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The map label for this gene is pncA [H]

Identifier: 119871230

GI number: 119871230

Start: 5445392

End: 5445946

Strand: Direct

Name: pncA [H]

Synonym: Mkms_5203

Alternate gene names: 119871230

Gene position: 5445392-5445946 (Clockwise)

Preceding gene: 119871229

Following gene: 119871231

Centisome position: 94.91

GC content: 72.07

Gene sequence:

>555_bases
ATGCGCGCGCTCATCGTCGTCGACGTCCAGAACGACTTCTGCGAAGGCGGCTCGCTGGCCGTGACCGGCGGGGCCGCCGT
CGCCCGGCGGATCAGCGACCTCCTCGCGGACGGCACCGCTCGCTACGACCACATCGTGGCGACCAAGGACTTCCACATCG
ACCCGGGCGAGCACTTCTCCGATACGCCCGACTATCGGGTGTCGTGGCCCCGTCACTGTGTCGTCGGCACCGACGGCGCG
GAGTTCCACCCCGACCTGGATCCGTCCCCGGTCGAGGCGGTGTTCACCAAGGGTGAGTACTCGGCGGCCTACAGCGGCTT
CGAGGGCAGCGACGCGTCGGGCACGCCGCTGGGTGACTGGCTGCGCGAGCGCGGCGTCGACGAGGTCGACGTGGTCGGGA
TCGCCACCGACTACTGCGTCCGGGCCACCGCGGCGGATGCGGCGGCGGCCGGTTTCACCACCCGGGTGCTGCTCGACCTC
ACCGCGGGTGTCGCCGAGGACTCGACCGCCGACGCGGTCCGAGCGCTGCGCGACGCCGGCGTCGCCGTCGTCTGA

Upstream 100 bases:

>100_bases
GCTGCCGCCGCGCTCGGTCTTCGACCACCTGCTGCTCACGGTCCACCATCGGGTCCGCGCGGCAGGTGTGTGACGCGCCG
CGCGGCGCTACCGTGACGTC

Downstream 100 bases:

>100_bases
GGCGGCCCGGCGGTGTGACGGGACACGCCGTTGAGAAACGGTGAAGTGGGTATATCTCCGTGACGCCGCTACGGGCGCCG
GCCGTGCTGCCCGCACCCCG

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MRALIVVDVQNDFCEGGSLAVTGGAAVARRISDLLADGTARYDHIVATKDFHIDPGEHFSDTPDYRVSWPRHCVVGTDGA
EFHPDLDPSPVEAVFTKGEYSAAYSGFEGSDASGTPLGDWLRERGVDEVDVVGIATDYCVRATAADAAAAGFTTRVLLDL
TAGVAEDSTADAVRALRDAGVAVV

Sequences:

>Translated_184_residues
MRALIVVDVQNDFCEGGSLAVTGGAAVARRISDLLADGTARYDHIVATKDFHIDPGEHFSDTPDYRVSWPRHCVVGTDGA
EFHPDLDPSPVEAVFTKGEYSAAYSGFEGSDASGTPLGDWLRERGVDEVDVVGIATDYCVRATAADAAAAGFTTRVLLDL
TAGVAEDSTADAVRALRDAGVAVV
>Mature_184_residues
MRALIVVDVQNDFCEGGSLAVTGGAAVARRISDLLADGTARYDHIVATKDFHIDPGEHFSDTPDYRVSWPRHCVVGTDGA
EFHPDLDPSPVEAVFTKGEYSAAYSGFEGSDASGTPLGDWLRERGVDEVDVVGIATDYCVRATAADAAAAGFTTRVLLDL
TAGVAEDSTADAVRALRDAGVAVV

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=180, Percent_Identity=37.7777777777778, Blast_Score=114, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17543130, Length=222, Percent_Identity=28.3783783783784, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI193206735, Length=222, Percent_Identity=28.3783783783784, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321401, Length=199, Percent_Identity=32.1608040201005, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 19303; Mature: 19303

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALIVVDVQNDFCEGGSLAVTGGAAVARRISDLLADGTARYDHIVATKDFHIDPGEHFS
CCEEEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCC
DTPDYRVSWPRHCVVGTDGAEFHPDLDPSPVEAVFTKGEYSAAYSGFEGSDASGTPLGDW
CCCCCEECCCCEEEEECCCCCCCCCCCCCCHHEEEECCCCHHHCCCCCCCCCCCCCHHHH
LRERGVDEVDVVGIATDYCVRATAADAAAAGFTTRVLLDLTAGVAEDSTADAVRALRDAG
HHHCCCCCEEEEEEEHHHHHHHHHCHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
VAVV
CCCC
>Mature Secondary Structure
MRALIVVDVQNDFCEGGSLAVTGGAAVARRISDLLADGTARYDHIVATKDFHIDPGEHFS
CCEEEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCC
DTPDYRVSWPRHCVVGTDGAEFHPDLDPSPVEAVFTKGEYSAAYSGFEGSDASGTPLGDW
CCCCCEECCCCEEEEECCCCCCCCCCCCCCHHEEEECCCCHHHCCCCCCCCCCCCCHHHH
LRERGVDEVDVVGIATDYCVRATAADAAAAGFTTRVLLDLTAGVAEDSTADAVRALRDAG
HHHCCCCCEEEEEEEHHHHHHHHHCHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
VAVV
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]