGATTATGACGGTGATGGTAAAAAAGACATTTGGAATACTAATGCCGATATCTTTGCTTCTGCTGCAAATTACCTG
AAAACAGAGGGTTGGAATAACAATGAGACTTGGGGACGACAAGTCTTATTACCCGATAATTTTGATCTTCAATTGTCCGA
AAGCAAGCAGAAGAAATCACTTCAGGAATGGCAAGATTTAGGTGTCCGACGTTATGACGGAAGTGCTTTACCCAAAGTTG
ATATTAATGCTTCTATCGTTATCCCCGATGACAAAGATGGCCGAATCTATCTTGCATACAATAACTACCATGTTTTAATG
CACTGGAATCGTTCATACTATTTTGCCACGGCAGTGAGTTATCTGTCCGAGCGTATTAATGAGCCGCGTATCGTTGTAAC
AGAGAAGTGA

Upstream 100 bases:

>100_bases
GCAGTGAAAAATAATGGTTATTATCTGCAACTTCCTCCTCCCCCGGTTAATCATCTGCAAGAACACAAAGACTGGAAAAA
AAAACGGCAGGAGAATAAAA

Downstream 100 bases:

>100_bases
TCACTATTTGGTGAACAAAAATGATCCAATAATAGAGTGCAGATCCAATTTAATAAATAAGAGAAATATGAATAAATTCT
GGGAATATAAAACACTATCG

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKSDKNFTLSSPMKMLFMTFFSSLLFSQLANAGKVNDNETLQKIPFAEYVEQIKIEAKENGIDPDVIENAFANVEFLQRS
VKSDKEQPEFKLTLDTYIPRAVPEWKVKQARKAYQEHYQLLHEIGNAYGVQPRFIVALWGIETNFGRFTGNHYIISSLTT
MAYEGRREALFKKQLFAALIILQQGHVDPEDFIGSWAGAMGQVQFMPTSYLNYAVDYDGDGKKDIWNTNADIFASAANYL
KTEGWNNNETWGRQVLLPDNFDLQLSESKQKKSLQEWQDLGVRRYDGSALPKVDINASIVIPDDKDGRIYLAYNNYHVLM
HWNRSYYFATAVSYLSERINEPRIVVTEK

Sequences:

>Translated_349_residues
MKSDKNFTLSSPMKMLFMTFFSSLLFSQLANAGKVNDNETLQKIPFAEYVEQIKIEAKENGIDPDVIENAFANVEFLQRS
VKSDKEQPEFKLTLDTYIPRAVPEWKVKQARKAYQEHYQLLHEIGNAYGVQPRFIVALWGIETNFGRFTGNHYIISSLTT
MAYEGRREALFKKQLFAALIILQQGHVDPEDFIGSWAGAMGQVQFMPTSYLNYAVDYDGDGKKDIWNTNADIFASAANYL
KTEGWNNNETWGRQVLLPDNFDLQLSESKQKKSLQEWQDLGVRRYDGSALPKVDINASIVIPDDKDGRIYLAYNNYHVLM
HWNRSYYFATAVSYLSERINEPRIVVTEK
>Mature_349_residues
MKSDKNFTLSSPMKMLFMTFFSSLLFSQLANAGKVNDNETLQKIPFAEYVEQIKIEAKENGIDPDVIENAFANVEFLQRS
VKSDKEQPEFKLTLDTYIPRAVPEWKVKQARKAYQEHYQLLHEIGNAYGVQPRFIVALWGIETNFGRFTGNHYIISSLTT
MAYEGRREALFKKQLFAALIILQQGHVDPEDFIGSWAGAMGQVQFMPTSYLNYAVDYDGDGKKDIWNTNADIFASAANYL
KTEGWNNNETWGRQVLLPDNFDLQLSESKQKKSLQEWQDLGVRRYDGSALPKVDINASIVIPDDKDGRIYLAYNNYHVLM
HWNRSYYFATAVSYLSERINEPRIVVTEK

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=224, Percent_Identity=31.6964285714286, Blast_Score=108, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 40187; Mature: 40187

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSDKNFTLSSPMKMLFMTFFSSLLFSQLANAGKVNDNETLQKIPFAEYVEQIKIEAKEN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHEEEHHCC
GIDPDVIENAFANVEFLQRSVKSDKEQPEFKLTLDTYIPRAVPEWKVKQARKAYQEHYQL
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHH
LHEIGNAYGVQPRFIVALWGIETNFGRFTGNHYIISSLTTMAYEGRREALFKKQLFAALI
HHHHHHHCCCCCEEEEEEECCCCCCCEECCCEEEEHHHHHHHHCCHHHHHHHHHHHHHHH
ILQQGHVDPEDFIGSWAGAMGQVQFMPTSYLNYAVDYDGDGKKDIWNTNADIFASAANYL
HHHCCCCCHHHHHHHHHCCCCEEEECCHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHH
KTEGWNNNETWGRQVLLPDNFDLQLSESKQKKSLQEWQDLGVRRYDGSALPKVDINASIV
HCCCCCCCCCCCCEEECCCCCCEEECCHHHHHHHHHHHHCCCEEECCCCCCEECCCEEEE
IPDDKDGRIYLAYNNYHVLMHWNRSYYFATAVSYLSERINEPRIVVTEK
EECCCCCEEEEEECCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure
MKSDKNFTLSSPMKMLFMTFFSSLLFSQLANAGKVNDNETLQKIPFAEYVEQIKIEAKEN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHEEEHHCC
GIDPDVIENAFANVEFLQRSVKSDKEQPEFKLTLDTYIPRAVPEWKVKQARKAYQEHYQL
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHH
LHEIGNAYGVQPRFIVALWGIETNFGRFTGNHYIISSLTTMAYEGRREALFKKQLFAALI
HHHHHHHCCCCCEEEEEEECCCCCCCEECCCEEEEHHHHHHHHCCHHHHHHHHHHHHHHH
ILQQGHVDPEDFIGSWAGAMGQVQFMPTSYLNYAVDYDGDGKKDIWNTNADIFASAANYL
HHHCCCCCHHHHHHHHHCCCCEEEECCHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHH
KTEGWNNNETWGRQVLLPDNFDLQLSESKQKKSLQEWQDLGVRRYDGSALPKVDINASIV
HCCCCCCCCCCCCEEECCCCCCEEECCHHHHHHHHHHHHCCCEEECCCCCCEECCCEEEE
IPDDKDGRIYLAYNNYHVLMHWNRSYYFATAVSYLSERINEPRIVVTEK
EECCCCCEEEEEECCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]