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The map label for this gene is purB [H]

Identifier: 119944721

GI number: 119944721

Start: 1206733

End: 1208106

Strand: Reverse

Name: purB [H]

Synonym: Ping_0961

Alternate gene names: 119944721

Gene position: 1208106-1206733 (Counterclockwise)

Preceding gene: 119944722

Following gene: 119944720

Centisome position: 26.5

GC content: 42.36

Gene sequence:

>1374_bases
ATGGAACTTTCAGGACTAACAGCTGTATCACCAGTTGATGGCCGTTACGGTAGCAAAACCCAACTTTTACGCACCATTTT
CAGCGAATTTGGTTTAATCAAATTCCGCGTGCAAGTTGAGGTTCGCTGGTTACAAAAGCTGGCTGAAACTGCTGATATCG
CAGAAGTTCCTGCTTTCTCAGCGGATGCTAATGCACATCTTGATGCTATTGTATCTGAGTTCAGTGAGGCGGATGCTGCC
CGCATTAAAGCCATTGAAGCGACAACGAACCATGATGTTAAAGCGGTTGAGTACTTCTTAAAAGAGAAAGTTGCAAATGT
TGCTGAATTGAATGCTATTTCGGAATTTATCCACTTTGCCTGTACTTCTGAAGATATTAACAATCTATCTCATGCATTAA
TGCTTACTACCGCTAAAAAAGACGTTATTCTTCCTTACTGCAATAAAATTATTGCGGCGATCGCGGCTAAAGCACATGAA
TATAAAGCAATGCCAATGATGTCCCGTACCCATGGTCAGCCAGCTTCTCCAACAACAATGGGTAAAGAAATGGCTAACGT
TGTTGCACGGTTACGACGTCAAGTAAAACAAATTGAAGCAACAGAAATGTTAGGTAAAACGAATGGCGCGGTGGGTAACT
ACAACGCTCACCTGAGCGCTTACCCAGAAGTGAACTGGGAAGAGTTTGCTGAACAGTTTGTGACCAGCTTAGGGGTGACA
TTCAATCCTTATACGACGCAAATTGAACCACATGACTACATCGCCGAGCTGTTTGATGCGATTGCACGTTTTAATACTAT
TCTTTTAGACTTTGACCGTGACGTATGGGGTTATATCTGCCTTGGCCACTTCAAACAAAAAACCATTGCCGGTGAAATTG
GCTCTTCAACCATGCCGCATAAAGTTAACCCGATTGATTTTGAAAATTCTGAAGGTAACTTAGGCCTAGCAAACGCGCTC
TTTGATCACTTAGCGGCAAAACTGCCCGTTTCTCGCTGGCAGCGTGATTTGACGGATTCAACAGTATTACGTAACTTAGG
TGTTGCAATCGGTTACTCTGTTATTGCCTATGAAGCAAGTTTAAAAGGTATCAGCAAGTTAGAAGCAAACGAAGCAAACA
TGCTAAAAGATCTGGATTCAAACTGGGAAGTATTAGCAGAGCCAATCCAAACAGTAATGCGTCGTTACGGTATTGAAAAA
CCCTATGAAAAATTGAAAGAATTAACCCGTGGCAAACGTGTTGATGGTCCTGGTATGGCGCAGTTTATCGATACACTTGA
TCTCACTGATGCGGTTAAAGCAGAGCTTAAGGCGATGACACCCGCTAATTACATCGGTCAGGCAATAAACTTAGTCGATA
AATTAACTGACTAA

Upstream 100 bases:

>100_bases
TAAGGCGATGATCCACCAAACAAAACAAAATTTACACCGTGTTTGAACATATTTATATAACTATACCTCAACCTGCACTA
CATAATATCAGGAGCAACAA

Downstream 100 bases:

>100_bases
AAGTAATTGAAGAGCAATTTGCTCTTAATATTGTGATTAAATGATAAGGCAGCCTGAGGTTGCCTTTTTTGTTTATTATC
TACTATCCCCAAAGCATCAT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MELSGLTAVSPVDGRYGSKTQLLRTIFSEFGLIKFRVQVEVRWLQKLAETADIAEVPAFSADANAHLDAIVSEFSEADAA
RIKAIEATTNHDVKAVEYFLKEKVANVAELNAISEFIHFACTSEDINNLSHALMLTTAKKDVILPYCNKIIAAIAAKAHE
YKAMPMMSRTHGQPASPTTMGKEMANVVARLRRQVKQIEATEMLGKTNGAVGNYNAHLSAYPEVNWEEFAEQFVTSLGVT
FNPYTTQIEPHDYIAELFDAIARFNTILLDFDRDVWGYICLGHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAL
FDHLAAKLPVSRWQRDLTDSTVLRNLGVAIGYSVIAYEASLKGISKLEANEANMLKDLDSNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDGPGMAQFIDTLDLTDAVKAELKAMTPANYIGQAINLVDKLTD

Sequences:

>Translated_457_residues
MELSGLTAVSPVDGRYGSKTQLLRTIFSEFGLIKFRVQVEVRWLQKLAETADIAEVPAFSADANAHLDAIVSEFSEADAA
RIKAIEATTNHDVKAVEYFLKEKVANVAELNAISEFIHFACTSEDINNLSHALMLTTAKKDVILPYCNKIIAAIAAKAHE
YKAMPMMSRTHGQPASPTTMGKEMANVVARLRRQVKQIEATEMLGKTNGAVGNYNAHLSAYPEVNWEEFAEQFVTSLGVT
FNPYTTQIEPHDYIAELFDAIARFNTILLDFDRDVWGYICLGHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAL
FDHLAAKLPVSRWQRDLTDSTVLRNLGVAIGYSVIAYEASLKGISKLEANEANMLKDLDSNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDGPGMAQFIDTLDLTDAVKAELKAMTPANYIGQAINLVDKLTD
>Mature_457_residues
MELSGLTAVSPVDGRYGSKTQLLRTIFSEFGLIKFRVQVEVRWLQKLAETADIAEVPAFSADANAHLDAIVSEFSEADAA
RIKAIEATTNHDVKAVEYFLKEKVANVAELNAISEFIHFACTSEDINNLSHALMLTTAKKDVILPYCNKIIAAIAAKAHE
YKAMPMMSRTHGQPASPTTMGKEMANVVARLRRQVKQIEATEMLGKTNGAVGNYNAHLSAYPEVNWEEFAEQFVTSLGVT
FNPYTTQIEPHDYIAELFDAIARFNTILLDFDRDVWGYICLGHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAL
FDHLAAKLPVSRWQRDLTDSTVLRNLGVAIGYSVIAYEASLKGISKLEANEANMLKDLDSNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDGPGMAQFIDTLDLTDAVKAELKAMTPANYIGQAINLVDKLTD

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=455, Percent_Identity=75.8241758241758, Blast_Score=737, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50624; Mature: 50624

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSGLTAVSPVDGRYGSKTQLLRTIFSEFGLIKFRVQVEVRWLQKLAETADIAEVPAFS
CCCCCCCEECCCCCCCCCHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHCCHHHCCCCC
ADANAHLDAIVSEFSEADAARIKAIEATTNHDVKAVEYFLKEKVANVAELNAISEFIHFA
CCCCHHHHHHHHHHHHCHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CTSEDINNLSHALMLTTAKKDVILPYCNKIIAAIAAKAHEYKAMPMMSRTHGQPASPTTM
CCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHH
GKEMANVVARLRRQVKQIEATEMLGKTNGAVGNYNAHLSAYPEVNWEEFAEQFVTSLGVT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCC
FNPYTTQIEPHDYIAELFDAIARFNTILLDFDRDVWGYICLGHFKQKTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLANALFDHLAAKLPVSRWQRDLTDSTVLRNLGVAIGYSVIAYEAS
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKGISKLEANEANMLKDLDSNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDGPGMA
HHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHH
QFIDTLDLTDAVKAELKAMTPANYIGQAINLVDKLTD
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELSGLTAVSPVDGRYGSKTQLLRTIFSEFGLIKFRVQVEVRWLQKLAETADIAEVPAFS
CCCCCCCEECCCCCCCCCHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHCCHHHCCCCC
ADANAHLDAIVSEFSEADAARIKAIEATTNHDVKAVEYFLKEKVANVAELNAISEFIHFA
CCCCHHHHHHHHHHHHCHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CTSEDINNLSHALMLTTAKKDVILPYCNKIIAAIAAKAHEYKAMPMMSRTHGQPASPTTM
CCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHH
GKEMANVVARLRRQVKQIEATEMLGKTNGAVGNYNAHLSAYPEVNWEEFAEQFVTSLGVT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCC
FNPYTTQIEPHDYIAELFDAIARFNTILLDFDRDVWGYICLGHFKQKTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLANALFDHLAAKLPVSRWQRDLTDSTVLRNLGVAIGYSVIAYEAS
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKGISKLEANEANMLKDLDSNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDGPGMA
HHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHH
QFIDTLDLTDAVKAELKAMTPANYIGQAINLVDKLTD
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]