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The map label for this gene is pntB [H]

Identifier: 119944338

GI number: 119944338

Start: 733069

End: 734445

Strand: Reverse

Name: pntB [H]

Synonym: Ping_0565

Alternate gene names: 119944338

Gene position: 734445-733069 (Counterclockwise)

Preceding gene: 119944339

Following gene: 119944333

Centisome position: 16.11

GC content: 44.08

Gene sequence:

>1377_bases
ATGTCTCAAGGATTAATTACAGCTGCATACATGATTGCAGCCATCCTCTTTATTGCCAGCCTTAACGGCTTAAGCAGTCA
AGAAAGTGCCAAGCGTGGTAACACTTTTGGCATCATAGGTATGATAGTTGCAATTATTGCAACTATCGCCAGCGCACAAG
TTGCAGGCATGGGTTACATTACCGTGGCAATGGCTATTGGCGCAGGGATTGGAGTTCGTTTAGCATTAAAAGTAGAAATG
ACTTCAATGCCGGAGCTTATCGCAGTACTGCATAGTTTTGTTGGTCTGGCTGCGGTCTTTATCGGTTTCAACTCTTATTT
AGAACATGGTCTGCTGACCGGTATTGAGCAATCAATCCATGATGTAGAAGTGTTTTTAGGTATCTTTATTGGCTCTGTTA
CCTTTACTGGTTCAATTGTTGCCTATGGTAAACTGAGCGGAAAAATTAACAGCACGCCACTTAACCTGCCGGGTAAAAAC
TGGATTAACCTTGCGGTGCTGGCATCAAGCGCCTTCCTGTTAGTGGGTTTTGTCAGCAGTGGTTTAGGTTTAACCGCTCT
TATCATAATGACCATTATTGCTCTGGCCTTCGGTTGGCATTTGGTTGCTTCAATCGGCGGCGCAGATATGCCGGTGGTTG
TATCAATGCTTAACTCCTATTCAGGTTGGGCAGCAGCAGCAACAGGTTTTATGCTCTCCAACGATCTGCTGATCATCACA
GGTGCATTAGTGGGTTCTTCAGGTGCCATTCTTTCTTATATTATGTGTAAAGCAATGAACCGTTCGTTCATTAGCGTTAT
CTTAGGTGGTTTTGGTAACGAAGTGACGGTGCAAAGTGGCGATGAGGAGCAGGGAGAGCATTTTGAAACAAGTGCTCAAG
AGGTTGCAGATTCACTGGCCGCGGCTAAAAATGTGGTAATTGTTCCAGGTTACGGCATGGCCGTTGCGCAGGCACAATAC
CCCGTTGCTGAACTAACCAAATACTTGCGCAGTAAAGGTAAAAAAGTGCGTTTTGGTATTCATCCGGTTGCAGGACGTCT
GCCTGGTCATATGAACGTATTATTAGCGGAAGCAAAAGTACCCTACGATATCGTATTGGAAATGGATGAAATCAATGACG
ATTTCGCTAAAACCGATGTGGTCTTAGTCATTGGTGCAAATGATACGGTCAATCCGGCCGCCAATGAGCCAGGTACGCCT
ATCTCAGGTATGCCAGTATTACACGTTTGGGAGGCCGAAAAAGTGGTTGTCTTTAAACGTTCAATGGCATCAGGTTATGC
AGGTGTGCAAAATCCATTGTTCTTTAAAGAGAACTCTGAAATGTTATTTGGCGATGCAAAAGCATCGGTTAATGCTATTT
TACAAAATCTTAATTAA

Upstream 100 bases:

>100_bases
CCCTGTCGACCATTGCAATACTGATCGCCACTATCAATATCGTGGGTGGCTTTACCGTTACTCAGCGTATGTTGAAAATG
TTCCAAAAGGATAAATAAGC

Downstream 100 bases:

>100_bases
CCTCTCAGTTAAATAAGATATTAATCAAAAAGGCGCTATCCAGCGCCTTTTTTGTATCTAAAAATCGGAAAGCTGGAAAG
CTAACGGCTGACGGCTGACG

Product: pyridine nucleotide transhydrogenase

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Pyridine nucleotide transhydrogenase subunit beta [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSQGLITAAYMIAAILFIASLNGLSSQESAKRGNTFGIIGMIVAIIATIASAQVAGMGYITVAMAIGAGIGVRLALKVEM
TSMPELIAVLHSFVGLAAVFIGFNSYLEHGLLTGIEQSIHDVEVFLGIFIGSVTFTGSIVAYGKLSGKINSTPLNLPGKN
WINLAVLASSAFLLVGFVSSGLGLTALIIMTIIALAFGWHLVASIGGADMPVVVSMLNSYSGWAAAATGFMLSNDLLIIT
GALVGSSGAILSYIMCKAMNRSFISVILGGFGNEVTVQSGDEEQGEHFETSAQEVADSLAAAKNVVIVPGYGMAVAQAQY
PVAELTKYLRSKGKKVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFAKTDVVLVIGANDTVNPAANEPGTP
ISGMPVLHVWEAEKVVVFKRSMASGYAGVQNPLFFKENSEMLFGDAKASVNAILQNLN

Sequences:

>Translated_458_residues
MSQGLITAAYMIAAILFIASLNGLSSQESAKRGNTFGIIGMIVAIIATIASAQVAGMGYITVAMAIGAGIGVRLALKVEM
TSMPELIAVLHSFVGLAAVFIGFNSYLEHGLLTGIEQSIHDVEVFLGIFIGSVTFTGSIVAYGKLSGKINSTPLNLPGKN
WINLAVLASSAFLLVGFVSSGLGLTALIIMTIIALAFGWHLVASIGGADMPVVVSMLNSYSGWAAAATGFMLSNDLLIIT
GALVGSSGAILSYIMCKAMNRSFISVILGGFGNEVTVQSGDEEQGEHFETSAQEVADSLAAAKNVVIVPGYGMAVAQAQY
PVAELTKYLRSKGKKVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFAKTDVVLVIGANDTVNPAANEPGTP
ISGMPVLHVWEAEKVVVFKRSMASGYAGVQNPLFFKENSEMLFGDAKASVNAILQNLN
>Mature_457_residues
SQGLITAAYMIAAILFIASLNGLSSQESAKRGNTFGIIGMIVAIIATIASAQVAGMGYITVAMAIGAGIGVRLALKVEMT
SMPELIAVLHSFVGLAAVFIGFNSYLEHGLLTGIEQSIHDVEVFLGIFIGSVTFTGSIVAYGKLSGKINSTPLNLPGKNW
INLAVLASSAFLLVGFVSSGLGLTALIIMTIIALAFGWHLVASIGGADMPVVVSMLNSYSGWAAAATGFMLSNDLLIITG
ALVGSSGAILSYIMCKAMNRSFISVILGGFGNEVTVQSGDEEQGEHFETSAQEVADSLAAAKNVVIVPGYGMAVAQAQYP
VAELTKYLRSKGKKVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFAKTDVVLVIGANDTVNPAANEPGTPI
SGMPVLHVWEAEKVVVFKRSMASGYAGVQNPLFFKENSEMLFGDAKASVNAILQNLN

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=454, Percent_Identity=49.3392070484581, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI122939153, Length=454, Percent_Identity=49.3392070484581, Blast_Score=429, Evalue=1e-120,
Organism=Escherichia coli, GI1787886, Length=462, Percent_Identity=71.2121212121212, Blast_Score=650, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=464, Percent_Identity=47.6293103448276, Blast_Score=397, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 47928; Mature: 47796

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQGLITAAYMIAAILFIASLNGLSSQESAKRGNTFGIIGMIVAIIATIASAQVAGMGYI
CCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCHHH
TVAMAIGAGIGVRLALKVEMTSMPELIAVLHSFVGLAAVFIGFNSYLEHGLLTGIEQSIH
HHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVEVFLGIFIGSVTFTGSIVAYGKLSGKINSTPLNLPGKNWINLAVLASSAFLLVGFVSS
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCEEHEEHHHHHHHHHHHHHC
GLGLTALIIMTIIALAFGWHLVASIGGADMPVVVSMLNSYSGWAAAATGFMLSNDLLIIT
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHEEECCEEEEE
GALVGSSGAILSYIMCKAMNRSFISVILGGFGNEVTVQSGDEEQGEHFETSAQEVADSLA
EEHHCCCCHHHHHHHHHHHCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHH
AAKNVVIVPGYGMAVAQAQYPVAELTKYLRSKGKKVRFGIHPVAGRLPGHMNVLLAEAKV
HHCCEEEECCCCCCHHHCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCC
PYDIVLEMDEINDDFAKTDVVLVIGANDTVNPAANEPGTPISGMPVLHVWEAEKVVVFKR
CEEEEEEEHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEH
SMASGYAGVQNPLFFKENSEMLFGDAKASVNAILQNLN
HHHCCCCCCCCCEEEECCCCEEEECHHHHHHHHHHCCC
>Mature Secondary Structure 
SQGLITAAYMIAAILFIASLNGLSSQESAKRGNTFGIIGMIVAIIATIASAQVAGMGYI
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCHHH
TVAMAIGAGIGVRLALKVEMTSMPELIAVLHSFVGLAAVFIGFNSYLEHGLLTGIEQSIH
HHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVEVFLGIFIGSVTFTGSIVAYGKLSGKINSTPLNLPGKNWINLAVLASSAFLLVGFVSS
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCEEHEEHHHHHHHHHHHHHC
GLGLTALIIMTIIALAFGWHLVASIGGADMPVVVSMLNSYSGWAAAATGFMLSNDLLIIT
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHEEECCEEEEE
GALVGSSGAILSYIMCKAMNRSFISVILGGFGNEVTVQSGDEEQGEHFETSAQEVADSLA
EEHHCCCCHHHHHHHHHHHCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHH
AAKNVVIVPGYGMAVAQAQYPVAELTKYLRSKGKKVRFGIHPVAGRLPGHMNVLLAEAKV
HHCCEEEECCCCCCHHHCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCC
PYDIVLEMDEINDDFAKTDVVLVIGANDTVNPAANEPGTPISGMPVLHVWEAEKVVVFKR
CEEEEEEEHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEH
SMASGYAGVQNPLFFKENSEMLFGDAKASVNAILQNLN
HHHCCCCCCCCCEEEECCCCEEEECHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]