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The map label for this gene is prfC

Identifier: 119944568

GI number: 119944568

Start: 1007231

End: 1008817

Strand: Direct

Name: prfC

Synonym: Ping_0806

Alternate gene names: 119944568

Gene position: 1007231-1008817 (Clockwise)

Preceding gene: 119944564

Following gene: 119944569

Centisome position: 22.09

GC content: 41.9

Gene sequence:

>1587_bases
ATGACAAGTAAAGACATTATAGAGAATGTAGAGAAGAGAAGAACATTTGCGATCATCTCTCACCCCGATGCAGGTAAAAC
AACGATCACTGAAAAAGTGTTATTATTTGGCAACGCATTACAAAAAGCAGGGACCGTTAAGGGTAAAAAATCGGGTCAGC
ATGCAAAATCTGATTGGATGGAGATGGAGAAAGATCGTGGTATCTCTATCACTACCTCGGTAATGCAGTTTCCCTATAAA
GACAGATTAGTTAATTTATTAGATACTCCCGGACATGAAGATTTCTCGGAAGATACTTACCGTACTTTAACGGCCGTTGA
TTCCTGTTTAATGGTGATTGACGCTGCAAAAGGTGTTGAGCAACGTACTATTAAATTAATGGAAGTAACCCGTTTACGTG
ATACGCCGATTATCACCTTTATGAATAAGTGCGATCGTGAAACACGGGATCCGGTTGATTTAATGGATGAAGTTGAAGCT
ATCTTAAATATTGCCTGTGCACCTATTACTTGGCCTATTGGAATGGGCAAAGAATTTAAAGGGATTTATGATCTGTTACG
TGATGAAGTGATACTTTATTCATCGGGTCAGGGGCATACCATTCAAGAGGTGCGTATTATTAAAGGGCTGGATAATCCAG
AGCTTGAAAGTGTGCTGCCTAACCATATTCATGATCTGCGTGAAGAGTTAGAATTGGTTGTTGGTGCCTCGCATCAATTT
GATCATGAGCTGTTTTTAAAAGGTCAATTAACACCGGTTTATTTTGGTACTGCACTGGGCAACTTTGGTGTTGATCATGT
TCTGGATGGCCTGGTTGAGTGGGCACCAAAACCCTTATCCCGCGCCGCAAAACAACGTGTTGTTGAGCCCCTTGAAGATG
TTTTTTCCGGATTTATTTTTAAAATCCAAGCAAATATGGATCCTAGACATCGTGATAGGATTGCCTTTATGCGTGTTTGT
GCAGGGAAATATGAAAAAGGCATGAAAATGCATCATGTCCGTACGGGTAAAATGGTCAGTATTTCCGATGCGGTTACCTT
TATGGCGGGTGACAGAACCGCCACCGAAGAGGCGTATCCCGGTGATATTATCGGGTTACACAACCACGGTACTATTCAAA
TTGGTGATACATTTACTAGTGGCGAAACGCTTAAATTCGCCGGTATTCCTAATTTCGCACCGGAAATGTTCCGCCGTATT
CGTTTGCTTGATCCGCTTAAACAAAAACAGCTGTTGAAAGGTTTAATGCAACTCTCTGAAGAGGGGGCAGTACAAGTTTT
CAGACCGCTGCGCAGTAATGATTTAATCGTTGGTGCGGTCGGTGTGCTTCAATTTGAAGTGGTTGTACAGCGTTTAAAAT
CGGAATACAAAGTGGATGCTATTTATGAGGCGATCAGTGTTGCAACGGCTCTCTGGGTTGAAAGTGATAACCCGTTAAAA
ATGGCCGAATTTGAGAAAAAAGCTTACGATAATTTAGCCTTAGACGGCAGTAATAACCTGGCCTACATTGCACCGACTAT
GGTCAATCTAAATCTTGCTATGGAGCGCTATCCGGATATTCGCTTTAGAAAAACCCGCGAGCATTAA

Upstream 100 bases:

>100_bases
ATTGCTTTGCTGAATTTGCTATAATCGCAAAAATATATTCATGAATCCTTTTAAAAATTATTGTGTACCAACTTGTGCAC
AAAATTAGAGATAATAAAGA

Downstream 100 bases:

>100_bases
TTTACTTGTGTTTATTTTTGGGGCTTAGCGCGTTGTCAAGTAGCCTTTGCCTCAGAAAGCAGAATCAAGCTTGTAGGGTG
CGCTTCAGCACCGATTACCT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MTSKDIIENVEKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYK
DRLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEQRTIKLMEVTRLRDTPIITFMNKCDRETRDPVDLMDEVEA
ILNIACAPITWPIGMGKEFKGIYDLLRDEVILYSSGQGHTIQEVRIIKGLDNPELESVLPNHIHDLREELELVVGASHQF
DHELFLKGQLTPVYFGTALGNFGVDHVLDGLVEWAPKPLSRAAKQRVVEPLEDVFSGFIFKIQANMDPRHRDRIAFMRVC
AGKYEKGMKMHHVRTGKMVSISDAVTFMAGDRTATEEAYPGDIIGLHNHGTIQIGDTFTSGETLKFAGIPNFAPEMFRRI
RLLDPLKQKQLLKGLMQLSEEGAVQVFRPLRSNDLIVGAVGVLQFEVVVQRLKSEYKVDAIYEAISVATALWVESDNPLK
MAEFEKKAYDNLALDGSNNLAYIAPTMVNLNLAMERYPDIRFRKTREH

Sequences:

>Translated_528_residues
MTSKDIIENVEKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYK
DRLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEQRTIKLMEVTRLRDTPIITFMNKCDRETRDPVDLMDEVEA
ILNIACAPITWPIGMGKEFKGIYDLLRDEVILYSSGQGHTIQEVRIIKGLDNPELESVLPNHIHDLREELELVVGASHQF
DHELFLKGQLTPVYFGTALGNFGVDHVLDGLVEWAPKPLSRAAKQRVVEPLEDVFSGFIFKIQANMDPRHRDRIAFMRVC
AGKYEKGMKMHHVRTGKMVSISDAVTFMAGDRTATEEAYPGDIIGLHNHGTIQIGDTFTSGETLKFAGIPNFAPEMFRRI
RLLDPLKQKQLLKGLMQLSEEGAVQVFRPLRSNDLIVGAVGVLQFEVVVQRLKSEYKVDAIYEAISVATALWVESDNPLK
MAEFEKKAYDNLALDGSNNLAYIAPTMVNLNLAMERYPDIRFRKTREH
>Mature_527_residues
TSKDIIENVEKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYKD
RLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEQRTIKLMEVTRLRDTPIITFMNKCDRETRDPVDLMDEVEAI
LNIACAPITWPIGMGKEFKGIYDLLRDEVILYSSGQGHTIQEVRIIKGLDNPELESVLPNHIHDLREELELVVGASHQFD
HELFLKGQLTPVYFGTALGNFGVDHVLDGLVEWAPKPLSRAAKQRVVEPLEDVFSGFIFKIQANMDPRHRDRIAFMRVCA
GKYEKGMKMHHVRTGKMVSISDAVTFMAGDRTATEEAYPGDIIGLHNHGTIQIGDTFTSGETLKFAGIPNFAPEMFRRIR
LLDPLKQKQLLKGLMQLSEEGAVQVFRPLRSNDLIVGAVGVLQFEVVVQRLKSEYKVDAIYEAISVATALWVESDNPLKM
AEFEKKAYDNLALDGSNNLAYIAPTMVNLNLAMERYPDIRFRKTREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=508, Percent_Identity=28.3464566929134, Blast_Score=182, Evalue=1e-45,
Organism=Homo sapiens, GI19923640, Length=510, Percent_Identity=26.078431372549, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=27.4111675126904, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI25306287, Length=482, Percent_Identity=25.7261410788382, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI157426893, Length=176, Percent_Identity=30.1136363636364, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI94966754, Length=143, Percent_Identity=30.0699300699301, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI310132016, Length=116, Percent_Identity=32.7586206896552, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI310110807, Length=116, Percent_Identity=32.7586206896552, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI310123363, Length=116, Percent_Identity=32.7586206896552, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=28.8590604026846, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1790835, Length=529, Percent_Identity=74.4801512287335, Blast_Score=827, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=498, Percent_Identity=25.7028112449799, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI48994988, Length=194, Percent_Identity=31.4432989690722, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=31.4102564102564, Blast_Score=89, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=484, Percent_Identity=27.8925619834711, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17556745, Length=486, Percent_Identity=24.2798353909465, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=31.8518518518519, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71988819, Length=137, Percent_Identity=30.6569343065693, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=486, Percent_Identity=28.1893004115226, Blast_Score=175, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6322359, Length=576, Percent_Identity=25, Blast_Score=123, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6323320, Length=155, Percent_Identity=26.4516129032258, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324707, Length=161, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=161, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24582462, Length=476, Percent_Identity=27.7310924369748, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI221458488, Length=496, Percent_Identity=24.1935483870968, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI78706572, Length=502, Percent_Identity=23.5059760956175, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=29.4117647058824, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_PSYIN (A1ST34)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942248.1
- ProteinModelPortal:   A1ST34
- SMR:   A1ST34
- STRING:   A1ST34
- GeneID:   4626379
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0806
- eggNOG:   COG4108
- HOGENOM:   HBG285678
- OMA:   LQFEVVQ
- PhylomeDB:   A1ST34
- ProtClustDB:   PRK00741
- BioCyc:   PING357804:PING_0806-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 59482; Mature: 59351

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSKDIIENVEKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWM
CCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
EMEKDRGISITTSVMQFPYKDRLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVE
HHHHCCCCEEEHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
QRTIKLMEVTRLRDTPIITFMNKCDRETRDPVDLMDEVEAILNIACAPITWPIGMGKEFK
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHH
GIYDLLRDEVILYSSGQGHTIQEVRIIKGLDNPELESVLPNHIHDLREELELVVGASHQF
HHHHHHHCCEEEEECCCCCCHHHHHEECCCCCCHHHHHCHHHHHHHHHHHHHHHCCCCCC
DHELFLKGQLTPVYFGTALGNFGVDHVLDGLVEWAPKPLSRAAKQRVVEPLEDVFSGFIF
CCEEEEECCCCEEEEEHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEE
KIQANMDPRHRDRIAFMRVCAGKYEKGMKMHHVRTGKMVSISDAVTFMAGDRTATEEAYP
EEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECHHEEEEECCCCCCCCCCC
GDIIGLHNHGTIQIGDTFTSGETLKFAGIPNFAPEMFRRIRLLDPLKQKQLLKGLMQLSE
CCEEEECCCCEEEECCEECCCCEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCC
EGAVQVFRPLRSNDLIVGAVGVLQFEVVVQRLKSEYKVDAIYEAISVATALWVESDNPLK
CCHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
MAEFEKKAYDNLALDGSNNLAYIAPTMVNLNLAMERYPDIRFRKTREH
HHHHHHHHHCCEEEECCCCEEEEEEEEEEEEEHHHHCCCCEEECCCCC
>Mature Secondary Structure 
TSKDIIENVEKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWM
CCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
EMEKDRGISITTSVMQFPYKDRLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVE
HHHHCCCCEEEHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
QRTIKLMEVTRLRDTPIITFMNKCDRETRDPVDLMDEVEAILNIACAPITWPIGMGKEFK
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHH
GIYDLLRDEVILYSSGQGHTIQEVRIIKGLDNPELESVLPNHIHDLREELELVVGASHQF
HHHHHHHCCEEEEECCCCCCHHHHHEECCCCCCHHHHHCHHHHHHHHHHHHHHHCCCCCC
DHELFLKGQLTPVYFGTALGNFGVDHVLDGLVEWAPKPLSRAAKQRVVEPLEDVFSGFIF
CCEEEEECCCCEEEEEHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEE
KIQANMDPRHRDRIAFMRVCAGKYEKGMKMHHVRTGKMVSISDAVTFMAGDRTATEEAYP
EEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECHHEEEEECCCCCCCCCCC
GDIIGLHNHGTIQIGDTFTSGETLKFAGIPNFAPEMFRRIRLLDPLKQKQLLKGLMQLSE
CCEEEECCCCEEEECCEECCCCEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCC
EGAVQVFRPLRSNDLIVGAVGVLQFEVVVQRLKSEYKVDAIYEAISVATALWVESDNPLK
CCHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
MAEFEKKAYDNLALDGSNNLAYIAPTMVNLNLAMERYPDIRFRKTREH
HHHHHHHHHCCEEEECCCCEEEEEEEEEEEEEHHHHCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA