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The map label for this gene is otsA

Identifier: 119870726

GI number: 119870726

Start: 4920325

End: 4921794

Strand: Direct

Name: otsA

Synonym: Mkms_4697

Alternate gene names: 119870726

Gene position: 4920325-4921794 (Clockwise)

Preceding gene: 119870725

Following gene: 119870739

Centisome position: 85.76

GC content: 68.37

Gene sequence:

>1470_bases
GTGGCTTCGGAGGGCGACCCAGGCGTCGGCTCCGGAGACTCCGACTTCGTCGTGGTGGCCAACCGGCTGCCGATCGACAT
GGAACGGCTGCCCGACGGCAGCACGTCCTTCAAGCGGAGTCCCGGCGGTCTGGTCACCGCGCTCGAACCGCTGCTGCGCA
AACGCCACGGCGCGTGGATCGGCTGGGCCGGCATCCCGGACAGCGCGGAGGACCCGATCGAGGACGACGGTCTGCAGCTC
TACCCCGTCTCGCTGTCGGCCGACGACGTCGCCGACTACTACGAGGGCTTCTCCAATGCAACCCTGTGGCCGCTCTACCA
CGATCTGATCGTCAAACCGATCTACCACCGCAAGTGGTGGGACCGCTATGTCGAGGTCAACCGCCGCTTCGCCGAGGCGA
CCGCCCGCGCCGCGGCCGAGGGCGCCACGGTGTGGGTGCAGGACTACCAGCTGCAGTTGGTGCCGAAGATGCTGCGGATG
TTGCGGCCCGACCTGACGATCGGCTTCTTCCTGCACATTCCGTTCCCGCCGGTCGAGCTGTTCATGCAGATGCCGTGGCG
CACGGAGATCATCGAGGGCCTGCTCGGCGCCGACCTGGTCGGCTTCCACCTGCCCGGCGGGGCGCAGAACTTCCTCTACC
TGGCCCGTCGCCTGACCGGCGCCAACACCTCCCGCGCCACCGTGGGTGTGCGGTCGCGCTTCGGCGAGGTGCAGGTCGGG
TTCCGGACGGTCAAGGTCGGCGCGTTCCCCATCTCGATCGACTCCGACGAACTCGACGGCAAGGCCCGCAACCGCGCCGT
CCGCCAGCGCGCCCGCGAGATCCGCAACGAACTGGGGAATCCGCGCAAGATCCTGCTCGGGGTGGACCGCCTCGACTACA
CCAAGGGCATCAACGTGCGCCTCGAGGCGCTCTCGGAACTGCTCGAGGACGGCCGTGTCGACAGTCACGACACCGTGTTC
GTGCAGTTGGCTACCCCCAGCCGGGAACGCGTCCAGAGCTACATCGAGATGCGTGAGGACATCGAACGCCAGGTCGGCCA
CATCAACGGCGAGTTCGGCGACGTCGGCCACCCGATCGTGCACTACCTGCACCGGCCGATTCCCCGCGACGAGTTGATCG
CGTTCTTCGTGGCCGCCGACGTCATGTTGGTCACCCCCCTGCGCGACGGGATGAACCTGGTCGCCAAGGAGTACGTGGCC
TGCCGCAGCGATCTGGGGGGCGCACTGGTGCTCAGCGAATTCACCGGTGCCGCAGCCGAACTCCGTCAGGCGTACTTGGC
GAACCCGCACCACCTCGAAGGCGTCAAGGATGCCATCGAGGCGGCGCTGAACCAGGACCCCGAAGAGGGCCGCCGACGGA
TGCGCGCGCTGCGCAGGCAGGTCCTCGCCCACGACGTCGACCGGTGGGCCAGGGCGTTCCTCGACGCGCTCGCCGACACC
CGCGCAGGCGCCAAACCGGTCCGAGACTAG

Upstream 100 bases:

>100_bases
CCAAGCCCGCAAAGTCGTCGCCGCGTACGCCGTCGACGCCGACGAATGTCGCGTCTTCTTGTCCATGCTCGGCATCGGAC
CGTCGAAGCTCGAGGCGTAG

Downstream 100 bases:

>100_bases
ATCGGCGTGATGTAGTCGATCATCCACCTGCCGTCGACCTTGGCATAGTCGACCCGCAGCCGGCTGCCGTCGTAGATCGG
CTTGCCGTTCTTCTCGGTGA

Product: alpha,alpha-trehalose-phosphate synthase

Products: UDP; alpha,alpha'-Trehalose 6-phosphate

Alternate protein names: TPS; Trehalose-6-phosphate synthase

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MASEGDPGVGSGDSDFVVVANRLPIDMERLPDGSTSFKRSPGGLVTALEPLLRKRHGAWIGWAGIPDSAEDPIEDDGLQL
YPVSLSADDVADYYEGFSNATLWPLYHDLIVKPIYHRKWWDRYVEVNRRFAEATARAAAEGATVWVQDYQLQLVPKMLRM
LRPDLTIGFFLHIPFPPVELFMQMPWRTEIIEGLLGADLVGFHLPGGAQNFLYLARRLTGANTSRATVGVRSRFGEVQVG
FRTVKVGAFPISIDSDELDGKARNRAVRQRAREIRNELGNPRKILLGVDRLDYTKGINVRLEALSELLEDGRVDSHDTVF
VQLATPSRERVQSYIEMREDIERQVGHINGEFGDVGHPIVHYLHRPIPRDELIAFFVAADVMLVTPLRDGMNLVAKEYVA
CRSDLGGALVLSEFTGAAAELRQAYLANPHHLEGVKDAIEAALNQDPEEGRRRMRALRRQVLAHDVDRWARAFLDALADT
RAGAKPVRD

Sequences:

>Translated_489_residues
MASEGDPGVGSGDSDFVVVANRLPIDMERLPDGSTSFKRSPGGLVTALEPLLRKRHGAWIGWAGIPDSAEDPIEDDGLQL
YPVSLSADDVADYYEGFSNATLWPLYHDLIVKPIYHRKWWDRYVEVNRRFAEATARAAAEGATVWVQDYQLQLVPKMLRM
LRPDLTIGFFLHIPFPPVELFMQMPWRTEIIEGLLGADLVGFHLPGGAQNFLYLARRLTGANTSRATVGVRSRFGEVQVG
FRTVKVGAFPISIDSDELDGKARNRAVRQRAREIRNELGNPRKILLGVDRLDYTKGINVRLEALSELLEDGRVDSHDTVF
VQLATPSRERVQSYIEMREDIERQVGHINGEFGDVGHPIVHYLHRPIPRDELIAFFVAADVMLVTPLRDGMNLVAKEYVA
CRSDLGGALVLSEFTGAAAELRQAYLANPHHLEGVKDAIEAALNQDPEEGRRRMRALRRQVLAHDVDRWARAFLDALADT
RAGAKPVRD
>Mature_488_residues
ASEGDPGVGSGDSDFVVVANRLPIDMERLPDGSTSFKRSPGGLVTALEPLLRKRHGAWIGWAGIPDSAEDPIEDDGLQLY
PVSLSADDVADYYEGFSNATLWPLYHDLIVKPIYHRKWWDRYVEVNRRFAEATARAAAEGATVWVQDYQLQLVPKMLRML
RPDLTIGFFLHIPFPPVELFMQMPWRTEIIEGLLGADLVGFHLPGGAQNFLYLARRLTGANTSRATVGVRSRFGEVQVGF
RTVKVGAFPISIDSDELDGKARNRAVRQRAREIRNELGNPRKILLGVDRLDYTKGINVRLEALSELLEDGRVDSHDTVFV
QLATPSRERVQSYIEMREDIERQVGHINGEFGDVGHPIVHYLHRPIPRDELIAFFVAADVMLVTPLRDGMNLVAKEYVAC
RSDLGGALVLSEFTGAAAELRQAYLANPHHLEGVKDAIEAALNQDPEEGRRRMRALRRQVLAHDVDRWARAFLDALADTR
AGAKPVRD

Specific function: Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6- phosphate and nucleoside diphosphate

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family

Homologues:

Organism=Escherichia coli, GI1788206, Length=468, Percent_Identity=33.974358974359, Blast_Score=234, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI115533558, Length=545, Percent_Identity=27.5229357798165, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI115533560, Length=545, Percent_Identity=27.5229357798165, Blast_Score=186, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI71987012, Length=433, Percent_Identity=31.1778290993072, Blast_Score=165, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6319602, Length=480, Percent_Identity=37.5, Blast_Score=316, Evalue=5e-87,
Organism=Saccharomyces cerevisiae, GI6323537, Length=470, Percent_Identity=30, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6323917, Length=436, Percent_Identity=28.8990825688073, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6320279, Length=400, Percent_Identity=29.5, Blast_Score=185, Evalue=1e-47,
Organism=Drosophila melanogaster, GI19920676, Length=507, Percent_Identity=35.5029585798817, Blast_Score=284, Evalue=9e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OTSA_MYCSK (A1UM30)

Other databases:

- EMBL:   CP000518
- RefSeq:   YP_940678.1
- ProteinModelPortal:   A1UM30
- SMR:   A1UM30
- STRING:   A1UM30
- EnsemblBacteria:   EBMYCT00000056182
- GeneID:   4616112
- GenomeReviews:   CP000518_GR
- KEGG:   mkm:Mkms_4697
- eggNOG:   COG0380
- GeneTree:   EBGT00050000016525
- HOGENOM:   HBG559076
- OMA:   CHYRLDL
- ProtClustDB:   CLSK872185
- BioCyc:   MSP189918:MKMS_4697-MONOMER
- InterPro:   IPR001830

Pfam domain/function: PF00982 Glyco_transf_20

EC number: 2.4.1.15

Molecular weight: Translated: 54727; Mature: 54596

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00387 PPASE

Important sites: BINDING 22-22 BINDING 94-94 BINDING 148-148 BINDING 290-290 BINDING 295-295 BINDING 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASEGDPGVGSGDSDFVVVANRLPIDMERLPDGSTSFKRSPGGLVTALEPLLRKRHGAWI
CCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEE
GWAGIPDSAEDPIEDDGLQLYPVSLSADDVADYYEGFSNATLWPLYHDLIVKPIYHRKWW
EECCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHH
DRYVEVNRRFAEATARAAAEGATVWVQDYQLQLVPKMLRMLRPDLTIGFFLHIPFPPVEL
HHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHH
FMQMPWRTEIIEGLLGADLVGFHLPGGAQNFLYLARRLTGANTSRATVGVRSRFGEVQVG
HHHCCCHHHHHHHHHCCCHHEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEC
FRTVKVGAFPISIDSDELDGKARNRAVRQRAREIRNELGNPRKILLGVDRLDYTKGINVR
EEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCCEE
LEALSELLEDGRVDSHDTVFVQLATPSRERVQSYIEMREDIERQVGHINGEFGDVGHPIV
HHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
HYLHRPIPRDELIAFFVAADVMLVTPLRDGMNLVAKEYVACRSDLGGALVLSEFTGAAAE
HHHHCCCCHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHCCCCEEEHHHHCCHHHH
LRQAYLANPHHLEGVKDAIEAALNQDPEEGRRRMRALRRQVLAHDVDRWARAFLDALADT
HHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RAGAKPVRD
CCCCCCCCC
>Mature Secondary Structure 
ASEGDPGVGSGDSDFVVVANRLPIDMERLPDGSTSFKRSPGGLVTALEPLLRKRHGAWI
CCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEE
GWAGIPDSAEDPIEDDGLQLYPVSLSADDVADYYEGFSNATLWPLYHDLIVKPIYHRKWW
EECCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHH
DRYVEVNRRFAEATARAAAEGATVWVQDYQLQLVPKMLRMLRPDLTIGFFLHIPFPPVEL
HHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHH
FMQMPWRTEIIEGLLGADLVGFHLPGGAQNFLYLARRLTGANTSRATVGVRSRFGEVQVG
HHHCCCHHHHHHHHHCCCHHEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEC
FRTVKVGAFPISIDSDELDGKARNRAVRQRAREIRNELGNPRKILLGVDRLDYTKGINVR
EEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCCEE
LEALSELLEDGRVDSHDTVFVQLATPSRERVQSYIEMREDIERQVGHINGEFGDVGHPIV
HHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
HYLHRPIPRDELIAFFVAADVMLVTPLRDGMNLVAKEYVACRSDLGGALVLSEFTGAAAE
HHHHCCCCHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHCCCCEEEHHHHCCHHHH
LRQAYLANPHHLEGVKDAIEAALNQDPEEGRRRMRALRRQVLAHDVDRWARAFLDALADT
HHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RAGAKPVRD
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Monovalent cations [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose; D-glucose 6-phosphate

Specific reaction: UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA