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The map label for this gene is tcmP [H]

Identifier: 119871346

GI number: 119871346

Start: 5556109

End: 5556909

Strand: Direct

Name: tcmP [H]

Synonym: Mkms_5320

Alternate gene names: 119871346

Gene position: 5556109-5556909 (Clockwise)

Preceding gene: 119871343

Following gene: 119871348

Centisome position: 96.84

GC content: 66.29

Gene sequence:

>801_bases
ATGCTGAGCGGTGTCTCGGAGACCGCGCTCCTGACGCTGAACGGCCGGGCGCACCAGGCGCGACACCCGAAGGCCATCAT
CGACGACCCGATGGCGATCCGCCTCGTCGACTCCATCGATTTCGACTTCGACAAGTTCGGCCGCAAGGGCCAGGAGATGG
CGTTGCGCTCGCTGGCCTTCGACCGGGCGGCGATCCAGTACCTCGACGCGCATCCGTCCGCGACGGTGGTGGCGTTGGCC
GAGGGTCTGCAGACCAGCTTCTGGCGGCTCACCAGCGCACGGCCGGACCTCGACTTCCACTGGGTAACAGTCGATTTCGA
ACCGATCATCGCACTGCGCGAACGGTTGCTGCCGCACTCCGACCGGATCAGCACTCTCGCGCAGTCGGCGCTGGACTTCT
CGTGGATGGACCACGTCGACACCAGCAACGGTGTGTTCATCACCGCCGAAGGCCTGCTGATGTACCTGCAGCCCCACGAG
TCGATGCGCCTGATCACCGAGTGCGCCAGGCGGTTTCCGGGCGGACAGATGATCTTCGACCTGCCGCCGGTGCTGGTGAA
GAAGCTGGCGCCCAACGGGATGCGGGCCTCCCGCCGGTACCGAGTCCCACCGATGCCGTTCAGCCTGACGCCGAATCAGC
TGGCCGATCTCGCGATCACCGTCCCCGGTGTCCGTGCGGTGCACGACCTTCCGATGCCGCGGGGCCGCGGCCTGCTGTTC
GAGAAGCTGTACCCGGCGTTCTGGGAGTTCAAGCCGACCAAGCAGATGCGCGGCGCCTACACGTTGCTCGAGTTCGGGTA
G

Upstream 100 bases:

>100_bases
ACTCATACCTGAACCGTATACACATCCCGCTGTCGGTACGTTGGGTCCGGTGGACCAAGCAGACCGTGACAAAGCAGACA
GTGACAAGACAAGCGCCGGA

Downstream 100 bases:

>100_bases
GTCATCCCGTCACCGCCTCGGTGAGCAACGAGGCGATGCGGTGGTGAATGGCATCGGTGTCGTCCTCGACGGCGACGGTG
GACAGGAACAGCGCCGCACC

Product: O-methyltransferase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MLSGVSETALLTLNGRAHQARHPKAIIDDPMAIRLVDSIDFDFDKFGRKGQEMALRSLAFDRAAIQYLDAHPSATVVALA
EGLQTSFWRLTSARPDLDFHWVTVDFEPIIALRERLLPHSDRISTLAQSALDFSWMDHVDTSNGVFITAEGLLMYLQPHE
SMRLITECARRFPGGQMIFDLPPVLVKKLAPNGMRASRRYRVPPMPFSLTPNQLADLAITVPGVRAVHDLPMPRGRGLLF
EKLYPAFWEFKPTKQMRGAYTLLEFG

Sequences:

>Translated_266_residues
MLSGVSETALLTLNGRAHQARHPKAIIDDPMAIRLVDSIDFDFDKFGRKGQEMALRSLAFDRAAIQYLDAHPSATVVALA
EGLQTSFWRLTSARPDLDFHWVTVDFEPIIALRERLLPHSDRISTLAQSALDFSWMDHVDTSNGVFITAEGLLMYLQPHE
SMRLITECARRFPGGQMIFDLPPVLVKKLAPNGMRASRRYRVPPMPFSLTPNQLADLAITVPGVRAVHDLPMPRGRGLLF
EKLYPAFWEFKPTKQMRGAYTLLEFG
>Mature_266_residues
MLSGVSETALLTLNGRAHQARHPKAIIDDPMAIRLVDSIDFDFDKFGRKGQEMALRSLAFDRAAIQYLDAHPSATVVALA
EGLQTSFWRLTSARPDLDFHWVTVDFEPIIALRERLLPHSDRISTLAQSALDFSWMDHVDTSNGVFITAEGLLMYLQPHE
SMRLITECARRFPGGQMIFDLPPVLVKKLAPNGMRASRRYRVPPMPFSLTPNQLADLAITVPGVRAVHDLPMPRGRGLLF
EKLYPAFWEFKPTKQMRGAYTLLEFG

Specific function: O-methyltransferase that catalyzes the methylation of the C-9 carboxy group of tetracenomycin E (TCM E) to yield TCM A2. Catalyzes as well the following side reactions:methylation of 8- O-methyl-TCM D3 to 9-carboxymethyl-8-O-methyl-TCM D3; and of TCM B3 t

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007213
- InterPro:   IPR016874 [H]

Pfam domain/function: PF04072 LCM [H]

EC number: NA

Molecular weight: Translated: 29988; Mature: 29988

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSGVSETALLTLNGRAHQARHPKAIIDDPMAIRLVDSIDFDFDKFGRKGQEMALRSLAF
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHCCCHHHHHHHHHHH
DRAAIQYLDAHPSATVVALAEGLQTSFWRLTSARPDLDFHWVTVDFEPIIALRERLLPHS
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHCCCC
DRISTLAQSALDFSWMDHVDTSNGVFITAEGLLMYLQPHESMRLITECARRFPGGQMIFD
HHHHHHHHHHHCCHHHHCCCCCCCEEEEECCEEEEECCCHHHHHHHHHHHHCCCCCEEEC
LPPVLVKKLAPNGMRASRRYRVPPMPFSLTPNQLADLAITVPGVRAVHDLPMPRGRGLLF
CCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCEEEECCCCHHHHHCCCCCCCCHHH
EKLYPAFWEFKPTKQMRGAYTLLEFG
HHHHHHHHCCCCHHHHCCEEEEEECC
>Mature Secondary Structure
MLSGVSETALLTLNGRAHQARHPKAIIDDPMAIRLVDSIDFDFDKFGRKGQEMALRSLAF
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHCCCHHHHHHHHHHH
DRAAIQYLDAHPSATVVALAEGLQTSFWRLTSARPDLDFHWVTVDFEPIIALRERLLPHS
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHCCCC
DRISTLAQSALDFSWMDHVDTSNGVFITAEGLLMYLQPHESMRLITECARRFPGGQMIFD
HHHHHHHHHHHCCHHHHCCCCCCCEEEEECCEEEEECCCHHHHHHHHHHHHCCCCCEEEC
LPPVLVKKLAPNGMRASRRYRVPPMPFSLTPNQLADLAITVPGVRAVHDLPMPRGRGLLF
CCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCEEEECCCCHHHHHCCCCCCCCHHH
EKLYPAFWEFKPTKQMRGAYTLLEFG
HHHHHHHHCCCCHHHHCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8509339 [H]