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The map label for this gene is ispB [H]

Identifier: 119944297

GI number: 119944297

Start: 668382

End: 669377

Strand: Reverse

Name: ispB [H]

Synonym: Ping_0524

Alternate gene names: 119944297

Gene position: 669377-668382 (Counterclockwise)

Preceding gene: 119944301

Following gene: 119944290

Centisome position: 14.68

GC content: 38.55

Gene sequence:

>996_bases
ATGGCTATTTGGACTTTTAGGACCATGGATATAAAAACAATTCAGCGTATTTCTGCAACTGAAATGAATCAGGTCAATAA
ACTGATCACGGAACAATTAAGCTCGGATGTCGCCTTAATTAATCAACTGGGTTTTTATATTATTAATAGCGGTGGTAAAC
GTATCCGCCCGTTAATTACCGTATTAGCAGCAAAAGCCCTGGGTATTCATGATCAAAATACGACCAAACTTGCCGCGATA
ATTGAATTTATTCATACGGCAACCTTGCTGCACGATGATGTCGTTGATGACTCAGATCTGCGCCGCGGCAAAAAAACAGC
GAATGCGGTATTTGGTTCAGCAGCAAGCGTATTAGTCGGGGATTTTTTATATACCCGCTCTTTTCAGATGATGACGCAGT
TGCGTAATTTAAAAGTGATGGATATTTTATCTTCAGCAACCAATATTATTGCTGAAGGTGAAGTTTTACAGTTAATGAAT
TGTAATGATCCGGACACCACTGAAAAAAAATATATGGATATTATCTACTTTAAAACTGCCAAACTATTTGAAGCGGCAAC
ACAACTTGTCGCCGTATTAGGAGAGCAATCTGAGGAAATTGAAACAGCTTTACTCAATTATGGTAAATACCTTGGGACGG
CATTCCAGTTAACCGATGATGTGATGGATTATAACTCTGACGCAAGCGAAATGGGTAAAAATACAGGAGATGACCTGGCA
GAAGGCAAACCGACCTTACCGCTTTTATATGCGATTCAACACGGCAATAAAAAGCAAGCAACGCTTATCAAAGAGGCCAT
TAAAAAAGGTAATGGCATGGATCATTTTGATGAGATTATGCAGGCACTGCATGATACAAATGCATTAGAATATGCAAAAA
AACGCGCACTTGAAGAAGCAGATAAAGCCATTAAAGCTCTTGTTATTATTCCAGACAGTGAATACAAACAAGCATTAATA
AGCTTGGCTCATATTGCTGCAGATCGAAAATCTTAA

Upstream 100 bases:

>100_bases
ATGTGCAATAGTGCACTGGATTATGAAAAGCAGGATAATTTTTAGCTTAAGCAGTTGAGATATCACTAATAAACGGCAAT
AATAGCGACTCTTTTTAAGA

Downstream 100 bases:

>100_bases
AAAGGCTTTAGCAGAGCACTGTTAAGTAGATACAAACAAGCATTAATAAGCTTGTCTCATATTGCTGCAGATCGAAAATC
TTAAAAAAGCTTTAGCAGAG

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MAIWTFRTMDIKTIQRISATEMNQVNKLITEQLSSDVALINQLGFYIINSGGKRIRPLITVLAAKALGIHDQNTTKLAAI
IEFIHTATLLHDDVVDDSDLRRGKKTANAVFGSAASVLVGDFLYTRSFQMMTQLRNLKVMDILSSATNIIAEGEVLQLMN
CNDPDTTEKKYMDIIYFKTAKLFEAATQLVAVLGEQSEEIETALLNYGKYLGTAFQLTDDVMDYNSDASEMGKNTGDDLA
EGKPTLPLLYAIQHGNKKQATLIKEAIKKGNGMDHFDEIMQALHDTNALEYAKKRALEEADKAIKALVIIPDSEYKQALI
SLAHIAADRKS

Sequences:

>Translated_331_residues
MAIWTFRTMDIKTIQRISATEMNQVNKLITEQLSSDVALINQLGFYIINSGGKRIRPLITVLAAKALGIHDQNTTKLAAI
IEFIHTATLLHDDVVDDSDLRRGKKTANAVFGSAASVLVGDFLYTRSFQMMTQLRNLKVMDILSSATNIIAEGEVLQLMN
CNDPDTTEKKYMDIIYFKTAKLFEAATQLVAVLGEQSEEIETALLNYGKYLGTAFQLTDDVMDYNSDASEMGKNTGDDLA
EGKPTLPLLYAIQHGNKKQATLIKEAIKKGNGMDHFDEIMQALHDTNALEYAKKRALEEADKAIKALVIIPDSEYKQALI
SLAHIAADRKS
>Mature_330_residues
AIWTFRTMDIKTIQRISATEMNQVNKLITEQLSSDVALINQLGFYIINSGGKRIRPLITVLAAKALGIHDQNTTKLAAII
EFIHTATLLHDDVVDDSDLRRGKKTANAVFGSAASVLVGDFLYTRSFQMMTQLRNLKVMDILSSATNIIAEGEVLQLMNC
NDPDTTEKKYMDIIYFKTAKLFEAATQLVAVLGEQSEEIETALLNYGKYLGTAFQLTDDVMDYNSDASEMGKNTGDDLAE
GKPTLPLLYAIQHGNKKQATLIKEAIKKGNGMDHFDEIMQALHDTNALEYAKKRALEEADKAIKALVIIPDSEYKQALIS
LAHIAADRKS

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=308, Percent_Identity=31.1688311688312, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI4758430, Length=266, Percent_Identity=24.4360902255639, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI83700220, Length=266, Percent_Identity=24.4360902255639, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=59.5015576323988, Blast_Score=413, Evalue=1e-117,
Organism=Escherichia coli, GI1786623, Length=259, Percent_Identity=30.8880308880309, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17505681, Length=349, Percent_Identity=28.9398280802292, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319475, Length=339, Percent_Identity=30.3834808259587, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6325188, Length=277, Percent_Identity=23.826714801444, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24651612, Length=298, Percent_Identity=28.5234899328859, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI281365769, Length=215, Percent_Identity=24.1860465116279, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24660002, Length=215, Percent_Identity=24.1860465116279, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36668; Mature: 36536

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIWTFRTMDIKTIQRISATEMNQVNKLITEQLSSDVALINQLGFYIINSGGKRIRPLIT
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHH
VLAAKALGIHDQNTTKLAAIIEFIHTATLLHDDVVDDSDLRRGKKTANAVFGSAASVLVG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
DFLYTRSFQMMTQLRNLKVMDILSSATNIIAEGEVLQLMNCNDPDTTEKKYMDIIYFKTA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
KLFEAATQLVAVLGEQSEEIETALLNYGKYLGTAFQLTDDVMDYNSDASEMGKNTGDDLA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHH
EGKPTLPLLYAIQHGNKKQATLIKEAIKKGNGMDHFDEIMQALHDTNALEYAKKRALEEA
CCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
DKAIKALVIIPDSEYKQALISLAHIAADRKS
HHHHEEEEEECCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AIWTFRTMDIKTIQRISATEMNQVNKLITEQLSSDVALINQLGFYIINSGGKRIRPLIT
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHH
VLAAKALGIHDQNTTKLAAIIEFIHTATLLHDDVVDDSDLRRGKKTANAVFGSAASVLVG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
DFLYTRSFQMMTQLRNLKVMDILSSATNIIAEGEVLQLMNCNDPDTTEKKYMDIIYFKTA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
KLFEAATQLVAVLGEQSEEIETALLNYGKYLGTAFQLTDDVMDYNSDASEMGKNTGDDLA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHH
EGKPTLPLLYAIQHGNKKQATLIKEAIKKGNGMDHFDEIMQALHDTNALEYAKKRALEEA
CCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
DKAIKALVIIPDSEYKQALISLAHIAADRKS
HHHHEEEEEECCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]