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The map label for this gene is dnaG [H]

Identifier: 119944096

GI number: 119944096

Start: 411412

End: 413163

Strand: Reverse

Name: dnaG [H]

Synonym: Ping_0311

Alternate gene names: 119944096

Gene position: 413163-411412 (Counterclockwise)

Preceding gene: 119944097

Following gene: 119944095

Centisome position: 9.06

GC content: 40.24

Gene sequence:

>1752_bases
ATGGCAGGACGTATTCCAAGAAATTTTATTGACGATCTTATTGCTCGTACTGATATTGTTGATTTTATTGATAGCCGTGT
AAAACTAAAAAAACAGGGTAAAGATTACCGCGCTTGTTGTCCTTTCCATAATGGTAATAATAACTCATCATTTTCAGTCA
GTTCAGACAAACAGTTCTATCATTGCTTTAACTGTGGTGTGAGCGGAAATGTACTCTCTTTTATTATGGAATATGACGGC
ATTGAATTTGTTGATGCCATCGAAACTCTGGCAGAGCAGTTATCGATAGAAGTCCCTCGCGAAGAAAGCGGCTTTGGAAA
AGCACACCAAAGCAGCTATGTAGACCGCAAAGATCTGTATCAGTTAATGGCTGATATTGCTGTTTTTTATCAGCAGCAAC
TGCGCACGCACAAAAACAGTCAACAGGTTATTAATTACCTTAAGGGTCGCGGATTATCGGGGCAGACAGTTAAGCATTTT
AAGATAGGTTATGCACCCGATGGTTGGAATGAAGTGCGCAAACGATTCGCAGTGTCTGCCGATTTAGAAAAACAGTTGAT
TGAAGCAGGCATGTTAATAAGCAAAGAAAAAAGTGGCAGTTATGACCGTTTCCGCGATCGACTGATGTTCCCTATCCACG
ATAGGCGTGGCCGAGTTATTGGTTTTGGAGGTCGTGTTTTAGGTGATAATGAGCCTAAGTACCTGAATTCTCCCGAAACA
CCTCTCTTTCACAAGGGCAAAGAGCTCTACGGTTTATACCAGGTTAAGCAAGCCTATAAAGAAATTAAACGCGTATTAGT
GGTTGAAGGTTATATGGATGTTGTGGCACTTGGTCAATTTGGTATTGATTATGCGGTGGCATCCCTGGGTACCAGTACGA
CGCCAGAGCATATCCAACAGCTCTTCCGCCACACCAGCGAAGTGATTTGTTGTTTTGATGGTGATAAAGCGGGTAAAGCC
GCTGCGTGGCGAGCATTAGAAAATGCGCTCCCCTATATACAAGATGGCCGAACTTTACGTTTTATGTTTCTTGCTGATGG
TGAAGATCCTGATAGTTACGTACAAAAAGAGGGTAAAGAAGCCTTTGAAAAACTGATTGAACAGGCATTACCTTTATCTG
ATTTTCTTTTTCAGCATTTATTAAATCAAGTTGATATGAGCACTGCTGACAGCAAAGCTAAACTGGCGCAATTAGCAATC
CCATTAATGAGTAAACTGCCCGACACGGTATTCAGGCAAATGATGGAGGGAAGGCTTATTGCGTTATTGGGGATTGAAAA
AGAGGGACTCAATAAATTATTACCCGCAGCAGCATCAAACAGTAGAATAGATAAAAGACAAAAAGTAACCCCCATGCGTT
TAGCTATTTCGGTGCTTTTACAGCATCCCAACATTGCCTATGGTTTGCCTGAATTTCCTGAATTTAAGGAAATGACCTTA
CCTGGCTTAAGTTTATTAAATTCGTTACTTGAAATTTGCCGTACTACCCCCACTATAACAACAGGGCAATTACTGGAACA
TTGGCGAGAAAAGCCAGAAAGTAGTCAACTGAATAAATTAGCCGTGTGGCAAAACGATATAAAAGAAGATTATTATGAAG
CTTTTTTCCTAGACACGTTAGAAAATTTTCTTAATTTTCATCTTACAAGTAAAATAGAGTTTTTAAAACAAAAATCTCGT
TTAGGCCAAGCGCTAACAAGTGCAGAAACACAGCAACTTGCATTACTGCTTGCTGAACAAAAACAGCACTAA

Upstream 100 bases:

>100_bases
TCCGGGCAGAGCGATTTAGAATGTCTATTCCCTTATAAAGTGCTATAAAGGAATAGCCAAAACGGCTTTTTTTAATTAAT
AGACTAAATGGTTTAAAGAC

Downstream 100 bases:

>100_bases
TGAACTAGAAAACTTCAGAAAATTAGCGTATACTAGATCGCTTGTACTTTTTTTATATAACCCCTATTTGCAATTGATTT
TTGATTTGCAATCAATTTTT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 583; Mature: 582

Protein sequence:

>583_residues
MAGRIPRNFIDDLIARTDIVDFIDSRVKLKKQGKDYRACCPFHNGNNNSSFSVSSDKQFYHCFNCGVSGNVLSFIMEYDG
IEFVDAIETLAEQLSIEVPREESGFGKAHQSSYVDRKDLYQLMADIAVFYQQQLRTHKNSQQVINYLKGRGLSGQTVKHF
KIGYAPDGWNEVRKRFAVSADLEKQLIEAGMLISKEKSGSYDRFRDRLMFPIHDRRGRVIGFGGRVLGDNEPKYLNSPET
PLFHKGKELYGLYQVKQAYKEIKRVLVVEGYMDVVALGQFGIDYAVASLGTSTTPEHIQQLFRHTSEVICCFDGDKAGKA
AAWRALENALPYIQDGRTLRFMFLADGEDPDSYVQKEGKEAFEKLIEQALPLSDFLFQHLLNQVDMSTADSKAKLAQLAI
PLMSKLPDTVFRQMMEGRLIALLGIEKEGLNKLLPAAASNSRIDKRQKVTPMRLAISVLLQHPNIAYGLPEFPEFKEMTL
PGLSLLNSLLEICRTTPTITTGQLLEHWREKPESSQLNKLAVWQNDIKEDYYEAFFLDTLENFLNFHLTSKIEFLKQKSR
LGQALTSAETQQLALLLAEQKQH

Sequences:

>Translated_583_residues
MAGRIPRNFIDDLIARTDIVDFIDSRVKLKKQGKDYRACCPFHNGNNNSSFSVSSDKQFYHCFNCGVSGNVLSFIMEYDG
IEFVDAIETLAEQLSIEVPREESGFGKAHQSSYVDRKDLYQLMADIAVFYQQQLRTHKNSQQVINYLKGRGLSGQTVKHF
KIGYAPDGWNEVRKRFAVSADLEKQLIEAGMLISKEKSGSYDRFRDRLMFPIHDRRGRVIGFGGRVLGDNEPKYLNSPET
PLFHKGKELYGLYQVKQAYKEIKRVLVVEGYMDVVALGQFGIDYAVASLGTSTTPEHIQQLFRHTSEVICCFDGDKAGKA
AAWRALENALPYIQDGRTLRFMFLADGEDPDSYVQKEGKEAFEKLIEQALPLSDFLFQHLLNQVDMSTADSKAKLAQLAI
PLMSKLPDTVFRQMMEGRLIALLGIEKEGLNKLLPAAASNSRIDKRQKVTPMRLAISVLLQHPNIAYGLPEFPEFKEMTL
PGLSLLNSLLEICRTTPTITTGQLLEHWREKPESSQLNKLAVWQNDIKEDYYEAFFLDTLENFLNFHLTSKIEFLKQKSR
LGQALTSAETQQLALLLAEQKQH
>Mature_582_residues
AGRIPRNFIDDLIARTDIVDFIDSRVKLKKQGKDYRACCPFHNGNNNSSFSVSSDKQFYHCFNCGVSGNVLSFIMEYDGI
EFVDAIETLAEQLSIEVPREESGFGKAHQSSYVDRKDLYQLMADIAVFYQQQLRTHKNSQQVINYLKGRGLSGQTVKHFK
IGYAPDGWNEVRKRFAVSADLEKQLIEAGMLISKEKSGSYDRFRDRLMFPIHDRRGRVIGFGGRVLGDNEPKYLNSPETP
LFHKGKELYGLYQVKQAYKEIKRVLVVEGYMDVVALGQFGIDYAVASLGTSTTPEHIQQLFRHTSEVICCFDGDKAGKAA
AWRALENALPYIQDGRTLRFMFLADGEDPDSYVQKEGKEAFEKLIEQALPLSDFLFQHLLNQVDMSTADSKAKLAQLAIP
LMSKLPDTVFRQMMEGRLIALLGIEKEGLNKLLPAAASNSRIDKRQKVTPMRLAISVLLQHPNIAYGLPEFPEFKEMTLP
GLSLLNSLLEICRTTPTITTGQLLEHWREKPESSQLNKLAVWQNDIKEDYYEAFFLDTLENFLNFHLTSKIEFLKQKSRL
GQALTSAETQQLALLLAEQKQH

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=563, Percent_Identity=53.1083481349911, Blast_Score=601, Evalue=1e-173,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 66211; Mature: 66079

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRIPRNFIDDLIARTDIVDFIDSRVKLKKQGKDYRACCPFHNGNNNSSFSVSSDKQFY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEECCCCCEE
HCFNCGVSGNVLSFIMEYDGIEFVDAIETLAEQLSIEVPREESGFGKAHQSSYVDRKDLY
EEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHH
QLMADIAVFYQQQLRTHKNSQQVINYLKGRGLSGQTVKHFKIGYAPDGWNEVRKRFAVSA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
DLEKQLIEAGMLISKEKSGSYDRFRDRLMFPIHDRRGRVIGFGGRVLGDNEPKYLNSPET
HHHHHHHHHHHHEECCCCCCHHHHHHCCCCCEECCCCCEEEECCEEECCCCCCCCCCCCC
PLFHKGKELYGLYQVKQAYKEIKRVLVVEGYMDVVALGQFGIDYAVASLGTSTTPEHIQQ
CCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LFRHTSEVICCFDGDKAGKAAAWRALENALPYIQDGRTLRFMFLADGEDPDSYVQKEGKE
HHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHH
AFEKLIEQALPLSDFLFQHLLNQVDMSTADSKAKLAQLAIPLMSKLPDTVFRQMMEGRLI
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCEE
ALLGIEKEGLNKLLPAAASNSRIDKRQKVTPMRLAISVLLQHPNIAYGLPEFPEFKEMTL
EEEECCHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHCC
PGLSLLNSLLEICRTTPTITTGQLLEHWREKPESSQLNKLAVWQNDIKEDYYEAFFLDTL
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ENFLNFHLTSKIEFLKQKSRLGQALTSAETQQLALLLAEQKQH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGRIPRNFIDDLIARTDIVDFIDSRVKLKKQGKDYRACCPFHNGNNNSSFSVSSDKQFY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEECCCCCEE
HCFNCGVSGNVLSFIMEYDGIEFVDAIETLAEQLSIEVPREESGFGKAHQSSYVDRKDLY
EEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHH
QLMADIAVFYQQQLRTHKNSQQVINYLKGRGLSGQTVKHFKIGYAPDGWNEVRKRFAVSA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
DLEKQLIEAGMLISKEKSGSYDRFRDRLMFPIHDRRGRVIGFGGRVLGDNEPKYLNSPET
HHHHHHHHHHHHEECCCCCCHHHHHHCCCCCEECCCCCEEEECCEEECCCCCCCCCCCCC
PLFHKGKELYGLYQVKQAYKEIKRVLVVEGYMDVVALGQFGIDYAVASLGTSTTPEHIQQ
CCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LFRHTSEVICCFDGDKAGKAAAWRALENALPYIQDGRTLRFMFLADGEDPDSYVQKEGKE
HHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHH
AFEKLIEQALPLSDFLFQHLLNQVDMSTADSKAKLAQLAIPLMSKLPDTVFRQMMEGRLI
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCEE
ALLGIEKEGLNKLLPAAASNSRIDKRQKVTPMRLAISVLLQHPNIAYGLPEFPEFKEMTL
EEEECCHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHCC
PGLSLLNSLLEICRTTPTITTGQLLEHWREKPESSQLNKLAVWQNDIKEDYYEAFFLDTL
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ENFLNFHLTSKIEFLKQKSRLGQALTSAETQQLALLLAEQKQH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]