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The map label for this gene is rfbE [H]

Identifier: 119871106

GI number: 119871106

Start: 5315015

End: 5315836

Strand: Direct

Name: rfbE [H]

Synonym: Mkms_5079

Alternate gene names: 119871106

Gene position: 5315015-5315836 (Clockwise)

Preceding gene: 119871105

Following gene: 119871107

Centisome position: 92.64

GC content: 64.96

Gene sequence:

>822_bases
GTGGCGGTTCCTCGTATCGAGACCCGTGACGCATGGGTCGAGTTCCCGATCTTCGATGCCAAGACCCGGTCGCTGAAGAA
GGCGTTCCTCGGAAAGGCCGGCGGGGCCATCGGGCGCAACGACTCGAACGTCGTCGTCATCGAAGCCCTCCGCGACATCA
CCATGTCGCTGGAACTCGGGGACCGGGTCGGTCTGGTCGGCCACAACGGCGCAGGTAAATCGACACTGCTGCGGTTGCTG
TCGGGCATCTACGAGCCGACCCGGGGGTCTGCCACCGTGACGGGCCGGGTGGCGCCGGTGTTCGACCTCGGCGTCGGGAT
GGATCCGGAGATCTCCGGCTACGAGAACATCATCATCCGCGGACTGTTCCTCGGTCAGACGCGTAAGCAGATGCTGGCCA
AGGTCGACGAGATCGCGGAGTTCACCGAACTCGGTGACTACCTGTCGATGCCGCTGCGCACGTACTCCACCGGTATGCGG
GTGCGGTTGGCGATGGGTGTGGTCACCAGCATCGACCCGGAGATCCTGCTGCTCGACGAGGGTATCGGGGCGGTGGACGC
CGACTTCCTCAAGAAGGCGCAGTCGCGACTGCAGAGCCTGGTCGAACGGTCCGGGATCCTGGTGTTCGCCAGCCACTCCA
ACGAATTCCTCGCCCGGTTGTGCAAGACCGCGATGTGGATCGACCACGGCACCGTCAAGATGACCGGCGGTATCGAGGAC
GTCGTGCGGGCCTACGAGGGTGAGGACGCCGCGCGGCATGTGCGAGAAGTGCTCGACGAACACCGCGGGGACTGGTCAGC
CACCGACGAGCCTTCCCGATGA

Upstream 100 bases:

>100_bases
GAACAGATGCGGCAGGGTGCGTTGCCCCCCGGCATCGCGGTGCAGGGCCAGCGCGGCGGACCCGCGCACCCCGGCACCGG
TCAGTACCTGTAGGGCAGCG

Downstream 100 bases:

>100_bases
CAGACAGGATCTACGTCGTCGTCGTGACCCACCGGCGTCCGGAGTCGCTGGCGCAATCGCTCGACGCGCTGTGCGCGCAG
ACGCGCCGGCCGGACGGGCT

Product: ABC transporter-like protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MAVPRIETRDAWVEFPIFDAKTRSLKKAFLGKAGGAIGRNDSNVVVIEALRDITMSLELGDRVGLVGHNGAGKSTLLRLL
SGIYEPTRGSATVTGRVAPVFDLGVGMDPEISGYENIIIRGLFLGQTRKQMLAKVDEIAEFTELGDYLSMPLRTYSTGMR
VRLAMGVVTSIDPEILLLDEGIGAVDADFLKKAQSRLQSLVERSGILVFASHSNEFLARLCKTAMWIDHGTVKMTGGIED
VVRAYEGEDAARHVREVLDEHRGDWSATDEPSR

Sequences:

>Translated_273_residues
MAVPRIETRDAWVEFPIFDAKTRSLKKAFLGKAGGAIGRNDSNVVVIEALRDITMSLELGDRVGLVGHNGAGKSTLLRLL
SGIYEPTRGSATVTGRVAPVFDLGVGMDPEISGYENIIIRGLFLGQTRKQMLAKVDEIAEFTELGDYLSMPLRTYSTGMR
VRLAMGVVTSIDPEILLLDEGIGAVDADFLKKAQSRLQSLVERSGILVFASHSNEFLARLCKTAMWIDHGTVKMTGGIED
VVRAYEGEDAARHVREVLDEHRGDWSATDEPSR
>Mature_272_residues
AVPRIETRDAWVEFPIFDAKTRSLKKAFLGKAGGAIGRNDSNVVVIEALRDITMSLELGDRVGLVGHNGAGKSTLLRLLS
GIYEPTRGSATVTGRVAPVFDLGVGMDPEISGYENIIIRGLFLGQTRKQMLAKVDEIAEFTELGDYLSMPLRTYSTGMRV
RLAMGVVTSIDPEILLLDEGIGAVDADFLKKAQSRLQSLVERSGILVFASHSNEFLARLCKTAMWIDHGTVKMTGGIEDV
VRAYEGEDAARHVREVLDEHRGDWSATDEPSR

Specific function: May form an ATP-driven O-antigen export apparatus, in association with RfbD [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI153792144, Length=206, Percent_Identity=25.7281553398058, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI27477115, Length=172, Percent_Identity=24.4186046511628, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI31657092, Length=210, Percent_Identity=26.1904761904762, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI27436953, Length=235, Percent_Identity=25.9574468085106, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI116734710, Length=168, Percent_Identity=27.3809523809524, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1790467, Length=198, Percent_Identity=28.7878787878788, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788761, Length=230, Percent_Identity=27.3913043478261, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87081791, Length=226, Percent_Identity=25.2212389380531, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI87081782, Length=214, Percent_Identity=29.4392523364486, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI87081709, Length=217, Percent_Identity=23.963133640553, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082267, Length=201, Percent_Identity=26.865671641791, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1789751, Length=178, Percent_Identity=29.2134831460674, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787089, Length=211, Percent_Identity=26.0663507109005, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786319, Length=195, Percent_Identity=26.1538461538462, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787712, Length=177, Percent_Identity=27.1186440677966, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787758, Length=215, Percent_Identity=27.4418604651163, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI48995001, Length=182, Percent_Identity=26.9230769230769, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1788450, Length=225, Percent_Identity=26.2222222222222, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787041, Length=202, Percent_Identity=25.7425742574257, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789032, Length=239, Percent_Identity=24.2677824267782, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI48994943, Length=178, Percent_Identity=28.6516853932584, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788225, Length=211, Percent_Identity=25.1184834123223, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI2367384, Length=189, Percent_Identity=28.5714285714286, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17565586, Length=213, Percent_Identity=26.2910798122066, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28573571, Length=233, Percent_Identity=25.7510729613734, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI28573573, Length=233, Percent_Identity=25.7510729613734, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: 3.6.3.25 [C]

Molecular weight: Translated: 29865; Mature: 29733

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVPRIETRDAWVEFPIFDAKTRSLKKAFLGKAGGAIGRNDSNVVVIEALRDITMSLELG
CCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHEEEECC
DRVGLVGHNGAGKSTLLRLLSGIYEPTRGSATVTGRVAPVFDLGVGMDPEISGYENIIIR
CEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCEECCCCCCCCCCCCCCEEEE
GLFLGQTRKQMLAKVDEIAEFTELGDYLSMPLRTYSTGMRVRLAMGVVTSIDPEILLLDE
EEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEHHHHHCCCCCEEEEEC
GIGAVDADFLKKAQSRLQSLVERSGILVFASHSNEFLARLCKTAMWIDHGTVKMTGGIED
CCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCHHH
VVRAYEGEDAARHVREVLDEHRGDWSATDEPSR
HHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AVPRIETRDAWVEFPIFDAKTRSLKKAFLGKAGGAIGRNDSNVVVIEALRDITMSLELG
CCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHEEEECC
DRVGLVGHNGAGKSTLLRLLSGIYEPTRGSATVTGRVAPVFDLGVGMDPEISGYENIIIR
CEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCEECCCCCCCCCCCCCCEEEE
GLFLGQTRKQMLAKVDEIAEFTELGDYLSMPLRTYSTGMRVRLAMGVVTSIDPEILLLDE
EEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEHHHHHCCCCCEEEEEC
GIGAVDADFLKKAQSRLQSLVERSGILVFASHSNEFLARLCKTAMWIDHGTVKMTGGIED
CCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCHHH
VVRAYEGEDAARHVREVLDEHRGDWSATDEPSR
HHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7692217 [H]