Click here to switch to the map view.

The map label for this gene is limB [H]

Identifier: 119870886

GI number: 119870886

Start: 5086556

End: 5087746

Strand: Direct

Name: limB [H]

Synonym: Mkms_4858

Alternate gene names: 119870886

Gene position: 5086556-5087746 (Clockwise)

Preceding gene: 119870885

Following gene: 119870887

Centisome position: 88.66

GC content: 67.42

Gene sequence:

>1191_bases
ATGAGCCGCAAACCGCTGAACTTCGGCGTCTTCATCACACCGTTCCACCCGGTGGGCCAATCCCCCACCGTCGCATTGGA
ATACGACCTCGAGCGGGTGGTGCGGCTGGACCGGCTCGGCTTCGACGAGGCGTGGTTCGGCGAACACCATTCGGGCGGTT
ACGAACTCATCGCCTGCCCGGAGGTCTTCATCGCCACGGCCGCCGAACGGACCAGACACATCCGCCTCGGCACCGGCGTG
GTGTCGCTGCCCTACCACCATCCGCTCATGGTCGCCGACCGGTGGGTCCTGCTCGACCACCTCACCCGTGGCCGCGTCAT
GTTCGGCACCGGGCCGGGCGCGCTGCCGTCGGACGCCTACATGATGGGCCTCGACCCGGTCGAGCAGCGCCGCATGATGC
AGGAGTCGCTCGAGGCGATCCTCGCGTTGTTCCGCGCGGAACCCGAGGAACGCATCACCCGCGAGACCGACTGGTTCACC
CTGCGCGACGCCCAACTTCACATCCGGCCCTACACCTGGCCGTACCCCGAGATCTCCACCGCCGCGATGATCTCCCCGTC
CGGACCACGACTGGCCGGTTCACTCGGGACGTCGCTGCTGTCACTGTCGATGTCGGTGCCGGGTGGATTCGCGGCACTGG
AGTCGACGTGGCAGATCGTCGTCGACCAGGCGGCCAAATCGGGCCGCCCCGAACCCGAACGGGACAACTGGCGGGTGCTG
TCGATCATGCACCTGGCCGACACCCGAGAGCAGGCGATCGACGACTGCACGTACGGACTGGCCGATTTCGCCAACTACTT
CGGTGCGGCCGGCTTCGTCCCGCTGTCCAACAGCGTCGAGGGGGAACGCTCACCGCGGGAGTTCGCGGCCGAGTACGCCG
CACAGGGGAACTGCTGTATCGGCACGCCCGACGACGCCATCGCCTACATCGACGACCTGCTCGTGAAGTCCGGCGGGTTC
GGGACACTGCTGCTGCTCGGCCACGACTGGGCGTCCCCGGAGGCCACCTACCACTCCTACGACCTGTTCGCGCGAAAGGT
GATGCCGCACTTCAAGGGTCAGCTCACCGCCCCTCGCGCCTCGCACGACTGGGCCAAGGGTATGCGCGACCAGTTGCTCG
GCCGGGCCGGCGACGCGATCGTCAAGGCGATCACCGAACACACCGACGAGTTGAAGGTGGACCCGCGGTAG

Upstream 100 bases:

>100_bases
GCGAGGTGCTGCTCGACGGTGCAGGCCACTGGCTCCAGCAGGAACGGCCCGACGAGGTCAACGCACTGTTGCTGGAGCAT
CTGGCCGGATTGGAGCTGAC

Downstream 100 bases:

>100_bases
TGCGCGCGTCGGTGCTGCGCGGCGGCCGCATGGTGTTGCGTGACGATGTGCCCGAACCGGTTCCCGGGCCGGGGCAGGTG
CTGGTCGCGGTGAAGGCGTG

Product: luciferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSRKPLNFGVFITPFHPVGQSPTVALEYDLERVVRLDRLGFDEAWFGEHHSGGYELIACPEVFIATAAERTRHIRLGTGV
VSLPYHHPLMVADRWVLLDHLTRGRVMFGTGPGALPSDAYMMGLDPVEQRRMMQESLEAILALFRAEPEERITRETDWFT
LRDAQLHIRPYTWPYPEISTAAMISPSGPRLAGSLGTSLLSLSMSVPGGFAALESTWQIVVDQAAKSGRPEPERDNWRVL
SIMHLADTREQAIDDCTYGLADFANYFGAAGFVPLSNSVEGERSPREFAAEYAAQGNCCIGTPDDAIAYIDDLLVKSGGF
GTLLLLGHDWASPEATYHSYDLFARKVMPHFKGQLTAPRASHDWAKGMRDQLLGRAGDAIVKAITEHTDELKVDPR

Sequences:

>Translated_396_residues
MSRKPLNFGVFITPFHPVGQSPTVALEYDLERVVRLDRLGFDEAWFGEHHSGGYELIACPEVFIATAAERTRHIRLGTGV
VSLPYHHPLMVADRWVLLDHLTRGRVMFGTGPGALPSDAYMMGLDPVEQRRMMQESLEAILALFRAEPEERITRETDWFT
LRDAQLHIRPYTWPYPEISTAAMISPSGPRLAGSLGTSLLSLSMSVPGGFAALESTWQIVVDQAAKSGRPEPERDNWRVL
SIMHLADTREQAIDDCTYGLADFANYFGAAGFVPLSNSVEGERSPREFAAEYAAQGNCCIGTPDDAIAYIDDLLVKSGGF
GTLLLLGHDWASPEATYHSYDLFARKVMPHFKGQLTAPRASHDWAKGMRDQLLGRAGDAIVKAITEHTDELKVDPR
>Mature_395_residues
SRKPLNFGVFITPFHPVGQSPTVALEYDLERVVRLDRLGFDEAWFGEHHSGGYELIACPEVFIATAAERTRHIRLGTGVV
SLPYHHPLMVADRWVLLDHLTRGRVMFGTGPGALPSDAYMMGLDPVEQRRMMQESLEAILALFRAEPEERITRETDWFTL
RDAQLHIRPYTWPYPEISTAAMISPSGPRLAGSLGTSLLSLSMSVPGGFAALESTWQIVVDQAAKSGRPEPERDNWRVLS
IMHLADTREQAIDDCTYGLADFANYFGAAGFVPLSNSVEGERSPREFAAEYAAQGNCCIGTPDDAIAYIDDLLVKSGGFG
TLLLLGHDWASPEATYHSYDLFARKVMPHFKGQLTAPRASHDWAKGMRDQLLGRAGDAIVKAITEHTDELKVDPR

Specific function: Acts on both enantiomers of limonene by their NAD- dependent epoxidation at the 1,2 double bond forming limonene-1,2- epoxide [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial luciferase oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1789551, Length=170, Percent_Identity=28.8235294117647, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011251
- InterPro:   IPR016048 [H]

Pfam domain/function: PF00296 Bac_luciferase [H]

EC number: =1.14.13.107 [H]

Molecular weight: Translated: 43898; Mature: 43767

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKPLNFGVFITPFHPVGQSPTVALEYDLERVVRLDRLGFDEAWFGEHHSGGYELIACP
CCCCCCCCCEEEECCCCCCCCCEEEEEECHHHHHHHHHCCCCHHHCCCCCCCCEEEEECC
EVFIATAAERTRHIRLGTGVVSLPYHHPLMVADRWVLLDHLTRGRVMFGTGPGALPSDAY
HHHHHHHHHHHEEEEECCCEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCE
MMGLDPVEQRRMMQESLEAILALFRAEPEERITRETDWFTLRDAQLHIRPYTWPYPEIST
EECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEECCEEEEECCCCCCCCCCC
AAMISPSGPRLAGSLGTSLLSLSMSVPGGFAALESTWQIVVDQAAKSGRPEPERDNWRVL
EEEECCCCCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
SIMHLADTREQAIDDCTYGLADFANYFGAAGFVPLSNSVEGERSPREFAAEYAAQGNCCI
EEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHCCCCEEE
GTPDDAIAYIDDLLVKSGGFGTLLLLGHDWASPEATYHSYDLFARKVMPHFKGQLTAPRA
CCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
SHDWAKGMRDQLLGRAGDAIVKAITEHTDELKVDPR
CCHHHHHHHHHHHHCCHHHHHHHHHHCCCCEECCCC
>Mature Secondary Structure 
SRKPLNFGVFITPFHPVGQSPTVALEYDLERVVRLDRLGFDEAWFGEHHSGGYELIACP
CCCCCCCCEEEECCCCCCCCCEEEEEECHHHHHHHHHCCCCHHHCCCCCCCCEEEEECC
EVFIATAAERTRHIRLGTGVVSLPYHHPLMVADRWVLLDHLTRGRVMFGTGPGALPSDAY
HHHHHHHHHHHEEEEECCCEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCE
MMGLDPVEQRRMMQESLEAILALFRAEPEERITRETDWFTLRDAQLHIRPYTWPYPEIST
EECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEECCEEEEECCCCCCCCCCC
AAMISPSGPRLAGSLGTSLLSLSMSVPGGFAALESTWQIVVDQAAKSGRPEPERDNWRVL
EEEECCCCCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
SIMHLADTREQAIDDCTYGLADFANYFGAAGFVPLSNSVEGERSPREFAAEYAAQGNCCI
EEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHCCCCEEE
GTPDDAIAYIDDLLVKSGGFGTLLLLGHDWASPEATYHSYDLFARKVMPHFKGQLTAPRA
CCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
SHDWAKGMRDQLLGRAGDAIVKAITEHTDELKVDPR
CCHHHHHHHHHHHHCCHHHHHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA