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The map label for this gene is rsbU [H]

Identifier: 119871274

GI number: 119871274

Start: 5485406

End: 5486701

Strand: Direct

Name: rsbU [H]

Synonym: Mkms_5247

Alternate gene names: 119871274

Gene position: 5485406-5486701 (Clockwise)

Preceding gene: 119871268

Following gene: 119871275

Centisome position: 95.61

GC content: 73.38

Gene sequence:

>1296_bases
ATGCCCAGGGAAACCGCGACGACCGTCCCGGATGAACCCGCCGCTCGCAGCCGGGCGCAGCTCACCGAGGACGTCGAGAT
CGGCCTGGCCGGGACCCTCAACCTGCGCCGGACCGGTCTGCGGCTACTCACCATGCTGCGGCCCGAACTCGCCGACTGGA
CGGCGCTCGTCCTGCCCGACAACCGCACCGGCGGGGTGACCTTCTTCGGCGGTGACCAGGTCGGCTTCACCGACCTCGTG
CCCCGCCGGCCGGAGGCCTACCCGCTACTGGAGCGGACGCTGCGCACCGGACAACCACACCGCGTCCGGCTCGAGTCGGC
CGACGCGGACCCCGGTGCCCTGGCGGACATCGTGCACCATCCGCGCCTGTGCGACGAGGTGGCCTCCCGCTCCCCCGCCG
ACGTCCTGGCGCTCGGCCTGACCGCCCGGGGCGCAACGCTGGGCGTGCTGCTGCTCGCGCGCGTCGGCGACCGGAGCTGG
GGCGGTGATGCCGACAATGACGACGTGGTGTTCGCGCGGCGTCTCGCGGCGCGGGCGGCGGTGGCTCTCGATTCGGCCAG
GCTCTACGAAGAGCGCGGCCGGATCGCCGCCGCGCTGCAACGGGGTCTGCGGCCCCCGGTGCTGCCGGAGGTCGAGGGCG
TGCGGGTGGCCGCCCGCTACCGTCCGACGGCCGAGCACCTCGAGATCGGGGGCGACTTCTACGACGTCCACGGCGCGGCC
GGCGATTGGCTGTTCGCCCTCGGCGACGTCTGCGGTAAGGGTGTGGAGGCGGCCGCGCTGACCGGCCGCACCCGGCAGAG
CATCCGGACCGCCGCGCACTTCGACCGGCGTCCGGAGGTGGTGCTCGAAGCGCTCAACTCCGTCCTGCACGAGGCGGAGT
CCGATCAGTTCGTCACCGTGGTGTGCGCCCGGGTGCGGCCGCAGCCGGACGGCCACGCCGACGTCGACCTCGCCGTCGCC
GGCCATCCCGCCCCGATCGTGTTGCGGGCCGACGGCCGGGTGGAACAGCTCGAGGTGTTCGGCACCGCCGCCGGGGTGCG
CGCCCAGACGCGTTACCGCCCCGCCTCCGTGCGACTCGACCGCGGCGACACCATGCTGATGTACACCGACGGGGTCGACG
AGGCACGCGGCGATGCCGGCTTCTACGGCGTGGACCGGTTGACGGCGTTCCTGCCGGCCTACGCCGGTGCCGCACCCGAC
GTGGTGTGCGAGGCGGTGGAACAGGACGTACTCGAATTCCTCGACGGTCAGCCCCACGACGACATCGCGCTGTTGGCGGT
GACGTGCGGGATCTAG

Upstream 100 bases:

>100_bases
CGACGGCAACCAGGCTAACCCACGTCCACCCGCGGCGATCACCGAGCGCAGTGGCACTACGGTGAACATCACCGCTATCG
GACGGGGAAGTCTGGAGACC

Downstream 100 bases:

>100_bases
AACAGGTGCTGGCCCGATACGAGGCCACGCTCGTCGCCGACGACGCGACGTCCGCCCGCGCGCTGGTCGAGGAACTGCTG
GCCGACGGGATCGATCCGGT

Product: putative GAF sensor protein

Products: NA

Alternate protein names: Sigma factor sigB regulation protein rsbU [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MPRETATTVPDEPAARSRAQLTEDVEIGLAGTLNLRRTGLRLLTMLRPELADWTALVLPDNRTGGVTFFGGDQVGFTDLV
PRRPEAYPLLERTLRTGQPHRVRLESADADPGALADIVHHPRLCDEVASRSPADVLALGLTARGATLGVLLLARVGDRSW
GGDADNDDVVFARRLAARAAVALDSARLYEERGRIAAALQRGLRPPVLPEVEGVRVAARYRPTAEHLEIGGDFYDVHGAA
GDWLFALGDVCGKGVEAAALTGRTRQSIRTAAHFDRRPEVVLEALNSVLHEAESDQFVTVVCARVRPQPDGHADVDLAVA
GHPAPIVLRADGRVEQLEVFGTAAGVRAQTRYRPASVRLDRGDTMLMYTDGVDEARGDAGFYGVDRLTAFLPAYAGAAPD
VVCEAVEQDVLEFLDGQPHDDIALLAVTCGI

Sequences:

>Translated_431_residues
MPRETATTVPDEPAARSRAQLTEDVEIGLAGTLNLRRTGLRLLTMLRPELADWTALVLPDNRTGGVTFFGGDQVGFTDLV
PRRPEAYPLLERTLRTGQPHRVRLESADADPGALADIVHHPRLCDEVASRSPADVLALGLTARGATLGVLLLARVGDRSW
GGDADNDDVVFARRLAARAAVALDSARLYEERGRIAAALQRGLRPPVLPEVEGVRVAARYRPTAEHLEIGGDFYDVHGAA
GDWLFALGDVCGKGVEAAALTGRTRQSIRTAAHFDRRPEVVLEALNSVLHEAESDQFVTVVCARVRPQPDGHADVDLAVA
GHPAPIVLRADGRVEQLEVFGTAAGVRAQTRYRPASVRLDRGDTMLMYTDGVDEARGDAGFYGVDRLTAFLPAYAGAAPD
VVCEAVEQDVLEFLDGQPHDDIALLAVTCGI
>Mature_430_residues
PRETATTVPDEPAARSRAQLTEDVEIGLAGTLNLRRTGLRLLTMLRPELADWTALVLPDNRTGGVTFFGGDQVGFTDLVP
RRPEAYPLLERTLRTGQPHRVRLESADADPGALADIVHHPRLCDEVASRSPADVLALGLTARGATLGVLLLARVGDRSWG
GDADNDDVVFARRLAARAAVALDSARLYEERGRIAAALQRGLRPPVLPEVEGVRVAARYRPTAEHLEIGGDFYDVHGAAG
DWLFALGDVCGKGVEAAALTGRTRQSIRTAAHFDRRPEVVLEALNSVLHEAESDQFVTVVCARVRPQPDGHADVDLAVAG
HPAPIVLRADGRVEQLEVFGTAAGVRAQTRYRPASVRLDRGDTMLMYTDGVDEARGDAGFYGVDRLTAFLPAYAGAAPDV
VCEAVEQDVLEFLDGQPHDDIALLAVTCGI

Specific function: Positive regulator of sigma-B activity. Dephosphorylates rsbV in response to environmental stress conveyed from the rsbXST module [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PP2C-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017944
- InterPro:   IPR001932
- InterPro:   IPR014787
- InterPro:   IPR010822
- ProDom:   PD685490 [H]

Pfam domain/function: PF08673 RsbU_N; PF07228 SpoIIE [H]

EC number: =3.1.3.3 [H]

Molecular weight: Translated: 46299; Mature: 46168

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRETATTVPDEPAARSRAQLTEDVEIGLAGTLNLRRTGLRLLTMLRPELADWTALVLPD
CCCCCCCCCCCCCCHHHHHHCCCCCEECEEEECHHHHHHHHHHHHHCHHHCCCEEEEECC
NRTGGVTFFGGDQVGFTDLVPRRPEAYPLLERTLRTGQPHRVRLESADADPGALADIVHH
CCCCCEEEECCCCCCHHHCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHC
PRLCDEVASRSPADVLALGLTARGATLGVLLLARVGDRSWGGDADNDDVVFARRLAARAA
CHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
VALDSARLYEERGRIAAALQRGLRPPVLPEVEGVRVAARYRPTAEHLEIGGDFYDVHGAA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCEEEECCCC
GDWLFALGDVCGKGVEAAALTGRTRQSIRTAAHFDRRPEVVLEALNSVLHEAESDQFVTV
CHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEE
VCARVRPQPDGHADVDLAVAGHPAPIVLRADGRVEQLEVFGTAAGVRAQTRYRPASVRLD
EEECCCCCCCCCCCEEEEEECCCCEEEEECCCCEEEEEEEHHHCCCCHHCCCCCCEEEEC
RGDTMLMYTDGVDEARGDAGFYGVDRLTAFLPAYAGAAPDVVCEAVEQDVLEFLDGQPHD
CCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
DIALLAVTCGI
CEEEEEEECCC
>Mature Secondary Structure 
PRETATTVPDEPAARSRAQLTEDVEIGLAGTLNLRRTGLRLLTMLRPELADWTALVLPD
CCCCCCCCCCCCCHHHHHHCCCCCEECEEEECHHHHHHHHHHHHHCHHHCCCEEEEECC
NRTGGVTFFGGDQVGFTDLVPRRPEAYPLLERTLRTGQPHRVRLESADADPGALADIVHH
CCCCCEEEECCCCCCHHHCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHC
PRLCDEVASRSPADVLALGLTARGATLGVLLLARVGDRSWGGDADNDDVVFARRLAARAA
CHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
VALDSARLYEERGRIAAALQRGLRPPVLPEVEGVRVAARYRPTAEHLEIGGDFYDVHGAA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCEEEECCCC
GDWLFALGDVCGKGVEAAALTGRTRQSIRTAAHFDRRPEVVLEALNSVLHEAESDQFVTV
CHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEE
VCARVRPQPDGHADVDLAVAGHPAPIVLRADGRVEQLEVFGTAAGVRAQTRYRPASVRLD
EEECCCCCCCCCCCEEEEEECCCCEEEEECCCCEEEEEEEHHHCCCCHHCCCCCCEEEEC
RGDTMLMYTDGVDEARGDAGFYGVDRLTAFLPAYAGAAPDVVCEAVEQDVLEFLDGQPHD
CCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
DIALLAVTCGI
CEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8002610; 8002609; 9384377; 8824586; 9786195; 12499568 [H]