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The map label for this gene is pntA [H]

Identifier: 119944339

GI number: 119944339

Start: 734448

End: 735992

Strand: Reverse

Name: pntA [H]

Synonym: Ping_0566

Alternate gene names: 119944339

Gene position: 735992-734448 (Counterclockwise)

Preceding gene: 119944340

Following gene: 119944338

Centisome position: 16.14

GC content: 45.5

Gene sequence:

>1545_bases
ATGCAGATAGGTATACCTAGAGAGATCCAAGAGAATGAAAACCGCGTGGCAGCAACACCAAATACTGTTGAGCTGCTCAT
TAAACAGGGTTTTACTTTTCTTGTGGAAAAAAACGCCGGAATAGCTGCCAGCTTTTCTGATCAAGCCTACCAGAATTCTG
GTGCCGAAATTGGCACTGATAAAAATCTGGTATGGCAATCAGATATTATTTTCAAAGTGAATGCGCCTACCGATACTGAG
ATTACCTTAATGAAGGAAGGGGCGACCCTAGTCAGTTTCATCTGGCCAGCACAAAACCCTGAATTACTTGAAAAATTAAC
CAGTAAAAAAATCAGTGTTATTGCAATGGATTCAGTACCACGTACTTCCCGGGCACAAGCACTTGATGCACTGAGCTCAA
TGGCCAATATCGCAGGTTACCGTGCCGTTATTGAAGCCGCTAATCAGTTTGGCCGTTTCTTCACCGGACAGATCACGGCA
GCAGGTAAAGTACCACCCGCCAAGGTACTGATTATAGGCGCGGGTGTTGCAGGTCTTTCTGCATTAGGCACCGCCGGCAG
TTTAGGCGCAATTGTACGCGCATTTGATACTCGCCCTGAAGTAAAAGAGCAAGTTGAGAGTATGGGTGCTGAATTCTTAG
TCGTTGAATACGAAGAAGAAAACGAGGTCAGCAGCTCGGGTTATGCTAAGACTATGAGCGAGGGTTATCAAAAAGCGCAA
GCTGCAATGATGGCTGAGCAAGCTAAAGATGTTGACATCATTATTACAACGGCACTTATTCCAGGTAAACCGGCTCCGCT
TCTTGTGACCAAAGAAATGATAGCATCGATGAAAGCGGGTAGTGTGATCGTTGATTTAGCAGCGCAAGCAGGCGGTAACG
CGGCACTCACGGTTAAAGATGAAGTTTTCTTAACCGATAACGGTGTAAAAATCGTTGGCTATACGGATCTTCCCAGCCGT
TTACCGACGCAATCAAGCCAGCTTTATTCAACCAATTTAGTTAACTTGATGAAGCTGCTTTGTAAAGATAAAGACGGCAT
TATCAATATTGACTTCAACGATGAAGTTATCCGCGGTGCAACCGTTGTCAAAGAAGGTGAAATTTCCTGGCCACCACCGG
CAATCAATGTCAGTGCTGCACCTGCGCCTAAAGCAGAAGCTGCAAAATCGGCTACCGTTGTTGAGAAAAAACCAGAAAAT
AAATGGATTAAACCCGCTGCAGCTGTTGTTGCTGCCATCCTATTTGGATGGCTTGCAAGCGTCGCACCGGCAAACTTTCT
CGCTCATTTTACCGTCTTTATACTGGCCTGTATTGTGGGTTACGGCGTGGTTTGGAATGTTGCCCATTCTCTGCATACAC
CACTAATGAGTGTAACCAACGCAATCAGCGGCATTATCATCATCGGAGCGCTGCTGCAGGTAGGTCAAGGTAACGGTTTT
GTGACTACCCTGTCGACCATTGCAATACTGATCGCCACTATCAATATCGTGGGTGGCTTTACCGTTACTCAGCGTATGTT
GAAAATGTTCCAAAAGGATAAATAA

Upstream 100 bases:

>100_bases
GCCTGTACAAATAAAAGCTGAATATGATCCAGATCTATTTATGCCTTTTTTTTGTACGTAAAATCAACAATTCATAATAA
CAAGCGATCATGGACTTACT

Downstream 100 bases:

>100_bases
GCATGTCTCAAGGATTAATTACAGCTGCATACATGATTGCAGCCATCCTCTTTATTGCCAGCCTTAACGGCTTAAGCAGT
CAAGAAAGTGCCAAGCGTGG

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MQIGIPREIQENENRVAATPNTVELLIKQGFTFLVEKNAGIAASFSDQAYQNSGAEIGTDKNLVWQSDIIFKVNAPTDTE
ITLMKEGATLVSFIWPAQNPELLEKLTSKKISVIAMDSVPRTSRAQALDALSSMANIAGYRAVIEAANQFGRFFTGQITA
AGKVPPAKVLIIGAGVAGLSALGTAGSLGAIVRAFDTRPEVKEQVESMGAEFLVVEYEEENEVSSSGYAKTMSEGYQKAQ
AAMMAEQAKDVDIIITTALIPGKPAPLLVTKEMIASMKAGSVIVDLAAQAGGNAALTVKDEVFLTDNGVKIVGYTDLPSR
LPTQSSQLYSTNLVNLMKLLCKDKDGIINIDFNDEVIRGATVVKEGEISWPPPAINVSAAPAPKAEAAKSATVVEKKPEN
KWIKPAAAVVAAILFGWLASVAPANFLAHFTVFILACIVGYGVVWNVAHSLHTPLMSVTNAISGIIIIGALLQVGQGNGF
VTTLSTIAILIATINIVGGFTVTQRMLKMFQKDK

Sequences:

>Translated_514_residues
MQIGIPREIQENENRVAATPNTVELLIKQGFTFLVEKNAGIAASFSDQAYQNSGAEIGTDKNLVWQSDIIFKVNAPTDTE
ITLMKEGATLVSFIWPAQNPELLEKLTSKKISVIAMDSVPRTSRAQALDALSSMANIAGYRAVIEAANQFGRFFTGQITA
AGKVPPAKVLIIGAGVAGLSALGTAGSLGAIVRAFDTRPEVKEQVESMGAEFLVVEYEEENEVSSSGYAKTMSEGYQKAQ
AAMMAEQAKDVDIIITTALIPGKPAPLLVTKEMIASMKAGSVIVDLAAQAGGNAALTVKDEVFLTDNGVKIVGYTDLPSR
LPTQSSQLYSTNLVNLMKLLCKDKDGIINIDFNDEVIRGATVVKEGEISWPPPAINVSAAPAPKAEAAKSATVVEKKPEN
KWIKPAAAVVAAILFGWLASVAPANFLAHFTVFILACIVGYGVVWNVAHSLHTPLMSVTNAISGIIIIGALLQVGQGNGF
VTTLSTIAILIATINIVGGFTVTQRMLKMFQKDK
>Mature_514_residues
MQIGIPREIQENENRVAATPNTVELLIKQGFTFLVEKNAGIAASFSDQAYQNSGAEIGTDKNLVWQSDIIFKVNAPTDTE
ITLMKEGATLVSFIWPAQNPELLEKLTSKKISVIAMDSVPRTSRAQALDALSSMANIAGYRAVIEAANQFGRFFTGQITA
AGKVPPAKVLIIGAGVAGLSALGTAGSLGAIVRAFDTRPEVKEQVESMGAEFLVVEYEEENEVSSSGYAKTMSEGYQKAQ
AAMMAEQAKDVDIIITTALIPGKPAPLLVTKEMIASMKAGSVIVDLAAQAGGNAALTVKDEVFLTDNGVKIVGYTDLPSR
LPTQSSQLYSTNLVNLMKLLCKDKDGIINIDFNDEVIRGATVVKEGEISWPPPAINVSAAPAPKAEAAKSATVVEKKPEN
KWIKPAAAVVAAILFGWLASVAPANFLAHFTVFILACIVGYGVVWNVAHSLHTPLMSVTNAISGIIIIGALLQVGQGNGF
VTTLSTIAILIATINIVGGFTVTQRMLKMFQKDK

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=539, Percent_Identity=50.0927643784787, Blast_Score=469, Evalue=1e-132,
Organism=Homo sapiens, GI122939153, Length=539, Percent_Identity=50.0927643784787, Blast_Score=469, Evalue=1e-132,
Organism=Escherichia coli, GI1787887, Length=513, Percent_Identity=69.0058479532164, Blast_Score=709, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=520, Percent_Identity=52.5, Blast_Score=496, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 54524; Mature: 54524

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00836 ALADH_PNT_1 ; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGIPREIQENENRVAATPNTVELLIKQGFTFLVEKNAGIAASFSDQAYQNSGAEIGTD
CCCCCCCHHHCCCCEEEECCHHHHHHHHCCCEEEEECCCCEEECCCCHHHHCCCCCCCCC
KNLVWQSDIIFKVNAPTDTEITLMKEGATLVSFIWPAQNPELLEKLTSKKISVIAMDSVP
CCEEEECCEEEEECCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHCCCEEEEEECCCC
RTSRAQALDALSSMANIAGYRAVIEAANQFGRFFTGQITAAGKVPPAKVLIIGAGVAGLS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECEEEECCCCCCCEEEEEECCHHHHH
ALGTAGSLGAIVRAFDTRPEVKEQVESMGAEFLVVEYEEENEVSSSGYAKTMSEGYQKAQ
HHCCCCHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHH
AAMMAEQAKDVDIIITTALIPGKPAPLLVTKEMIASMKAGSVIVDLAAQAGGNAALTVKD
HHHHHHHCCCCEEEEEEEECCCCCCCEEEEHHHHHHHCCCCEEEEEHHCCCCCEEEEEEC
EVFLTDNGVKIVGYTDLPSRLPTQSSQLYSTNLVNLMKLLCKDKDGIINIDFNDEVIRGA
EEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHCCC
TVVKEGEISWPPPAINVSAAPAPKAEAAKSATVVEKKPENKWIKPAAAVVAAILFGWLAS
EEEECCCCCCCCCCCCCCCCCCCCHHHHCCCHHEECCCCCCCCCHHHHHHHHHHHHHHHH
VAPANFLAHFTVFILACIVGYGVVWNVAHSLHTPLMSVTNAISGIIIIGALLQVGQGNGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VTTLSTIAILIATINIVGGFTVTQRMLKMFQKDK
EHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQIGIPREIQENENRVAATPNTVELLIKQGFTFLVEKNAGIAASFSDQAYQNSGAEIGTD
CCCCCCCHHHCCCCEEEECCHHHHHHHHCCCEEEEECCCCEEECCCCHHHHCCCCCCCCC
KNLVWQSDIIFKVNAPTDTEITLMKEGATLVSFIWPAQNPELLEKLTSKKISVIAMDSVP
CCEEEECCEEEEECCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHCCCEEEEEECCCC
RTSRAQALDALSSMANIAGYRAVIEAANQFGRFFTGQITAAGKVPPAKVLIIGAGVAGLS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECEEEECCCCCCCEEEEEECCHHHHH
ALGTAGSLGAIVRAFDTRPEVKEQVESMGAEFLVVEYEEENEVSSSGYAKTMSEGYQKAQ
HHCCCCHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHH
AAMMAEQAKDVDIIITTALIPGKPAPLLVTKEMIASMKAGSVIVDLAAQAGGNAALTVKD
HHHHHHHCCCCEEEEEEEECCCCCCCEEEEHHHHHHHCCCCEEEEEHHCCCCCEEEEEEC
EVFLTDNGVKIVGYTDLPSRLPTQSSQLYSTNLVNLMKLLCKDKDGIINIDFNDEVIRGA
EEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHCCC
TVVKEGEISWPPPAINVSAAPAPKAEAAKSATVVEKKPENKWIKPAAAVVAAILFGWLAS
EEEECCCCCCCCCCCCCCCCCCCCHHHHCCCHHEECCCCCCCCCHHHHHHHHHHHHHHHH
VAPANFLAHFTVFILACIVGYGVVWNVAHSLHTPLMSVTNAISGIIIIGALLQVGQGNGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VTTLSTIAILIATINIVGGFTVTQRMLKMFQKDK
EHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]