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The map label for this gene is rlmF

Identifier: 119944372

GI number: 119944372

Start: 781199

End: 782131

Strand: Direct

Name: rlmF

Synonym: Ping_0599

Alternate gene names: 119944372

Gene position: 781199-782131 (Clockwise)

Preceding gene: 119944371

Following gene: 119944378

Centisome position: 17.13

GC content: 39.12

Gene sequence:

>933_bases
ATGACCAAGGATATACAAGTTAAAAAATTATTACACCCAAGAAATTTACACCGGGGTCATTACGATTTAAAACAACTTTG
CGAGCAATCCCCATTATTAAGCACATTTTTAAGGACTAATCCTAAAGGTGAACAAACACTTGATTTTGCTGAGCCACAGG
CGGTATTACTGCTCAATCAAGTTTTGCTTAAACAGTTTTATCATGTTGATTTCTGGCAAATACCGAAGGGTTATTTATGC
CCGCCAATTCCGGGGCGTGTCGATTATATTCATTATCTTGCGGATTTATTGGGCGACACTTTTCATGGAAAAATCCCAGA
AGGTAAGCAGGTTAAGGTATTAGATATAGGGACAGGTGCAAATTGCATTTATCCTATTTTAGGCAGCCAAAGTTACGGAT
GGTCTTTTGTGGGAACAGATATTGATCCGTTATCGGTAAAAATGGCGGGGCTGATTATAAAATCAAATGTCTCTCTTAAA
CCTTTTATTAAAGTGCAGTTACAAGCGAATAAACAGGCTATTTTTGCAGGGATCATTAAACCAAAAGACAAATTTACCTT
AACCATGTGTAATCCCCCTTTTCATGCCTCTATGGAAAAAGCGCTTGCCGGAAGTGCACGAAAAATAAAGAATCTCAGTA
ACGATCAAAATAACCTATCGCGAGTACTTAATTTTGCAGGGCAGGAAGGTGAGCTTTGTTGTGCTGGTGGGGAAATCCGA
TTTTTAAAACAGATGATTCAGGAAAGTAAAGATTATGCCCGGCAGGTGTGCTGGTTTACCAGTTTAGTTTCAAAGTCCGA
CAATATAGCACCATTAAAGCAACAACTTGAAAAAGTGGGCGCTGAGCATGTAAAGGTTATTAAAATGGCGCAAGGGCAGA
AAGTGAGCCGCTTTATTGCCTGGAGTTTTTTACCCCAACCGCAAAATTTTTAG

Upstream 100 bases:

>100_bases
AACACTTATTTGATGCGTTTGGTATAAGCATCTCTTTAGATAATTTAGTCAATGACTTTAACATTATGCTTTTTAACCGC
CCATTTTTTGGATAAATTAA

Downstream 100 bases:

>100_bases
CAACAGGTTATTTTTTTTAAATGATCAAGCCGGAGAATCTGGCACTTTGATTACGACCTTAAAAAACCTGAATATCACAT
TCAGGTTTTTTAAGGGTCGA

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTKDIQVKKLLHPRNLHRGHYDLKQLCEQSPLLSTFLRTNPKGEQTLDFAEPQAVLLLNQVLLKQFYHVDFWQIPKGYLC
PPIPGRVDYIHYLADLLGDTFHGKIPEGKQVKVLDIGTGANCIYPILGSQSYGWSFVGTDIDPLSVKMAGLIIKSNVSLK
PFIKVQLQANKQAIFAGIIKPKDKFTLTMCNPPFHASMEKALAGSARKIKNLSNDQNNLSRVLNFAGQEGELCCAGGEIR
FLKQMIQESKDYARQVCWFTSLVSKSDNIAPLKQQLEKVGAEHVKVIKMAQGQKVSRFIAWSFLPQPQNF

Sequences:

>Translated_310_residues
MTKDIQVKKLLHPRNLHRGHYDLKQLCEQSPLLSTFLRTNPKGEQTLDFAEPQAVLLLNQVLLKQFYHVDFWQIPKGYLC
PPIPGRVDYIHYLADLLGDTFHGKIPEGKQVKVLDIGTGANCIYPILGSQSYGWSFVGTDIDPLSVKMAGLIIKSNVSLK
PFIKVQLQANKQAIFAGIIKPKDKFTLTMCNPPFHASMEKALAGSARKIKNLSNDQNNLSRVLNFAGQEGELCCAGGEIR
FLKQMIQESKDYARQVCWFTSLVSKSDNIAPLKQQLEKVGAEHVKVIKMAQGQKVSRFIAWSFLPQPQNF
>Mature_309_residues
TKDIQVKKLLHPRNLHRGHYDLKQLCEQSPLLSTFLRTNPKGEQTLDFAEPQAVLLLNQVLLKQFYHVDFWQIPKGYLCP
PIPGRVDYIHYLADLLGDTFHGKIPEGKQVKVLDIGTGANCIYPILGSQSYGWSFVGTDIDPLSVKMAGLIIKSNVSLKP
FIKVQLQANKQAIFAGIIKPKDKFTLTMCNPPFHASMEKALAGSARKIKNLSNDQNNLSRVLNFAGQEGELCCAGGEIRF
LKQMIQESKDYARQVCWFTSLVSKSDNIAPLKQQLEKVGAEHVKVIKMAQGQKVSRFIAWSFLPQPQNF

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family

Homologues:

Organism=Homo sapiens, GI122114654, Length=302, Percent_Identity=29.1390728476821, Blast_Score=143, Evalue=3e-34,
Organism=Escherichia coli, GI87081786, Length=300, Percent_Identity=51.6666666666667, Blast_Score=311, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI32564931, Length=308, Percent_Identity=28.2467532467532, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI32564933, Length=308, Percent_Identity=25.3246753246753, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI281363415, Length=301, Percent_Identity=25.5813953488372, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19922302, Length=301, Percent_Identity=25.5813953488372, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMF_PSYIN (A1SSI8)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942052.1
- ProteinModelPortal:   A1SSI8
- SMR:   A1SSI8
- STRING:   A1SSI8
- GeneID:   4624245
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0599
- eggNOG:   COG3129
- HOGENOM:   HBG747880
- OMA:   AELWCEG
- PhylomeDB:   A1SSI8
- ProtClustDB:   PRK11727
- BioCyc:   PING357804:PING_0599-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01848
- InterPro:   IPR016909
- InterPro:   IPR010286
- PANTHER:   PTHR13393
- PIRSF:   PIRSF029038

Pfam domain/function: PF05971 Methyltransf_10

EC number: =2.1.1.181

Molecular weight: Translated: 34853; Mature: 34722

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKDIQVKKLLHPRNLHRGHYDLKQLCEQSPLLSTFLRTNPKGEQTLDFAEPQAVLLLNQ
CCCCCCHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
VLLKQFYHVDFWQIPKGYLCPPIPGRVDYIHYLADLLGDTFHGKIPEGKQVKVLDIGTGA
HHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCC
NCIYPILGSQSYGWSFVGTDIDPLSVKMAGLIIKSNVSLKPFIKVQLQANKQAIFAGIIK
CEEEEECCCCCCCCEEECCCCCCHHHEEEEEEEECCCCCCCEEEEEEECCCEEEEEEEEC
PKDKFTLTMCNPPFHASMEKALAGSARKIKNLSNDQNNLSRVLNFAGQEGELCCAGGEIR
CCCCEEEEEECCCCHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCCHH
FLKQMIQESKDYARQVCWFTSLVSKSDNIAPLKQQLEKVGAEHVKVIKMAQGQKVSRFIA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHH
WSFLPQPQNF
HHCCCCCCCC
>Mature Secondary Structure 
TKDIQVKKLLHPRNLHRGHYDLKQLCEQSPLLSTFLRTNPKGEQTLDFAEPQAVLLLNQ
CCCCCHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
VLLKQFYHVDFWQIPKGYLCPPIPGRVDYIHYLADLLGDTFHGKIPEGKQVKVLDIGTGA
HHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCC
NCIYPILGSQSYGWSFVGTDIDPLSVKMAGLIIKSNVSLKPFIKVQLQANKQAIFAGIIK
CEEEEECCCCCCCCEEECCCCCCHHHEEEEEEEECCCCCCCEEEEEEECCCEEEEEEEEC
PKDKFTLTMCNPPFHASMEKALAGSARKIKNLSNDQNNLSRVLNFAGQEGELCCAGGEIR
CCCCEEEEEECCCCHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCCHH
FLKQMIQESKDYARQVCWFTSLVSKSDNIAPLKQQLEKVGAEHVKVIKMAQGQKVSRFIA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHH
WSFLPQPQNF
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA