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The map label for this gene is glgX [H]

Identifier: 119871231

GI number: 119871231

Start: 5446100

End: 5448244

Strand: Direct

Name: glgX [H]

Synonym: Mkms_5204

Alternate gene names: 119871231

Gene position: 5446100-5448244 (Clockwise)

Preceding gene: 119871230

Following gene: 119871233

Centisome position: 94.93

GC content: 66.01

Gene sequence:

>2145_bases
TTGGCCGCAGCCCCCAAACCGAACGTCACCCCCGAAGTATGGCCCGGTAAGGCATACCCCCTCGGAGCGACGTATGACGG
CTTTGGCACCAATTTCGCGTTGTTCAGCGAGGCCGCCGAGCGTGTGGAGCTGTGCCTGTTCGACGCCGACGGCGAGCAGA
CGGCGTGTGTCACCCTGCCCGAGGTCGACGGCTTCGTCTGGCACGGGTTCATCCCGAACATCGAGCCGGGTCAGCGCTAC
GGCTACCGCGTGCACGGCCCGTACGATCCGCCCAACGGTCAGCGGTGCAATCCGAACAAGCTGCTGCTCGACCCGTACGC
CAAGGCCATCGACGGCACCTTCGACTGGGGCCAGCCGCTGTTCAGCTACAACTTCGGCGATCCGGACAGCCGCAACGACG
ACGACTCGGCGGATTGCATGCCGAAGTCGGTGGTGATCAACCCGTACTTCGACTGGGGCACCGACCGCCCGCCGAACCAT
GAGTACGCCGACACGGTGATCTACGAGGCCCACGTCAAGGGTCTGACGCAGACCCACCCCGACATCCCCGAGCAGATCCG
CGGCACCTACTCGGCGGTGGCCCACCCGGTGATCATCGACCACCTCAAGGCGCTCGGTGTCAACGCGATCGAGCTGATGC
CGGTGCACCACTTCGCCAACGACTCGACGCTGCTCGACAAGGGGCTGTCGAACTACTGGGGCTACAACACGATCGGGTTC
CTCGCCCCCGATCCCAAGTACAGCTCGAGCCTCACCCCCGGTGGCCAGGTGCAGGAGTTCAAGGCGATGGTGCGCACCCT
GCACGAGGCGGGGATCGAGGTCATCCTCGACGTGGTCTACAACCACACCGCCGAGGGCAACCACATGGGGCCGACGCTGT
CCTTCCGCGGCATCGACAACGCCGCGTACTACCGGCTCGTCGACGACGACAAGCGCTACTACATGGACTACACCGGCACC
GGCAACAGCCTCAACGCCGGCCACCCGCACGCCCTGCAGCTCATCATGGACTCGCTGCGGTACTGGGTGACCGAGATGCA
CGTCGACGGTTTCCGGTTCGATCTGGCCTCGACGCTGGCCCGCGAGTTCTACGACGTCGACCGGCTGGCGACGTTCTTCG
AACTCGTGCAGCAGGATCCGACCGTCAGCCAGGTCAAGCTGATCGCTGAACCGTGGGACGTCGGCCCGGGCGGGTACCAG
GTCGGCAACTTCCCGCCGCAGTGGACGGAGTGGAACGGCAAGTACCGCGACACCGTGCGCGACTACTGGCGCGGCGAATC
GGCGACGCTGGACGAATTCGCCTCGAGGCTGACCGGGTCGTCGGACCTCTACGAGCACACCTCGCGCAGGCCCGTCGCGT
CGATCAACTTCGTCATCGCCCACGACGGCTTCACGCTGCGGGACCTGGTGTCCTACAACGAGAAACACAACGAGGACAAC
GGCGAGGACAACAACGACGGGGAGAGCCACAACCGGTCGTGGAACTGCGGTGTGGAAGGCCCGACGGACGATCCTCAGGT
CAACGCGCTACGCAGCCGTCAGCAGCGCAACTTCCTCACCACCCTGCTGCTGAGCCAGGGCGTGCCGATGATCGCGCACG
GCGACGAGCTCGGGCGCACGCAGCACGGCAACAACAACGTCTACTGCCAGGACAACGAACTGTCCTGGATCGACTGGTCG
ACCGTCGACACCGAGTTGATGGCGTTCACCCAGAAGGTCTCGGAACTGCGCGCCGCACACCCGGTGTTCCGTCGGCGCCG
GTTCTTCTCCGGCCGTCCGGTCCGCCAGCGCGGTGCGCCCGGCCTCCCCGACATCGCCTGGTTCGCCCCCGACGGCTCGG
AGATGACCGACGAGGACTGGGACAGCGGCTTCGCCAAGTCGATCGCGGTCTACCTCAACGGCCAGGGCATCCCCGATCTG
GACGTCCGCGGTCAGCGCGTCACCGACGACTCGTTCCTGCTGTGTTTCAACGCCCACCACGAGCCGCTCGAATTCGCCAT
GCCCGCAGCGGAATTCGCCCGGGCCTGGCTCCCGGTGATCAACACCGCAGACGACGATGACGCCCGCGAGTCGGTGACTG
CGGGCGCCAAGATCGAGGTCGCCGAACGGTCGGTGCTGGTGCTGCAGGCCGTCAACGAGCCCTGA

Upstream 100 bases:

>100_bases
ATCTCCGTGACGCCGCTACGGGCGCCGGCCGTGCTGCCCGCACCCCGGTCCGCCCGTATCGTCGCCCTCGTCCGTACCCC
GAAGCTCAGGAGCGTGCACT

Downstream 100 bases:

>100_bases
AGTGGGTCACTCGACGACCGTGCTCATTCAACGACGGTGAAGAACGAGTCACCGTCCTCCGGGGTGCCGCTGACCTGACC
GGCATCGGTGCCGTGATGGT

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 714; Mature: 713

Protein sequence:

>714_residues
MAAAPKPNVTPEVWPGKAYPLGATYDGFGTNFALFSEAAERVELCLFDADGEQTACVTLPEVDGFVWHGFIPNIEPGQRY
GYRVHGPYDPPNGQRCNPNKLLLDPYAKAIDGTFDWGQPLFSYNFGDPDSRNDDDSADCMPKSVVINPYFDWGTDRPPNH
EYADTVIYEAHVKGLTQTHPDIPEQIRGTYSAVAHPVIIDHLKALGVNAIELMPVHHFANDSTLLDKGLSNYWGYNTIGF
LAPDPKYSSSLTPGGQVQEFKAMVRTLHEAGIEVILDVVYNHTAEGNHMGPTLSFRGIDNAAYYRLVDDDKRYYMDYTGT
GNSLNAGHPHALQLIMDSLRYWVTEMHVDGFRFDLASTLAREFYDVDRLATFFELVQQDPTVSQVKLIAEPWDVGPGGYQ
VGNFPPQWTEWNGKYRDTVRDYWRGESATLDEFASRLTGSSDLYEHTSRRPVASINFVIAHDGFTLRDLVSYNEKHNEDN
GEDNNDGESHNRSWNCGVEGPTDDPQVNALRSRQQRNFLTTLLLSQGVPMIAHGDELGRTQHGNNNVYCQDNELSWIDWS
TVDTELMAFTQKVSELRAAHPVFRRRRFFSGRPVRQRGAPGLPDIAWFAPDGSEMTDEDWDSGFAKSIAVYLNGQGIPDL
DVRGQRVTDDSFLLCFNAHHEPLEFAMPAAEFARAWLPVINTADDDDARESVTAGAKIEVAERSVLVLQAVNEP

Sequences:

>Translated_714_residues
MAAAPKPNVTPEVWPGKAYPLGATYDGFGTNFALFSEAAERVELCLFDADGEQTACVTLPEVDGFVWHGFIPNIEPGQRY
GYRVHGPYDPPNGQRCNPNKLLLDPYAKAIDGTFDWGQPLFSYNFGDPDSRNDDDSADCMPKSVVINPYFDWGTDRPPNH
EYADTVIYEAHVKGLTQTHPDIPEQIRGTYSAVAHPVIIDHLKALGVNAIELMPVHHFANDSTLLDKGLSNYWGYNTIGF
LAPDPKYSSSLTPGGQVQEFKAMVRTLHEAGIEVILDVVYNHTAEGNHMGPTLSFRGIDNAAYYRLVDDDKRYYMDYTGT
GNSLNAGHPHALQLIMDSLRYWVTEMHVDGFRFDLASTLAREFYDVDRLATFFELVQQDPTVSQVKLIAEPWDVGPGGYQ
VGNFPPQWTEWNGKYRDTVRDYWRGESATLDEFASRLTGSSDLYEHTSRRPVASINFVIAHDGFTLRDLVSYNEKHNEDN
GEDNNDGESHNRSWNCGVEGPTDDPQVNALRSRQQRNFLTTLLLSQGVPMIAHGDELGRTQHGNNNVYCQDNELSWIDWS
TVDTELMAFTQKVSELRAAHPVFRRRRFFSGRPVRQRGAPGLPDIAWFAPDGSEMTDEDWDSGFAKSIAVYLNGQGIPDL
DVRGQRVTDDSFLLCFNAHHEPLEFAMPAAEFARAWLPVINTADDDDARESVTAGAKIEVAERSVLVLQAVNEP
>Mature_713_residues
AAAPKPNVTPEVWPGKAYPLGATYDGFGTNFALFSEAAERVELCLFDADGEQTACVTLPEVDGFVWHGFIPNIEPGQRYG
YRVHGPYDPPNGQRCNPNKLLLDPYAKAIDGTFDWGQPLFSYNFGDPDSRNDDDSADCMPKSVVINPYFDWGTDRPPNHE
YADTVIYEAHVKGLTQTHPDIPEQIRGTYSAVAHPVIIDHLKALGVNAIELMPVHHFANDSTLLDKGLSNYWGYNTIGFL
APDPKYSSSLTPGGQVQEFKAMVRTLHEAGIEVILDVVYNHTAEGNHMGPTLSFRGIDNAAYYRLVDDDKRYYMDYTGTG
NSLNAGHPHALQLIMDSLRYWVTEMHVDGFRFDLASTLAREFYDVDRLATFFELVQQDPTVSQVKLIAEPWDVGPGGYQV
GNFPPQWTEWNGKYRDTVRDYWRGESATLDEFASRLTGSSDLYEHTSRRPVASINFVIAHDGFTLRDLVSYNEKHNEDNG
EDNNDGESHNRSWNCGVEGPTDDPQVNALRSRQQRNFLTTLLLSQGVPMIAHGDELGRTQHGNNNVYCQDNELSWIDWST
VDTELMAFTQKVSELRAAHPVFRRRRFFSGRPVRQRGAPGLPDIAWFAPDGSEMTDEDWDSGFAKSIAVYLNGQGIPDLD
VRGQRVTDDSFLLCFNAHHEPLEFAMPAAEFARAWLPVINTADDDDARESVTAGAKIEVAERSVLVLQAVNEP

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=657, Percent_Identity=46.8797564687976, Blast_Score=582, Evalue=1e-167,
Organism=Saccharomyces cerevisiae, GI6320826, Length=214, Percent_Identity=27.1028037383178, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 79990; Mature: 79859

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAAPKPNVTPEVWPGKAYPLGATYDGFGTNFALFSEAAERVELCLFDADGEQTACVTLP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEEEECCCCCEEEEECC
EVDGFVWHGFIPNIEPGQRYGYRVHGPYDPPNGQRCNPNKLLLDPYAKAIDGTFDWGQPL
CCCCEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCHHHHHCCCCCCCCCC
FSYNFGDPDSRNDDDSADCMPKSVVINPYFDWGTDRPPNHEYADTVIYEAHVKGLTQTHP
EECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHEEEEHHHCCCCCCCC
DIPEQIRGTYSAVAHPVIIDHLKALGVNAIELMPVHHFANDSTLLDKGLSNYWGYNTIGF
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHCCCCCHHHHHHHHHHCCCCEEEE
LAPDPKYSSSLTPGGQVQEFKAMVRTLHEAGIEVILDVVYNHTAEGNHMGPTLSFRGIDN
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCC
AAYYRLVDDDKRYYMDYTGTGNSLNAGHPHALQLIMDSLRYWVTEMHVDGFRFDLASTLA
CEEEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
REFYDVDRLATFFELVQQDPTVSQVKLIAEPWDVGPGGYQVGNFPPQWTEWNGKYRDTVR
HHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
DYWRGESATLDEFASRLTGSSDLYEHTSRRPVASINFVIAHDGFTLRDLVSYNEKHNEDN
HHHCCCCCCHHHHHHHHCCCHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCC
GEDNNDGESHNRSWNCGVEGPTDDPQVNALRSRQQRNFLTTLLLSQGVPMIAHGDELGRT
CCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHCCC
QHGNNNVYCQDNELSWIDWSTVDTELMAFTQKVSELRAAHPVFRRRRFFSGRPVRQRGAP
CCCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCC
GLPDIAWFAPDGSEMTDEDWDSGFAKSIAVYLNGQGIPDLDVRGQRVTDDSFLLCFNAHH
CCCCEEEECCCCCCCCCCHHCCCCCEEEEEEECCCCCCCCCCCCCEECCCCEEEEECCCC
EPLEFAMPAAEFARAWLPVINTADDDDARESVTAGAKIEVAERSVLVLQAVNEP
CCHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEECCCEEEEEEECCCC
>Mature Secondary Structure 
AAAPKPNVTPEVWPGKAYPLGATYDGFGTNFALFSEAAERVELCLFDADGEQTACVTLP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEEEECCCCCEEEEECC
EVDGFVWHGFIPNIEPGQRYGYRVHGPYDPPNGQRCNPNKLLLDPYAKAIDGTFDWGQPL
CCCCEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCHHHHHCCCCCCCCCC
FSYNFGDPDSRNDDDSADCMPKSVVINPYFDWGTDRPPNHEYADTVIYEAHVKGLTQTHP
EECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHEEEEHHHCCCCCCCC
DIPEQIRGTYSAVAHPVIIDHLKALGVNAIELMPVHHFANDSTLLDKGLSNYWGYNTIGF
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHCCCCCHHHHHHHHHHCCCCEEEE
LAPDPKYSSSLTPGGQVQEFKAMVRTLHEAGIEVILDVVYNHTAEGNHMGPTLSFRGIDN
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCC
AAYYRLVDDDKRYYMDYTGTGNSLNAGHPHALQLIMDSLRYWVTEMHVDGFRFDLASTLA
CEEEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
REFYDVDRLATFFELVQQDPTVSQVKLIAEPWDVGPGGYQVGNFPPQWTEWNGKYRDTVR
HHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
DYWRGESATLDEFASRLTGSSDLYEHTSRRPVASINFVIAHDGFTLRDLVSYNEKHNEDN
HHHCCCCCCHHHHHHHHCCCHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCC
GEDNNDGESHNRSWNCGVEGPTDDPQVNALRSRQQRNFLTTLLLSQGVPMIAHGDELGRT
CCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHCCC
QHGNNNVYCQDNELSWIDWSTVDTELMAFTQKVSELRAAHPVFRRRRFFSGRPVRQRGAP
CCCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCC
GLPDIAWFAPDGSEMTDEDWDSGFAKSIAVYLNGQGIPDLDVRGQRVTDDSFLLCFNAHH
CCCCEEEECCCCCCCCCCHHCCCCCEEEEEEECCCCCCCCCCCCCEECCCCEEEEECCCC
EPLEFAMPAAEFARAWLPVINTADDDDARESVTAGAKIEVAERSVLVLQAVNEP
CCHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEECCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]