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The map label for this gene is yfjR [H]

Identifier: 119870721

GI number: 119870721

Start: 4917007

End: 4917897

Strand: Direct

Name: yfjR [H]

Synonym: Mkms_4692

Alternate gene names: 119870721

Gene position: 4917007-4917897 (Clockwise)

Preceding gene: 119870720

Following gene: 119870722

Centisome position: 85.7

GC content: 69.81

Gene sequence:

>891_bases
ATGCACATCGGTTTCATCGGTCTGGGCAACATGGGTTCGGCCATGGCGGCGAACCTGCTGGCGGCGGGCCACGACGTCAC
CGCCTACAACAGGTCACCGGATAAGGTCGACGCGCTGAGCGCCGAGGGCGCCCGCCCGGCCCGCACGGTGGCAGAGGCGT
GCCAGGGAGACGTCGTTGTGACGATGCTCGCCAACGACGACGCCGTCGAGGCGGTCAGCTTCGGCGACGACGGCATCGTG
GCGTCGCTACGCCCCGACGCGATTCACGTCTCGTCGTCGACGATCAGTCTGGCGCTGGCCGAACGGCTCACCGCCGCACA
CGCCGAGGGCGGCAGCCGTTTCGTCTCTGCGCCCGTGTTCGGCAGGCCCGAGGCGGCCGCGGCCGCCAAACTCTTCGTCG
TGGCGGCCGGCGCACCCGATACGGTCGAAGCCCTGGCGCCCGTCTTCGAGGCGATCGGGCAGCGCACGTTCGTGGTCGGC
ACCGAACCGAAGGCCGCGAACCTGGTCAAGGTGTCGGGCAACTTCCTCATCGCCTCGGTCATCGAATCGCTCGGCGAGGC
CATGGCCCTGGTCGCCAAGGGTGGCGTCGACGTCGACCAGTACCTCGACCTTTTGACGTCCACCCTGTTCGACGCGCCGG
CCTACCGCACCTACGGCGGGTTGCTGGCGCGCCGCGAATTCGAACCCGCCGGATTCAGCGCGACGGGCGGACTCAAAGAC
GTCCGCCTGGCCCTGGAGGCCGGGGACGGGCTGCAGGTTCCGCTTCCGATCGCCAGCCTGCTGCGTGACCGGTTCCTCAC
CCTGCTCGCCACCGGCGGCGGCCATCTGGACTGGTCGGCCATCGGAGCGCTCAGCGCCTGGGAATCCGGAGGGCCTGGTC
CGAACGCCTGA

Upstream 100 bases:

>100_bases
GGCAGACCGTAAGCCCGTAGGCGAGGCGACCACGGCAGGTGTTTGCCGGTGCAGCGGCAATAATCCCGCGATCCCGGGCG
TTGGCGCGAAGATGAGCAAC

Downstream 100 bases:

>100_bases
GCGCGCCCACAATGACCTGATGGGACCGTCGACCAGTTACCGACTGCTGCAGATCGCGCTCGCCGTCTTCGGCACCGTCG
CGCTGCTGCTCTATCCGCTG

Product: 6-phosphogluconate dehydrogenase

Products: (R)-Glycerate; Nicotinamide adenine dinucleotide [C]

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MHIGFIGLGNMGSAMAANLLAAGHDVTAYNRSPDKVDALSAEGARPARTVAEACQGDVVVTMLANDDAVEAVSFGDDGIV
ASLRPDAIHVSSSTISLALAERLTAAHAEGGSRFVSAPVFGRPEAAAAAKLFVVAAGAPDTVEALAPVFEAIGQRTFVVG
TEPKAANLVKVSGNFLIASVIESLGEAMALVAKGGVDVDQYLDLLTSTLFDAPAYRTYGGLLARREFEPAGFSATGGLKD
VRLALEAGDGLQVPLPIASLLRDRFLTLLATGGGHLDWSAIGALSAWESGGPGPNA

Sequences:

>Translated_296_residues
MHIGFIGLGNMGSAMAANLLAAGHDVTAYNRSPDKVDALSAEGARPARTVAEACQGDVVVTMLANDDAVEAVSFGDDGIV
ASLRPDAIHVSSSTISLALAERLTAAHAEGGSRFVSAPVFGRPEAAAAAKLFVVAAGAPDTVEALAPVFEAIGQRTFVVG
TEPKAANLVKVSGNFLIASVIESLGEAMALVAKGGVDVDQYLDLLTSTLFDAPAYRTYGGLLARREFEPAGFSATGGLKD
VRLALEAGDGLQVPLPIASLLRDRFLTLLATGGGHLDWSAIGALSAWESGGPGPNA
>Mature_296_residues
MHIGFIGLGNMGSAMAANLLAAGHDVTAYNRSPDKVDALSAEGARPARTVAEACQGDVVVTMLANDDAVEAVSFGDDGIV
ASLRPDAIHVSSSTISLALAERLTAAHAEGGSRFVSAPVFGRPEAAAAAKLFVVAAGAPDTVEALAPVFEAIGQRTFVVG
TEPKAANLVKVSGNFLIASVIESLGEAMALVAKGGVDVDQYLDLLTSTLFDAPAYRTYGGLLARREFEPAGFSATGGLKD
VRLALEAGDGLQVPLPIASLLRDRFLTLLATGGGHLDWSAIGALSAWESGGPGPNA

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=285, Percent_Identity=29.8245614035088, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI23308751, Length=288, Percent_Identity=27.0833333333333, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1786719, Length=284, Percent_Identity=32.3943661971831, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI145693186, Length=289, Percent_Identity=31.1418685121107, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790315, Length=282, Percent_Identity=31.2056737588652, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789092, Length=284, Percent_Identity=29.2253521126761, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17557316, Length=273, Percent_Identity=27.8388278388278, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24655230, Length=304, Percent_Identity=30.9210526315789, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19922568, Length=304, Percent_Identity=30.9210526315789, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28574115, Length=282, Percent_Identity=24.822695035461, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.60 [C]

Molecular weight: Translated: 30139; Mature: 30139

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIGFIGLGNMGSAMAANLLAAGHDVTAYNRSPDKVDALSAEGARPARTVAEACQGDVVV
CEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCHHHHCCCCCCCHHHHHHHHCCCCEEE
TMLANDDAVEAVSFGDDGIVASLRPDAIHVSSSTISLALAERLTAAHAEGGSRFVSAPVF
EEEECCCCEEEEECCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCC
GRPEAAAAAKLFVVAAGAPDTVEALAPVFEAIGQRTFVVGTEPKAANLVKVSGNFLIASV
CCCCHHHHEEEEEEECCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEECCCCHHHHH
IESLGEAMALVAKGGVDVDQYLDLLTSTLFDAPAYRTYGGLLARREFEPAGFSATGGLKD
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCE
VRLALEAGDGLQVPLPIASLLRDRFLTLLATGGGHLDWSAIGALSAWESGGPGPNA
EEEEEECCCCCCCCHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MHIGFIGLGNMGSAMAANLLAAGHDVTAYNRSPDKVDALSAEGARPARTVAEACQGDVVV
CEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCHHHHCCCCCCCHHHHHHHHCCCCEEE
TMLANDDAVEAVSFGDDGIVASLRPDAIHVSSSTISLALAERLTAAHAEGGSRFVSAPVF
EEEECCCCEEEEECCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCC
GRPEAAAAAKLFVVAAGAPDTVEALAPVFEAIGQRTFVVGTEPKAANLVKVSGNFLIASV
CCCCHHHHEEEEEEECCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEECCCCHHHHH
IESLGEAMALVAKGGVDVDQYLDLLTSTLFDAPAYRTYGGLLARREFEPAGFSATGGLKD
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCE
VRLALEAGDGLQVPLPIASLLRDRFLTLLATGGGHLDWSAIGALSAWESGGPGPNA
EEEEEECCCCCCCCHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Hydroxy-3-oxopropanoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: 2-Hydroxy-3-oxopropanoate + H+ + Nicotinamide adenine dinucleotide - reduced --> (R)-Glycerate + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]