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The map label for this gene is ygfZ [H]

Identifier: 119943855

GI number: 119943855

Start: 84139

End: 85116

Strand: Reverse

Name: ygfZ [H]

Synonym: Ping_0062

Alternate gene names: 119943855

Gene position: 85116-84139 (Counterclockwise)

Preceding gene: 119943857

Following gene: 229358030

Centisome position: 1.87

GC content: 39.37

Gene sequence:

>978_bases
ATGTTTAAACTTAGTCAACTCAATAATATTAGCCAGCTGCCCGACTTAGTCCTATGCCCGCTAACTAGCTGGGACTTACT
TAGCGTAACAGGTGAAGATCGTATCACCTTCTTACAAGGACAGCTCACATGTGATTTGACGATTTTAAAACCAGGAGAGC
AAACACTGGCCGCGCAGTGTAACCCGCAGGGAAAAGTTTGGAGTATTTTTCGAGTTGTTGTCTTAAATGATAGAATTTTA
CTCATTCAGCCGAAATCAGTAACGGCAAAACAGCTACCTGAATTACAAAAATACGCGACCTTTTCGAAAGTTGAAATAAA
AAAAGAAACTGAATACCAATTATTGGGTTTAGCCGGCTGCAAATCTGCTGCAAATATTGCTAAGAATTTCAATATATCAG
CCACTCATGAAGAGAGTCACCTGCTAGATGATGATCAGGGCGTTATCATCAAACAACCCTATCCATCATTACGTTATTTG
ATGATTGTAAAAAACCAGCAAGCAACACAGTTAACTGAAGACCTAAAAGATCAGGCCAGTGTTTATGATGATAGTTTATG
GAATGCGATGAATATTGCTGCAGGCATCGCCTTTATCGAGGAAGAAACATCTGGATTGTTTATTCCTCAAATGCTCAATC
TACAGGCTCTTAATGGTATCAGTTTCACTAAAGGTTGTTATATTGGTCAAGAAACCATTGCTCGGACAAAATACAGAGGA
GCAAACAAACGGGCACTCTTTATTCTCACTGGCCGAGCAACTGAAGCGCCCAAAGCAGGACAAAATGTAAAAGTTTTATT
AAACAATAATTGGAAGCGTGTTGGTACTATCATATCCGGATGCCAATACGGCGATGGACATATCGAAGTATTAGCCATTT
TGCCAAAAAACAGTGAGTCAGAGAGTGTTTATCAAATACAAGGTATAGAAGGTTCAACCCTTTATTATGCCCCTCTACCC
TATCAAATAGACGATTAA

Upstream 100 bases:

>100_bases
CATCATAACAATAATAAATTATTAACAGCAACTACTGACAAATTATGTTGTGAGCTATACCATAGTAAAATATCATAACC
GTATAGAGATGAGGCATAAA

Downstream 100 bases:

>100_bases
TAACTCAGCTAAAACGCACAACAGGTAAGAAATATGTTTTTTTATTACTGTTGTGCGTTTACTTCTTAAGCCTTACCCAC
AAGCTAATTGACTTTAAATT

Product: glycine cleavage T protein (aminomethyl transferase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MFKLSQLNNISQLPDLVLCPLTSWDLLSVTGEDRITFLQGQLTCDLTILKPGEQTLAAQCNPQGKVWSIFRVVVLNDRIL
LIQPKSVTAKQLPELQKYATFSKVEIKKETEYQLLGLAGCKSAANIAKNFNISATHEESHLLDDDQGVIIKQPYPSLRYL
MIVKNQQATQLTEDLKDQASVYDDSLWNAMNIAAGIAFIEEETSGLFIPQMLNLQALNGISFTKGCYIGQETIARTKYRG
ANKRALFILTGRATEAPKAGQNVKVLLNNNWKRVGTIISGCQYGDGHIEVLAILPKNSESESVYQIQGIEGSTLYYAPLP
YQIDD

Sequences:

>Translated_325_residues
MFKLSQLNNISQLPDLVLCPLTSWDLLSVTGEDRITFLQGQLTCDLTILKPGEQTLAAQCNPQGKVWSIFRVVVLNDRIL
LIQPKSVTAKQLPELQKYATFSKVEIKKETEYQLLGLAGCKSAANIAKNFNISATHEESHLLDDDQGVIIKQPYPSLRYL
MIVKNQQATQLTEDLKDQASVYDDSLWNAMNIAAGIAFIEEETSGLFIPQMLNLQALNGISFTKGCYIGQETIARTKYRG
ANKRALFILTGRATEAPKAGQNVKVLLNNNWKRVGTIISGCQYGDGHIEVLAILPKNSESESVYQIQGIEGSTLYYAPLP
YQIDD
>Mature_325_residues
MFKLSQLNNISQLPDLVLCPLTSWDLLSVTGEDRITFLQGQLTCDLTILKPGEQTLAAQCNPQGKVWSIFRVVVLNDRIL
LIQPKSVTAKQLPELQKYATFSKVEIKKETEYQLLGLAGCKSAANIAKNFNISATHEESHLLDDDQGVIIKQPYPSLRYL
MIVKNQQATQLTEDLKDQASVYDDSLWNAMNIAAGIAFIEEETSGLFIPQMLNLQALNGISFTKGCYIGQETIARTKYRG
ANKRALFILTGRATEAPKAGQNVKVLLNNNWKRVGTIISGCQYGDGHIEVLAILPKNSESESVYQIQGIEGSTLYYAPLP
YQIDD

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=324, Percent_Identity=37.6543209876543, Blast_Score=239, Evalue=2e-64,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 36104; Mature: 36104

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKLSQLNNISQLPDLVLCPLTSWDLLSVTGEDRITFLQGQLTCDLTILKPGEQTLAAQC
CCCCHHCCCHHHCCCEEEECCCCCEEEEECCCCEEEEEECEEEEEEEEECCCCCEEEEEC
NPQGKVWSIFRVVVLNDRILLIQPKSVTAKQLPELQKYATFSKVEIKKETEYQLLGLAGC
CCCCCEEEEEEEEEECCEEEEEECCCCCHHHCHHHHHHCCCEEEEEECCCCEEEEEECCC
KSAANIAKNFNISATHEESHLLDDDQGVIIKQPYPSLRYLMIVKNQQATQLTEDLKDQAS
CHHHHHHHCCCEEEECCHHCCCCCCCCEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHH
VYDDSLWNAMNIAAGIAFIEEETSGLFIPQMLNLQALNGISFTKGCYIGQETIARTKYRG
HHHHHHHHHHHHHHHEEEEEECCCCEECHHHHCEEECCCCCCCCCEEECHHHHHHHHCCC
ANKRALFILTGRATEAPKAGQNVKVLLNNNWKRVGTIISGCQYGDGHIEVLAILPKNSES
CCCEEEEEEECCCCCCCCCCCCEEEEECCCCCEEHHHHHCCCCCCCCEEEEEEECCCCCC
ESVYQIQGIEGSTLYYAPLPYQIDD
CCEEEEECCCCCEEEEECCCCEECC
>Mature Secondary Structure
MFKLSQLNNISQLPDLVLCPLTSWDLLSVTGEDRITFLQGQLTCDLTILKPGEQTLAAQC
CCCCHHCCCHHHCCCEEEECCCCCEEEEECCCCEEEEEECEEEEEEEEECCCCCEEEEEC
NPQGKVWSIFRVVVLNDRILLIQPKSVTAKQLPELQKYATFSKVEIKKETEYQLLGLAGC
CCCCCEEEEEEEEEECCEEEEEECCCCCHHHCHHHHHHCCCEEEEEECCCCEEEEEECCC
KSAANIAKNFNISATHEESHLLDDDQGVIIKQPYPSLRYLMIVKNQQATQLTEDLKDQAS
CHHHHHHHCCCEEEECCHHCCCCCCCCEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHH
VYDDSLWNAMNIAAGIAFIEEETSGLFIPQMLNLQALNGISFTKGCYIGQETIARTKYRG
HHHHHHHHHHHHHHHEEEEEECCCCEECHHHHCEEECCCCCCCCCEEECHHHHHHHHCCC
ANKRALFILTGRATEAPKAGQNVKVLLNNNWKRVGTIISGCQYGDGHIEVLAILPKNSES
CCCEEEEEEECCCCCCCCCCCCEEEEECCCCCEEHHHHHCCCCCCCCEEEEEEECCCCCC
ESVYQIQGIEGSTLYYAPLPYQIDD
CCEEEEECCCCCEEEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA