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The map label for this gene is nadB [H]

Identifier: 119943857

GI number: 119943857

Start: 86239

End: 87870

Strand: Reverse

Name: nadB [H]

Synonym: Ping_0064

Alternate gene names: 119943857

Gene position: 87870-86239 (Counterclockwise)

Preceding gene: 119943863

Following gene: 119943855

Centisome position: 1.93

GC content: 42.65

Gene sequence:

>1632_bases
ATGACAGAAAGTAAATCAATAAGTGAAAAGCAACATACTTGTGACGTCCTGATTATTGGCGCTGGCGCTGCCGGGCTTTC
ATTAGCGCTAAAGCTCGCCCCGCACGCCAGAATCAATGTATTAAGTAAAGGTAAGCTTTCTGAAGGCTCAACCTTATACG
CCCAAGGGGGCGTTGCTGCTGTTTTTTCCGATGACGATAGTATTGAAGCCCACGTCAAAGACACATTAATCGCAGGCGCT
GATTTATGTGATGAAGAAGCGGTTCGCTTTACCGTCAGTAATGCTAAAGAGAGCATTCAATGGTTAATTGATACCGGGGT
TGGTTTTGATACTGAAATTAATGACGATGGACAAGCACAATACCACCTCACGCGAGAAGGCGGGCATTCTCATCGCCGTA
TTCTTCACTCTGCTGATGCGACGGGAAAAGAGATAGAAACAACTTTACTCAGCGCCGTCAAAAAACATAAAAATATCAAC
CTGATGGAGGGTTATAATGCGGTTGATTTAATCACTAGCCGACAATTAAACCTACCGGCAAACCGCATTATAGGCGCTTA
TGTCTGGAACCGTAAAGCCGAGCGAGTTGAAATCATTAAGGCGAATTTTGTAACATTGGCAACAGGCGGTGCCAGTAAAG
TTTATCAGTATACCAGTAATCCCGATGTCTCCAGCGGTGATGGTATTGCTTTAGCCTGGCGCAGTGGCTGCCGTATTGCC
AATATGGAGTTTAATCAGTTTCATCCTACCTGCTTATACCATACCGATGCTAAAAACTTTTTGTTAAGTGAGGCGCTCCG
TGGAGAAGGTGCTTATCTGCGGCGGCCCGATGGCACTCGCTTTATGCTTGATTTTGATGAGCGTGCAGAGCTCGCTCCCC
GTGACATTGTTGCACGGGCAATAGATTATGAGATGAAACGTTTAGGTGCAGATTGTATGTATTTAGACATTAGCCATAAA
TCCAAAGAATTCATCGTTAAACACTTTCCAACTATTTATCATCGCTGCTTATCACTGGGTATTGATATGGCCGAGCAACC
AATACCTATTGTACCCGCAGCCCATTATAGTTGTGGCGGAGTAATGATCGATCACCATGGACAAACGGATATAGATGGAT
TATATGCGATTGGAGAAGTCTCTTATACCGGCTTGCATGGCGCAAACCGCTTGGCCTCTAATTCGTTATTAGAGTGTATT
GTTTATGCACACGCCTGCGCCAAAGATATTATTAACCGAATTCCTTTTTATAAAGAACGTAAATTTGCAAAAATTCCTGA
CTGGGATGAGAGTCAAGTAGATGATTCAGATGAAGCAGTGGTCATTCAGCACAACTGGCATGAACTGCGATTATTTATGT
GGGATTATGTCGGTATTGTTCGAACCGACAAACGTCTAGAACGTGCTCTGCATAGGGTGCAGTTATTGCAGCAAGAAATT
GATGAATATTACGGTAACTTTAAAGTAAGCAATAACCTTCTGGAACTTAGAAATTTAGTACAAGTTGCTGAAATAATAAT
AAAAAGTGCAATGAAACGAAAAGAAAGCCGAGGTTTACACTACAATCTTGATTATCCTGAACAACTGGACAATCCAAAAC
CCACTATTCTCACTCCTGAAAAAAGCTGTTAA

Upstream 100 bases:

>100_bases
CCTAAAGTAGTTGAAAAGTATAGATTCTCATTTTAAAGTGACTGATATCAAAATTAAATTTATACTAAAAAACGATCTTA
ATAACAGAGCATATAACGCT

Downstream 100 bases:

>100_bases
CCATCACTACTGCCCGATTTTAATTAATCGGGCTAATAACCGGTACTCATCTTCTTTAACGGCATCTTTGTAAATTACAA
TAAACTTCTTAGAGCGCTTT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MTESKSISEKQHTCDVLIIGAGAAGLSLALKLAPHARINVLSKGKLSEGSTLYAQGGVAAVFSDDDSIEAHVKDTLIAGA
DLCDEEAVRFTVSNAKESIQWLIDTGVGFDTEINDDGQAQYHLTREGGHSHRRILHSADATGKEIETTLLSAVKKHKNIN
LMEGYNAVDLITSRQLNLPANRIIGAYVWNRKAERVEIIKANFVTLATGGASKVYQYTSNPDVSSGDGIALAWRSGCRIA
NMEFNQFHPTCLYHTDAKNFLLSEALRGEGAYLRRPDGTRFMLDFDERAELAPRDIVARAIDYEMKRLGADCMYLDISHK
SKEFIVKHFPTIYHRCLSLGIDMAEQPIPIVPAAHYSCGGVMIDHHGQTDIDGLYAIGEVSYTGLHGANRLASNSLLECI
VYAHACAKDIINRIPFYKERKFAKIPDWDESQVDDSDEAVVIQHNWHELRLFMWDYVGIVRTDKRLERALHRVQLLQQEI
DEYYGNFKVSNNLLELRNLVQVAEIIIKSAMKRKESRGLHYNLDYPEQLDNPKPTILTPEKSC

Sequences:

>Translated_543_residues
MTESKSISEKQHTCDVLIIGAGAAGLSLALKLAPHARINVLSKGKLSEGSTLYAQGGVAAVFSDDDSIEAHVKDTLIAGA
DLCDEEAVRFTVSNAKESIQWLIDTGVGFDTEINDDGQAQYHLTREGGHSHRRILHSADATGKEIETTLLSAVKKHKNIN
LMEGYNAVDLITSRQLNLPANRIIGAYVWNRKAERVEIIKANFVTLATGGASKVYQYTSNPDVSSGDGIALAWRSGCRIA
NMEFNQFHPTCLYHTDAKNFLLSEALRGEGAYLRRPDGTRFMLDFDERAELAPRDIVARAIDYEMKRLGADCMYLDISHK
SKEFIVKHFPTIYHRCLSLGIDMAEQPIPIVPAAHYSCGGVMIDHHGQTDIDGLYAIGEVSYTGLHGANRLASNSLLECI
VYAHACAKDIINRIPFYKERKFAKIPDWDESQVDDSDEAVVIQHNWHELRLFMWDYVGIVRTDKRLERALHRVQLLQQEI
DEYYGNFKVSNNLLELRNLVQVAEIIIKSAMKRKESRGLHYNLDYPEQLDNPKPTILTPEKSC
>Mature_542_residues
TESKSISEKQHTCDVLIIGAGAAGLSLALKLAPHARINVLSKGKLSEGSTLYAQGGVAAVFSDDDSIEAHVKDTLIAGAD
LCDEEAVRFTVSNAKESIQWLIDTGVGFDTEINDDGQAQYHLTREGGHSHRRILHSADATGKEIETTLLSAVKKHKNINL
MEGYNAVDLITSRQLNLPANRIIGAYVWNRKAERVEIIKANFVTLATGGASKVYQYTSNPDVSSGDGIALAWRSGCRIAN
MEFNQFHPTCLYHTDAKNFLLSEALRGEGAYLRRPDGTRFMLDFDERAELAPRDIVARAIDYEMKRLGADCMYLDISHKS
KEFIVKHFPTIYHRCLSLGIDMAEQPIPIVPAAHYSCGGVMIDHHGQTDIDGLYAIGEVSYTGLHGANRLASNSLLECIV
YAHACAKDIINRIPFYKERKFAKIPDWDESQVDDSDEAVVIQHNWHELRLFMWDYVGIVRTDKRLERALHRVQLLQQEID
EYYGNFKVSNNLLELRNLVQVAEIIIKSAMKRKESRGLHYNLDYPEQLDNPKPTILTPEKSC

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=531, Percent_Identity=30.3201506591337, Blast_Score=182, Evalue=6e-46,
Organism=Escherichia coli, GI1788928, Length=530, Percent_Identity=69.811320754717, Blast_Score=800, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=524, Percent_Identity=30.5343511450382, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1786942, Length=535, Percent_Identity=28.785046728972, Blast_Score=183, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17505833, Length=534, Percent_Identity=29.9625468164794, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17550100, Length=534, Percent_Identity=29.4007490636704, Blast_Score=189, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI71986328, Length=433, Percent_Identity=27.4826789838337, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322701, Length=528, Percent_Identity=29.7348484848485, Blast_Score=193, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6322416, Length=534, Percent_Identity=28.8389513108614, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6322511, Length=484, Percent_Identity=25.4132231404959, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137288, Length=549, Percent_Identity=29.143897996357, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24655642, Length=549, Percent_Identity=29.143897996357, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24655647, Length=549, Percent_Identity=29.143897996357, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24663005, Length=532, Percent_Identity=29.1353383458647, Blast_Score=183, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 60803; Mature: 60671

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESKSISEKQHTCDVLIIGAGAAGLSLALKLAPHARINVLSKGKLSEGSTLYAQGGVAA
CCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCEEEEEECCCCCCCCEEEECCCEEE
VFSDDDSIEAHVKDTLIAGADLCDEEAVRFTVSNAKESIQWLIDTGVGFDTEINDDGQAQ
EECCCCCCHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHEECCCCCCCCCCCCCCEE
YHLTREGGHSHRRILHSADATGKEIETTLLSAVKKHKNINLMEGYNAVDLITSRQLNLPA
EEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCCCCCH
NRIIGAYVWNRKAERVEIIKANFVTLATGGASKVYQYTSNPDVSSGDGIALAWRSGCRIA
HHEEEHHHHCCCCCEEEEEEEEEEEEECCCCCCEEEECCCCCCCCCCCEEEEECCCCEEE
NMEFNQFHPTCLYHTDAKNFLLSEALRGEGAYLRRPDGTRFMLDFDERAELAPRDIVARA
ECCCCCCCCEEEEECCHHHHHHHHHHCCCCCEEECCCCCEEEEECCCCCCCCHHHHHHHH
IDYEMKRLGADCMYLDISHKSKEFIVKHFPTIYHRCLSLGIDMAEQPIPIVPAAHYSCGG
HHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
VMIDHHGQTDIDGLYAIGEVSYTGLHGANRLASNSLLECIVYAHACAKDIINRIPFYKER
EEEECCCCCCCCCEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KFAKIPDWDESQVDDSDEAVVIQHNWHELRLFMWDYVGIVRTDKRLERALHRVQLLQQEI
CCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEHHHHHHEECHHHHHHHHHHHHHHHHHH
DEYYGNFKVSNNLLELRNLVQVAEIIIKSAMKRKESRGLHYNLDYPEQLDNPKPTILTPE
HHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHCCCCCCCEECCC
KSC
CCC
>Mature Secondary Structure 
TESKSISEKQHTCDVLIIGAGAAGLSLALKLAPHARINVLSKGKLSEGSTLYAQGGVAA
CCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCEEEEEECCCCCCCCEEEECCCEEE
VFSDDDSIEAHVKDTLIAGADLCDEEAVRFTVSNAKESIQWLIDTGVGFDTEINDDGQAQ
EECCCCCCHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHEECCCCCCCCCCCCCCEE
YHLTREGGHSHRRILHSADATGKEIETTLLSAVKKHKNINLMEGYNAVDLITSRQLNLPA
EEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCCCCCH
NRIIGAYVWNRKAERVEIIKANFVTLATGGASKVYQYTSNPDVSSGDGIALAWRSGCRIA
HHEEEHHHHCCCCCEEEEEEEEEEEEECCCCCCEEEECCCCCCCCCCCEEEEECCCCEEE
NMEFNQFHPTCLYHTDAKNFLLSEALRGEGAYLRRPDGTRFMLDFDERAELAPRDIVARA
ECCCCCCCCEEEEECCHHHHHHHHHHCCCCCEEECCCCCEEEEECCCCCCCCHHHHHHHH
IDYEMKRLGADCMYLDISHKSKEFIVKHFPTIYHRCLSLGIDMAEQPIPIVPAAHYSCGG
HHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
VMIDHHGQTDIDGLYAIGEVSYTGLHGANRLASNSLLECIVYAHACAKDIINRIPFYKER
EEEECCCCCCCCCEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KFAKIPDWDESQVDDSDEAVVIQHNWHELRLFMWDYVGIVRTDKRLERALHRVQLLQQEI
CCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEHHHHHHEECHHHHHHHHHHHHHHHHHH
DEYYGNFKVSNNLLELRNLVQVAEIIIKSAMKRKESRGLHYNLDYPEQLDNPKPTILTPE
HHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHCCCCCCCEECCC
KSC
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]