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The map label for this gene is leuA [H]

Identifier: 119871014

GI number: 119871014

Start: 5226568

End: 5228373

Strand: Direct

Name: leuA [H]

Synonym: Mkms_4987

Alternate gene names: 119871014

Gene position: 5226568-5228373 (Clockwise)

Preceding gene: 119871013

Following gene: 119871015

Centisome position: 91.1

GC content: 67.94

Gene sequence:

>1806_bases
ATGAACACCCCTGAATCAACAGACGCCTTCTCGTCCGTCCGCGCCATCACCACCCCGTCCGGCCCGCCGCATCCCGGCCA
GCCGGCCTGGAACACCCAACGCGCCTCCGCCATGCCGGTCAGCCGGTACCGCAGCTTCGCCGAGGAAGTGGAACCGGTCA
CGCTGCCGGACCGCACCTGGCCCGACAAGGTCGTCGACACCGCGCCGATGTGGTGCGCGGTCGACCTGCGCGACGGCAAC
CAGGCGCTGATCGACCCGATGAGCCCCGCGCGCAAACGCCGCATGTTCGACCTGCTGGTGCGCATGGGCTACAAGGAGAT
CGAGGTCGGCTTCCCGTCGGCCAGCCAGACCGATTTCGACTTCGTCCGCGAGATCATCGAACAGGGCGCGATCCCCGACG
ACGTCACCATCCAGGTGCTGACGCAGTGCCGGCCCGAACTGATCACCCGCACCTTCGAGGCCTGCAAGGGCGCACCGCGG
GCGATCGTGCACTTCTACAACTCGACGTCGATCCTGCAGCGCCGCGTCGTGTTCCGCGCCGACCGCGAGGCCGTCAAGCA
GATCGCCGTCGACGGCGCCCGGATGTGCGTCGAAGAGGCCGCCAAGCACCCCGACACGCTGTGGCGGTTCGAGTACTCCC
CCGAGTCCTACACCGGCACCGAACTGGAATACGCCGTCGACGTCTGCAACGCGGTCGCCGACATCGTGAAGCCCACCCCC
GAGGTGCCGCTGATCGTCAACCTGCCCGCCACCGTCGAGATGGCGACGCCCAACGTCTACGCCGACTCGATCGAGTGGAT
GAACCGGCACCTGAGCCCGCGCGACAGCATCATCCTGAGCCTGCACCCGCACAACGACCGCGGAACCGCTGTCGCCGCAG
CGGAATTGGGCTACCAGGCCGGTGCCGACCGCATCGAGGGCTGCCTGTTCGGCAACGGTGAGCGCACCGGCAACGTATGC
CTGGTGACGCTGGGCCTGAACCTGTTCAGCCGGGGCGTCGACCCGCAGATCGACTTCTCCAACATCGACGAGATCCGCCG
CACCGTCGAGTACTGCAACCAGCTGCCGGTCCACGAGCGTCACCCCTACGGCGGCGACCTGGTGTACACCGCGTTCTCCG
GCAGCCATCAGGACGCGATCAACAAGGGTCTGGACGCGATGAAGGTCGCCGCGGACGAAGCGGACTCCGACGTCGACGAC
ATCCTGTGGCAGGTGCCGTATCTGCCGATCGATCCGAAGGACGTCGGCCGCACCTACGAAGCCGTCATCCGGGTGAACTC
CCAGTCCGGCAAGGGCGGCGTCGCCTACATCATGAAGGCCGACCACGGACTGGTGCTGCCGCGCCGCCTGCAGATCGAGT
TCAGCCAGGCCATCCAGAAGATCACCGACGGTGAGGGCGGCGAGGTGTCGCCCAAGGAGATGTGGGACGTCTTCGCCGAG
GAGTACCTGTCGCCGGTCCGGCCGCTGGAGCGGATGCGCCAGAAGGTCGACGCCGCGGAGGTCGACGGCGGCACCGACAC
GATCACCGCCGTGGTGAAGGTGGACGGTGTCGAACGCGAGATCGTCGGCGCGGGCAACGGGCCGTTGGCCGCGTTCTGCG
ACGCCCTGGGCGCGATCGGATTCGACATCAGCGTCCTGGACTACTCCGAGCATGCACTGTCCGCCGGTGAGGAGGCGCAG
GCCGCGGCCTACGTCGAGGCCTCCGTCGGCGGTAAGACGGTATGGGGTGTGGGCATCGCGACCTCGATCACGACGGCCTC
GCTGCGCGCGGTGGTGTCGGCGGTGAACCGCGCCGCACGCAGCTGA

Upstream 100 bases:

>100_bases
TCTCGGCCGCCGCGGCGGGGTCTGACCAGACCGGCTTCCCGTCGCGGGTGTTCGCGATGCGCCGGTCGAGTCCCTCGTCA
AACTCCCGGAGCAATCGATC

Downstream 100 bases:

>100_bases
CCGTGAGAGGCTGCCCAGGTGACTGTCGTCGTCGTGGGGAATCCGAAACCTGAGTCGCGCACGCGGGCCGCCGCCGAACT
GGTCGCCGAGAGGCTGACCG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MNTPESTDAFSSVRAITTPSGPPHPGQPAWNTQRASAMPVSRYRSFAEEVEPVTLPDRTWPDKVVDTAPMWCAVDLRDGN
QALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQTDFDFVREIIEQGAIPDDVTIQVLTQCRPELITRTFEACKGAPR
AIVHFYNSTSILQRRVVFRADREAVKQIAVDGARMCVEEAAKHPDTLWRFEYSPESYTGTELEYAVDVCNAVADIVKPTP
EVPLIVNLPATVEMATPNVYADSIEWMNRHLSPRDSIILSLHPHNDRGTAVAAAELGYQAGADRIEGCLFGNGERTGNVC
LVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPVHERHPYGGDLVYTAFSGSHQDAINKGLDAMKVAADEADSDVDD
ILWQVPYLPIDPKDVGRTYEAVIRVNSQSGKGGVAYIMKADHGLVLPRRLQIEFSQAIQKITDGEGGEVSPKEMWDVFAE
EYLSPVRPLERMRQKVDAAEVDGGTDTITAVVKVDGVEREIVGAGNGPLAAFCDALGAIGFDISVLDYSEHALSAGEEAQ
AAAYVEASVGGKTVWGVGIATSITTASLRAVVSAVNRAARS

Sequences:

>Translated_601_residues
MNTPESTDAFSSVRAITTPSGPPHPGQPAWNTQRASAMPVSRYRSFAEEVEPVTLPDRTWPDKVVDTAPMWCAVDLRDGN
QALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQTDFDFVREIIEQGAIPDDVTIQVLTQCRPELITRTFEACKGAPR
AIVHFYNSTSILQRRVVFRADREAVKQIAVDGARMCVEEAAKHPDTLWRFEYSPESYTGTELEYAVDVCNAVADIVKPTP
EVPLIVNLPATVEMATPNVYADSIEWMNRHLSPRDSIILSLHPHNDRGTAVAAAELGYQAGADRIEGCLFGNGERTGNVC
LVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPVHERHPYGGDLVYTAFSGSHQDAINKGLDAMKVAADEADSDVDD
ILWQVPYLPIDPKDVGRTYEAVIRVNSQSGKGGVAYIMKADHGLVLPRRLQIEFSQAIQKITDGEGGEVSPKEMWDVFAE
EYLSPVRPLERMRQKVDAAEVDGGTDTITAVVKVDGVEREIVGAGNGPLAAFCDALGAIGFDISVLDYSEHALSAGEEAQ
AAAYVEASVGGKTVWGVGIATSITTASLRAVVSAVNRAARS
>Mature_601_residues
MNTPESTDAFSSVRAITTPSGPPHPGQPAWNTQRASAMPVSRYRSFAEEVEPVTLPDRTWPDKVVDTAPMWCAVDLRDGN
QALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQTDFDFVREIIEQGAIPDDVTIQVLTQCRPELITRTFEACKGAPR
AIVHFYNSTSILQRRVVFRADREAVKQIAVDGARMCVEEAAKHPDTLWRFEYSPESYTGTELEYAVDVCNAVADIVKPTP
EVPLIVNLPATVEMATPNVYADSIEWMNRHLSPRDSIILSLHPHNDRGTAVAAAELGYQAGADRIEGCLFGNGERTGNVC
LVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPVHERHPYGGDLVYTAFSGSHQDAINKGLDAMKVAADEADSDVDD
ILWQVPYLPIDPKDVGRTYEAVIRVNSQSGKGGVAYIMKADHGLVLPRRLQIEFSQAIQKITDGEGGEVSPKEMWDVFAE
EYLSPVRPLERMRQKVDAAEVDGGTDTITAVVKVDGVEREIVGAGNGPLAAFCDALGAIGFDISVLDYSEHALSAGEEAQ
AAAYVEASVGGKTVWGVGIATSITTASLRAVVSAVNRAARS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=534, Percent_Identity=26.4044943820225, Blast_Score=115, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6324682, Length=575, Percent_Identity=45.0434782608696, Blast_Score=507, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6324225, Length=575, Percent_Identity=45.7391304347826, Blast_Score=506, Evalue=1e-144,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 65612; Mature: 65612

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPESTDAFSSVRAITTPSGPPHPGQPAWNTQRASAMPVSRYRSFAEEVEPVTLPDRTW
CCCCCCCHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
PDKVVDTAPMWCAVDLRDGNQALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQTDFD
CHHHHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHH
FVREIIEQGAIPDDVTIQVLTQCRPELITRTFEACKGAPRAIVHFYNSTSILQRRVVFRA
HHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHH
DREAVKQIAVDGARMCVEEAAKHPDTLWRFEYSPESYTGTELEYAVDVCNAVADIVKPTP
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCC
EVPLIVNLPATVEMATPNVYADSIEWMNRHLSPRDSIILSLHPHNDRGTAVAAAELGYQA
CCCEEEECCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEHHHHCHHC
GADRIEGCLFGNGERTGNVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPVHER
CHHHHCEEEECCCCCCCCEEEEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
HPYGGDLVYTAFSGSHQDAINKGLDAMKVAADEADSDVDDILWQVPYLPIDPKDVGRTYE
CCCCCCEEEEEECCCCHHHHHCCCHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHCCCEE
AVIRVNSQSGKGGVAYIMKADHGLVLPRRLQIEFSQAIQKITDGEGGEVSPKEMWDVFAE
EEEEECCCCCCCCEEEEEECCCCEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
EYLSPVRPLERMRQKVDAAEVDGGTDTITAVVKVDGVEREIVGAGNGPLAAFCDALGAIG
HHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHCC
FDISVLDYSEHALSAGEEAQAAAYVEASVGGKTVWGVGIATSITTASLRAVVSAVNRAAR
CEEEEEECCHHHHCCCCCHHHHEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHC
S
C
>Mature Secondary Structure
MNTPESTDAFSSVRAITTPSGPPHPGQPAWNTQRASAMPVSRYRSFAEEVEPVTLPDRTW
CCCCCCCHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
PDKVVDTAPMWCAVDLRDGNQALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQTDFD
CHHHHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHH
FVREIIEQGAIPDDVTIQVLTQCRPELITRTFEACKGAPRAIVHFYNSTSILQRRVVFRA
HHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHH
DREAVKQIAVDGARMCVEEAAKHPDTLWRFEYSPESYTGTELEYAVDVCNAVADIVKPTP
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCC
EVPLIVNLPATVEMATPNVYADSIEWMNRHLSPRDSIILSLHPHNDRGTAVAAAELGYQA
CCCEEEECCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEHHHHCHHC
GADRIEGCLFGNGERTGNVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPVHER
CHHHHCEEEECCCCCCCCEEEEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
HPYGGDLVYTAFSGSHQDAINKGLDAMKVAADEADSDVDDILWQVPYLPIDPKDVGRTYE
CCCCCCEEEEEECCCCHHHHHCCCHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHCCCEE
AVIRVNSQSGKGGVAYIMKADHGLVLPRRLQIEFSQAIQKITDGEGGEVSPKEMWDVFAE
EEEEECCCCCCCCEEEEEECCCCEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
EYLSPVRPLERMRQKVDAAEVDGGTDTITAVVKVDGVEREIVGAGNGPLAAFCDALGAIG
HHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHCC
FDISVLDYSEHALSAGEEAQAAAYVEASVGGKTVWGVGIATSITTASLRAVVSAVNRAAR
CEEEEEECCHHHHCCCCCHHHHEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]