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The map label for this gene is ksdD [H]

Identifier: 161485585

GI number: 161485585

Start: 4978280

End: 4979971

Strand: Direct

Name: ksdD [H]

Synonym: Mkms_4750

Alternate gene names: 161485585

Gene position: 4978280-4979971 (Clockwise)

Preceding gene: 119870773

Following gene: 119870780

Centisome position: 86.77

GC content: 67.08

Gene sequence:

>1692_bases
ATGACCGGACAGGAGTACGACGTCGTCGTGGTGGGCAGTGGTGCCGCCGGCATGGTCGCCGCCCTCACCGCAGCTCATCA
GGGTCTCTCGACTATAGTCGTTGAGAAGGCTCCGCACTATGGGGGTTCCACTGCGCGGTCAGGTGGTGGCGTATGGATCC
CGAACAACGAGATCCTCAAGCGCGACGGCGTGAAAGACACGCCCGAGGCGGCGCGCCAGTACCTGCGCGCGATCATCGGC
GACGTCGTCGAACCGGAACGCATCGACACCTACCTCCAACGCGGACCCGAGATGCTGACGTTCGTCCTCGAGCACTCACC
CCTGAAGCTGTGCTGGGTGCCGGGCTACTCCGACTACTACCCGGAGACACCCGGCGGAAAGCCGACCGGGCGGTCGGTGG
AGCCGAAGCCGTTCGACGCCAAGAAGCTCGGGGAGGATCTGCCCGGCCTGGAACCGCCGTACGGCAAGGTCCCGATGAAC
ATGGTCGTCATGCAACAGGACTATGTCCGGCTCAATCAACTCAAGCGCCACCCGCGTGGCGTACTGCGCAGCCTCAAGGT
CGGCATCCGCGCGACATGGGCCAAGGCCACCGGCAAGAACCTGGTCGGGATGGGGCGCGCGTTGATCGCCCCGCTGCGGA
TCGCCTTGCGCGAGGCCGGGGTACCGGTGCTGCTCAACACCGCGCTGACCGACCTCTACGTCGAGGACGGGGTGGTCCGC
GGGATCTACGTAGCGGATACGAACGCGCAGACAGGCACCGACAGCAGTGAGCCGCGGCTCATCCGCGCGCGCCGAGGGGT
GATCCTGGGCTCCGGCGGTTTCGAGCACAACGAGCAGATGCGCGTGAAGTATCAGCGCGCCCCGATCACCACCGAGTGGA
CCGTCGGCGCCAAGGCCAACACCGGTGATGGCATCCTGGCCGCCGAAAAGCTGGGCGCCGCGCTGGAACTGATGGAGGAC
GCCTGGTGGGGCCCGACGGTGCCGTTGGTCGGCGCCCCGTGGTTCGCCCTGTCCGAGCGCAACTCCCCCGGCTCGATCAT
CGTCAACATGTCCGGTAAGCGGTTCATGAACGAGTCGATGCCGTATGTCGAGGCGTGCCACCACATGTACGGCGGCGAAT
ACGGGCAGGGACCCGGCCCGGGCGAGAACATCCCGGCCTGGCTGATCTTCGACCAGCAGTACCGCGACCGCTACATCTTC
GCCGGACTGCAACCGGGACAACGGATTCCGAGCAAGTGGCTGGAGTCGGGCGTGATCGTCAAGGCCGACACCCTGGCCGA
GTTGGCCGAGAAGGCCGGGCTGCCCGCCGATGCGCTCGCCGCGACGGTCCAGCGGTTCAACGGATTCGCGCGGTCGGGCA
CCGACGAGGACTTCCACCGCGGTGAGAGCGCGTACGACCGCTACTACGGAGACCCGACGAACAAACCGAACCCCAACCTC
GGCGAGATCAGCCACGGTCCGTTCTACGCGGCGAAGATGGTGCCCGGTGACCTGGGCACCAAGGGCGGGGTCCGCACCGA
TGTGCACGGTCGGGCGCTGCGCGACGACGGTTCGGTGATCGAGGGCCTGTACGCGGCCGGCAACGTCAGCTCACCGGTCA
TGGGCCACACGTATCCGGGCCCGGGCGGGACGATCGGCCCGGCCATGACGTTCGGGTATCTGGCGGCGCTGCACCTGGCT
GGTAGAAGTTAG

Upstream 100 bases:

>100_bases
CTGCTCACGTACCTCGGAACTGAGCATCTAGGTGAAGTCCCCTCGTTTCGTCGACCGGCGCAGTTGTGGGTCGGCCGGGC
AATTCTATAACGTGTTCTAC

Downstream 100 bases:

>100_bases
CCATGCCGATCAATCTGGACGAGGCGCTGGGCGCCGAGCTGCCACCCGCCGAATTCTCCTGGACGAGCAGCGATGTGCAG
CTCTACCACCTGGGGCTGGG

Product: 3-ketosteroid-delta-1-dehydrogenase

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 563; Mature: 562

Protein sequence:

>563_residues
MTGQEYDVVVVGSGAAGMVAALTAAHQGLSTIVVEKAPHYGGSTARSGGGVWIPNNEILKRDGVKDTPEAARQYLRAIIG
DVVEPERIDTYLQRGPEMLTFVLEHSPLKLCWVPGYSDYYPETPGGKPTGRSVEPKPFDAKKLGEDLPGLEPPYGKVPMN
MVVMQQDYVRLNQLKRHPRGVLRSLKVGIRATWAKATGKNLVGMGRALIAPLRIALREAGVPVLLNTALTDLYVEDGVVR
GIYVADTNAQTGTDSSEPRLIRARRGVILGSGGFEHNEQMRVKYQRAPITTEWTVGAKANTGDGILAAEKLGAALELMED
AWWGPTVPLVGAPWFALSERNSPGSIIVNMSGKRFMNESMPYVEACHHMYGGEYGQGPGPGENIPAWLIFDQQYRDRYIF
AGLQPGQRIPSKWLESGVIVKADTLAELAEKAGLPADALAATVQRFNGFARSGTDEDFHRGESAYDRYYGDPTNKPNPNL
GEISHGPFYAAKMVPGDLGTKGGVRTDVHGRALRDDGSVIEGLYAAGNVSSPVMGHTYPGPGGTIGPAMTFGYLAALHLA
GRS

Sequences:

>Translated_563_residues
MTGQEYDVVVVGSGAAGMVAALTAAHQGLSTIVVEKAPHYGGSTARSGGGVWIPNNEILKRDGVKDTPEAARQYLRAIIG
DVVEPERIDTYLQRGPEMLTFVLEHSPLKLCWVPGYSDYYPETPGGKPTGRSVEPKPFDAKKLGEDLPGLEPPYGKVPMN
MVVMQQDYVRLNQLKRHPRGVLRSLKVGIRATWAKATGKNLVGMGRALIAPLRIALREAGVPVLLNTALTDLYVEDGVVR
GIYVADTNAQTGTDSSEPRLIRARRGVILGSGGFEHNEQMRVKYQRAPITTEWTVGAKANTGDGILAAEKLGAALELMED
AWWGPTVPLVGAPWFALSERNSPGSIIVNMSGKRFMNESMPYVEACHHMYGGEYGQGPGPGENIPAWLIFDQQYRDRYIF
AGLQPGQRIPSKWLESGVIVKADTLAELAEKAGLPADALAATVQRFNGFARSGTDEDFHRGESAYDRYYGDPTNKPNPNL
GEISHGPFYAAKMVPGDLGTKGGVRTDVHGRALRDDGSVIEGLYAAGNVSSPVMGHTYPGPGGTIGPAMTFGYLAALHLA
GRS
>Mature_562_residues
TGQEYDVVVVGSGAAGMVAALTAAHQGLSTIVVEKAPHYGGSTARSGGGVWIPNNEILKRDGVKDTPEAARQYLRAIIGD
VVEPERIDTYLQRGPEMLTFVLEHSPLKLCWVPGYSDYYPETPGGKPTGRSVEPKPFDAKKLGEDLPGLEPPYGKVPMNM
VVMQQDYVRLNQLKRHPRGVLRSLKVGIRATWAKATGKNLVGMGRALIAPLRIALREAGVPVLLNTALTDLYVEDGVVRG
IYVADTNAQTGTDSSEPRLIRARRGVILGSGGFEHNEQMRVKYQRAPITTEWTVGAKANTGDGILAAEKLGAALELMEDA
WWGPTVPLVGAPWFALSERNSPGSIIVNMSGKRFMNESMPYVEACHHMYGGEYGQGPGPGENIPAWLIFDQQYRDRYIFA
GLQPGQRIPSKWLESGVIVKADTLAELAEKAGLPADALAATVQRFNGFARSGTDEDFHRGESAYDRYYGDPTNKPNPNLG
EISHGPFYAAKMVPGDLGTKGGVRTDVHGRALRDDGSVIEGLYAAGNVSSPVMGHTYPGPGGTIGPAMTFGYLAALHLAG
RS

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 1,4- androstadiene-3,17-dione (ADD) from 4-androstene-3,17-dione (AD) to [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 60608; Mature: 60476

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGQEYDVVVVGSGAAGMVAALTAAHQGLSTIVVEKAPHYGGSTARSGGGVWIPNNEILK
CCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEECCCCHHHH
RDGVKDTPEAARQYLRAIIGDVVEPERIDTYLQRGPEMLTFVLEHSPLKLCWVPGYSDYY
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHEEHEECCCCEEEEEECCCCCCC
PETPGGKPTGRSVEPKPFDAKKLGEDLPGLEPPYGKVPMNMVVMQQDYVRLNQLKRHPRG
CCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHH
VLRSLKVGIRATWAKATGKNLVGMGRALIAPLRIALREAGVPVLLNTALTDLYVEDGVVR
HHHHHHHHHEEEEHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCEE
GIYVADTNAQTGTDSSEPRLIRARRGVILGSGGFEHNEQMRVKYQRAPITTEWTVGAKAN
EEEEEECCCCCCCCCCCCCEEEECCCEEEECCCCCCCCCEEEEEECCCCCEEEECCCCCC
TGDGILAAEKLGAALELMEDAWWGPTVPLVGAPWFALSERNSPGSIIVNMSGKRFMNESM
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCHHHHCCC
PYVEACHHMYGGEYGQGPGPGENIPAWLIFDQQYRDRYIFAGLQPGQRIPSKWLESGVIV
CHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCHHHHHCCCEE
KADTLAELAEKAGLPADALAATVQRFNGFARSGTDEDFHRGESAYDRYYGDPTNKPNPNL
EHHHHHHHHHHCCCCHHHHHHHHHHHCCHHCCCCCHHHHCCCHHHHHHCCCCCCCCCCCC
GEISHGPFYAAKMVPGDLGTKGGVRTDVHGRALRDDGSVIEGLYAAGNVSSPVMGHTYPG
CCCCCCCEEEEEECCCCCCCCCCCEECCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCCC
PGGTIGPAMTFGYLAALHLAGRS
CCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TGQEYDVVVVGSGAAGMVAALTAAHQGLSTIVVEKAPHYGGSTARSGGGVWIPNNEILK
CCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEECCCCHHHH
RDGVKDTPEAARQYLRAIIGDVVEPERIDTYLQRGPEMLTFVLEHSPLKLCWVPGYSDYY
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHEEHEECCCCEEEEEECCCCCCC
PETPGGKPTGRSVEPKPFDAKKLGEDLPGLEPPYGKVPMNMVVMQQDYVRLNQLKRHPRG
CCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHH
VLRSLKVGIRATWAKATGKNLVGMGRALIAPLRIALREAGVPVLLNTALTDLYVEDGVVR
HHHHHHHHHEEEEHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCEE
GIYVADTNAQTGTDSSEPRLIRARRGVILGSGGFEHNEQMRVKYQRAPITTEWTVGAKAN
EEEEEECCCCCCCCCCCCCEEEECCCEEEECCCCCCCCCEEEEEECCCCCEEEECCCCCC
TGDGILAAEKLGAALELMEDAWWGPTVPLVGAPWFALSERNSPGSIIVNMSGKRFMNESM
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCHHHHCCC
PYVEACHHMYGGEYGQGPGPGENIPAWLIFDQQYRDRYIFAGLQPGQRIPSKWLESGVIV
CHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCHHHHHCCCEE
KADTLAELAEKAGLPADALAATVQRFNGFARSGTDEDFHRGESAYDRYYGDPTNKPNPNL
EHHHHHHHHHHCCCCHHHHHHHHHHHCCHHCCCCCHHHHCCCHHHHHHCCCCCCCCCCCC
GEISHGPFYAAKMVPGDLGTKGGVRTDVHGRALRDDGSVIEGLYAAGNVSSPVMGHTYPG
CCCCCCCEEEEEECCCCCCCCCCCEECCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCCC
PGGTIGPAMTFGYLAALHLAGRS
CCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA