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The map label for this gene is ynfL [H]

Identifier: 119871391

GI number: 119871391

Start: 5604521

End: 5605447

Strand: Direct

Name: ynfL [H]

Synonym: Mkms_5367

Alternate gene names: 119871391

Gene position: 5604521-5605447 (Clockwise)

Preceding gene: 119871390

Following gene: 119871400

Centisome position: 97.69

GC content: 70.12

Gene sequence:

>927_bases
TTGGAACTCGACTTCACGCGACTGAAGTACTTCGTCGCCGTCGCCGACGAACTCCACTTCAAACGCGCGGCCGACCGGCT
CCGGATCACCCCGCCACCGCTGAGCAAGCAGATCAAACTGCTCGAACGCGAGCTCGGCGGCCCGTTGTTCGAGCGCAACT
ACCACGAGGTCCGGCTGACCCCGCTGGGCGAGCAGCTGGTCGGCCCGGCCCGCGACATCCTGCGGCGGGTCGACGAGTTC
AAGGCGACGGCGGCCCAGGCGGTGCAGGGTGCGGCGGCGCTGCGAATCGGGGCGACCGCGTATGCGCCCTCCGATCTGCT
CGCCCAGGTGGAGGCGGCCGCGGCGGACCTGCCCAATCCGTCGGTGTTCAGTGTCCCCGGGTCGGCCGCCGAGGTGATCG
CCAAACTCGTGTCCGGGCATCTCGAGATCGGCGTCATCCACCTGCCCGCGAAGGACAACCGTCTCGCGCACCGGGTCATC
GCCACCTACCCGGGCGCGATCGCGGTCCGCTTCGACGATCCGCTGGCCGCCTTCGACACGGTGACGGTCGAGCAGTTGCG
GGACCGCGAGGTGGTGATCGACTTCGCGCGGCCGAACCCCGTGGTGCTGGCCGCGCTGACCCGGCAACTCAGCGCGCGCG
GCGTGCACCGGATCGTGCGCACCACCAATCAGCGGGGCGGCGAACTCGAGATGGCGGCCCAGGTGTCGCACCGGCATCTG
GTGGCGATCCTCACCTACGCCCCCGCCTCGGCGATCGGCCGGATCTTCTCCCCACCGGAATTCAGACTGATCCCGATCGA
CGAGAGCACATGGCCGCCGGCCGAGATCGCGCTGGCGTGGTCGCGTGACCGCGCTCGAGCCATGCCGGAGATCGAGCAGG
CGGTGGAGCGGTTGGCGGATGCGGTGGAACGGGCGCGGTCGCACTAG

Upstream 100 bases:

>100_bases
CGGCGTGCTGCAGACGCATCGGCTCTGAGGTCGGCGGGTCCGCGGGACGGCTGCCGCGGGTCCGTACGATTCAGACAAAC
CGATCGTCGGACGAGGTGTC

Downstream 100 bases:

>100_bases
GGCTATACGCTCGCGAGTTCTGCACCACGGTCGTCGCCTGTGCGGTCCCACGACCCTGGTGTAGAAGTCGGCGCAAGACC
GGTTGTGCCAGTCGGCTGCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MELDFTRLKYFVAVADELHFKRAADRLRITPPPLSKQIKLLERELGGPLFERNYHEVRLTPLGEQLVGPARDILRRVDEF
KATAAQAVQGAAALRIGATAYAPSDLLAQVEAAAADLPNPSVFSVPGSAAEVIAKLVSGHLEIGVIHLPAKDNRLAHRVI
ATYPGAIAVRFDDPLAAFDTVTVEQLRDREVVIDFARPNPVVLAALTRQLSARGVHRIVRTTNQRGGELEMAAQVSHRHL
VAILTYAPASAIGRIFSPPEFRLIPIDESTWPPAEIALAWSRDRARAMPEIEQAVERLADAVERARSH

Sequences:

>Translated_308_residues
MELDFTRLKYFVAVADELHFKRAADRLRITPPPLSKQIKLLERELGGPLFERNYHEVRLTPLGEQLVGPARDILRRVDEF
KATAAQAVQGAAALRIGATAYAPSDLLAQVEAAAADLPNPSVFSVPGSAAEVIAKLVSGHLEIGVIHLPAKDNRLAHRVI
ATYPGAIAVRFDDPLAAFDTVTVEQLRDREVVIDFARPNPVVLAALTRQLSARGVHRIVRTTNQRGGELEMAAQVSHRHL
VAILTYAPASAIGRIFSPPEFRLIPIDESTWPPAEIALAWSRDRARAMPEIEQAVERLADAVERARSH
>Mature_308_residues
MELDFTRLKYFVAVADELHFKRAADRLRITPPPLSKQIKLLERELGGPLFERNYHEVRLTPLGEQLVGPARDILRRVDEF
KATAAQAVQGAAALRIGATAYAPSDLLAQVEAAAADLPNPSVFSVPGSAAEVIAKLVSGHLEIGVIHLPAKDNRLAHRVI
ATYPGAIAVRFDDPLAAFDTVTVEQLRDREVVIDFARPNPVVLAALTRQLSARGVHRIVRTTNQRGGELEMAAQVSHRHL
VAILTYAPASAIGRIFSPPEFRLIPIDESTWPPAEIALAWSRDRARAMPEIEQAVERLADAVERARSH

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=302, Percent_Identity=31.7880794701987, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788887, Length=214, Percent_Identity=30.3738317757009, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790399, Length=193, Percent_Identity=29.0155440414508, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI2367136, Length=273, Percent_Identity=30.7692307692308, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788297, Length=148, Percent_Identity=31.0810810810811, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790208, Length=177, Percent_Identity=30.5084745762712, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI157672245, Length=188, Percent_Identity=30.3191489361702, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33826; Mature: 33826

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELDFTRLKYFVAVADELHFKRAADRLRITPPPLSKQIKLLERELGGPLFERNYHEVRLT
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHCCCCCEEEEE
PLGEQLVGPARDILRRVDEFKATAAQAVQGAAALRIGATAYAPSDLLAQVEAAAADLPNP
ECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHCCCCC
SVFSVPGSAAEVIAKLVSGHLEIGVIHLPAKDNRLAHRVIATYPGAIAVRFDDPLAAFDT
CEEECCCHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCHHHHHH
VTVEQLRDREVVIDFARPNPVVLAALTRQLSARGVHRIVRTTNQRGGELEMAAQVSHRHL
HHHHHHCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCEE
VAILTYAPASAIGRIFSPPEFRLIPIDESTWPPAEIALAWSRDRARAMPEIEQAVERLAD
EEEEEECCHHHHHHCCCCCCEEEEECCCCCCCCHHEEEEECCHHHHCCHHHHHHHHHHHH
AVERARSH
HHHHHHCC
>Mature Secondary Structure
MELDFTRLKYFVAVADELHFKRAADRLRITPPPLSKQIKLLERELGGPLFERNYHEVRLT
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHCCCCCEEEEE
PLGEQLVGPARDILRRVDEFKATAAQAVQGAAALRIGATAYAPSDLLAQVEAAAADLPNP
ECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHCCCCC
SVFSVPGSAAEVIAKLVSGHLEIGVIHLPAKDNRLAHRVIATYPGAIAVRFDDPLAAFDT
CEEECCCHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCHHHHHH
VTVEQLRDREVVIDFARPNPVVLAALTRQLSARGVHRIVRTTNQRGGELEMAAQVSHRHL
HHHHHHCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCEE
VAILTYAPASAIGRIFSPPEFRLIPIDESTWPPAEIALAWSRDRARAMPEIEQAVERLAD
EEEEEECCHHHHHHCCCCCCEEEEECCCCCCCCHHEEEEECCHHHHCCHHHHHHHHHHHH
AVERARSH
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]