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The map label for this gene is mscS [H]

Identifier: 119944314

GI number: 119944314

Start: 691807

End: 692628

Strand: Reverse

Name: mscS [H]

Synonym: Ping_0541

Alternate gene names: 119944314

Gene position: 692628-691807 (Counterclockwise)

Preceding gene: 119944316

Following gene: 119944313

Centisome position: 15.19

GC content: 41.12

Gene sequence:

>822_bases
ATGATGACTCCCGATCAAATGATCACCTTAGTAACAGTGAACGCTATCGAATACGGACCTAAGTTAATTGCCGCCATTGT
CCTTTGGGTGATTGGTAGTTGGGTCATTAAGTTGCTGGTTTCCGGTTTAAATAAAGCAATGGAAAAAGGCAAAGTCGACC
CTTCTTTAAAACCTTTTTTATGCAGCATCAGCGGCATAGTGCTTAAAGTGATGTTAGGTATAACTGTGCTTGGTATGCTG
AGTATTGAAATGACCTCTTTTGTTGCCATTCTTGCCGCAGCGGGTTTAGCTGTCGGTATGGCACTTTCAGGTACATTACA
AAACTTTGCCGGTGGTGTAATGATTCTGCTCTTTAAGCCCTTTAAAGTCGGAGACTTTATTGATGCACAGGGGCATATGG
GAACAGTTAAAGAAATACAAATTTTCAATAGCATTTTAACCACTCCCGATAACAAAACCATTATTATACCTAATGGCGGA
TTATCAACGAGCTCTATGGTGAACTTTTCAACTGAAAAAAAACGTCGCGTGGATTGGACTATCAGTGTTGCCTATGGTGA
TGACTTAGATAAAGCACGTGCAGTCATCAAACAACTTTGTGATGCAGATCAGCGTATATTCACAGATCCTGCCGTAGTTA
TTGTGGTTTCTGCACTAGCAGACAGTTCAGTGAATTTTTCGGTACGCGCCTGGGTTAACGCACCTGATTTTTTTCCGGTG
TTCTGGGCCATGAATGAAAATATCTATAAGACATTTCCCAAAGAAGGGCTTAATATTCCGTTCCCGCAAATGGATGTCCA
TCTGCATAAAGAATCTGCATAA

Upstream 100 bases:

>100_bases
TTCCAAATTATATAAAGTTTAACAGCTTAGTTCTAAGCAATACAAAACCGATATAATGTAGTCCTATTAATTGATAACAA
ATAACAAAGGTAAAAAATAA

Downstream 100 bases:

>100_bases
ATAAAGTCAGATTTTGCCTAAAGCCATCGTTATCACTTTGGCTTTAATCTCTTCTCCCCCAGCCTTAAAACAGCCCCTTT
TACTACTCTCAGAAAATAGT

Product: MscS mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MMTPDQMITLVTVNAIEYGPKLIAAIVLWVIGSWVIKLLVSGLNKAMEKGKVDPSLKPFLCSISGIVLKVMLGITVLGML
SIEMTSFVAILAAAGLAVGMALSGTLQNFAGGVMILLFKPFKVGDFIDAQGHMGTVKEIQIFNSILTTPDNKTIIIPNGG
LSTSSMVNFSTEKKRRVDWTISVAYGDDLDKARAVIKQLCDADQRIFTDPAVVIVVSALADSSVNFSVRAWVNAPDFFPV
FWAMNENIYKTFPKEGLNIPFPQMDVHLHKESA

Sequences:

>Translated_273_residues
MMTPDQMITLVTVNAIEYGPKLIAAIVLWVIGSWVIKLLVSGLNKAMEKGKVDPSLKPFLCSISGIVLKVMLGITVLGML
SIEMTSFVAILAAAGLAVGMALSGTLQNFAGGVMILLFKPFKVGDFIDAQGHMGTVKEIQIFNSILTTPDNKTIIIPNGG
LSTSSMVNFSTEKKRRVDWTISVAYGDDLDKARAVIKQLCDADQRIFTDPAVVIVVSALADSSVNFSVRAWVNAPDFFPV
FWAMNENIYKTFPKEGLNIPFPQMDVHLHKESA
>Mature_273_residues
MMTPDQMITLVTVNAIEYGPKLIAAIVLWVIGSWVIKLLVSGLNKAMEKGKVDPSLKPFLCSISGIVLKVMLGITVLGML
SIEMTSFVAILAAAGLAVGMALSGTLQNFAGGVMILLFKPFKVGDFIDAQGHMGTVKEIQIFNSILTTPDNKTIIIPNGG
LSTSSMVNFSTEKKRRVDWTISVAYGDDLDKARAVIKQLCDADQRIFTDPAVVIVVSALADSSVNFSVRAWVNAPDFFPV
FWAMNENIYKTFPKEGLNIPFPQMDVHLHKESA

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=255, Percent_Identity=34.5098039215686, Blast_Score=196, Evalue=9e-52,
Organism=Escherichia coli, GI1786670, Length=276, Percent_Identity=26.4492753623188, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI2367355, Length=260, Percent_Identity=23.0769230769231, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787591, Length=243, Percent_Identity=25.5144032921811, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 29669; Mature: 29669

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTPDQMITLVTVNAIEYGPKLIAAIVLWVIGSWVIKLLVSGLNKAMEKGKVDPSLKPFL
CCCCHHEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
CSISGIVLKVMLGITVLGMLSIEMTSFVAILAAAGLAVGMALSGTLQNFAGGVMILLFKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
FKVGDFIDAQGHMGTVKEIQIFNSILTTPDNKTIIIPNGGLSTSSMVNFSTEKKRRVDWT
CCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCCCEEEEEE
ISVAYGDDLDKARAVIKQLCDADQRIFTDPAVVIVVSALADSSVNFSVRAWVNAPDFFPV
EEEEECCCHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCEEE
FWAMNENIYKTFPKEGLNIPFPQMDVHLHKESA
EEECCCCHHHHCCCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MMTPDQMITLVTVNAIEYGPKLIAAIVLWVIGSWVIKLLVSGLNKAMEKGKVDPSLKPFL
CCCCHHEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
CSISGIVLKVMLGITVLGMLSIEMTSFVAILAAAGLAVGMALSGTLQNFAGGVMILLFKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
FKVGDFIDAQGHMGTVKEIQIFNSILTTPDNKTIIIPNGGLSTSSMVNFSTEKKRRVDWT
CCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCCCEEEEEE
ISVAYGDDLDKARAVIKQLCDADQRIFTDPAVVIVVSALADSSVNFSVRAWVNAPDFFPV
EEEEECCCHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCEEE
FWAMNENIYKTFPKEGLNIPFPQMDVHLHKESA
EEECCCCHHHHCCCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]