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The map label for this gene is trpCF [H]

Identifier: 119944815

GI number: 119944815

Start: 1330985

End: 1332394

Strand: Reverse

Name: trpCF [H]

Synonym: Ping_1057

Alternate gene names: 119944815

Gene position: 1332394-1330985 (Counterclockwise)

Preceding gene: 119944816

Following gene: 119944814

Centisome position: 29.22

GC content: 39.15

Gene sequence:

>1410_bases
ATGTTAAATGATAAAAGCCTAGAGAATACTATTCTGGGAAAAATTATAGCGGATAAGGTCGAGTGGGTAAAAGCGCGTCA
GCAAAAACAACCCCTGATAAGCTTCAAAGATAAAATAACAGAGAGCGATCGCTATTTTTATGAAGCATTAGATAAACCTG
ACACTGTCTTTATTTTAGAGTGTAAAAAAGCCTCCCCGTCGAAAGGGTTAATTCGGGAAAATTTTGATTTGGATCTGATT
GCCGGGACTTATAAAAATTATGCCAATGCAATATCGGTACTGACGGATGAAAAATACTTCCAAGGTGATTTTGAATATAT
CCGTAAAGTTCGGGAGCAAGTATCCCAACCGGTCATTTGTAAAGATTTTATTATTGATCCCTACCAAATTTATCTGGCAC
GCTATTATAAAGCCGATGCGATATTATTAATGCTGTCTGTTTTAGATGATGATGCTTATCAGGCTTATCGCAACACTGCA
CATAGTCTTAATATGGGGGTCTTAACAGAAGTCAGTAATGAGCATGAGCTGGTACGTGCCATTGCATTAAATGCAAAAAT
TATTGGCATAAATAATCGTGACTTACGTGATCTAAGTATTGATTTAAATCGTACCAAAGAGCTGGCAGCTAAAATACCGG
CAGACCGTATTGTTATTTCTGAATCGGGTATTTACAACCATAGTCAGATTAAACAGCTGAGTAAATATGCAAACGGTTTT
TTAGTTGGTAGCGCAATTATGGGCCAACCCAATATTGATAGCGCTTGTAGAAAATTGATTTTAGGCAGTAACAAAGTTTG
CGGTTTAACCCATCCTAGCGCTGCTGCGGATGTTTATAAAGCGGGCGCAGTTTATGGCGGTTTAATCTTTGTTGAAAAGT
CAAAACGTTTTGTGGATATGGAGGAAGCGCGTTTAGTGATGCTCGGTGCACCACTATTATATGTAGGTGTTTTTCAAAAT
GAAGAGCCTGAATTAATCAGCTATACCGTTAAACACTTAGGATTAACGGCTGTACAGTTACACGGTGATGAAAATCCAAA
ATATATTAAAGTGTTACGCGCTTTGTTGCCGGAAAAATGTGAAATATGGAAAGCCCATGGTGTGGTGGATAGTTTGCCTG
ATTTTGAAAAATTTAATGTTCAAAAGCACCTATTAGATACCCGGGTCGGTGAGCAAACAGGGGGAACAGGTAGAGCCTTT
GACTGGACTTTGCTGGACAGTTTAAGCAGTGAGATTAAAAGCAAGATAATACTGGCCGGTGGTTTAACCCCTGAAAATGC
ACAGCAAGCCTGTTTGATAGGCTGCGCAGGGTTAGATTTCAATTCAGGTGTTGAATCTGCACCCGGCAAAAAAGATCCGC
TAAAATTAAACGCCGCATTTTCAGCAATAAAACATTACCTGCAGCGATAA

Upstream 100 bases:

>100_bases
GTAAAGCCGATAACTTTAAAGAGGGCACAAAACAAGCGCTGGCAATGATGTTAACCGGTAAACCATTACAACTATTAAAA
ACACTTGCGGAGCGAAGCCA

Downstream 100 bases:

>100_bases
AATATTATTCATTTATTCTAAGGTGGCATCACCTTGTATAAAATAAAAGGTTATTAAAAATGGAAAAACTAAATCCTTAC
TTTGGTCAATTTGGTGGTAT

Product: bifunctional indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase

Products: NA

Alternate protein names: Indole-3-glycerol phosphate synthase; IGPS; N-(5'-phospho-ribosyl)anthranilate isomerase; PRAI [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MLNDKSLENTILGKIIADKVEWVKARQQKQPLISFKDKITESDRYFYEALDKPDTVFILECKKASPSKGLIRENFDLDLI
AGTYKNYANAISVLTDEKYFQGDFEYIRKVREQVSQPVICKDFIIDPYQIYLARYYKADAILLMLSVLDDDAYQAYRNTA
HSLNMGVLTEVSNEHELVRAIALNAKIIGINNRDLRDLSIDLNRTKELAAKIPADRIVISESGIYNHSQIKQLSKYANGF
LVGSAIMGQPNIDSACRKLILGSNKVCGLTHPSAAADVYKAGAVYGGLIFVEKSKRFVDMEEARLVMLGAPLLYVGVFQN
EEPELISYTVKHLGLTAVQLHGDENPKYIKVLRALLPEKCEIWKAHGVVDSLPDFEKFNVQKHLLDTRVGEQTGGTGRAF
DWTLLDSLSSEIKSKIILAGGLTPENAQQACLIGCAGLDFNSGVESAPGKKDPLKLNAAFSAIKHYLQR

Sequences:

>Translated_469_residues
MLNDKSLENTILGKIIADKVEWVKARQQKQPLISFKDKITESDRYFYEALDKPDTVFILECKKASPSKGLIRENFDLDLI
AGTYKNYANAISVLTDEKYFQGDFEYIRKVREQVSQPVICKDFIIDPYQIYLARYYKADAILLMLSVLDDDAYQAYRNTA
HSLNMGVLTEVSNEHELVRAIALNAKIIGINNRDLRDLSIDLNRTKELAAKIPADRIVISESGIYNHSQIKQLSKYANGF
LVGSAIMGQPNIDSACRKLILGSNKVCGLTHPSAAADVYKAGAVYGGLIFVEKSKRFVDMEEARLVMLGAPLLYVGVFQN
EEPELISYTVKHLGLTAVQLHGDENPKYIKVLRALLPEKCEIWKAHGVVDSLPDFEKFNVQKHLLDTRVGEQTGGTGRAF
DWTLLDSLSSEIKSKIILAGGLTPENAQQACLIGCAGLDFNSGVESAPGKKDPLKLNAAFSAIKHYLQR
>Mature_469_residues
MLNDKSLENTILGKIIADKVEWVKARQQKQPLISFKDKITESDRYFYEALDKPDTVFILECKKASPSKGLIRENFDLDLI
AGTYKNYANAISVLTDEKYFQGDFEYIRKVREQVSQPVICKDFIIDPYQIYLARYYKADAILLMLSVLDDDAYQAYRNTA
HSLNMGVLTEVSNEHELVRAIALNAKIIGINNRDLRDLSIDLNRTKELAAKIPADRIVISESGIYNHSQIKQLSKYANGF
LVGSAIMGQPNIDSACRKLILGSNKVCGLTHPSAAADVYKAGAVYGGLIFVEKSKRFVDMEEARLVMLGAPLLYVGVFQN
EEPELISYTVKHLGLTAVQLHGDENPKYIKVLRALLPEKCEIWKAHGVVDSLPDFEKFNVQKHLLDTRVGEQTGGTGRAF
DWTLLDSLSSEIKSKIILAGGLTPENAQQACLIGCAGLDFNSGVESAPGKKDPLKLNAAFSAIKHYLQR

Specific function: Bifunctional enzyme that catalyzes two sequential steps of tryptophan biosynthetic pathway. The first reaction is catalyzed by the isomerase, coded by the trpF domain; the second reaction is catalyzed by the synthase, coded by the trpC domain [H]

COG id: COG0134

COG function: function code E; Indole-3-glycerol phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the trpF family [H]

Homologues:

Organism=Escherichia coli, GI87081863, Length=458, Percent_Identity=56.5502183406114, Blast_Score=511, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6322638, Length=276, Percent_Identity=34.4202898550725, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320210, Length=213, Percent_Identity=31.924882629108, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013798
- InterPro:   IPR001468
- InterPro:   IPR001240
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00218 IGPS; PF00697 PRAI [H]

EC number: =4.1.1.48; =5.3.1.24 [H]

Molecular weight: Translated: 52287; Mature: 52287

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00614 IGPS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNDKSLENTILGKIIADKVEWVKARQQKQPLISFKDKITESDRYFYEALDKPDTVFILE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHCCCCCEEEEEE
CKKASPSKGLIRENFDLDLIAGTYKNYANAISVLTDEKYFQGDFEYIRKVREQVSQPVIC
ECCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHCCCEEE
KDFIIDPYQIYLARYYKADAILLMLSVLDDDAYQAYRNTAHSLNMGVLTEVSNEHELVRA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEECCCHHHHHHH
IALNAKIIGINNRDLRDLSIDLNRTKELAAKIPADRIVISESGIYNHSQIKQLSKYANGF
HEECEEEEECCCCCCEEEEECCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCE
LVGSAIMGQPNIDSACRKLILGSNKVCGLTHPSAAADVYKAGAVYGGLIFVEKSKRFVDM
EEEEEECCCCCHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHCCEEEEEECCCCEECH
EEARLVMLGAPLLYVGVFQNEEPELISYTVKHLGLTAVQLHGDENPKYIKVLRALLPEKC
HHCCEEEECCCEEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHCCHHH
EIWKAHGVVDSLPDFEKFNVQKHLLDTRVGEQTGGTGRAFDWTLLDSLSSEIKSKIILAG
HHHHHCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCEEEEEC
GLTPENAQQACLIGCAGLDFNSGVESAPGKKDPLKLNAAFSAIKHYLQR
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MLNDKSLENTILGKIIADKVEWVKARQQKQPLISFKDKITESDRYFYEALDKPDTVFILE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHCCCCCEEEEEE
CKKASPSKGLIRENFDLDLIAGTYKNYANAISVLTDEKYFQGDFEYIRKVREQVSQPVIC
ECCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHCCCEEE
KDFIIDPYQIYLARYYKADAILLMLSVLDDDAYQAYRNTAHSLNMGVLTEVSNEHELVRA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEECCCHHHHHHH
IALNAKIIGINNRDLRDLSIDLNRTKELAAKIPADRIVISESGIYNHSQIKQLSKYANGF
HEECEEEEECCCCCCEEEEECCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCE
LVGSAIMGQPNIDSACRKLILGSNKVCGLTHPSAAADVYKAGAVYGGLIFVEKSKRFVDM
EEEEEECCCCCHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHCCEEEEEECCCCEECH
EEARLVMLGAPLLYVGVFQNEEPELISYTVKHLGLTAVQLHGDENPKYIKVLRALLPEKC
HHCCEEEECCCEEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHCCHHH
EIWKAHGVVDSLPDFEKFNVQKHLLDTRVGEQTGGTGRAFDWTLLDSLSSEIKSKIILAG
HHHHHCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCEEEEEC
GLTPENAQQACLIGCAGLDFNSGVESAPGKKDPLKLNAAFSAIKHYLQR
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]